FastQCFastQC Report
Sat 18 Jun 2016
SRR1931145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933358
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.1966006194947906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42540.14502150777368464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35900.049.7416231
GTATCAA67500.041.8459851
ATCAACG77550.035.061633
TCAACGC79250.034.3744284
CAACGCA78000.034.3423465
AACGCAG82050.033.2111786
TATCAAC84650.032.972142
ACGCAGA91700.029.2549727
CGCAGAG91200.028.9971318
GCAGAGT103250.024.3231019
GTACATG66450.022.262251
GAGTACT63600.022.10386712-13
AGAGTAC89900.021.66552710-11
TACATGG67900.020.5882132
CAGAGTA103350.020.18060710-11
ACATGGG68600.019.1921673
GTACTTT74700.019.18507414-15
AGTACTT71800.018.65184612-13
GTGGTAT20750.018.2269741
GTGTAGC18800.016.297751
Produced by FastQC (version 0.11.5)