##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931144_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3118364 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.45348426290196 31.0 28.0 31.0 22.0 34.0 2 28.120778395338068 31.0 28.0 33.0 16.0 34.0 3 28.21378357369441 31.0 27.0 33.0 16.0 34.0 4 31.35729857066077 35.0 31.0 37.0 17.0 37.0 5 31.45781121126334 35.0 32.0 37.0 17.0 37.0 6 31.74964917501613 35.0 32.0 37.0 18.0 37.0 7 31.37850776881724 35.0 31.0 37.0 17.0 37.0 8 31.58108867341978 35.0 32.0 37.0 17.0 37.0 9 32.54654363634265 37.0 31.0 39.0 17.0 39.0 10-11 32.55869407804862 37.0 31.5 39.0 17.0 39.0 12-13 32.60135795564597 37.0 32.0 39.0 17.0 39.0 14-15 33.31326121645837 38.0 31.0 40.0 17.0 41.0 16-17 33.30260546876503 38.0 31.0 40.0 17.0 41.0 18-19 33.34665356578 38.0 31.5 40.0 17.5 41.0 20-21 33.63453272292779 38.0 32.0 40.0 19.0 41.0 22-23 33.50791841491244 38.0 32.0 40.0 18.0 41.0 24-25 33.39877608900051 38.0 31.5 40.0 18.5 41.0 26-27 33.04354366584529 37.0 31.0 40.0 16.5 41.0 28-29 32.99286629142717 37.0 31.0 40.0 17.0 41.0 30-31 32.71736509913532 37.0 30.5 40.0 15.5 41.0 32-33 32.52145836727207 37.0 30.0 40.0 15.5 41.0 34-35 32.502732843247294 37.0 30.0 40.0 16.0 41.0 36-37 32.12092783267123 36.0 30.0 40.0 9.5 41.0 38-39 31.950230954436364 36.0 30.0 40.0 10.0 41.0 40-41 31.80939139882323 36.5 30.0 40.0 9.0 41.0 42-43 32.03339411306698 37.0 30.0 40.0 9.0 41.0 44-45 32.08240522915221 37.0 30.0 40.0 8.5 41.0 46-47 31.860699713054665 37.0 30.0 40.0 8.0 41.0 48-49 31.650844000251414 36.0 30.0 40.0 7.0 41.0 50-51 31.1301278490901 35.5 29.5 39.5 3.5 40.5 52-53 31.089167428818445 36.0 29.0 39.5 2.0 40.5 54-55 31.090878903168456 36.0 29.0 40.0 2.0 41.0 56-57 30.843960166292327 35.5 29.0 40.0 2.0 41.0 58-59 30.704884997389655 35.0 28.5 40.0 2.0 41.0 60-61 30.57158817892972 35.0 28.0 39.0 2.0 41.0 62-63 30.424971715938227 35.0 28.5 39.0 2.0 41.0 64-65 30.090780774790886 35.0 28.0 39.0 2.0 40.0 66-67 29.871739155531557 34.0 28.0 39.0 2.0 40.0 68-69 29.687135946926016 34.0 28.0 38.5 2.0 40.0 70-71 29.401061582291227 34.0 28.0 38.0 2.0 40.0 72-73 29.080970502481428 34.0 28.0 37.0 2.0 40.0 74-75 28.666734383798683 34.0 27.0 37.0 2.0 39.5 76-77 28.36283192084054 34.0 27.0 36.0 2.0 39.0 78-79 27.959670679882144 33.0 26.0 36.0 2.0 39.0 80-81 27.57050572030719 33.0 26.0 35.5 2.0 37.5 82-83 27.23412067994628 33.0 26.0 35.0 2.0 37.0 84-85 26.899275870296094 33.0 26.0 35.0 2.0 37.0 86-87 26.585900812092497 32.5 25.0 35.0 2.0 36.0 88-89 26.23345222045919 32.0 25.0 35.0 2.0 36.0 90-91 25.840426261975832 32.0 23.5 35.0 2.0 36.0 92-93 25.47727237743894 32.0 21.0 35.0 2.0 35.0 94-95 25.18247019911723 32.0 20.0 35.0 2.0 35.0 96-97 24.788816347289796 32.0 17.5 35.0 2.0 35.0 98-99 24.43781402684228 32.0 4.5 34.0 2.0 35.0 100-101 23.853246926914238 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.211388275868767 1101 2 5.931406519589654 1101 3 5.528878433197917 1101 4 6.906747904571677 1101 5 6.348755356351461 1101 6 5.7996265589796785 1101 7 6.284004524893813 1101 8 5.788994140084213 1101 9 6.415818212333754 1101 10-11 6.387789928199201 1101 12-13 6.3220483951632644 1101 14-15 6.584499018119896 1101 16-17 6.62764909355846 1101 18-19 6.604612573614542 1101 20-21 5.917367503417626 1101 22-23 6.063555689224401 1101 24-25 6.03247278046366 1101 26-27 6.220645809948746 1101 28-29 6.017161961833189 1101 30-31 6.159417462662777 1101 32-33 5.981675395174587 1101 34-35 5.803672023634178 1101 36-37 5.894369652194555 1101 38-39 5.880519828838349 1101 40-41 6.160441494007596 1101 42-43 5.961490416894122 1101 44-45 5.897502365079273 1101 46-47 5.670372413378594 1101 48-49 5.731104191794252 1101 50-51 5.7131898268686605 1101 52-53 5.477545288996325 1101 54-55 5.593764314263989 1101 56-57 5.4508327413642235 1101 58-59 5.271426117095519 1101 60-61 5.185401722229969 1101 62-63 5.1686014524595905 1101 64-65 5.162693367045776 1101 66-67 5.104035898431274 1101 68-69 5.081759215829944 1101 70-71 5.073204559000306 1101 72-73 4.790721433071827 1101 74-75 4.943905336087614 1101 76-77 4.71866825267054 1101 78-79 4.766709719533129 1101 80-81 4.675401785460561 1101 82-83 4.554895982111557 1101 84-85 4.449460670772371 1101 86-87 4.248905186052493 1101 88-89 4.436657820264603 1101 90-91 4.366415704394456 1101 92-93 4.138652574584228 1101 94-95 4.1757696096657035 1101 96-97 4.171690143659941 1101 98-99 3.8195641224772174 1101 100-101 3.9207442238595362 1102 1 5.5514112402201725 1102 2 6.116304586560492 1102 3 5.824364887002048 1102 4 7.082370553407369 1102 5 6.681779233848879 1102 6 6.2130824597998675 1102 7 6.662246720970483 1102 8 6.142386386341556 1102 9 6.953942094325825 1102 10-11 6.969171751179832 1102 12-13 7.04037024129493 1102 14-15 7.261601418707382 1102 16-17 7.166486447161077 1102 18-19 7.177352609028926 1102 20-21 6.453389957705401 1102 22-23 6.566633518300534 1102 24-25 6.501982549287948 1102 26-27 6.586491822714411 1102 28-29 6.423036230573253 1102 30-31 6.66027123049221 1102 32-33 6.407365930435535 1102 34-35 6.519540660421626 1102 36-37 6.553139415185846 1102 38-39 6.471500885689618 1102 40-41 6.709350629876223 1102 42-43 6.535533038799258 1102 44-45 6.4733452516947985 1102 46-47 6.400587810436278 1102 48-49 6.208656509575384 1102 50-51 6.124544048686314 1102 52-53 6.016414449776505 1102 54-55 6.314886879309679 1102 56-57 6.112008469630126 1102 58-59 6.217472240805765 1102 60-61 6.133463186046534 1102 62-63 6.1015340799751385 1102 64-65 5.973145564591462 1102 66-67 6.1088936370601346 1102 68-69 6.055496287791851 1102 70-71 5.922253115133941 1102 72-73 5.825060691730524 1102 74-75 5.96434381705723 1102 76-77 5.811113588481817 1102 78-79 5.734787914068701 1102 80-81 5.625643153832623 1102 82-83 5.513290327068223 1102 84-85 5.426163887691434 1102 86-87 5.466261580235756 1102 88-89 5.570744746767911 1102 90-91 5.592129955029559 1102 92-93 5.582406068527295 1102 94-95 5.4732971795959 1102 96-97 5.417475656732687 1102 98-99 5.105767798697666 1102 100-101 5.152926639961464 1103 1 5.152187441237498 1103 2 5.745050082193988 1103 3 5.3817308544315665 1103 4 6.761933367446712 1103 5 6.3884316700200685 1103 6 5.787345227391132 1103 7 6.30445548882491 1103 8 5.708397623707334 1103 9 6.368430266422223 1103 10-11 6.474581546832656 1103 12-13 6.55678709943858 1103 14-15 6.781470530386695 1103 16-17 6.629575537514732 1103 18-19 6.607751366625177 1103 20-21 5.945057211490013 1103 22-23 6.106227066708925 1103 24-25 5.912193070891181 1103 26-27 6.166871546209844 1103 28-29 5.9609553090271845 1103 30-31 6.179281691140407 1103 32-33 6.032975498769677 1103 34-35 5.982581179021594 1103 36-37 5.847314887067256 1103 38-39 5.706547422021369 1103 40-41 5.932251393837451 1103 42-43 5.909626156805942 1103 44-45 5.6921208603806654 1103 46-47 5.6597389225429 1103 48-49 5.4468254491657575 1103 50-51 5.2819304133825575 1103 52-53 5.065250191297864 1103 54-55 5.373896466096777 1103 56-57 5.5098178763645365 1103 58-59 5.558929911419245 1103 60-61 5.298413758368163 1103 62-63 5.398831632260087 1103 64-65 5.208652241162849 1103 66-67 5.371408432791156 1103 68-69 5.294616896550181 1103 70-71 5.241841945553379 1103 72-73 5.038274332400849 1103 74-75 5.175885032521961 1103 76-77 5.12799506378893 1103 78-79 5.145892277907151 1103 80-81 4.963486282421783 1103 82-83 4.876045778978533 1103 84-85 4.8842482937765475 1103 86-87 4.8404659397551235 1103 88-89 4.808770518065121 1103 90-91 4.698466976133229 1103 92-93 4.720083242428952 1103 94-95 4.699919924578698 1103 96-97 4.590971700896969 1103 98-99 4.42146141840681 1103 100-101 4.467425659988663 1104 1 4.742713070262376 1104 2 5.513960817296976 1104 3 5.171347652687693 1104 4 6.566982472405424 1104 5 6.167323141877425 1104 6 5.81918946080626 1104 7 6.322090749990846 1104 8 5.760726616355704 1104 9 6.52736576935569 1104 10-11 6.419591794744871 1104 12-13 6.343809564447696 1104 14-15 6.390392853131001 1104 16-17 6.361815897943181 1104 18-19 6.530325388934568 1104 20-21 5.883285789865855 1104 22-23 6.037758890422694 1104 24-25 5.79812042465797 1104 26-27 5.9217802212254576 1104 28-29 5.8017448919995545 1104 30-31 6.04061268995083 1104 32-33 5.959061355166124 1104 34-35 5.796436219071303 1104 36-37 5.806690280564531 1104 38-39 5.659283277724487 1104 40-41 6.026181006304871 1104 42-43 6.014401552236137 1104 44-45 5.94895351385809 1104 46-47 5.944180745734236 1104 48-49 5.735545476316105 1104 50-51 5.636842127825421 1104 52-53 5.5330587348337446 1104 54-55 5.705446225232674 1104 56-57 5.70932738704056 1104 58-59 5.6793806276665855 1104 60-61 5.411160962200135 1104 62-63 5.411432369314195 1104 64-65 5.509629143896834 1104 66-67 5.419545256881015 1104 68-69 5.432803616270025 1104 70-71 5.388968678324552 1104 72-73 5.347541167921474 1104 74-75 5.389119553188177 1104 76-77 5.331638405629391 1104 78-79 5.280559599988255 1104 80-81 5.305275024579327 1104 82-83 4.979632681316129 1104 84-85 4.904102371854531 1104 86-87 4.92337846882052 1104 88-89 4.946303900991072 1104 90-91 5.02541434908062 1104 92-93 4.9271638333328305 1104 94-95 4.785476020009991 1104 96-97 4.726135451090784 1104 98-99 4.593818128670815 1104 100-101 4.666875012415581 1105 1 4.5273237007986715 1105 2 5.236660650757841 1105 3 4.863127027343747 1105 4 6.269306002034686 1105 5 3.782467384810687 1105 6 5.093669770708811 1105 7 5.724810515302199 1105 8 1.3020326511081528 1105 9 0.7447539740508411 1105 10-11 2.3513090871872784 1105 12-13 5.578125675054679 1105 14-15 3.722918570394693 1105 16-17 5.831130918812711 1105 18-19 6.226636563276028 1105 20-21 3.434966307927784 1105 22-23 5.4175576300829675 1105 24-25 5.561789317635107 1105 26-27 5.820480961706831 1105 28-29 3.1966047753615072 1105 30-31 5.470271674834013 1105 32-33 5.712412474388383 1105 34-35 5.589406897364938 1105 36-37 5.766289604503548 1105 38-39 5.810413638324963 1105 40-41 5.886027060429132 1105 42-43 3.16144018344945 1105 44-45 5.192693817666747 1105 46-47 5.511043990290183 1105 48-49 5.435973307321461 1105 50-51 5.507709818908996 1105 52-53 5.334229074631573 1105 54-55 5.5291876117160506 1105 56-57 5.439528855938505 1105 58-59 5.370793529808996 1105 60-61 5.236696385563757 1105 62-63 5.107978483238995 1105 64-65 5.100167713498237 1105 66-67 5.242081326442744 1105 68-69 5.167527412488209 1105 70-71 4.985626790503776 1105 72-73 5.064178999268339 1105 74-75 5.199731909190568 1105 76-77 5.064255327644155 1105 78-79 5.0280199882605 1105 80-81 4.980197972289631 1105 82-83 4.806917563280074 1105 84-85 4.880179809769206 1105 86-87 4.903611449950819 1105 88-89 4.896249804645986 1105 90-91 4.884586452732556 1105 92-93 4.911771260714062 1105 94-95 4.944745281877598 1105 96-97 4.941575028244404 1105 98-99 4.898464577211069 1105 100-101 4.957005144432927 1106 1 4.288226034195333 1106 2 4.60764890360981 1106 3 4.747081935814602 1106 4 6.236873822726505 1106 5 6.1647450962270725 1106 6 5.416132349990615 1106 7 5.969713102500098 1106 8 5.594513485476966 1106 9 6.149586751173288 1106 10-11 6.096654859605476 1106 12-13 6.346145332863195 1106 14-15 6.051426840714381 1106 16-17 6.094889874492438 1106 18-19 6.194777393679427 1106 20-21 5.443613553765321 1106 22-23 5.574118207954129 1106 24-25 5.694270571936311 1106 26-27 5.831664654406207 1106 28-29 5.720686677463668 1106 30-31 5.81600642804224 1106 32-33 5.614447912445552 1106 34-35 5.2711607477351095 1106 36-37 5.380263651208686 1106 38-39 5.448692975505903 1106 40-41 5.5378076151837625 1106 42-43 2.5655034298712103 1106 44-45 4.724143369006757 1106 46-47 4.961031908025063 1106 48-49 4.848673140112865 1106 50-51 4.948701191784917 1106 52-53 4.775793848603428 1106 54-55 5.115354905411149 1106 56-57 5.03180952714149 1106 58-59 4.9240366510448546 1106 60-61 4.718337927043454 1106 62-63 4.780648072610504 1106 64-65 4.785778817799773 1106 66-67 4.864130921562538 1106 68-69 4.774239391639668 1106 70-71 4.742863671323374 1106 72-73 4.559019126128437 1106 74-75 4.648765309562666 1106 76-77 4.589580588759681 1106 78-79 4.801707029598333 1106 80-81 4.64235058069135 1106 82-83 4.665643813502125 1106 84-85 4.6254332845485315 1106 86-87 4.783018149953612 1106 88-89 4.843532634909224 1106 90-91 4.904941155620726 1106 92-93 4.861437161219005 1106 94-95 4.82033888531279 1106 96-97 4.884728085955363 1106 98-99 4.747570577077905 1106 100-101 4.786357124299016 1107 1 2.7953180401263715 1107 2 -0.3574465964604343 1107 3 0.6694200930882666 1107 4 2.466081698235289 1107 5 2.559796345960489 1107 6 3.9925714461436286 1107 7 4.806944661743298 1107 8 4.216786374009175 1107 9 4.750002949950087 1107 10-11 2.692119869697745 1107 12-13 4.811840961702124 1107 14-15 3.1508145451245575 1107 16-17 1.6509916174180006 1107 18-19 2.1949790369457034 1107 20-21 1.92420279935671 1107 22-23 4.103294320858197 1107 24-25 4.059593274441237 1107 26-27 2.994449582911283 1107 28-29 3.7667873692163027 1107 30-31 3.0721156757359758 1107 32-33 3.788374578984186 1107 34-35 4.327905576014892 1107 36-37 4.444953552671329 1107 38-39 4.515905628797736 1107 40-41 3.064626295941533 1107 42-43 3.637724442009226 1107 44-45 3.9665643112013385 1107 46-47 4.3089453803221005 1107 48-49 1.9819901667894086 1107 50-51 3.5248993520301646 1107 52-53 3.772667935775985 1107 54-55 4.315427413600698 1107 56-57 2.9521011189710435 1107 58-59 2.02175637650679 1107 60-61 3.0822297962209397 1107 62-63 3.953887812809075 1107 64-65 4.0887496573533895 1107 66-67 4.174593515978586 1107 68-69 4.03858872318466 1107 70-71 3.92057790844979 1107 72-73 3.900071926341905 1107 74-75 4.071509280226859 1107 76-77 3.9459052763642255 1107 78-79 3.9641480527332327 1107 80-81 4.039402154367963 1107 82-83 3.799102344225183 1107 84-85 3.7386794783423696 1107 86-87 3.789952164089865 1107 88-89 3.8927956262441192 1107 90-91 3.9226962495137165 1107 92-93 3.996808081227776 1107 94-95 4.09261073783323 1107 96-97 4.064123947063074 1107 98-99 3.976100938792573 1107 100-101 4.120780586276371 1108 1 -2.434865240991442 1108 2 -1.65347314270603 1108 3 -2.362001021193997 1108 4 -4.46371322028061 1108 5 -9.231691168797756 1108 6 -7.394221204018596 1108 7 -6.146658673852194 1108 8 -6.4713225488017265 1108 9 -8.1964408943516 1108 10-11 -3.743680317627362 1108 12-13 -2.508110208266359 1108 14-15 -7.1001662389449045 1108 16-17 -6.490869676008423 1108 18-19 -4.930675787489303 1108 20-21 -5.190497224814273 1108 22-23 -4.022784657824861 1108 24-25 -8.782270401221375 1108 26-27 -9.76298502761053 1108 28-29 -6.948540926171688 1108 30-31 -6.782403193898762 1108 32-33 -3.203562713586951 1108 34-35 -1.6610594411372048 1108 36-37 -5.2262554380042445 1108 38-39 -5.979756958241037 1108 40-41 -3.932519087865767 1108 42-43 -4.8079867194064505 1108 44-45 -2.35932095100733 1108 46-47 -3.002260368188125 1108 48-49 -1.933617663679044 1108 50-51 -1.2246175188503123 1108 52-53 -2.8008868022012763 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-5.441039671865877 2313 52-53 -4.072797186000617 2313 54-55 -7.4254688905503805 2313 56-57 -5.306040116220572 2313 58-59 -4.684536934222788 2313 60-61 -2.990315253691932 2313 62-63 -5.112787071357943 2313 64-65 -5.152043646622083 2313 66-67 -6.995097584806242 2313 68-69 -5.983736142746256 2313 70-71 -6.760424637849631 2313 72-73 -6.690659408675451 2313 74-75 -7.5190515135161355 2313 76-77 -6.866964511151476 2313 78-79 -7.942183418717143 2313 80-81 -9.41481426757479 2313 82-83 -8.14768540111786 2313 84-85 -8.217145266014573 2313 86-87 -7.206045181544951 2313 88-89 -8.205782295921516 2313 90-91 -8.105382273767702 2313 92-93 -7.28171705756918 2313 94-95 -7.637699343351171 2313 96-97 -7.876739599655103 2313 98-99 -7.915788557215748 2313 100-101 -7.878724675175224 2314 1 -13.11763506886327 2314 2 -14.5590363457731 2314 3 -13.90123472739282 2314 4 -24.913808277314832 2314 5 -18.679079466921856 2314 6 -17.375580559050704 2314 7 -16.467902146076607 2314 8 -12.600993556800717 2314 9 -14.742435088378391 2314 10-11 -13.848962392473933 2314 12-13 -14.85158262989361 2314 14-15 -16.62822285507226 2314 16-17 -17.405319824313175 2314 18-19 -15.55477747879454 2314 20-21 -16.225196986580045 2314 22-23 -16.678115148650477 2314 24-25 -17.522968814045 2314 26-27 -15.780201296168347 2314 28-29 -17.741083386295067 2314 30-31 -17.792295929332557 2314 32-33 -14.823232518581982 2314 34-35 -16.023912059347136 2314 36-37 -15.583738911490826 2314 38-39 -14.583760594862845 2314 40-41 -17.427788915737594 2314 42-43 -17.811020853164713 2314 44-45 -14.922224253154596 2314 46-47 -14.370900717335374 2314 48-49 -13.883033319296484 2314 50-51 -13.505607196681908 2314 52-53 -13.019829578655532 2314 54-55 -12.956956018309945 2314 56-57 -11.821663325225405 2314 58-59 -11.103475060354697 2314 60-61 -11.75141655810953 2314 62-63 -14.857714973056662 2314 64-65 -12.890472385073746 2314 66-67 -13.607795597231451 2314 68-69 -16.458301145841098 2314 70-71 -16.19628122068817 2314 72-73 -14.593630454731251 2314 74-75 -13.024713923823096 2314 76-77 -13.320255357303559 2314 78-79 -13.015095548196065 2314 80-81 -13.34226893390523 2314 82-83 -13.443070651341376 2314 84-85 -13.907271581321421 2314 86-87 -14.529341300386793 2314 88-89 -13.806903315613866 2314 90-91 -13.508353319823502 2314 92-93 -13.58579078352686 2314 94-95 -13.28219396750009 2314 96-97 -13.132717225110731 2314 98-99 -13.228252737312491 2314 100-101 -12.77147128804517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 113601.0 3 17939.0 4 12360.0 5 9740.0 6 10309.0 7 12296.0 8 14100.0 9 16282.0 10 17525.0 11 19056.0 12 20680.0 13 22574.0 14 24577.0 15 28018.0 16 30106.0 17 31452.0 18 29873.0 19 26675.0 20 27013.0 21 28997.0 22 33734.0 23 41196.0 24 50662.0 25 61583.0 26 72462.0 27 82046.0 28 91041.0 29 100604.0 30 112025.0 31 124080.0 32 141406.0 33 167200.0 34 201741.0 35 242727.0 36 297305.0 37 359116.0 38 333762.0 39 92353.0 40 148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.79319253300068 20.504986919083123 14.492513685712764 23.209306862203434 2 14.385581077684437 20.78884334981226 41.514170349171934 23.31140522333137 3 17.583130128490453 26.360040072294318 30.93115492610869 25.12567487310654 4 11.274891055010727 17.837493979759085 40.56698814403674 30.320626821193443 5 12.142782994415267 37.904284049044854 36.12467094046348 13.828262016076392 6 28.903480770397415 38.72221853091916 18.987292608952018 13.387008089731406 7 25.405349704406426 32.773712973758684 23.704434674076623 18.116502647758264 8 24.23470796335176 36.020442886402016 21.599893419624912 18.144955730621312 9 24.948668989135417 16.43661342451998 22.03512851692864 36.57958906941596 10-11 23.120631111305247 27.006669681502316 29.942858372528335 19.929840834664102 12-13 24.205329784021963 24.69687919952166 29.557883519018173 21.539907497438207 14-15 21.52821483709711 26.3326491660266 27.360730423524394 24.778405573351897 16-17 20.7477295082612 29.33413839311587 28.139648867856586 21.77848323076634 18-19 20.549579192299724 28.410935058636877 30.166171268171972 20.873314480891427 20-21 21.519766792302605 27.7104937042911 30.13638027237529 20.633359231031008 22-23 21.30433379897078 27.513025730120646 29.8913721281076 21.291268342800972 24-25 21.107449532296744 27.679224724660706 29.917039106100418 21.29628663694213 26-27 20.960805407922415 27.84916263909183 29.956164999438013 21.233866953547746 28-29 20.900878544543524 27.830020820348373 29.991060942095483 21.278039693012623 30-31 21.10672618295527 27.68515662841713 29.93191632420397 21.276200864423625 32-33 21.03595539449689 27.86693143118899 29.761082761062536 21.33603041325158 34-35 21.30918750385398 27.878730653018437 29.839603759429405 20.972478083698178 36-37 21.171646332162876 27.599902381537266 29.800411988446342 21.42803929785352 38-39 21.107390653216505 27.896787476656158 29.894403643565575 21.10141822656176 40-41 21.179486833775695 27.636904463816798 29.826406411110813 21.35720229129669 42-43 21.15972418734574 27.818738843604773 29.624435669582745 21.39710129946674 44-45 21.210991929480937 27.993752353219296 29.466398195038668 21.328857522261092 46-47 21.245899766122772 27.945592450193363 29.279621867721428 21.528885915962437 48-49 21.227087050110775 28.13907333235325 29.272065861670722 21.36177375586526 50-51 21.15924390912801 28.17635787013211 29.055781849191032 21.608616371548848 52-53 21.15604235047308 28.37517645495496 28.935836195878323 21.53294499869364 54-55 21.149520338975147 28.22326403011111 28.912774240487792 21.71444139042595 56-57 21.158999039703513 28.30035564749237 28.782578902752686 21.758066410051423 58-59 21.154196981947734 28.37423436252727 28.936760926773253 21.53480772875174 60-61 21.26771513306414 28.436940975706822 28.743181298800074 21.552162592428964 62-63 21.214601630855153 28.331618759067258 28.817931453800778 21.635848156276815 64-65 21.11198371966839 28.26797962008284 28.847482205412838 21.772554454835934 66-67 21.140845007189668 28.235173956600317 28.76845037975041 21.855530656459603 68-69 21.1707966100173 28.21291869711169 28.760978513092123 21.855306179778882 70-71 21.212292727853452 28.076597215719524 28.69708282932974 22.014027227097284 72-73 21.32565345161758 28.155388530652615 28.689081839066894 21.829876178662914 74-75 21.16924130730088 28.370950280339308 28.68217116411041 21.777637248249402 76-77 21.294483261094598 28.2510316306884 28.5735084165928 21.8809766916242 78-79 21.284189401878677 28.251560754292953 28.548495300741028 21.91575454308734 80-81 21.297321287700857 28.349480689233204 28.49430021639552 21.85889780667042 82-83 21.433370190266434 28.186526653078346 28.61883667204983 21.76126648460539 84-85 21.404108051529583 28.27192399604408 28.4149958119065 21.908972140519836 86-87 21.26058728230572 28.312041185698654 28.5711995135911 21.85617201840452 88-89 21.403995813189226 28.127296877465234 28.48407049337409 21.984636815971452 90-91 21.466416364478295 28.089296182228885 28.540574480721304 21.903712972571515 92-93 21.486907878618403 28.129878359293524 28.52333787845165 21.85987588363642 94-95 21.264804237093553 28.018473789461396 28.570173334479232 22.146548638965815 96-97 21.31919173002254 27.977057841868362 28.61789066318108 22.08585976492802 98-99 21.308962007001107 27.975342198665707 28.438597931479453 22.277097862853726 100-101 21.39605895912087 27.867112370460923 28.43450924908061 22.3023194213376 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 396.0 1 272.0 2 195.5 3 251.0 4 224.5 5 248.0 6 408.5 7 746.5 8 1271.0 9 1688.0 10 1720.5 11 1650.5 12 1780.5 13 2069.0 14 2356.5 15 2586.5 16 2910.0 17 3270.0 18 3639.5 19 4105.0 20 4777.0 21 5761.5 22 7201.0 23 9381.5 24 12223.0 25 16038.0 26 21305.5 27 27522.0 28 34611.5 29 43166.5 30 52044.5 31 60210.0 32 69649.5 33 79131.0 34 87134.0 35 94471.0 36 102767.5 37 110523.5 38 114383.5 39 115704.5 40 116469.5 41 116481.5 42 115361.5 43 114841.5 44 114629.5 45 114704.0 46 113138.0 47 110021.5 48 107957.0 49 104976.5 50 100617.5 51 95907.0 52 90548.5 53 84569.0 54 78279.5 55 70907.5 56 63509.0 57 56072.5 58 48689.5 59 42283.5 60 35487.5 61 28690.0 62 22657.5 63 17489.0 64 13332.0 65 9916.5 66 7273.5 67 5568.5 68 4413.5 69 3430.5 70 2596.5 71 1954.0 72 1479.0 73 1119.5 74 846.0 75 645.5 76 472.0 77 329.0 78 265.0 79 201.5 80 144.5 81 107.0 82 75.0 83 62.5 84 57.5 85 45.5 86 34.5 87 25.0 88 17.0 89 13.5 90 16.0 91 14.5 92 7.0 93 4.0 94 3.5 95 3.0 96 1.5 97 2.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05162963656584029 2 0.740708910184956 3 0.0 4 0.5902133298101184 5 0.2195061256479359 6 0.09405572922211776 7 0.10505508657744894 8 0.10723571719016767 9 0.10543990374439931 10-11 0.15623576978184714 12-13 0.2172453247921025 14-15 0.1319922882639743 16-17 0.1249212728212614 18-19 0.1510086699307714 20-21 0.11364933663934038 22-23 0.10516732491780947 24-25 0.09557896384129627 26-27 0.14154858124324166 28-29 0.10847033893413341 30-31 0.12937873833843644 32-33 0.1007900296437491 34-35 0.15072005705555863 36-37 0.134653940335381 38-39 0.12993993004023907 40-41 0.1049268141884655 42-43 0.1051352568205636 44-45 0.09463295497254329 46-47 0.09108943022687538 48-49 0.09575533837614851 50-51 0.08255931635947568 52-53 0.09100925998376072 54-55 0.2996924028112177 56-57 0.3190454994990963 58-59 0.3313596488415079 60-61 0.15904172829086022 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3118364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.11084772799893 #Duplication Level Percentage of deduplicated Percentage of total 1 83.3843240616444 57.62761323126424 2 10.416839910732918 14.398332737552098 3 2.888852567558556 5.989531497155343 4 1.1491856102256404 3.1768476687804714 5 0.6205321471220688 2.144275136504077 6 0.347191364447112 1.4396813712468326 7 0.22718882276879523 1.0990848495114283 8 0.1626971734969794 0.8995311666660455 9 0.11961049312415996 0.7439744319277214 >10 0.6224969293622905 7.829425478074628 >50 0.044747292927525086 2.0922669098352316 >100 0.015763784561927004 1.8359504838847902 >500 3.798946851914828E-4 0.17856575598006924 >1k 9.497367125841474E-5 0.14108696649428806 >5k 9.497367125841474E-5 0.40383231512263656 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7055 0.22624042606956724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5250 0.16835751054078354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3181 0.10200861733909192 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 6.413619449172707E-5 0.0 0.0 0.0 6 3.2068097245863535E-5 6.413619449172707E-5 0.0 0.0 0.0 7 3.2068097245863535E-5 6.413619449172707E-5 0.0 0.0 0.0 8 3.2068097245863535E-5 6.413619449172707E-5 0.0 0.0 0.0 9 3.2068097245863535E-5 6.413619449172707E-5 0.0 0.0 0.0 10-11 3.2068097245863535E-5 6.413619449172707E-5 0.0 1.6034048622931767E-5 0.0 12-13 3.2068097245863535E-5 6.413619449172707E-5 0.0 3.2068097245863535E-5 0.0 14-15 3.2068097245863535E-5 6.413619449172707E-5 0.0 3.2068097245863535E-5 0.0 16-17 3.2068097245863535E-5 6.413619449172707E-5 0.0 3.2068097245863535E-5 0.0 18-19 3.2068097245863535E-5 6.413619449172707E-5 0.0 3.2068097245863535E-5 6.413619449172707E-5 20-21 3.2068097245863535E-5 9.62042917375906E-5 0.0 1.1223834036052236E-4 1.2827238898345414E-4 22-23 3.2068097245863535E-5 9.62042917375906E-5 0.0 3.367150210815671E-4 1.2827238898345414E-4 24-25 3.2068097245863535E-5 9.62042917375906E-5 0.0 5.451576531796802E-4 1.2827238898345414E-4 26-27 3.2068097245863535E-5 9.62042917375906E-5 0.0 7.696343339007249E-4 1.2827238898345414E-4 28-29 3.2068097245863535E-5 9.62042917375906E-5 0.0 0.002132528466849925 1.2827238898345414E-4 30-31 3.2068097245863535E-5 9.62042917375906E-5 0.0 0.007151185685827569 1.2827238898345414E-4 32-33 3.2068097245863535E-5 1.2827238898345414E-4 0.0 0.02065185462633612 1.2827238898345414E-4 34-35 3.2068097245863535E-5 1.2827238898345414E-4 0.0 0.04396536132407891 1.2827238898345414E-4 36-37 3.2068097245863535E-5 1.2827238898345414E-4 0.0 0.08071540076783852 1.2827238898345414E-4 38-39 3.2068097245863535E-5 1.2827238898345414E-4 0.0 0.13311467166757954 1.2827238898345414E-4 40-41 3.2068097245863535E-5 1.2827238898345414E-4 0.0 0.18694097289476147 1.2827238898345414E-4 42-43 3.2068097245863535E-5 1.2827238898345414E-4 0.0 0.24198586181728624 1.2827238898345414E-4 44-45 3.2068097245863535E-5 1.443064376063859E-4 0.0 0.31203862025087514 1.2827238898345414E-4 46-47 3.2068097245863535E-5 1.6034048622931769E-4 0.0 0.3935076213039914 1.2827238898345414E-4 48-49 3.2068097245863535E-5 1.6034048622931769E-4 0.0 0.4802518243540523 1.2827238898345414E-4 50-51 3.2068097245863535E-5 1.924085834751812E-4 0.0 0.5701387009342078 1.2827238898345414E-4 52-53 3.2068097245863535E-5 1.924085834751812E-4 0.0 0.657043244470498 1.443064376063859E-4 54-55 3.2068097245863535E-5 2.2447668072104476E-4 0.0 0.7548669751190047 1.6034048622931769E-4 56-57 3.2068097245863535E-5 2.2447668072104476E-4 0.0 0.8731180837131265 1.6034048622931769E-4 58-59 3.2068097245863535E-5 2.2447668072104476E-4 0.0 1.0066336065962793 1.6034048622931769E-4 60-61 3.2068097245863535E-5 2.2447668072104476E-4 0.0 1.1438048925654605 1.6034048622931769E-4 62-63 3.2068097245863535E-5 2.2447668072104476E-4 0.0 1.2788757181650379 1.6034048622931769E-4 64-65 3.2068097245863535E-5 2.2447668072104476E-4 0.0 1.420312061067919 1.6034048622931769E-4 66-67 3.2068097245863535E-5 2.565447779669083E-4 0.0 1.58411590179979 1.6034048622931769E-4 68-69 3.2068097245863535E-5 2.725788265898401E-4 0.0 1.7691969250542914 1.7637453485224943E-4 70-71 3.2068097245863535E-5 3.0464692383570357E-4 0.0 1.965501782344845 1.924085834751812E-4 72-73 3.2068097245863535E-5 3.2068097245863537E-4 0.0 2.167081841632343 1.924085834751812E-4 74-75 3.2068097245863535E-5 3.2068097245863537E-4 0.0 2.370826497483937 1.924085834751812E-4 76-77 3.2068097245863535E-5 3.367150210815672E-4 0.0 2.59868636246442 1.924085834751812E-4 78-79 3.2068097245863535E-5 3.527490697044989E-4 0.0 2.850549198233433 1.924085834751812E-4 80-81 3.2068097245863535E-5 3.527490697044989E-4 0.0 3.121171870891275 1.924085834751812E-4 82-83 3.2068097245863535E-5 3.527490697044989E-4 0.0 3.3974385286643893 1.924085834751812E-4 84-85 3.2068097245863535E-5 3.848171669503624E-4 0.0 3.680198976129791 1.924085834751812E-4 86-87 3.2068097245863535E-5 3.848171669503624E-4 0.0 3.972499682525837 2.0844263209811298E-4 88-89 3.2068097245863535E-5 3.848171669503624E-4 0.0 4.2909038200800165 2.2447668072104476E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3775 0.0 54.87119 1 GTATCAA 7405 0.0 39.979713 1 ATCAACG 8480 0.0 33.440197 3 CAACGCA 8670 0.0 32.982033 5 TCAACGC 8780 0.0 32.904728 4 AACGCAG 9070 0.0 31.82277 6 TATCAAC 9230 0.0 31.398342 2 ACGCAGA 10210 0.0 27.442759 7 CGCAGAG 10245 0.0 26.977106 8 GTACATG 7665 0.0 23.95164 1 GCAGAGT 11250 0.0 23.087202 9 TACATGG 8000 0.0 21.56729 2 GAGTACT 7155 0.0 21.370356 12-13 ACATGGG 7735 0.0 21.046131 3 CAGAGTA 11270 0.0 20.217402 10-11 AGAGTAC 10390 0.0 20.052673 10-11 GTGGTAT 1940 0.0 19.570837 1 GTACTTT 8590 0.0 18.313354 14-15 GGCGTCG 455 0.0 17.773327 8 AGTACTT 8150 0.0 17.769289 12-13 >>END_MODULE