##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931145_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2933358 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.292099020985507 31.0 31.0 34.0 30.0 34.0 2 31.29343707791548 31.0 31.0 34.0 28.0 34.0 3 31.44944292513904 31.0 31.0 34.0 28.0 34.0 4 35.11954694926429 35.0 35.0 37.0 32.0 37.0 5 34.98667636203968 35.0 35.0 37.0 32.0 37.0 6 35.090153673707746 36.0 35.0 37.0 32.0 37.0 7 34.939756074778465 36.0 35.0 37.0 32.0 37.0 8 34.995238221860404 36.0 35.0 37.0 32.0 37.0 9 36.38609879871465 38.0 35.0 39.0 32.0 39.0 10-11 36.28330619719789 38.0 35.0 39.0 32.0 39.0 12-13 36.217097947130895 38.0 35.0 39.0 32.0 39.0 14-15 37.16213500022841 39.0 36.0 40.0 31.5 41.0 16-17 37.22158342077578 39.0 36.0 40.0 32.0 41.0 18-19 37.27847061286076 39.0 36.0 40.0 31.5 41.0 20-21 37.26840092481041 39.0 36.0 40.0 31.0 41.0 22-23 37.149220790643355 39.0 36.0 40.0 31.0 41.0 24-25 37.00770004888595 39.0 36.0 40.0 31.0 41.0 26-27 36.77256015119873 39.0 36.0 40.0 30.5 41.0 28-29 36.500574767893994 38.0 35.0 40.0 30.0 41.0 30-31 36.27378894768385 38.0 35.0 40.0 30.0 41.0 32-33 36.12401350261373 38.0 35.0 40.0 30.0 41.0 34-35 35.91129006415173 38.0 34.0 40.0 29.0 41.0 36-37 36.178201058309284 38.0 35.0 40.0 30.0 41.0 38-39 36.166810529093276 38.0 35.0 40.0 30.0 41.0 40-41 36.026034326529526 38.0 35.0 40.0 29.5 41.0 42-43 35.872753342755985 38.0 35.0 40.0 29.0 41.0 44-45 35.641730569538396 38.0 34.5 40.0 28.0 41.0 46-47 35.44679067471478 38.0 34.0 40.0 27.5 41.0 48-49 35.21430660696717 38.0 34.0 40.0 27.0 41.0 50-51 34.878958688301935 38.0 33.5 40.0 26.0 41.0 52-53 34.76400221179958 38.0 33.0 40.0 26.0 41.0 54-55 34.53145865591585 38.0 33.0 40.0 24.5 41.0 56-57 34.340643555951914 38.0 33.0 40.0 24.5 41.0 58-59 34.121467955837645 37.5 33.0 40.0 24.0 41.0 60-61 33.84586964836886 37.0 32.5 40.0 23.0 41.0 62-63 33.59454676176587 37.0 32.0 40.0 22.5 41.0 64-65 33.37380657253564 36.0 32.0 39.5 22.5 41.0 66-67 33.14864431821823 36.0 31.5 39.0 22.0 41.0 68-69 32.84508420042832 36.0 31.0 39.0 22.0 40.0 70-71 31.99995568219085 35.0 31.0 38.5 19.0 40.0 72-73 31.98733328833371 35.0 31.0 38.0 20.0 40.0 74-75 31.68934647595009 35.0 30.5 37.5 19.5 39.5 76-77 30.950541665899628 34.0 30.0 36.0 18.5 39.0 78-79 30.968958101943237 34.0 30.0 36.0 18.0 39.0 80-81 30.68573082453625 34.0 30.0 36.0 17.5 38.5 82-83 30.324266932300795 34.0 30.0 36.0 14.0 37.5 84-85 29.950111101338464 34.0 29.0 35.0 11.0 37.0 86-87 29.603232881905313 34.0 29.0 35.0 7.5 36.5 88-89 29.283572615412098 34.0 29.0 35.0 6.0 36.0 90-91 28.974146524222412 34.0 29.0 35.0 2.0 36.0 92-93 28.635471701715236 34.0 29.0 35.0 2.0 35.5 94-95 28.252705431795235 33.5 29.0 35.0 2.0 35.0 96-97 27.88722788012919 33.0 28.5 35.0 2.0 35.0 98-99 27.41737660387856 33.0 27.0 35.0 2.0 35.0 100-101 26.746116907653274 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8263316469240607 1101 2 0.6806642830749681 1101 3 0.5365063448121496 1101 4 0.7675205521551618 1101 5 0.7359198335779027 1101 6 0.7604545748033047 1101 7 0.6672453181986953 1101 8 0.697161808098592 1101 9 1.112643522341699 1101 10-11 0.8569564199932529 1101 12-13 0.9361222027029754 1101 14-15 1.231470483983614 1101 16-17 0.9945326004293378 1101 18-19 0.8325578200763033 1101 20-21 0.7779624784960433 1101 22-23 0.7683239388602345 1101 24-25 0.6190661494141452 1101 26-27 0.8297465711903911 1101 28-29 0.7866273868567077 1101 30-31 0.9596582385963259 1101 32-33 1.14994366925783 1101 34-35 0.8435370807733733 1101 36-37 1.0843771775554742 1101 38-39 0.9161605354803939 1101 40-41 1.02606953810173 1101 42-43 1.0565364064442164 1101 44-45 0.8663362579554885 1101 46-47 0.9480272115559458 1101 48-49 0.858361831263224 1101 50-51 0.7507944322805358 1101 52-53 0.6331975649101267 1101 54-55 0.6153368215933313 1101 56-57 0.5703316086716157 1101 58-59 0.7155262612261168 1101 60-61 0.9593451276018925 1101 62-63 0.643857182440577 1101 64-65 0.6609434557766889 1101 66-67 0.4599054220783927 1101 68-69 0.3065018383773648 1101 70-71 0.6034533417221084 1101 72-73 0.08838593605590361 1101 74-75 -0.12092963567564396 1101 76-77 -0.04091474991999178 1101 78-79 0.01785178490289141 1101 80-81 0.032596982448900746 1101 82-83 -0.14214495201417776 1101 84-85 -0.1234033127076124 1101 86-87 -0.16165743239074004 1101 88-89 -0.32824879328854806 1101 90-91 -0.16226133706455315 1101 92-93 -0.38136091285583973 1101 94-95 -0.3407142441176738 1101 96-97 -0.329821369465634 1101 98-99 -0.36348743848086684 1101 100-101 -0.698167744970192 1102 1 0.95037239953162 1102 2 0.8525547221496446 1102 3 0.6125606567862611 1102 4 0.8671337006829845 1102 5 0.9119388280012686 1102 6 0.8934943009840737 1102 7 0.9451528668879661 1102 8 0.951852506155241 1102 9 1.3124608573695085 1102 10-11 1.2249839927421462 1102 12-13 1.22667363221953 1102 14-15 1.5771109608315186 1102 16-17 1.356060765589774 1102 18-19 1.1911100912909731 1102 20-21 1.253514069826089 1102 22-23 0.9922798918240403 1102 24-25 0.5886261360161171 1102 26-27 0.965709744318552 1102 28-29 1.2491416694926656 1102 30-31 1.4704114143449871 1102 32-33 1.5116822254433941 1102 34-35 1.4487892280516448 1102 36-37 1.690776810215425 1102 38-39 1.5018999704840112 1102 40-41 1.6023301706376643 1102 42-43 1.7038479475109085 1102 44-45 1.7019552708986296 1102 46-47 1.7045590823455683 1102 48-49 1.5380004558660687 1102 50-51 1.5504769997961176 1102 52-53 1.544809213095327 1102 54-55 1.3070239003213118 1102 56-57 1.3821821980780555 1102 58-59 1.5129177106802416 1102 60-61 1.8405945199638012 1102 62-63 1.7113258822528579 1102 64-65 1.6192995172593783 1102 66-67 1.2884331351661444 1102 68-69 1.2280106254523275 1102 70-71 1.4991075509396339 1102 72-73 0.9262661499927525 1102 74-75 0.9044532374630592 1102 76-77 1.0223144032273197 1102 78-79 1.0253212190736498 1102 80-81 1.01575782752667 1102 82-83 0.9174474348056663 1102 84-85 0.9528540199053914 1102 86-87 0.8574170936716499 1102 88-89 0.8541790367270288 1102 90-91 0.7790411582601102 1102 92-93 0.6903358760209883 1102 94-95 0.8570396675305574 1102 96-97 0.953641926111441 1102 98-99 0.8730779387545979 1102 100-101 0.7244219762489692 1103 1 0.6242510772876244 1103 2 0.4771687909692339 1103 3 0.3728725724514881 1103 4 0.5993170783121116 1103 5 0.583914939768647 1103 6 0.675190300377686 1103 7 0.6629871977884605 1103 8 0.6513964748195491 1103 9 0.8885586260680896 1103 10-11 0.8345554529979111 1103 12-13 0.8539007767398203 1103 14-15 1.1521813145377138 1103 16-17 0.8529652759135686 1103 18-19 0.7737749621054419 1103 20-21 0.8358926282797157 1103 22-23 0.6236518264602182 1103 24-25 0.42305798980424925 1103 26-27 0.8279805209476692 1103 28-29 0.6876258807438091 1103 30-31 1.0453200292577307 1103 32-33 1.0966816662969023 1103 34-35 0.9778596743706487 1103 36-37 1.1242195618887294 1103 38-39 0.9573424341768586 1103 40-41 0.9729938161339788 1103 42-43 1.1485020867162348 1103 44-45 1.0131791091149935 1103 46-47 1.1304955259390326 1103 48-49 0.9194046848817905 1103 50-51 0.8924497246570766 1103 52-53 0.46276223918544446 1103 54-55 0.5317915809603306 1103 56-57 0.6357222692644768 1103 58-59 0.8525448226926216 1103 60-61 0.9904185556296099 1103 62-63 0.7195135750516428 1103 64-65 0.7535260348416699 1103 66-67 0.5232101718338811 1103 68-69 0.49930296378426675 1103 70-71 0.8623392855321441 1103 72-73 0.26857913350767504 1103 74-75 0.21859611393679046 1103 76-77 0.33956234426045384 1103 78-79 0.36196647225357736 1103 80-81 0.36161758798244037 1103 82-83 0.30487987015952456 1103 84-85 0.3048590538251652 1103 86-87 0.3035424620544376 1103 88-89 0.07739565301268314 1103 90-91 0.03380297786957698 1103 92-93 -0.172000957293573 1103 94-95 -0.18678937696682496 1103 96-97 -0.24668151144109984 1103 98-99 -0.4575093158548249 1103 100-101 -0.5944617017979503 1104 1 0.5463320825139952 1104 2 0.46367329321351747 1104 3 0.21727673989646945 1104 4 0.638332295254294 1104 5 0.4371538644404964 1104 6 0.6376986273737799 1104 7 0.6095817787865201 1104 8 0.5506796497413049 1104 9 0.8704905425680849 1104 10-11 0.7144101698748315 1104 12-13 0.75902677067117 1104 14-15 1.1513862280711251 1104 16-17 0.8721896785908143 1104 18-19 0.7875974173923765 1104 20-21 0.6910098653505017 1104 22-23 0.46690291499432135 1104 24-25 0.4830508214883338 1104 26-27 0.30997628209308203 1104 28-29 0.7255494698569294 1104 30-31 0.9745198182854509 1104 32-33 1.1352474306128855 1104 34-35 1.0060413782179864 1104 36-37 1.1282817161246257 1104 38-39 0.647978718933679 1104 40-41 0.908852450248709 1104 42-43 1.246354923391948 1104 44-45 1.1197691693715726 1104 46-47 1.1039531526337711 1104 48-49 0.9505347543192428 1104 50-51 0.9687587792443964 1104 52-53 0.6210224011166972 1104 54-55 0.8801506319133381 1104 56-57 0.9151805729306517 1104 58-59 0.8802004473130793 1104 60-61 1.1897673630422503 1104 62-63 0.2172612154212814 1104 64-65 -0.6357533794171744 1104 66-67 -0.004543751721627132 1104 68-69 0.45119542914565614 1104 70-71 1.0222237164658239 1104 72-73 0.6249295443148348 1104 74-75 0.4565773797912982 1104 76-77 0.30780149754205155 1104 78-79 0.3212656817718731 1104 80-81 0.3063090579600569 1104 82-83 0.16724893734578217 1104 84-85 0.1141719994676258 1104 86-87 0.19652046940589685 1104 88-89 0.32286996157566605 1104 90-91 0.4662179252737033 1104 92-93 0.5637496375476907 1104 94-95 0.5934524198510793 1104 96-97 0.7076193022532848 1104 98-99 0.605350532822456 1104 100-101 0.18338089758997356 1105 1 -0.2654464936761656 1105 2 -1.8334623564832988 1105 3 -0.40650941804171126 1105 4 0.3141808294518995 1105 5 0.549110848930674 1105 6 -0.01685706097246964 1105 7 -0.06514871115775378 1105 8 -0.19889703821858262 1105 9 0.6198137854769641 1105 10-11 0.8946902181065468 1105 12-13 0.9938574984042106 1105 14-15 1.244634717994046 1105 16-17 0.7965582680200072 1105 18-19 0.5977880312590642 1105 20-21 0.8933554603028497 1105 22-23 0.3283109075593771 1105 24-25 -0.09165374424272699 1105 26-27 0.8361394685091952 1105 28-29 0.528759251125976 1105 30-31 1.0834477236156985 1105 32-33 1.1286438670511885 1105 34-35 0.7523724305875632 1105 36-37 0.8025938152919068 1105 38-39 0.6696342368540797 1105 40-41 0.9857254162656304 1105 42-43 1.3361895789602514 1105 44-45 1.2243410686922047 1105 46-47 1.1823649517541526 1105 48-49 1.0347717650205652 1105 50-51 0.906178641896112 1105 52-53 0.9366900263453246 1105 54-55 0.01956765991749876 1105 56-57 0.1430093441696556 1105 58-59 -0.3655295645179706 1105 60-61 0.3384522138595756 1105 62-63 0.8432331648887512 1105 64-65 1.213321594108102 1105 66-67 0.9905856783327067 1105 68-69 0.8332770642261522 1105 70-71 0.2264128040736466 1105 72-73 -0.3508213903915198 1105 74-75 -0.21097357113019655 1105 76-77 0.07166661422770204 1105 78-79 -0.40478626324477673 1105 80-81 -0.21129615614325203 1105 82-83 -0.20338698660719245 1105 84-85 -0.11809343644951653 1105 86-87 -0.48111713161345193 1105 88-89 -0.8866923321066373 1105 90-91 -1.065796038268246 1105 92-93 -0.5450469576388848 1105 94-95 -0.7867920409753317 1105 96-97 -0.8849548495044566 1105 98-99 -1.6861996083427258 1105 100-101 -1.0029533469277396 1106 1 0.3240894550781164 1106 2 0.30617183014888383 1106 3 -0.2785501956272718 1106 4 0.36020241938613395 1106 5 0.06408069253403426 1106 6 0.36252332549828736 1106 7 0.4944420439915689 1106 8 0.5142094402176198 1106 9 0.9712630601629826 1106 10-11 0.3034997833362709 1106 12-13 0.3971960603271114 1106 14-15 -0.015079198484905021 1106 16-17 -0.1744969996058785 1106 18-19 0.47748099915918374 1106 20-21 0.49778483971869747 1106 22-23 0.4844767249963269 1106 24-25 0.29333248229534803 1106 26-27 0.7158034811541185 1106 28-29 0.7446564468573342 1106 30-31 0.31801487591558697 1106 32-33 0.14438887620114116 1106 34-35 -1.0426626041046134 1106 36-37 -0.768402090560997 1106 38-39 -0.7377839362013745 1106 40-41 0.47737566328825665 1106 42-43 0.5935521933906784 1106 44-45 0.48703557987710155 1106 46-47 0.101612898147593 1106 48-49 0.04770676114929273 1106 50-51 0.7582734484479445 1106 52-53 0.07664834384387831 1106 54-55 0.686424056224368 1106 56-57 0.43555254699698764 1106 58-59 0.8085463498614587 1106 60-61 0.980401957616067 1106 62-63 0.7366364344573739 1106 64-65 0.803020704908775 1106 66-67 0.625769458574041 1106 68-69 0.5541716196862296 1106 70-71 0.8613717786068307 1106 72-73 0.1448081159888126 1106 74-75 0.41147057345923344 1106 76-77 0.47171857969456 1106 78-79 0.5533383947052322 1106 80-81 0.6310679114163342 1106 82-83 0.44434510527910476 1106 84-85 0.5478908723687077 1106 86-87 0.5559109000600984 1106 88-89 0.49157956506595113 1106 90-91 0.5520446873891807 1106 92-93 0.14803683350324448 1106 94-95 0.29773880176118794 1106 96-97 0.6130856426816855 1106 98-99 0.6530671335344955 1106 100-101 0.4373873945955786 1107 1 0.5449871605804866 1107 2 0.4828870213613605 1107 3 0.31960846525434405 1107 4 -0.9563238624205752 1107 5 0.32053896952938743 1107 6 0.636441504091799 1107 7 0.7421775349613071 1107 8 0.7359360372130581 1107 9 1.058038735090392 1107 10-11 1.0599042670591743 1107 12-13 1.1059462947148262 1107 14-15 1.2914571285640903 1107 16-17 1.1405050628527107 1107 18-19 1.033507880163178 1107 20-21 0.4360268295977505 1107 22-23 0.8669410683708918 1107 24-25 0.6709468870609143 1107 26-27 1.0329706462003898 1107 28-29 0.8874185428915098 1107 30-31 1.2473310139969342 1107 32-33 1.256489458279603 1107 34-35 1.1358095658079534 1107 36-37 1.2938560360737341 1107 38-39 1.1896192839926485 1107 40-41 0.5680895498223038 1107 42-43 0.391054737268405 1107 44-45 -0.03006700259078343 1107 46-47 0.5267223563458927 1107 48-49 0.9959574414770955 1107 50-51 -0.7108579488231115 1107 52-53 0.7513159424164115 1107 54-55 1.1885236030505268 1107 56-57 1.2352078562588815 1107 58-59 1.4563583468186891 1107 60-61 1.6315715130146629 1107 62-63 1.4525722852903087 1107 64-65 1.4128565289140482 1107 66-67 1.3317711402651469 1107 68-69 1.1327812919465003 1107 70-71 1.6204117495370056 1107 72-73 1.1426047757331084 1107 74-75 1.0061245490004609 1107 76-77 1.1739935446963656 1107 78-79 1.1734739445563882 1107 80-81 1.3017424323094673 1107 82-83 1.3261902129917011 1107 84-85 1.1425398670513722 1107 86-87 1.1926140115411386 1107 88-89 1.3367122202719948 1107 90-91 1.5056839670221684 1107 92-93 1.50084847835209 1107 94-95 1.597405413740585 1107 96-97 1.7517611358384073 1107 98-99 1.5978441247878798 1107 100-101 1.536520771183202 1108 1 0.6264478992254858 1108 2 0.5700424794259895 1108 3 0.32106791389978895 1108 4 0.5698122937524488 1108 5 0.6586817418696072 1108 6 0.6286822387793407 1108 7 0.6584568373557929 1108 8 0.6827763035651699 1108 9 0.9594199091867637 1108 10-11 0.90023580241008 1108 12-13 0.8870384269200997 1108 14-15 1.1838033863081847 1108 16-17 0.9639653445317649 1108 18-19 0.7996216690558455 1108 20-21 0.7011789948838612 1108 22-23 0.7000691041060705 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1.0138483386557304 1108 92-93 0.9836006787305642 1108 94-95 1.1875241811569417 1108 96-97 1.1482164657776543 1108 98-99 0.8643131418410199 1108 100-101 0.9511574422628293 1109 1 0.2913459832400562 1109 2 0.28644786965478986 1109 3 0.1280993773737329 1109 4 0.5763677064175567 1109 5 -1.7797963996520991 1109 6 -0.09843492136074161 1109 7 -1.2549684723028491 1109 8 0.1308989236244784 1109 9 -1.3717700674243218 1109 10-11 0.27789557022563116 1109 12-13 0.6780106961457619 1109 14-15 0.9844605570271767 1109 16-17 -0.09572275319527535 1109 18-19 0.153549401584975 1109 20-21 0.585608511036753 1109 22-23 0.5602974906723617 1109 24-25 0.5206792459643523 1109 26-27 0.8071611553894158 1109 28-29 0.7374576642890105 1109 30-31 1.0787968179900602 1109 32-33 1.0762389544914654 1109 34-35 1.0198219658222385 1109 36-37 1.1207850764679108 1109 38-39 0.8021340254492841 1109 40-41 0.8577572115477281 1109 42-43 1.0034876141965583 1109 44-45 0.928355747503268 1109 46-47 1.0832755576077915 1109 48-49 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1.1676512528093994 2311 76-77 1.1710616591375356 2311 78-79 1.3148772304863208 2311 80-81 1.4216096775171891 2311 82-83 1.3131701955027637 2311 84-85 1.2963181177309089 2311 86-87 1.302769445079612 2311 88-89 1.494641368740762 2311 90-91 1.6007543611868336 2311 92-93 1.7350647108364932 2311 94-95 1.9216278313375845 2311 96-97 1.868118270401336 2311 98-99 2.016387613712734 2311 100-101 2.0794345516614676 2312 1 -0.03033552190026967 2312 2 -0.15275817849429885 2312 3 -0.3995878551528591 2312 4 0.12582365945992535 2312 5 -1.2334814773385574 2312 6 -0.12160720730674512 2312 7 0.09443584485823209 2312 8 -2.7357582551770747 2312 9 -4.913359759223798 2312 10-11 -0.48775005618784917 2312 12-13 -5.448794849146484 2312 14-15 -2.1045536355418193 2312 16-17 -2.6651223811614813 2312 18-19 -2.395562844420759 2312 20-21 -3.322765852751722 2312 22-23 -1.926736292513496 2312 24-25 -2.584823761415201 2312 26-27 -3.0776458988382984 2312 28-29 -5.867054134874778 2312 30-31 -6.432851589097744 2312 32-33 -1.7078281831017321 2312 34-35 0.3830621447414799 2312 36-37 0.6346633599306486 2312 38-39 0.45287658773847994 2312 40-41 -3.304695793870394 2312 42-43 -5.221569335801483 2312 44-45 -3.631102832947903 2312 46-47 -0.26446906737978537 2312 48-49 -1.3366323850583157 2312 50-51 -6.775523346893323 2312 52-53 -1.7277080552219815 2312 54-55 0.4988517897227851 2312 56-57 0.890345890719459 2312 58-59 0.9682220974905462 2312 60-61 1.351029814636405 2312 62-63 1.0842750358742492 2312 64-65 1.0849019994637388 2312 66-67 1.0429817083128228 2312 68-69 1.1345288279902945 2312 70-71 1.5307403738392367 2312 72-73 1.1073904822608363 2312 74-75 0.8992149287399442 2312 76-77 1.0727209963094104 2312 78-79 0.785340122085362 2312 80-81 0.9517407016818495 2312 82-83 -0.06453756167183045 2312 84-85 0.26725704868253075 2312 86-87 0.8465423969769716 2312 88-89 0.6794942499122705 2312 90-91 1.0141469349729313 2312 92-93 1.1425045258612023 2312 94-95 1.4754248118879758 2312 96-97 1.6618572194877963 2312 98-99 1.6422297499960017 2312 100-101 1.484086136192456 2313 1 -0.24451459100634665 2313 2 -0.37551625982902337 2313 3 -0.7458766029014612 2313 4 -1.1030842939005794 2313 5 -1.3233122669868607 2313 6 -0.9860611839870046 2313 7 -1.3398825739492395 2313 8 -5.571489130141529 2313 9 -2.103128931657203 2313 10-11 -1.7849523976420159 2313 12-13 -3.4574034850361386 2313 14-15 -3.071487705121868 2313 16-17 -7.163362735697461 2313 18-19 -3.766492553397917 2313 20-21 -4.942706131451121 2313 22-23 -3.868324357732952 2313 24-25 -0.8087667891903436 2313 26-27 -3.3738765842113168 2313 28-29 -5.015385730286667 2313 30-31 -2.959429937756383 2313 32-33 -6.057345909900484 2313 34-35 -2.3706526623492366 2313 36-37 -4.990579554089635 2313 38-39 -3.011444674940442 2313 40-41 -0.6993244316559597 2313 42-43 -1.635654794138766 2313 44-45 -4.931338637289542 2313 46-47 -2.7212659201620326 2313 48-49 -0.5402535813802487 2313 50-51 1.1160362796115564 2313 52-53 0.769720611002306 2313 54-55 -3.6982107280514143 2313 56-57 -5.984197904900981 2313 58-59 -0.653211213723786 2313 60-61 -8.892506302531096 2313 62-63 -2.7207242531578864 2313 64-65 -1.8720429716235678 2313 66-67 -2.9472839566791222 2313 68-69 -4.308694463316932 2313 70-71 -4.338157151992593 2313 72-73 -0.21532375605559295 2313 74-75 1.2419666113638925 2313 76-77 -2.2246116892532015 2313 78-79 0.6536688736257901 2313 80-81 1.5995035228023369 2313 82-83 1.7062108504999394 2313 84-85 1.9619378241114838 2313 86-87 1.9292516585183463 2313 88-89 -3.2129955595483395 2313 90-91 0.0979522719717032 2313 92-93 0.9386996628410067 2313 94-95 1.3313116258039877 2313 96-97 1.6039666611410333 2313 98-99 1.9542616961520345 2313 100-101 1.9467129254615791 2314 1 -28.73315095464271 2314 2 -14.913137619052325 2314 3 -5.113043416670216 2314 4 -4.32837114297384 2314 5 -1.581366106351382 2314 6 -1.2224910162906184 2314 7 -0.47437065868004424 2314 8 -3.8282190507064016 2314 9 -3.10941754595553 2314 10-11 -0.8483350101751128 2314 12-13 0.20350781240428262 2314 14-15 -1.6587008560486112 2314 16-17 -1.4370277842411525 2314 18-19 -3.3137674872019787 2314 20-21 -3.012653174699132 2314 22-23 -5.596203881122186 2314 24-25 -1.2126723496493028 2314 26-27 -3.8315663459059337 2314 28-29 -2.8527643710633654 2314 30-31 -4.922029231666357 2314 32-33 -2.4632925118422335 2314 34-35 -2.493832273999722 2314 36-37 -6.2123911163138175 2314 38-39 -7.956171065055184 2314 40-41 -7.708205720270264 2314 42-43 -5.711835288401783 2314 44-45 -3.710078707898546 2314 46-47 -5.872854622721611 2314 48-49 -5.457288003269035 2314 50-51 -3.0181759887026516 2314 52-53 -3.423902513446059 2314 54-55 -5.100847532994045 2314 56-57 -2.016358099075738 2314 58-59 -1.7851617874219343 2314 60-61 -0.6554696741109751 2314 62-63 0.5139955173628508 2314 64-65 -0.9741502090463428 2314 66-67 -0.35609904935343195 2314 68-69 0.18562371850124748 2314 70-71 0.5166530863127932 2314 72-73 0.28495206036100384 2314 74-75 -0.17390938157521063 2314 76-77 -1.0926059522717395 2314 78-79 -3.21482185894261 2314 80-81 -2.71272066078107 2314 82-83 -2.011463465475348 2314 84-85 -0.5218993338761599 2314 86-87 0.14637434166044727 2314 88-89 -0.21134066104878357 2314 90-91 0.32075677329561714 2314 92-93 0.13855623830437835 2314 94-95 0.5027591103582658 2314 96-97 0.3949529806467673 2314 98-99 1.2202590483144355 2314 100-101 1.4363422281976774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 19.0 4 45.0 5 123.0 6 257.0 7 727.0 8 1680.0 9 3135.0 10 4820.0 11 6966.0 12 9267.0 13 11743.0 14 14353.0 15 17461.0 16 19896.0 17 21713.0 18 20401.0 19 17506.0 20 16737.0 21 18123.0 22 19600.0 23 22433.0 24 26835.0 25 33517.0 26 41939.0 27 53532.0 28 67641.0 29 84911.0 30 105128.0 31 127689.0 32 155537.0 33 187620.0 34 228593.0 35 282194.0 36 354835.0 37 445305.0 38 414325.0 39 96673.0 40 73.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.61061456384674 21.681191058121797 15.441146606077547 23.267047771953912 2 14.444912622325676 22.186211161406142 40.74081649767945 22.62805971858873 3 17.71501080896614 26.786735228570173 31.579738668824938 23.91851529363875 4 12.249006936783498 18.667304390750775 39.78614197838102 29.297546694084716 5 11.827535070823462 38.83737024221453 36.65450764484292 12.680587042119079 6 28.96271099538481 39.924823359439934 18.330800400087544 12.781665245087712 7 25.199072189620225 33.66073967105277 23.60165380427483 17.538534335052184 8 24.07871797441703 37.18632366046013 21.055391125120085 17.679567240002754 9 24.761450869617686 16.52931554893743 21.689101705281114 37.02013187616377 10-11 23.112385191306345 27.588535050955254 29.914112085875644 19.38496767186276 12-13 24.011576493561304 25.13874883324845 29.744801009627874 21.104873663562373 14-15 21.198435376793423 26.89480793002422 27.444246491563597 24.46251020161876 16-17 20.67896247236103 29.89624859972768 28.123502143277427 21.30128678463386 18-19 20.54288975297253 28.872848114686306 30.152218038166495 20.43204409417466 20-21 21.375672522753785 28.001883847794918 30.20101876416039 20.421424865290906 22-23 21.061356984043545 27.995679354514518 29.909765531517124 21.033198129924816 24-25 21.00149355789152 28.088946563495643 29.99681717545546 20.91274270315737 26-27 21.04226447425236 28.191868470142733 29.848674454636686 20.91719260096822 28-29 20.899037939504005 28.250135388038228 29.948328390270746 20.902498282187025 30-31 21.238904414540873 28.004128431375058 29.87344533961973 20.88352181446434 32-33 21.136578486155198 28.13266724371101 29.694266499712445 21.036487770421353 34-35 21.301043302144098 28.142140819113294 29.772148075370303 20.78466780337231 36-37 21.220813342608313 28.00696953080269 29.718056842721406 21.054160283867585 38-39 21.10530356439127 28.11309807087328 29.799243049239298 20.98235531549615 40-41 21.367085759480727 27.979899908635947 29.699384997204533 20.953629334678794 42-43 21.21984745003467 28.03618680995754 29.507720566427142 21.23624517358064 44-45 21.356246647386065 28.19456487481614 29.34161693533885 21.107571542458945 46-47 21.36447675853951 28.084801084633316 29.191530803340648 21.359191353486526 48-49 21.323693121162744 28.228016896454495 29.110856302739624 21.33743367964314 50-51 21.3796323830611 28.300928809683327 28.972426767585002 21.347012039670567 52-53 21.433022741435995 28.32627762906518 28.858165685072635 21.38253394442619 54-55 21.427319340641727 28.23858511140781 28.91255676111648 21.42153878683398 56-57 21.490977471291956 28.2808343026546 28.76555743586457 21.46263079018888 58-59 21.449405945960873 28.313831533494994 28.847118230236806 21.389644290307327 60-61 21.437634982297823 28.40041182917953 28.744671022464807 21.417282166057845 62-63 21.420094761395333 28.32629679858124 28.70998226547782 21.543626174545608 64-65 21.324190233571127 28.28776898455815 28.705215835432384 21.682824946438338 66-67 21.301702020376787 28.29122418063198 28.699141770710085 21.70793202828115 68-69 21.39456156275169 28.32136297584195 28.63907816741455 21.64499729399181 70-71 21.467318811391063 28.070241530263857 28.59482335549973 21.867616302845345 72-73 21.39682661981929 28.11270439616898 28.67314784163305 21.81732114237868 74-75 21.37508870058144 28.252117436075498 28.51201035276198 21.860783510581086 76-77 21.276025633420808 28.2155468238108 28.459567499091488 22.048860043676903 78-79 21.332616066637623 28.290648465001546 28.357227450587345 22.019508017773486 80-81 21.307612535309506 28.362542298112803 28.332627654562405 21.99721751201529 82-83 21.422314555172584 28.177229719444913 28.471842133836606 21.928613591545897 84-85 21.478330370004464 28.198856838573832 28.30112875813083 22.02168403329087 86-87 21.23528154400477 28.28802099473393 28.359339816800627 22.11735764446067 88-89 21.355420880655206 28.124547747854635 28.335509257436282 22.184522114053877 90-91 21.337254720809952 28.090040092857382 28.33745636976316 22.235248816569513 92-93 21.38311369420731 28.05146563114025 28.33101821745339 22.23440245719905 94-95 21.209849001119206 28.02879863963621 28.30929867151121 22.452053687733372 96-97 21.147585245770863 28.015877453089345 28.386154768179097 22.450382532960695 98-99 21.08439300446596 28.010005795520403 28.308850100569327 22.59675109944431 100-101 21.102950113022693 27.942029015152254 28.27807144984225 22.676949421982805 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 231.0 1 168.5 2 129.5 3 172.0 4 158.0 5 151.5 6 276.5 7 556.0 8 925.0 9 1233.0 10 1262.5 11 1232.0 12 1403.0 13 1626.5 14 1872.0 15 2144.0 16 2455.5 17 2767.0 18 3008.0 19 3358.0 20 4041.0 21 4887.0 22 5970.5 23 7811.5 24 10397.5 25 13847.5 26 18692.5 27 25128.0 28 32569.5 29 40729.5 30 48729.5 31 56662.5 32 66398.0 33 75193.5 34 82059.5 35 89198.0 36 97554.0 37 104198.0 38 107538.0 39 109852.0 40 110857.5 41 112251.5 42 112998.0 43 112358.0 44 112179.5 45 112278.0 46 110711.5 47 108126.5 48 105336.5 49 101570.0 50 96922.0 51 91111.5 52 84582.5 53 78282.0 54 72870.5 55 65887.0 56 58059.5 57 50722.0 58 43848.5 59 37637.0 60 30817.0 61 24300.5 62 18953.5 63 14339.0 64 10462.5 65 7549.0 66 5457.5 67 4016.5 68 3032.5 69 2291.5 70 1755.5 71 1319.0 72 993.5 73 774.0 74 595.0 75 452.0 76 325.0 77 228.5 78 163.0 79 116.0 80 86.0 81 66.0 82 49.0 83 40.5 84 33.0 85 22.5 86 18.0 87 15.0 88 9.5 89 5.5 90 4.0 91 2.5 92 2.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11791946294997065 2 0.0 3 0.004943140250866072 4 3.409062241976602E-4 5 2.7272497935812814E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.014607831706869736 26-27 0.008335157181632791 28-29 0.004176101246421336 30-31 0.0013806702080005236 32-33 6.477218259755543E-4 34-35 9.374921165435654E-4 36-37 6.306765147656712E-4 38-39 3.409062241976602E-4 40-41 0.0012954436519511086 42-43 8.181749380743843E-4 44-45 4.261327802470752E-4 46-47 0.025704329304503577 48-49 0.014829420752598218 50-51 0.09107309779440491 52-53 0.0011590811622720445 54-55 0.03794286275319957 56-57 0.0011590811622720445 58-59 0.0013295342743708746 60-61 0.0035113341092358995 62-63 0.003272699752297537 64-65 0.009886280501732144 66-67 0.005710179255310807 68-69 0.002403388880593504 70-71 0.00560790738805151 72-73 0.004943140250866072 74-75 0.0011761264734819275 76-77 0.0 78-79 0.0 80-81 4.0908746903719215E-4 82-83 4.261327802470752E-4 84-85 5.113593362964902E-5 86-87 0.0017386217434080667 88-89 0.004142010624001571 90-91 0.0014488514528400557 92-93 0.0033920169307667183 94-95 8.522655604941504E-4 96-97 5.454499587162563E-4 98-99 0.001977256100346429 100-101 0.0033238356859271864 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2933358.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.31381516948728 #Duplication Level Percentage of deduplicated Percentage of total 1 83.18871658224387 58.493160419507575 2 10.918547088561953 15.354494038089772 3 2.8469722540307307 6.005444425877261 4 1.1122631524287057 3.128298628788131 5 0.5302986801646681 1.8643661690860762 6 0.32255398211595615 1.3608000648410066 7 0.21201276714544326 1.043519856584537 8 0.14470320235884462 0.8139707380074173 9 0.1048081264030332 0.6632513305346822 >10 0.5704291029539759 7.170549322629173 >50 0.03483773728375759 1.6761723372400024 >100 0.013222761398451062 1.580931858163449 >500 4.3931278442087767E-4 0.19889571907282338 >1k 9.762506315722283E-5 0.17960678127212187 >5k 9.762506315722283E-5 0.4665383103061251 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7540 0.25704329304503576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6053 0.2063505375068437 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3907 0.13319206179402582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.0227186725929805E-4 0.0 3.409062241976602E-5 0.0 5 0.0 1.0227186725929805E-4 0.0 3.409062241976602E-5 0.0 6 0.0 1.0227186725929805E-4 0.0 3.409062241976602E-5 0.0 7 0.0 1.0227186725929805E-4 0.0 3.409062241976602E-5 0.0 8 0.0 1.0227186725929805E-4 0.0 3.409062241976602E-5 0.0 9 0.0 1.7045311209883007E-4 0.0 3.409062241976602E-5 0.0 10-11 0.0 1.7045311209883007E-4 0.0 6.818124483953203E-5 0.0 12-13 0.0 1.7045311209883007E-4 0.0 1.0227186725929805E-4 0.0 14-15 0.0 1.7045311209883007E-4 0.0 1.0227186725929805E-4 0.0 16-17 0.0 1.7045311209883007E-4 0.0 1.0227186725929805E-4 0.0 18-19 0.0 1.874984233087131E-4 0.0 1.5340780088894706E-4 1.704531120988301E-5 20-21 0.0 2.045437345185961E-4 0.0 1.7045311209883007E-4 3.409062241976602E-5 22-23 0.0 2.045437345185961E-4 0.0 3.579515354075432E-4 3.409062241976602E-5 24-25 6.818124483953203E-5 2.2158904572847912E-4 0.0 6.306765147656713E-4 6.818124483953203E-5 26-27 6.818124483953203E-5 2.386343569383621E-4 0.0 0.0010397639838028634 6.818124483953203E-5 28-29 6.818124483953203E-5 2.386343569383621E-4 0.0 0.0027783857272109303 8.522655604941505E-5 30-31 6.818124483953203E-5 2.386343569383621E-4 0.0 0.008284021248003141 1.0227186725929805E-4 32-33 6.818124483953203E-5 2.386343569383621E-4 0.0 0.02261912797551475 1.0227186725929805E-4 34-35 6.818124483953203E-5 2.5567966814824515E-4 0.0 0.0464484730469312 1.0227186725929805E-4 36-37 6.818124483953203E-5 2.7272497935812814E-4 0.0 0.08543109978393364 1.0227186725929805E-4 38-39 6.818124483953203E-5 2.7272497935812814E-4 0.0 0.1382204286009413 1.0227186725929805E-4 40-41 6.818124483953203E-5 3.749968466174262E-4 0.0 0.1927142885389373 1.0227186725929805E-4 42-43 6.818124483953203E-5 3.9204215782730916E-4 0.0 0.2517933371923918 1.0227186725929805E-4 44-45 6.818124483953203E-5 4.602234026668412E-4 0.0 0.32466204261464165 1.0227186725929805E-4 46-47 6.818124483953203E-5 5.113593362964902E-4 0.0 0.411882900075613 1.0227186725929805E-4 48-49 6.818124483953203E-5 5.113593362964902E-4 0.0 0.5027173635130795 1.0227186725929805E-4 50-51 1.0227186725929805E-4 5.795405811360222E-4 0.0 0.5962279408104978 1.0227186725929805E-4 52-53 1.0227186725929805E-4 6.136312035557883E-4 0.0 0.6873010386049025 1.0227186725929805E-4 54-55 1.0227186725929805E-4 6.306765147656713E-4 0.0 0.7904251714246948 1.0227186725929805E-4 56-57 1.0227186725929805E-4 6.477218259755543E-4 0.0 0.9111400654130863 1.3636248967906407E-4 58-59 1.0227186725929805E-4 6.477218259755543E-4 0.0 1.0443321272071122 1.7045311209883007E-4 60-61 1.0227186725929805E-4 6.477218259755543E-4 0.0 1.177813959291706 1.7045311209883007E-4 62-63 1.0227186725929805E-4 6.477218259755543E-4 0.0 1.3097446680562004 1.7045311209883007E-4 64-65 1.0227186725929805E-4 7.840843156546183E-4 0.0 1.4524650588165509 1.7045311209883007E-4 66-67 1.0227186725929805E-4 8.181749380743844E-4 0.0 1.614446651244069 1.7045311209883007E-4 68-69 1.0227186725929805E-4 8.181749380743844E-4 0.0 1.7982803326426573 2.386343569383621E-4 70-71 1.0227186725929805E-4 8.181749380743844E-4 0.0 1.9932446022612993 2.7272497935812814E-4 72-73 1.0227186725929805E-4 8.181749380743844E-4 0.0 2.1967485727960923 2.897702905680111E-4 74-75 1.0227186725929805E-4 8.522655604941504E-4 0.0 2.4101558691438276 3.2386091298777716E-4 76-77 1.0227186725929805E-4 8.863561829139164E-4 0.0 2.639432350227964 3.4090622419766014E-4 78-79 1.0227186725929805E-4 9.034014941237995E-4 0.0 2.897634724435272 3.4090622419766014E-4 80-81 1.0227186725929805E-4 9.204468053336824E-4 0.0 3.172234688026487 3.749968466174262E-4 82-83 1.0227186725929805E-4 9.204468053336824E-4 0.0 3.4536357307904453 4.090874690371922E-4 84-85 1.0227186725929805E-4 9.204468053336824E-4 0.0 3.735957220359738 4.261327802470752E-4 86-87 1.0227186725929805E-4 9.886280501732144E-4 0.0 4.039346714584445 4.431780914569582E-4 88-89 1.0227186725929805E-4 0.0010227186725929804 0.0 4.368491674047286 4.431780914569582E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3975 0.0 50.48136 1 GTATCAA 7325 0.0 42.065216 1 ATCAACG 8690 0.0 34.75662 3 TCAACGC 8860 0.0 34.14275 4 CAACGCA 8915 0.0 33.82557 5 AACGCAG 9210 0.0 32.742126 6 TATCAAC 9490 0.0 32.427166 2 ACGCAGA 10565 0.0 28.093334 7 CGCAGAG 10590 0.0 27.488894 8 GCAGAGT 11645 0.0 24.345999 9 GAGTACT 7815 0.0 21.420168 12-13 GTGGTAT 2025 0.0 21.133587 1 GTACATG 6780 0.0 20.689407 1 AGAGTAC 10740 0.0 20.317675 10-11 CAGAGTA 11995 0.0 19.617163 10-11 GTACTTT 8615 0.0 19.403503 14-15 ACATGGG 6970 0.0 18.941404 3 TACATGG 7140 0.0 18.756468 2 TGGTATC 2110 0.0 18.455746 2 ACGACCG 130 0.007575991 18.264988 5 >>END_MODULE