##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931145_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2933358 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.120231488962478 31.0 28.0 33.0 25.0 34.0 2 28.848758999072054 31.0 28.0 33.0 22.0 34.0 3 28.900485382282014 31.0 28.0 34.0 22.0 34.0 4 32.10215698186175 35.0 32.0 37.0 20.0 37.0 5 32.23765391063757 35.0 32.0 37.0 22.0 37.0 6 32.498081038863994 35.0 32.0 37.0 25.0 37.0 7 32.233485991140526 35.0 32.0 37.0 22.0 37.0 8 32.3909205763497 35.0 32.0 37.0 24.0 37.0 9 33.439621416819904 37.0 32.0 39.0 22.0 39.0 10-11 33.4249426766184 37.0 32.0 39.0 22.5 39.0 12-13 33.430675867043846 37.0 32.0 39.0 23.0 39.0 14-15 34.25984912172329 38.0 32.0 40.0 22.5 41.0 16-17 34.266060944487506 38.0 32.0 40.0 23.0 41.0 18-19 34.269842276326315 38.0 32.0 40.0 22.5 41.0 20-21 34.4839180897797 38.0 33.0 40.0 24.5 41.0 22-23 34.38574186989791 38.0 33.0 40.0 24.0 41.0 24-25 34.300684403335694 38.0 32.5 40.0 23.5 41.0 26-27 33.97025695465743 38.0 32.0 40.0 21.5 41.0 28-29 33.91709978802451 38.0 32.0 40.0 22.0 41.0 30-31 33.62983669228236 38.0 32.0 40.0 19.0 41.0 32-33 33.418489662700566 37.0 31.0 40.0 18.5 41.0 34-35 33.432188297507494 37.0 31.0 40.0 20.0 41.0 36-37 33.07724900949697 37.0 31.0 40.0 17.0 41.0 38-39 32.920902937861655 37.0 31.0 40.0 16.5 41.0 40-41 32.82815292235043 37.0 30.5 40.0 15.5 41.0 42-43 33.06464792227884 37.5 31.0 40.0 16.0 41.0 44-45 33.05982273558154 37.5 31.0 40.0 16.0 41.0 46-47 32.82164979521763 37.0 31.0 40.0 14.5 41.0 48-49 32.61410727909788 37.0 30.5 40.0 12.5 41.0 50-51 32.14323294326843 36.5 30.0 39.5 9.0 40.5 52-53 32.10100523018329 37.0 30.0 39.5 8.5 40.5 54-55 32.10759903837172 37.0 30.0 40.0 8.0 41.0 56-57 31.89764444026266 36.5 30.0 40.0 7.5 41.0 58-59 31.713384455630717 36.0 30.0 40.0 7.0 41.0 60-61 31.558453485732052 36.0 30.0 40.0 5.5 41.0 62-63 31.409289796881254 36.0 30.0 39.5 2.0 41.0 64-65 31.09893405441818 35.0 29.0 39.0 2.0 41.0 66-67 30.870190921121797 35.0 29.0 39.0 2.0 40.0 68-69 30.69725192083612 35.0 29.0 39.0 2.0 40.0 70-71 30.42272968386402 35.0 29.0 38.5 2.0 40.0 72-73 30.08280015599869 34.5 29.0 38.0 2.0 40.0 74-75 29.686334739912414 34.0 29.0 37.0 2.0 40.0 76-77 29.355873030158612 34.0 28.5 37.0 2.0 39.0 78-79 28.939291249141768 34.0 28.0 36.0 2.0 39.0 80-81 28.525488535664586 34.0 28.0 36.0 2.0 38.5 82-83 28.178385147670348 33.5 27.5 35.5 2.0 37.0 84-85 27.83173959673521 33.0 27.0 35.0 2.0 37.0 86-87 27.49013025345014 33.0 26.5 35.0 2.0 36.5 88-89 27.13558607575345 33.0 26.0 35.0 2.0 36.0 90-91 26.752023448893723 33.0 25.5 35.0 2.0 36.0 92-93 26.393774813711794 33.0 25.0 35.0 2.0 35.5 94-95 26.090674407965206 32.5 25.0 35.0 2.0 35.0 96-97 25.705870882449396 32.0 23.5 35.0 2.0 35.0 98-99 25.350927333111063 32.0 21.5 35.0 2.0 35.0 100-101 24.74332266978664 32.0 18.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.997871362482911 1101 2 5.6225397109202575 1101 3 5.146822454449282 1101 4 6.59595645219958 1101 5 5.935245245130229 1101 6 5.470215622728116 1101 7 6.109051150829821 1101 8 5.566681338919302 1101 9 6.24226475564619 1101 10-11 6.2724380347892605 1101 12-13 6.134223912371372 1101 14-15 6.434156983576635 1101 16-17 6.3364242395861865 1101 18-19 6.408220586222637 1101 20-21 5.73482660858437 1101 22-23 5.7892938684762605 1101 24-25 5.742650129268405 1101 26-27 5.830375637012118 1101 28-29 5.7647155725078925 1101 30-31 5.851654979656924 1101 32-33 5.7392546576597105 1101 34-35 5.5175417488576315 1101 36-37 5.634123098230614 1101 38-39 5.461095129670529 1101 40-41 5.686212159400277 1101 42-43 5.551962700328517 1101 44-45 5.580380596132294 1101 46-47 5.52908609790844 1101 48-49 5.34974444473988 1101 50-51 5.459008382398508 1101 52-53 5.221824855860444 1101 54-55 5.428836313122133 1101 56-57 5.414600496993721 1101 58-59 5.294533706674493 1101 60-61 5.249104528215902 1101 62-63 5.1373602237881535 1101 64-65 5.121892649856775 1101 66-67 5.0624173005492 1101 68-69 5.133882130412186 1101 70-71 4.895551667028986 1101 72-73 4.68046124968431 1101 74-75 4.828476984103958 1101 76-77 4.754267555028989 1101 78-79 4.734882259737059 1101 80-81 4.64883920353417 1101 82-83 4.487577993802745 1101 84-85 4.478863312170585 1101 86-87 4.278436648948517 1101 88-89 4.349612132754455 1101 90-91 4.257059430530692 1101 92-93 4.101224627441432 1101 94-95 4.0500184076236465 1101 96-97 4.045720851373012 1101 98-99 3.7935414116985875 1101 100-101 3.717860753742219 1102 1 5.187339201166061 1102 2 5.7029129017165765 1102 3 5.421175165354391 1102 4 6.843143125522822 1102 5 6.333309386385427 1102 6 5.7484871679919 1102 7 6.348000747505537 1102 8 5.953664808014032 1102 9 6.644318888988941 1102 10-11 6.686269341791245 1102 12-13 6.651538792223025 1102 14-15 6.867252687840825 1102 16-17 6.792681583589776 1102 18-19 6.843549902784812 1102 20-21 6.0571372166271615 1102 22-23 6.21204185198884 1102 24-25 6.012644060760394 1102 26-27 6.223082998305969 1102 28-29 6.181939116523264 1102 30-31 6.274706170808496 1102 32-33 6.085324157989735 1102 34-35 6.083313165977202 1102 36-37 6.21223023027866 1102 38-39 5.992685526352087 1102 40-41 6.106439805839635 1102 42-43 6.099463382256751 1102 44-45 6.0161493552225735 1102 46-47 5.903590648820419 1102 48-49 5.652126985742175 1102 50-51 5.788991964528165 1102 52-53 5.565794013876232 1102 54-55 5.949595223701323 1102 56-57 5.838451911079375 1102 58-59 5.826476123052942 1102 60-61 5.754771249669105 1102 62-63 5.557669363247985 1102 64-65 5.663789359120031 1102 66-67 5.67769689179562 1102 68-69 5.658316954120249 1102 70-71 5.482918148294704 1102 72-73 5.394801780021119 1102 74-75 5.4220800181837525 1102 76-77 5.273488835531747 1102 78-79 5.21913130883998 1102 80-81 5.404899218847152 1102 82-83 5.1965156290116425 1102 84-85 5.15269403892216 1102 86-87 5.122461721394387 1102 88-89 5.142343286995132 1102 90-91 5.166180206436714 1102 92-93 5.138935493353941 1102 94-95 5.108358783584372 1102 96-97 4.968388732187968 1102 98-99 4.687970796731651 1102 100-101 4.7073558092516095 1103 1 4.924738588160018 1103 2 5.440311287851664 1103 3 5.147116319656938 1103 4 6.649288839364729 1103 5 6.168275231742857 1103 6 5.574869514229288 1103 7 6.136405437183242 1103 8 5.691885633244858 1103 9 6.197195464140627 1103 10-11 6.227402472835429 1103 12-13 6.345607128731519 1103 14-15 6.521680774396167 1103 16-17 6.338080408501398 1103 18-19 6.250068750158373 1103 20-21 5.6208731772461675 1103 22-23 5.871966321840571 1103 24-25 5.656251395000822 1103 26-27 5.8360389295557695 1103 28-29 5.674168681787538 1103 30-31 5.960641061068994 1103 32-33 5.733348482596828 1103 34-35 5.685870407097717 1103 36-37 5.759051342368981 1103 38-39 5.359624408838446 1103 40-41 5.467204957108102 1103 42-43 5.482541614457077 1103 44-45 5.346122215933441 1103 46-47 5.305932044716947 1103 48-49 5.0588294573564845 1103 50-51 5.029146382372595 1103 52-53 4.791905114014963 1103 54-55 5.114501101598677 1103 56-57 5.187860650365508 1103 58-59 5.231442604956612 1103 60-61 4.993652041239045 1103 62-63 5.042605302942366 1103 64-65 4.921918301503119 1103 66-67 5.093700278168249 1103 68-69 5.068160421127406 1103 70-71 4.863986755969236 1103 72-73 4.737168442933452 1103 74-75 4.861925197326812 1103 76-77 4.91201043887029 1103 78-79 4.870196776486619 1103 80-81 4.948232504584087 1103 82-83 4.785970926917951 1103 84-85 4.690840373563461 1103 86-87 4.687115069253963 1103 88-89 4.782040999592237 1103 90-91 4.711835918458387 1103 92-93 4.557892614424766 1103 94-95 4.508302421885183 1103 96-97 4.384170428320811 1103 98-99 4.184912605729345 1103 100-101 4.332254288800193 1104 1 4.630933541082044 1104 2 5.445119908275853 1104 3 4.98775197589978 1104 4 6.57246824604232 1104 5 6.075980461422052 1104 6 5.650667844218798 1104 7 6.206884650109188 1104 8 5.709356042285663 1104 9 6.387008118777864 1104 10-11 6.452556712771191 1104 12-13 6.418548260223421 1104 14-15 6.4609561147120615 1104 16-17 6.351822785245574 1104 18-19 6.452786783286076 1104 20-21 5.8837333874004045 1104 22-23 5.9846504601537625 1104 24-25 5.71170692565029 1104 26-27 5.990277595266839 1104 28-29 5.932363673657893 1104 30-31 6.077997647205105 1104 32-33 5.9136242843005995 1104 34-35 5.833095589523083 1104 36-37 5.909985424864924 1104 38-39 5.671941341929905 1104 40-41 5.931599754158661 1104 42-43 5.858357461424276 1104 44-45 5.815358785078182 1104 46-47 5.727687850948627 1104 48-49 5.608679148075499 1104 50-51 5.594030225173505 1104 52-53 5.238745987822643 1104 54-55 5.5777051740305765 1104 56-57 5.570764148524518 1104 58-59 5.592566465457509 1104 60-61 5.213598945159639 1104 62-63 5.186358466679838 1104 64-65 5.400765337874077 1104 66-67 5.384125255222607 1104 68-69 5.273837012843945 1104 70-71 5.1944767311387 1104 72-73 5.157795301283208 1104 74-75 5.281350617011324 1104 76-77 5.1767017414612795 1104 78-79 5.158508464840597 1104 80-81 5.110238544701126 1104 82-83 4.856334141564396 1104 84-85 4.86504595143289 1104 86-87 4.912021339257318 1104 88-89 4.982375971490821 1104 90-91 5.016514029420222 1104 92-93 5.0216832402006375 1104 94-95 4.871235751336712 1104 96-97 4.831344333123802 1104 98-99 4.661737097141042 1104 100-101 4.731152002285878 1105 1 4.458876339655909 1105 2 5.01971357876409 1105 3 4.683113818861106 1105 4 6.29752607505462 1105 5 3.7075740781934527 1105 6 4.804091933985308 1105 7 5.505168622339248 1105 8 0.9996214418355116 1105 9 0.7371026487355117 1105 10-11 2.0956023382186935 1105 12-13 5.452047757086827 1105 14-15 3.564315026936079 1105 16-17 5.704977944664471 1105 18-19 6.164059549549766 1105 20-21 3.4386098086445074 1105 22-23 5.290214982417556 1105 24-25 5.400494607511817 1105 26-27 5.61046856196306 1105 28-29 3.0270139103122595 1105 30-31 5.333541721203556 1105 32-33 5.561038052093057 1105 34-35 5.52935923309952 1105 36-37 5.600541487560815 1105 38-39 5.412200639947589 1105 40-41 5.635195141417029 1105 42-43 2.958915737020355 1105 44-45 4.998789630320978 1105 46-47 5.253829433853731 1105 48-49 5.208558706416323 1105 50-51 5.27349059115533 1105 52-53 5.15406560467812 1105 54-55 5.384894255299145 1105 56-57 5.313942777363856 1105 58-59 5.329318445973982 1105 60-61 5.271329740722699 1105 62-63 4.880830520244533 1105 64-65 5.005872674793437 1105 66-67 5.143316256284258 1105 68-69 5.182772979870482 1105 70-71 4.923010056264367 1105 72-73 4.922692861437724 1105 74-75 4.97076259029437 1105 76-77 4.847074557171155 1105 78-79 4.86236695200094 1105 80-81 4.912424015097141 1105 82-83 4.676521655035316 1105 84-85 4.629771350789223 1105 86-87 4.59948267814784 1105 88-89 4.577860091158591 1105 90-91 4.671065797438214 1105 92-93 4.670917896202681 1105 94-95 4.575127894932162 1105 96-97 4.535509210619384 1105 98-99 4.605641829160749 1105 100-101 4.7202494234439065 1106 1 4.116220642978938 1106 2 4.317999464988091 1106 3 4.361182851922642 1106 4 6.122369646679978 1106 5 5.819303891737473 1106 6 5.028843184777667 1106 7 5.758996735731486 1106 8 5.331124208847292 1106 9 6.001393852086984 1106 10-11 5.891868939542768 1106 12-13 6.1100389688752195 1106 14-15 5.839809604160422 1106 16-17 5.931679183734737 1106 18-19 6.121056142489184 1106 20-21 5.462332305585193 1106 22-23 5.520873571913516 1106 24-25 5.519667601558929 1106 26-27 5.743094316899601 1106 28-29 5.757910254885115 1106 30-31 5.808679236500144 1106 32-33 5.648123793620105 1106 34-35 5.242215310408604 1106 36-37 5.496136723611709 1106 38-39 5.290957706803102 1106 40-41 5.485364481828917 1106 42-43 2.4074732613362357 1106 44-45 4.477469392944812 1106 46-47 4.610539698750678 1106 48-49 4.590367577839874 1106 50-51 4.88911627478867 1106 52-53 4.602833655398204 1106 54-55 4.846966370823758 1106 56-57 4.717179307593796 1106 58-59 4.846457507168537 1106 60-61 4.657450845272585 1106 62-63 4.61856347822194 1106 64-65 4.749999307767162 1106 66-67 4.908953587349828 1106 68-69 4.769605310417141 1106 70-71 4.5854470780010175 1106 72-73 4.384284917030321 1106 74-75 4.553348041195591 1106 76-77 4.381753643347967 1106 78-79 4.530416160162208 1106 80-81 4.482248248065201 1106 82-83 4.596641782246181 1106 84-85 4.623051888340619 1106 86-87 4.587451197975728 1106 88-89 4.678437231691582 1106 90-91 4.764990298931849 1106 92-93 4.760537117564368 1106 94-95 4.735808655614015 1106 96-97 4.646379489396196 1106 98-99 4.60345704899483 1106 100-101 4.654944730163354 1107 1 3.3351193383694735 1107 2 -0.01701507413804748 1107 3 1.510763475126133 1107 4 3.256846544270232 1107 5 3.22189373895462 1107 6 4.184807582826732 1107 7 5.293847228365216 1107 8 4.7658822797347185 1107 9 5.25829098103582 1107 10-11 2.8306534215476553 1107 12-13 5.241106123952111 1107 14-15 3.8809165149177645 1107 16-17 2.405466802531084 1107 18-19 3.2173461384960653 1107 20-21 2.797593014067111 1107 22-23 4.4750172975758 1107 24-25 4.507951013024055 1107 26-27 3.882617820383402 1107 28-29 4.63284449797354 1107 30-31 4.156221554449186 1107 32-33 4.500037504613378 1107 34-35 4.757071065894117 1107 36-37 5.047363091449338 1107 38-39 4.857158144674212 1107 40-41 3.7094684809296083 1107 42-43 4.093631240836402 1107 44-45 4.555878259976502 1107 46-47 4.720667554324784 1107 48-49 2.6729390633135033 1107 50-51 4.0724784983788 1107 52-53 4.077416410792299 1107 54-55 4.6749801216715525 1107 56-57 3.5603848686227586 1107 58-59 2.960847291841425 1107 60-61 3.8716047747983424 1107 62-63 4.542950635300365 1107 64-65 4.754545107489598 1107 66-67 4.869007484837169 1107 68-69 4.741037059742943 1107 70-71 4.532119937279731 1107 72-73 4.523013858630826 1107 74-75 4.701363716953651 1107 76-77 4.510763933725126 1107 78-79 4.444200448679894 1107 80-81 4.5770967452903655 1107 82-83 4.415051441617521 1107 84-85 4.348618152571795 1107 86-87 4.412720304185182 1107 88-89 4.438187396423903 1107 90-91 4.572108313632125 1107 92-93 4.6528558980295 1107 94-95 4.5914091214852135 1107 96-97 4.575667811298203 1107 98-99 4.529791338627465 1107 100-101 4.743662556786411 1108 1 -1.7492807954406473 1108 2 -0.9568804604983789 1108 3 -1.7353807380779962 1108 4 -3.5406140970781905 1108 5 -9.012697462373861 1108 6 -7.377198076772995 1108 7 -5.876670487810834 1108 8 -5.863601015317695 1108 9 -7.659240194864367 1108 10-11 -2.943519994841246 1108 12-13 -1.812318168925895 1108 14-15 -6.6749399873905695 1108 16-17 -5.4841015149706 1108 18-19 -4.037023803311833 1108 20-21 -4.358394510653323 1108 22-23 -3.2282398966314503 1108 24-25 -8.204445090084079 1108 26-27 -9.411748393544439 1108 28-29 -6.295148587956827 1108 30-31 -5.994563473138218 1108 32-33 -2.481914595407506 1108 34-35 -0.7951519832275338 1108 36-37 -4.7118932702709095 1108 38-39 -5.662442059320632 1108 40-41 -3.397041479944054 1108 42-43 -4.060806371286972 1108 44-45 -1.438007019160814 1108 46-47 -2.210903845510572 1108 48-49 -1.1531888117183335 1108 50-51 -0.5122288302398417 1108 52-53 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-3.5840986477086254 2313 56-57 -2.2274563439609167 2313 58-59 -2.770050006205814 2313 60-61 -1.15715116498869 2313 62-63 -3.2161194628578684 2313 64-65 -3.808570549268751 2313 66-67 -5.833829738984413 2313 68-69 -5.689343867037259 2313 70-71 -5.719690180302038 2313 72-73 -4.988182856710296 2313 74-75 -6.408303394835741 2313 76-77 -5.391171049452705 2313 78-79 -7.126966437657728 2313 80-81 -9.797792696629791 2313 82-83 -8.503142082713925 2313 84-85 -8.410096425486547 2313 86-87 -7.589685959905555 2313 88-89 -9.832593186975913 2313 90-91 -9.215487936931504 2313 92-93 -9.015599930765893 2313 94-95 -9.516478221999233 2313 96-97 -9.584220850211977 2313 98-99 -9.243293622005186 2313 100-101 -8.507054986829836 2314 1 -14.243626637930982 2314 2 -14.691785320431059 2314 3 -14.10651741151389 2314 4 -25.23151667587475 2314 5 -19.657615675298445 2314 6 -18.221729609307005 2314 7 -18.003649095205052 2314 8 -14.662113408563306 2314 9 -15.736823164235942 2314 10-11 -14.8180262123281 2314 12-13 -15.326999505337833 2314 14-15 -17.5576509924814 2314 16-17 -18.222137721051773 2314 18-19 -16.285106049396347 2314 20-21 -18.227801206622388 2314 22-23 -17.413269245394908 2314 24-25 -18.471014623007548 2314 26-27 -17.746131304496675 2314 28-29 -18.593127209260413 2314 30-31 -19.405701746151266 2314 32-33 -17.266717569230078 2314 34-35 -18.300846130166082 2314 36-37 -17.566695131344154 2314 38-39 -16.758441030027505 2314 40-41 -18.43969455252313 2314 42-43 -18.79579990343152 2314 44-45 -16.149427652315183 2314 46-47 -15.646764278926433 2314 48-49 -15.169424519844188 2314 50-51 -15.505378467205375 2314 52-53 -15.422081668574128 2314 54-55 -15.047911533851607 2314 56-57 -13.677368082707607 2314 58-59 -13.081612230389398 2314 60-61 -13.099174554220816 2314 62-63 -15.588802604958488 2314 64-65 -14.017253250063103 2314 66-67 -14.463132475083263 2314 68-69 -16.725586144177594 2314 70-71 -17.007587355941933 2314 72-73 -15.809950288307828 2314 74-75 -14.157696598719237 2314 76-77 -14.573375374254116 2314 78-79 -14.46903395751651 2314 80-81 -14.600694285332352 2314 82-83 -14.927402894717456 2314 84-85 -14.682382391947272 2314 86-87 -14.98013167923477 2314 88-89 -13.941044201980326 2314 90-91 -13.416227124927975 2314 92-93 -13.407700548594665 2314 94-95 -12.881879810701793 2314 96-97 -12.760522703698296 2314 98-99 -13.103454698792476 2314 100-101 -12.47279858788897 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80604.0 3 13601.0 4 9362.0 5 7494.0 6 8099.0 7 9359.0 8 10609.0 9 12431.0 10 13836.0 11 15471.0 12 17150.0 13 18624.0 14 20741.0 15 23139.0 16 25172.0 17 26604.0 18 24774.0 19 22580.0 20 22629.0 21 24439.0 22 27924.0 23 33994.0 24 42001.0 25 51202.0 26 60751.0 27 69872.0 28 78333.0 29 87857.0 30 100912.0 31 114097.0 32 132555.0 33 161222.0 34 195757.0 35 239971.0 36 299306.0 37 372540.0 38 360420.0 39 97801.0 40 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.82343870733226 20.301766144877483 14.421254571878489 23.453540575911767 2 14.2996185851202 20.833294832504283 41.648109267063994 23.218977315311523 3 17.609204195328356 26.144302877453075 31.034943569792713 25.211549357425856 4 11.271489087519193 17.920528076332527 40.767952127659576 30.0400307084887 5 12.24744234929054 37.91995850768431 36.38528266469228 13.447316478332871 6 29.04211651680098 38.743933229437985 19.076421090778304 13.137529162982728 7 25.511744379505995 32.87163516914807 23.590222260142486 18.026398191203448 8 24.14124739385405 36.18649646956598 21.66371511074904 18.008541025830922 9 24.89624205284599 16.520422082653475 21.932967639397067 36.65036822510347 10-11 23.058164900265133 26.99444126481544 30.24794731802467 19.69944651689476 12-13 24.11951816515059 24.69619693343348 29.677543999963103 21.506740901452826 14-15 21.408349317159676 26.402487964558485 27.47086005813738 24.71830266014446 16-17 20.82464079220343 29.36134332461477 28.15412321249357 21.65989267068823 18-19 20.609546489585274 28.527705874790403 30.067919938439925 20.7948276971844 20-21 21.595115452915575 27.784496081438242 30.089459137972725 20.530929327673462 22-23 21.194812905801573 27.721744352794747 29.806102453726567 21.27734028767711 24-25 21.112817700635837 27.790020785054686 29.830375390167656 21.266786124141817 26-27 21.04022238525982 27.920714553166086 29.7746421591217 21.26442090245239 28-29 20.959141029021158 28.037426397623904 29.821664887667055 21.181767685687884 30-31 21.206836908437836 27.86284405164971 29.720819810534906 21.209499229377553 32-33 21.068910773775357 27.97326332414423 29.620630369363766 21.337195532716645 34-35 21.42475261214603 28.050872194748912 29.58750198439455 20.93687320871051 36-37 21.212957089864325 27.775704807871538 29.62703952358941 21.384298578674727 38-39 21.145413022550848 27.996436105933682 29.682970457912596 21.175180413602877 40-41 21.407938542080824 27.786279471764953 29.51862286208656 21.28715912406766 42-43 21.270940209006625 27.982881804144128 29.360395222208403 21.385782764640847 44-45 21.37634341092704 28.09460083331826 29.26203802308874 21.26701773266596 46-47 21.309204818537086 28.067372846292898 29.10707263037835 21.516349704791665 48-49 21.287560678058643 28.176032486755336 29.149389593659624 21.387017241526397 50-51 21.231072321907778 28.30603785211478 28.909348794725858 21.553541031251587 52-53 21.325343570657516 28.368070727211837 28.859662248149558 21.446923453981093 54-55 21.258719520577692 28.285632309299068 28.83542633744912 21.62022183267412 56-57 21.329964127362828 28.32568420962618 28.690806941932507 21.65354472107849 58-59 21.28828202265987 28.361649335423778 28.817255137538694 21.532813504377653 60-61 21.37887066368967 28.3759914999533 28.68583070011844 21.559307136238587 62-63 21.257378062957198 28.33675603182428 28.758099079621374 21.64776682559715 64-65 21.18014575786522 28.284324654542676 28.75767294684113 21.777856640750976 66-67 21.23613960518968 28.279449695536652 28.700400701175923 21.78400999809774 68-69 21.30060497218546 28.25323400689585 28.698372309141945 21.747788711776742 70-71 21.34182053469096 28.069298053630003 28.646486381819063 21.942395029859977 72-73 21.343405748633476 28.119701038877626 28.655213581158524 21.88167963133037 74-75 21.254872402209347 28.301574509487082 28.572901773326 21.87065131497758 76-77 21.266923437234734 28.190132264796862 28.531703256131706 22.011241041836694 78-79 21.275105186615477 28.212887755262056 28.475556001006353 22.03645105711611 80-81 21.275514274084514 28.233222811535448 28.504703483175252 21.986559431204782 82-83 21.406507490732466 28.11184315040987 28.62018546662221 21.861463892235452 84-85 21.4393367601227 28.121968065268543 28.496794458773874 21.94190071583489 86-87 21.234861206848944 28.149138291337096 28.561532550749003 22.054467951064957 88-89 21.402552978531773 28.00445768978761 28.49693082126355 22.096058510417073 90-91 21.35852493967664 27.948088845616525 28.598026562049366 22.095359652657468 92-93 21.45680820411283 27.92004930867627 28.573259724861405 22.049882762349498 94-95 21.230344199378322 27.958350122964877 28.55347011854673 22.25783555911007 96-97 21.194617227082407 27.818783114778352 28.71909940757316 22.267500250566073 98-99 21.20331033579945 27.80296506597558 28.51680565413427 22.4769189440907 100-101 21.28768462628837 27.751590497988992 28.498703533629378 22.462021342093262 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 244.0 1 166.5 2 113.0 3 157.0 4 153.5 5 139.0 6 224.0 7 459.0 8 798.0 9 1052.5 10 1108.5 11 1117.5 12 1241.0 13 1454.5 14 1687.5 15 1982.5 16 2291.0 17 2504.5 18 2804.0 19 3215.0 20 3737.0 21 4459.0 22 5682.0 23 7590.5 24 9943.5 25 13299.5 26 18079.5 27 24101.5 28 31160.0 29 39241.0 30 47453.5 31 55896.0 32 65329.5 33 74050.5 34 81664.0 35 88827.0 36 96925.0 37 104028.5 38 107603.0 39 109932.0 40 111904.0 41 112867.5 42 113417.5 43 113201.5 44 113000.5 45 113397.5 46 111566.0 47 108715.0 48 105946.0 49 102020.5 50 97206.0 51 91654.5 52 85419.5 53 79258.5 54 73051.5 55 65714.5 56 58135.0 57 50657.0 58 43777.5 59 37725.0 60 31241.0 61 24769.5 62 19338.0 63 14834.5 64 10921.5 65 7954.0 66 5873.0 67 4314.5 68 3265.5 69 2469.0 70 1800.5 71 1382.0 72 1107.0 73 875.0 74 688.0 75 516.0 76 386.5 77 287.5 78 217.0 79 151.0 80 110.5 81 91.5 82 70.5 83 56.5 84 45.0 85 39.5 86 35.5 87 24.5 88 16.0 89 14.0 90 11.0 91 7.0 92 3.5 93 3.0 94 2.0 95 2.0 96 2.5 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025772510549343108 2 0.3866899301074059 3 0.0 4 0.5317455285035103 5 0.22332766747188718 6 0.09698782078423432 7 0.11021498228310353 8 0.11130588220053604 9 0.10980589481406633 10-11 0.1702485683643115 12-13 0.2160834102076869 14-15 0.12182283921703385 16-17 0.11602743340567362 18-19 0.16487929533319834 20-21 0.10695932784201587 22-23 0.09598214742285122 24-25 0.09841962692586448 26-27 0.16794745135097727 28-29 0.11387972419322837 30-31 0.12216374544123151 32-33 0.10486275456320025 34-35 0.14498741715126487 36-37 0.14045336436943598 38-39 0.1356124959858292 40-41 0.10987407605890587 42-43 0.11111838377722731 44-45 0.09085150874867642 46-47 0.09547078808655472 48-49 0.09998779555717373 50-51 0.08628336534442779 52-53 0.09458443190364081 54-55 0.3126791888340939 56-57 0.33478695747331216 58-59 0.3483038892627494 60-61 0.16716336703532264 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2933358.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.43946532480085 #Duplication Level Percentage of deduplicated Percentage of total 1 85.63265558474677 63.74449096071372 2 9.512459312231067 14.162047702528069 3 2.360126835015997 5.270597390919159 4 0.8911739073607504 2.6535403670139144 5 0.4446557577772233 1.6549968431265332 6 0.27933388573948387 1.2476079060927714 7 0.17574813922103508 0.9157818260809759 8 0.12453442142918594 0.7416220596577618 9 0.08551828116619155 0.5729341612956557 >10 0.45892400075108936 6.066010741939788 >50 0.025042266629388472 1.2557800992535393 >100 0.009363093024156673 1.1362688552842943 >500 3.251604355878694E-4 0.14564355266744955 >1k 9.290298137496447E-5 0.23326481583112704 >5k 4.645149068748224E-5 0.19941271759533202 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5767 0.1966006194947906 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4254 0.14502150777368464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 6.818124483953203E-5 0.0 0.0 3.409062241976602E-5 3 0.0 6.818124483953203E-5 0.0 0.0 3.409062241976602E-5 4 0.0 1.0227186725929805E-4 0.0 0.0 3.409062241976602E-5 5 0.0 1.0227186725929805E-4 0.0 0.0 3.409062241976602E-5 6 0.0 1.0227186725929805E-4 0.0 0.0 3.409062241976602E-5 7 0.0 1.0227186725929805E-4 0.0 0.0 3.409062241976602E-5 8 0.0 1.0227186725929805E-4 0.0 0.0 3.409062241976602E-5 9 0.0 2.045437345185961E-4 0.0 0.0 3.409062241976602E-5 10-11 0.0 2.045437345185961E-4 0.0 0.0 3.409062241976602E-5 12-13 0.0 2.045437345185961E-4 0.0 0.0 3.409062241976602E-5 14-15 0.0 2.045437345185961E-4 0.0 0.0 6.818124483953203E-5 16-17 0.0 2.045437345185961E-4 0.0 0.0 1.1931717846918105E-4 18-19 0.0 2.045437345185961E-4 0.0 3.409062241976602E-5 2.045437345185961E-4 20-21 0.0 2.045437345185961E-4 0.0 6.818124483953203E-5 3.0681560177789417E-4 22-23 0.0 2.5567966814824515E-4 0.0 2.0454373451859608E-4 3.0681560177789417E-4 24-25 1.704531120988301E-5 2.897702905680111E-4 0.0 4.772687138767242E-4 3.4090622419766014E-4 26-27 3.409062241976602E-5 3.0681560177789417E-4 0.0 9.034014941237995E-4 3.4090622419766014E-4 28-29 3.409062241976602E-5 3.2386091298777716E-4 0.0 0.002539751370272568 3.4090622419766014E-4 30-31 3.409062241976602E-5 3.749968466174262E-4 0.0 0.007636299422027587 3.4090622419766014E-4 32-33 5.1135933629649026E-5 3.749968466174262E-4 0.0 0.021272548389933994 3.4090622419766014E-4 34-35 6.818124483953203E-5 4.602234026668412E-4 0.0 0.04384054043181909 3.4090622419766014E-4 36-37 6.818124483953203E-5 5.454499587162563E-4 0.0 0.08059023140032687 3.4090622419766014E-4 38-39 6.818124483953203E-5 5.795405811360222E-4 0.0 0.13026026826592596 3.4090622419766014E-4 40-41 6.818124483953203E-5 6.136312035557883E-4 0.0 0.18177119874219239 3.4090622419766014E-4 42-43 6.818124483953203E-5 6.136312035557883E-4 0.0 0.23877071942804118 3.579515354075432E-4 44-45 8.522655604941505E-5 6.136312035557883E-4 0.0 0.3086394500773516 4.090874690371922E-4 46-47 1.1931717846918105E-4 6.136312035557883E-4 0.0 0.3912751188228645 4.090874690371922E-4 48-49 1.3636248967906407E-4 6.136312035557883E-4 0.0 0.4775584841672922 4.090874690371922E-4 50-51 1.3636248967906407E-4 6.477218259755543E-4 0.0 0.5661600118362642 4.090874690371922E-4 52-53 1.7045311209883007E-4 6.477218259755543E-4 0.0 0.6548126754388657 4.090874690371922E-4 54-55 1.7045311209883007E-4 6.477218259755543E-4 0.0 0.7540504773028045 4.090874690371922E-4 56-57 1.7045311209883007E-4 6.477218259755543E-4 0.0 0.8731801573486768 4.261327802470752E-4 58-59 1.7045311209883007E-4 6.477218259755543E-4 0.0 1.0052301832916406 4.431780914569582E-4 60-61 1.7045311209883007E-4 6.477218259755543E-4 0.0 1.1406722261653708 4.431780914569582E-4 62-63 1.7045311209883007E-4 6.647671371854373E-4 0.0 1.2752108675449776 4.431780914569582E-4 64-65 1.7045311209883007E-4 6.818124483953203E-4 0.0 1.4192778378909088 4.431780914569582E-4 66-67 1.7045311209883007E-4 6.818124483953203E-4 0.0 1.584003725423218 4.431780914569582E-4 68-69 1.7045311209883007E-4 6.818124483953203E-4 0.0 1.7728998642511415 4.431780914569582E-4 70-71 2.045437345185961E-4 6.818124483953203E-4 0.0 1.968511855695759 4.431780914569582E-4 72-73 2.045437345185961E-4 6.818124483953203E-4 0.0 2.169561301416329 4.772687138767242E-4 74-75 2.045437345185961E-4 7.329483820249694E-4 0.0 2.380582254194681 4.772687138767242E-4 76-77 2.045437345185961E-4 7.499936932348523E-4 0.0 2.608375793203557 4.772687138767242E-4 78-79 2.045437345185961E-4 7.499936932348523E-4 0.0 2.8631009239240486 4.772687138767242E-4 80-81 2.2158904572847912E-4 7.499936932348523E-4 0.0 3.1344452330741763 4.943140250866072E-4 82-83 2.386343569383621E-4 7.499936932348523E-4 0.0 3.414244016584406 5.113593362964902E-4 84-85 2.386343569383621E-4 7.499936932348523E-4 0.0 3.6950655187672288 5.113593362964902E-4 86-87 2.386343569383621E-4 7.840843156546183E-4 0.0 3.9960857147337627 5.113593362964902E-4 88-89 2.386343569383621E-4 8.011296268645013E-4 0.0 4.322145472867614 5.454499587162563E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3590 0.0 49.741623 1 GTATCAA 6750 0.0 41.845985 1 ATCAACG 7755 0.0 35.06163 3 TCAACGC 7925 0.0 34.374428 4 CAACGCA 7800 0.0 34.342346 5 AACGCAG 8205 0.0 33.211178 6 TATCAAC 8465 0.0 32.97214 2 ACGCAGA 9170 0.0 29.254972 7 CGCAGAG 9120 0.0 28.997131 8 GCAGAGT 10325 0.0 24.323101 9 GTACATG 6645 0.0 22.26225 1 GAGTACT 6360 0.0 22.103867 12-13 AGAGTAC 8990 0.0 21.665527 10-11 TACATGG 6790 0.0 20.588213 2 CAGAGTA 10335 0.0 20.180607 10-11 ACATGGG 6860 0.0 19.192167 3 GTACTTT 7470 0.0 19.185074 14-15 AGTACTT 7180 0.0 18.651846 12-13 GTGGTAT 2075 0.0 18.226974 1 GTGTAGC 1880 0.0 16.29775 1 >>END_MODULE