FastQCFastQC Report
Sat 18 Jun 2016
SRR1931144_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931144_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118364
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70550.22624042606956724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52500.16835751054078354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.10200861733909192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37750.054.871191
GTATCAA74050.039.9797131
ATCAACG84800.033.4401973
CAACGCA86700.032.9820335
TCAACGC87800.032.9047284
AACGCAG90700.031.822776
TATCAAC92300.031.3983422
ACGCAGA102100.027.4427597
CGCAGAG102450.026.9771068
GTACATG76650.023.951641
GCAGAGT112500.023.0872029
TACATGG80000.021.567292
GAGTACT71550.021.37035612-13
ACATGGG77350.021.0461313
CAGAGTA112700.020.21740210-11
AGAGTAC103900.020.05267310-11
GTGGTAT19400.019.5708371
GTACTTT85900.018.31335414-15
GGCGTCG4550.017.7733278
AGTACTT81500.017.76928912-13
Produced by FastQC (version 0.11.5)