FastQCFastQC Report
Sat 18 Jun 2016
SRR1931144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118364
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91890.29467374559224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73790.23663048957722702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.1611101205632184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45900.053.793521
GTATCAA84750.040.978731
ATCAACG102400.033.021413
CAACGCA103500.032.57665
TCAACGC105550.031.9888844
AACGCAG109600.031.0234586
TATCAAC110100.031.0139562
ACGCAGA126800.026.2534587
CGCAGAG128400.025.667428
GCAGAGT140250.022.7199529
GTACATG82150.021.8906961
GAGTACT93850.021.02444312-13
GTGGTAT22650.020.7942071
TGGTATC22450.020.51972
AGAGTAC128600.020.30997810-11
CAGAGTA139550.020.14557310-11
TACATGG85900.019.958582
GTACTAG10750.019.029851
GTACTTT104400.018.9225914-15
ACATGGG82050.018.6956063
Produced by FastQC (version 0.11.5)