FastQCFastQC Report
Sat 18 Jun 2016
SRR1931145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933358
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75400.25704329304503576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60530.2063505375068437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39070.13319206179402582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39750.050.481361
GTATCAA73250.042.0652161
ATCAACG86900.034.756623
TCAACGC88600.034.142754
CAACGCA89150.033.825575
AACGCAG92100.032.7421266
TATCAAC94900.032.4271662
ACGCAGA105650.028.0933347
CGCAGAG105900.027.4888948
GCAGAGT116450.024.3459999
GAGTACT78150.021.42016812-13
GTGGTAT20250.021.1335871
GTACATG67800.020.6894071
AGAGTAC107400.020.31767510-11
CAGAGTA119950.019.61716310-11
GTACTTT86150.019.40350314-15
ACATGGG69700.018.9414043
TACATGG71400.018.7564682
TGGTATC21100.018.4557462
ACGACCG1300.00757599118.2649885
Produced by FastQC (version 0.11.5)