##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931144_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3118364 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25458605858713 31.0 31.0 34.0 30.0 34.0 2 31.276416736468224 31.0 31.0 34.0 28.0 34.0 3 31.432399809643776 31.0 31.0 34.0 28.0 34.0 4 35.031849072141675 35.0 35.0 37.0 32.0 37.0 5 34.927480884207235 35.0 35.0 37.0 32.0 37.0 6 35.0533382247871 36.0 35.0 37.0 32.0 37.0 7 34.90391820839389 36.0 35.0 37.0 32.0 37.0 8 34.96735339427982 36.0 35.0 37.0 32.0 37.0 9 36.33999879423954 38.0 35.0 39.0 32.0 39.0 10-11 36.21426860366525 38.0 35.0 39.0 31.5 39.0 12-13 36.14810811053488 38.0 35.0 39.0 31.5 39.0 14-15 37.08755696897475 39.0 36.0 40.0 31.0 41.0 16-17 37.13970129850139 39.0 36.0 40.0 31.0 41.0 18-19 37.23051478275147 39.0 36.0 40.0 31.5 41.0 20-21 37.219488327853966 39.0 36.0 40.0 31.0 41.0 22-23 37.114644089015904 39.0 36.0 40.0 31.0 41.0 24-25 36.97655373137966 39.0 36.0 40.0 31.0 41.0 26-27 36.73198141717901 39.0 35.5 40.0 30.5 41.0 28-29 36.44876031149667 38.0 35.0 40.0 30.0 41.0 30-31 36.246437234395984 38.0 35.0 40.0 30.0 41.0 32-33 36.1067824346356 38.0 35.0 40.0 30.0 41.0 34-35 35.87483100112752 38.0 34.0 40.0 29.0 41.0 36-37 36.08792847788135 38.0 35.0 40.0 30.0 41.0 38-39 36.07208555511801 38.0 35.0 40.0 30.0 41.0 40-41 35.914716819460466 38.0 35.0 40.0 29.0 41.0 42-43 35.76261093958242 38.0 34.5 40.0 28.5 41.0 44-45 35.520096595522524 38.0 34.0 40.0 27.5 41.0 46-47 35.33580797495097 38.0 34.0 40.0 27.0 41.0 48-49 35.08441028693251 38.0 34.0 40.0 26.0 41.0 50-51 34.746858609193794 38.0 33.0 40.0 25.0 41.0 52-53 34.63180549159752 38.0 33.0 40.0 25.0 41.0 54-55 34.415897406460566 38.0 33.0 40.0 24.0 41.0 56-57 34.22030542297179 38.0 33.0 40.0 24.0 41.0 58-59 33.982063190826985 37.0 33.0 40.0 23.5 41.0 60-61 33.705897707900675 37.0 32.0 40.0 23.0 41.0 62-63 33.458728358844574 36.5 32.0 40.0 22.5 41.0 64-65 33.222759915134986 36.0 32.0 39.0 22.0 41.0 66-67 32.991541237648974 36.0 31.0 39.0 22.0 41.0 68-69 32.69545858020423 35.5 31.0 39.0 21.5 40.0 70-71 31.896664885818332 35.0 30.0 38.5 18.0 40.0 72-73 31.865096088846585 35.0 31.0 38.0 20.0 40.0 74-75 31.553127216707225 35.0 30.5 37.0 18.5 39.5 76-77 30.8308893701954 34.0 29.5 36.0 18.0 39.0 78-79 30.855580201669852 34.0 30.0 36.0 18.0 39.0 80-81 30.579973826019028 34.0 30.0 36.0 16.0 38.5 82-83 30.215808834375974 34.0 30.0 36.0 12.5 37.0 84-85 29.853204917706847 34.0 29.0 35.0 9.0 37.0 86-87 29.516606143477798 34.0 29.0 35.0 7.0 36.5 88-89 29.193751435047353 34.0 29.0 35.0 3.5 36.0 90-91 28.87779200888671 34.0 29.0 35.0 2.0 36.0 92-93 28.547346300816713 34.0 29.0 35.0 2.0 35.5 94-95 28.17419133879175 33.5 29.0 35.0 2.0 35.0 96-97 27.81015542124011 33.0 27.5 35.0 2.0 35.0 98-99 27.341358000541312 33.0 27.0 35.0 2.0 35.0 100-101 26.67439721597607 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8123079778799855 1101 2 0.7218708034362393 1101 3 0.5533513847517888 1101 4 0.8038336505402768 1101 5 0.8241346509388592 1101 6 0.8333575557398163 1101 7 0.8363528234512003 1101 8 0.7216624488882957 1101 9 1.1293528835479663 1101 10-11 0.8846406384075038 1101 12-13 0.9994329180964385 1101 14-15 1.3096582100992649 1101 16-17 1.0566542869927105 1101 18-19 0.8890408177369054 1101 20-21 0.8861810566413482 1101 22-23 0.8286251214959179 1101 24-25 0.7638989798154086 1101 26-27 0.8833353478700801 1101 28-29 0.8724945503663122 1101 30-31 1.0348304302610885 1101 32-33 1.1455942385591342 1101 34-35 0.9072644636692644 1101 36-37 1.1706598315615935 1101 38-39 1.022879527691046 1101 40-41 1.0893538926410855 1101 42-43 1.1574379626654974 1101 44-45 1.001472484356178 1101 46-47 1.19216569704232 1101 48-49 1.0364885918802855 1101 50-51 0.8808605778478054 1101 52-53 0.78496835592032 1101 54-55 0.8946283648351852 1101 56-57 0.727770436041169 1101 58-59 0.9204011412985054 1101 60-61 1.1174098773135555 1101 62-63 0.9393163272018867 1101 64-65 0.9996730703887451 1101 66-67 0.6970026756811407 1101 68-69 0.633207500416276 1101 70-71 0.9075681336000514 1101 72-73 0.4346774927921082 1101 74-75 0.25326581313527186 1101 76-77 0.2129855215968881 1101 78-79 0.28786513653735923 1101 80-81 0.21135607121672706 1101 82-83 0.1581091045436409 1101 84-85 0.3302588767726107 1101 86-87 0.27761019102772266 1101 88-89 0.15066307605275142 1101 90-91 0.12146832550980236 1101 92-93 -0.03144964702946851 1101 94-95 -9.551072261828608E-4 1101 96-97 0.02662755199555633 1101 98-99 -0.09120623364480096 1101 100-101 -0.25815911333900843 1102 1 0.9206654825142842 1102 2 0.9416999005273965 1102 3 0.7207043828188056 1102 4 0.9945870383636901 1102 5 0.993765416276986 1102 6 0.9451320702782837 1102 7 0.9843898179988884 1102 8 0.9553596907967474 1102 9 1.3613308864794007 1102 10-11 1.3025593447184036 1102 12-13 1.3746304564650131 1102 14-15 1.8459048583192086 1102 16-17 1.6490534916265958 1102 18-19 1.4046827332498637 1102 20-21 1.4162595535964044 1102 22-23 1.0898805184846694 1102 24-25 0.8124007971325042 1102 26-27 1.1124569513768847 1102 28-29 1.2688393384479468 1102 30-31 1.5490383303024018 1102 32-33 1.6137921228576957 1102 34-35 1.554790138092713 1102 36-37 1.7886064104375805 1102 38-39 1.5943964227590968 1102 40-41 1.6227797898871046 1102 42-43 1.7201436951517692 1102 44-45 1.6706178850885536 1102 46-47 1.5423861645805488 1102 48-49 1.454858737534181 1102 50-51 1.1664721954086374 1102 52-53 1.289221650476179 1102 54-55 1.11188510131538 1102 56-57 1.1854039576184263 1102 58-59 1.3787367183661416 1102 60-61 1.5007268619012208 1102 62-63 1.4634367582228904 1102 64-65 1.4364448888560588 1102 66-67 1.1872381952661044 1102 68-69 1.2338784380042753 1102 70-71 1.516260475192766 1102 72-73 1.059266386817061 1102 74-75 1.0619585639851223 1102 76-77 1.0336212573270593 1102 78-79 1.062155624669117 1102 80-81 0.9821114751700364 1102 82-83 0.9981986270295948 1102 84-85 1.033671018670816 1102 86-87 1.0124555428072064 1102 88-89 0.9302334479612284 1102 90-91 0.964339432343575 1102 92-93 0.9444254344809124 1102 94-95 0.9835890097598963 1102 96-97 0.9868104224447087 1102 98-99 1.0555953428424125 1102 100-101 1.0385096268971559 1103 1 0.6611016898653084 1103 2 0.5277579992323744 1103 3 0.3036336570362721 1103 4 0.7186306734479047 1103 5 0.7620733442633707 1103 6 0.7180055278739914 1103 7 0.7302880159079095 1103 8 0.6520539130252203 1103 9 0.8574946506310326 1103 10-11 0.8110830776521141 1103 12-13 0.965570016414965 1103 14-15 1.3194198002677169 1103 16-17 1.0750151800639784 1103 18-19 0.963628861076657 1103 20-21 0.9473652059853634 1103 22-23 0.7417493256757623 1103 24-25 0.5715632809126276 1103 26-27 0.9069141681278623 1103 28-29 0.7884346331436092 1103 30-31 1.1111424830653718 1103 32-33 1.087900867050429 1103 34-35 0.8809642436514054 1103 36-37 1.1811330470581538 1103 38-39 0.981732159473502 1103 40-41 1.094406711179765 1103 42-43 1.258091687072067 1103 44-45 1.124801376690236 1103 46-47 1.0660813601094787 1103 48-49 0.9430469049384129 1103 50-51 0.7287133822181886 1103 52-53 0.2779891992642476 1103 54-55 0.5904164220609971 1103 56-57 0.8235804089704075 1103 58-59 0.925970488835695 1103 60-61 0.8993263667706657 1103 62-63 0.6390334410519216 1103 64-65 0.6934944296054653 1103 66-67 0.5486703718072832 1103 68-69 0.4737418286915016 1103 70-71 0.8104255080931324 1103 72-73 0.39292179799022975 1103 74-75 0.25092994912383304 1103 76-77 0.37838162782694695 1103 78-79 0.38427779131083994 1103 80-81 0.2799464018051623 1103 82-83 0.21510199908592398 1103 84-85 0.3679499706286933 1103 86-87 0.3652277056966362 1103 88-89 0.151915326363671 1103 90-91 0.22885244993229037 1103 92-93 -0.19251824854369914 1103 94-95 -0.19539237814675658 1103 96-97 -0.10466317568623396 1103 98-99 -0.1949414216703893 1103 100-101 -0.23045850386230882 1104 1 0.5556412677602935 1104 2 0.46833705634601586 1104 3 0.27790077338876884 1104 4 0.754055478227734 1104 5 0.42756896769822816 1104 6 0.6694916467256817 1104 7 0.5422439741867962 1104 8 0.4961214902781421 1104 9 0.7779598426759122 1104 10-11 0.6326496488826265 1104 12-13 0.6859233799474183 1104 14-15 1.1672490889450913 1104 16-17 0.9825030682264995 1104 18-19 0.8859746462104354 1104 20-21 0.7402779969737594 1104 22-23 0.4950732965460034 1104 24-25 0.4986436042908764 1104 26-27 0.23214324919975837 1104 28-29 0.6501836232343692 1104 30-31 0.9169415702007129 1104 32-33 1.000825688287982 1104 34-35 0.7800265453270256 1104 36-37 0.9790901667624183 1104 38-39 0.637014396906558 1104 40-41 0.8840411577231748 1104 42-43 1.0124020361257777 1104 44-45 0.9602559244367654 1104 46-47 0.9184335476497054 1104 48-49 0.8077112240093669 1104 50-51 0.651867732037779 1104 52-53 0.5195431631746175 1104 54-55 0.7929260015093149 1104 56-57 0.7198142924774231 1104 58-59 0.6901929073990019 1104 60-61 0.9141969396942997 1104 62-63 0.07843111341090747 1104 64-65 -0.5808145103865741 1104 66-67 -0.11187060002226445 1104 68-69 0.4777353489687499 1104 70-71 0.9017277317913397 1104 72-73 0.45744729787446303 1104 74-75 0.2864795174901502 1104 76-77 0.14034408515766117 1104 78-79 0.11674204687596657 1104 80-81 0.12848046643122757 1104 82-83 0.0335178257898292 1104 84-85 -0.06820668481357473 1104 86-87 0.09754842886182047 1104 88-89 0.20450396593704312 1104 90-91 0.4041573346000362 1104 92-93 0.4630461163707764 1104 94-95 0.5422696131711291 1104 96-97 0.55841715357478 1104 98-99 0.48471019413536354 1104 100-101 0.15145193864328377 1105 1 -0.18610666113614371 1105 2 -1.687924563400177 1105 3 -0.2860236075568565 1105 4 0.4912890773052254 1105 5 0.5468783923941274 1105 6 0.05974216502608698 1105 7 -0.06704044761961825 1105 8 -0.20146593986005001 1105 9 0.5404588154643832 1105 10-11 0.8538367082358889 1105 12-13 1.003343998411431 1105 14-15 1.2885031112727816 1105 16-17 0.9441756145453226 1105 18-19 0.699400564712036 1105 20-21 1.0137413570759435 1105 22-23 0.3618456022418286 1105 24-25 -0.06047818365294688 1105 26-27 0.8713447238309797 1105 28-29 0.5775345500180578 1105 30-31 0.8768693941017958 1105 32-33 1.1935163210910034 1105 34-35 0.6431160743834567 1105 36-37 0.8893316962868312 1105 38-39 0.686122212280857 1105 40-41 0.9409287138521307 1105 42-43 1.2864429612301933 1105 44-45 1.2195731796212002 1105 46-47 1.1306806053793679 1105 48-49 1.171678151661851 1105 50-51 0.816473072175036 1105 52-53 1.0260818163654903 1105 54-55 0.29986496599201473 1105 56-57 0.5460483443173558 1105 58-59 -0.17283974592098872 1105 60-61 0.3801991047113731 1105 62-63 0.9812210282335698 1105 64-65 1.312704825577363 1105 66-67 0.9815520970442648 1105 68-69 0.9858863607378368 1105 70-71 0.34070426267719256 1105 72-73 -0.17494815775004113 1105 74-75 -0.05475400381423867 1105 76-77 0.11143627545016344 1105 78-79 -0.20062533107946123 1105 80-81 0.048542532857602794 1105 82-83 -0.027359943391729757 1105 84-85 0.039758543961909254 1105 86-87 -0.15317537616400045 1105 88-89 -0.541371961534221 1105 90-91 -0.5635893866529393 1105 92-93 -0.23618942221502692 1105 94-95 -0.6002684397709181 1105 96-97 -0.49052948618261283 1105 98-99 -1.0870472719371627 1105 100-101 -0.649051326456064 1106 1 0.2637936095268536 1106 2 0.37280511884821976 1106 3 -0.1447489138519451 1106 4 0.4220059893729484 1106 5 0.09576773109363046 1106 6 0.526857545408852 1106 7 0.49097063288574816 1106 8 0.41594572768500626 1106 9 0.8552625095064386 1106 10-11 0.16272575592961402 1106 12-13 0.29104340528456873 1106 14-15 0.21482196832733536 1106 16-17 -0.06449166096190595 1106 18-19 0.4991139516499814 1106 20-21 0.47229508968086975 1106 22-23 0.38272994024651297 1106 24-25 0.29810150464118834 1106 26-27 0.732492768322814 1106 28-29 0.6785254338937676 1106 30-31 0.21403729642257474 1106 32-33 0.23287462204456943 1106 34-35 -1.1064660882885846 1106 36-37 -0.824569981149665 1106 38-39 -0.7001967727794067 1106 40-41 0.5185046375813585 1106 42-43 0.5868736635919731 1106 44-45 0.4969641443154771 1106 46-47 0.024130682371570344 1106 48-49 0.06948185003215457 1106 50-51 0.6864934085058891 1106 52-53 0.10807492689583142 1106 54-55 0.6729809223083691 1106 56-57 0.46436534832816534 1106 58-59 0.8784119426722512 1106 60-61 0.879821211609773 1106 62-63 0.7273702436552938 1106 64-65 0.7917566458783512 1106 66-67 0.5908968266303631 1106 68-69 0.7055543958990711 1106 70-71 0.9069845117376119 1106 72-73 0.22886734237791728 1106 74-75 0.45341840071583306 1106 76-77 0.43604069407818 1106 78-79 0.5016415486599151 1106 80-81 0.5614461525734633 1106 82-83 0.44038630251864674 1106 84-85 0.5639557202665628 1106 86-87 0.4675116258512162 1106 88-89 0.39776772593478427 1106 90-91 0.5946453723109357 1106 92-93 0.03573017344165663 1106 94-95 0.23309232883795516 1106 96-97 0.6309649417474574 1106 98-99 0.5270713567515308 1106 100-101 0.4295120369665959 1107 1 0.26414282129877975 1107 2 0.30865489107724287 1107 3 0.07008935207661082 1107 4 -1.2976589543134978 1107 5 0.04647302769116379 1107 6 0.3435994450602493 1107 7 0.4726141467950882 1107 8 0.39517078582801446 1107 9 0.7112404490255884 1107 10-11 0.7531091282944828 1107 12-13 0.8810282289755733 1107 14-15 1.1640309228044785 1107 16-17 1.0118239981791035 1107 18-19 0.780648886472207 1107 20-21 0.08881722849431384 1107 22-23 0.4294809820700536 1107 24-25 0.4077606340274329 1107 26-27 0.7733389220912201 1107 28-29 0.8648076778649383 1107 30-31 1.0061869298765558 1107 32-33 1.1057514928038685 1107 34-35 1.0063393583032436 1107 36-37 1.0205445537417006 1107 38-39 0.7950609465190084 1107 40-41 0.40107541992691154 1107 42-43 0.25336100757112945 1107 44-45 -0.09891299631225081 1107 46-47 0.40072437169539654 1107 48-49 0.6774093236492291 1107 50-51 -0.812390794184509 1107 52-53 0.47541197740570595 1107 54-55 0.9064143360631647 1107 56-57 0.864611249511853 1107 58-59 0.9227844530854341 1107 60-61 1.1140627777159082 1107 62-63 1.1066937608192333 1107 64-65 1.232042696308369 1107 66-67 1.0472161067588601 1107 68-69 0.9591582639121725 1107 70-71 1.3995705841007577 1107 72-73 0.8527094728830598 1107 74-75 0.6860029797059255 1107 76-77 0.7736283507812765 1107 78-79 0.8206976080148003 1107 80-81 0.861355577349407 1107 82-83 0.9960124907736088 1107 84-85 0.8976740625264412 1107 86-87 0.8150253492168709 1107 88-89 0.9661089371689933 1107 90-91 1.0288578669160948 1107 92-93 1.114967827971654 1107 94-95 0.9572856546985307 1107 96-97 1.0053524945461554 1107 98-99 0.9594434775232159 1107 100-101 0.9600129183346553 1108 1 0.43907274869286894 1108 2 0.3880484318471815 1108 3 0.1891779201318684 1108 4 0.5426190817012682 1108 5 0.40718542089726384 1108 6 0.44151458167277724 1108 7 0.506498841915338 1108 8 0.3884197608854194 1108 9 0.6220739295539985 1108 10-11 0.4875441199740891 1108 12-13 0.6739308501326278 1108 14-15 0.9002525916248416 1108 16-17 0.6800389246900238 1108 18-19 0.6036859238535612 1108 20-21 0.4236933986952067 1108 22-23 0.36286610483075066 1108 24-25 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1.4008215025960666 2311 76-77 1.4338779784124505 2311 78-79 1.3843034731751267 2311 80-81 1.3454157324796654 2311 82-83 1.358228647365241 2311 84-85 1.3946859783289334 2311 86-87 1.2569976393554185 2311 88-89 1.3743562041223143 2311 90-91 1.6413610285382205 2311 92-93 1.7335809999920002 2311 94-95 1.8480600413438353 2311 96-97 1.799805252885811 2311 98-99 2.0132644735044423 2311 100-101 2.271754129025151 2312 1 -0.21906639791757598 2312 2 -0.17236971675395552 2312 3 -0.34990804827286226 2312 4 0.10573317828842477 2312 5 -1.3054480811420532 2312 6 -0.08794830130599962 2312 7 0.1696080129361235 2312 8 -2.5347813838929696 2312 9 -4.971351270922948 2312 10-11 -0.6001519111708475 2312 12-13 -5.653791868626492 2312 14-15 -2.2368625425296713 2312 16-17 -3.216711608806385 2312 18-19 -2.5806268508906953 2312 20-21 -3.4740083448273538 2312 22-23 -2.0544377936590834 2312 24-25 -3.377853961200401 2312 26-27 -3.4524547117266167 2312 28-29 -6.4506607363811455 2312 30-31 -6.90260934966156 2312 32-33 -1.8284125632432833 2312 34-35 0.4314219136609907 2312 36-37 1.0149723755965283 2312 38-39 0.9476419223422639 2312 40-41 -2.781741507088725 2312 42-43 -5.028891112460329 2312 44-45 -3.7056901453841107 2312 46-47 0.15996259182328032 2312 48-49 -1.4161108417411938 2312 50-51 -6.906360551034922 2312 52-53 -1.3607397006851656 2312 54-55 0.7565140441783527 2312 56-57 1.238015878535137 2312 58-59 1.2890492982417001 2312 60-61 1.4382398155622198 2312 62-63 1.2771890847389713 2312 64-65 1.444784603662093 2312 66-67 1.059128223762876 2312 68-69 1.219874212603628 2312 70-71 1.7588311809673627 2312 72-73 1.1932955706759891 2312 74-75 1.0054451160921403 2312 76-77 1.1347381136207524 2312 78-79 1.1825128139959666 2312 80-81 1.086655812305164 2312 82-83 -0.1439406414762061 2312 84-85 0.34485344170357024 2312 86-87 1.0496849430790611 2312 88-89 1.2152416328749638 2312 90-91 1.4439414341631327 2312 92-93 1.3934934394654022 2312 94-95 1.4293381775112834 2312 96-97 1.7732524458438412 2312 98-99 1.776474355777303 2312 100-101 1.8094217857755126 2313 1 -0.31582583963139754 2313 2 -0.4147423586492138 2313 3 -0.681990913765155 2313 4 -1.4947590772382924 2313 5 -2.5770697731031156 2313 6 -2.021679702300922 2313 7 -2.339214663535998 2313 8 -3.6057494616425743 2313 9 -2.8264035823675187 2313 10-11 -1.516505651048746 2313 12-13 -4.715401886249538 2313 14-15 -2.8944419391820446 2313 16-17 -6.354242002982602 2313 18-19 -4.218250193618026 2313 20-21 -4.154720776881959 2313 22-23 -2.093734582100744 2313 24-25 -0.8904763744422155 2313 26-27 -3.8948080203232465 2313 28-29 -4.991130535537916 2313 30-31 -4.000008277407069 2313 32-33 -8.331279096559758 2313 34-35 -4.393937053643427 2313 36-37 -4.889087988910504 2313 38-39 -3.453377165572192 2313 40-41 -1.8955254054253174 2313 42-43 -3.082487106005985 2313 44-45 -6.255720632508876 2313 46-47 -3.9702062771043956 2313 48-49 -2.3313938143102177 2313 50-51 -0.15064421514116333 2313 52-53 -1.1348505548451655 2313 54-55 -4.758186698666464 2313 56-57 -5.786195360060077 2313 58-59 -1.9796203477358638 2313 60-61 -7.065342421597872 2313 62-63 -1.7796117748492222 2313 64-65 -3.5218003922109915 2313 66-67 -3.6230676671675788 2313 68-69 -4.720196279577536 2313 70-71 -4.314218944327287 2313 72-73 -1.3343008541509427 2313 74-75 0.2922341526246406 2313 76-77 -2.796905662916057 2313 78-79 -0.8759778502515552 2313 80-81 0.6975304209917645 2313 82-83 0.9668001212967852 2313 84-85 0.8633260367527598 2313 86-87 0.6820436798710752 2313 88-89 -3.4796175686598048 2313 90-91 0.11862336422120734 2313 92-93 0.9511494257238908 2313 94-95 0.9559567835804543 2313 96-97 1.3387142514252162 2313 98-99 1.3661920152584237 2313 100-101 1.9007548910750671 2314 1 -28.768864513664546 2314 2 -14.827502267867253 2314 3 -5.772566553938468 2314 4 -4.660527858329971 2314 5 -2.0916155194179638 2314 6 -1.5742575890985648 2314 7 -0.5673616569565993 2314 8 -4.374966123342215 2314 9 -3.9940751167202357 2314 10-11 -0.6386830426720564 2314 12-13 0.43017020058936595 2314 14-15 -1.3898799163031512 2314 16-17 -1.0596132691158928 2314 18-19 -3.256523043850713 2314 20-21 -2.007601270222324 2314 22-23 -4.3413102910482735 2314 24-25 -0.7574326569666852 2314 26-27 -3.0715692133266117 2314 28-29 -2.7846130325003884 2314 30-31 -4.661169186143688 2314 32-33 -2.6425282655380045 2314 34-35 -2.369258573095067 2314 36-37 -5.365062863397561 2314 38-39 -8.020546648160852 2314 40-41 -6.390287102772923 2314 42-43 -4.10065496093824 2314 44-45 -3.2674351736702434 2314 46-47 -5.172012746465484 2314 48-49 -5.024271098187249 2314 50-51 -2.781968577260322 2314 52-53 -3.758816076039089 2314 54-55 -5.4524738539357145 2314 56-57 -2.1945078700896516 2314 58-59 -0.7890126129292341 2314 60-61 -0.36503752258133915 2314 62-63 0.3201222981889913 2314 64-65 -1.1683697051565929 2314 66-67 -0.39511553402070376 2314 68-69 0.8835037731493571 2314 70-71 1.4039672503954392 2314 72-73 0.6232769180230129 2314 74-75 0.7862164088773653 2314 76-77 -1.1160409417954966 2314 78-79 -2.401963040870754 2314 80-81 -1.4269050573328954 2314 82-83 -1.1861422688364058 2314 84-85 -0.0696305667180468 2314 86-87 0.32933512206634674 2314 88-89 0.347598680394551 2314 90-91 0.7849652637650024 2314 92-93 0.6182593039935291 2314 94-95 0.7629763383820389 2314 96-97 0.8240194943128145 2314 98-99 0.7319676737715248 2314 100-101 0.670326886215026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 29.0 4 50.0 5 129.0 6 297.0 7 790.0 8 1986.0 9 3517.0 10 5395.0 11 7827.0 12 10511.0 13 13082.0 14 15698.0 15 19565.0 16 22303.0 17 24622.0 18 22654.0 19 18696.0 20 18137.0 21 19202.0 22 21283.0 23 24335.0 24 28924.0 25 35842.0 26 45507.0 27 57650.0 28 73069.0 29 91228.0 30 113124.0 31 137206.0 32 165955.0 33 201400.0 34 245513.0 35 300908.0 36 375617.0 37 466331.0 38 426385.0 39 103478.0 40 112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.816032339355104 21.79318846230683 15.417304484175595 22.97347471416247 2 14.52367331074884 22.106495585505733 40.81281082003256 22.55702028371287 3 17.68003586670823 27.119772485001022 31.410693051017695 23.789498597273052 4 12.243734996090886 18.265790221379614 39.68853439987891 29.80194038265059 5 11.74201670944026 38.8763377637647 36.62415817047246 12.757487356322574 6 28.881330082055847 39.91221037697972 18.262300360060596 12.944159180903833 7 25.20048974398114 33.68609309240358 23.67096977774243 17.442447385872846 8 24.24870861772391 37.04092915387684 20.87091179862261 17.83945042977664 9 24.790018099234086 16.509458164601696 21.773564599899178 36.92695913626504 10-11 23.184031755112617 27.60689579535936 29.700253081423465 19.508819368104557 12-13 24.118319734322228 25.16508656462171 29.6834975006125 21.033096200443566 14-15 21.360046485913767 26.868912673440303 27.378651113211927 24.392389727434 16-17 20.597435065309888 29.944339403610353 28.111359033134043 21.34686649794572 18-19 20.57056199981785 28.807541390293117 30.229585128612314 20.39231148127672 20-21 21.31717143989605 27.96888047707067 30.273293945158425 20.440654137874862 22-23 21.18314282745696 27.826450023153164 30.022777969473736 20.967629179916138 24-25 21.076394852813937 27.995333744854182 30.12484990955683 20.80342149277505 26-27 21.01669796773937 28.13360298437769 29.994461377811643 20.8552376700713 28-29 20.914092266464753 28.06624176776416 30.12258697334817 20.89707899242292 30-31 21.192429948741104 27.93321207021289 29.989885475619953 20.884472505426054 32-33 21.121965204474698 28.119776855062256 29.79801380994711 20.960244130515928 34-35 21.307412409532166 27.948040353339447 29.958523935042265 20.786023302086118 36-37 21.2079411286036 27.8384291554193 29.908548586619887 21.04508112935721 38-39 21.11322388085069 27.99784949650287 29.959534553566087 20.929392069080354 40-41 21.233835265217902 27.83533256261054 29.964483682537036 20.966348489634527 42-43 21.14770471723632 27.95938645799444 29.668223541558202 21.224685283211038 44-45 21.247296242077127 28.04340122725356 29.581059825003326 21.128242705665986 46-47 21.306815903400334 28.03539720806687 29.343031154611758 21.314755733921043 48-49 21.316138580135444 28.171421936573164 29.262951874662818 21.249487608628577 50-51 21.40041042982157 28.229005590292957 29.035695326787252 21.33488865309822 52-53 21.387200655738546 28.281322354758142 28.963838174194017 21.367638815309288 54-55 21.36533075839768 28.207511155737624 29.012023495218507 21.415134590646183 56-57 21.467826624590746 28.333309014772805 28.753982698760538 21.44488166187591 58-59 21.35897578150279 28.34422969555071 28.947711892208588 21.349082630737907 60-61 21.406432193421185 28.412584999544617 28.82525209636681 21.355730710667387 62-63 21.37550715488654 28.309597022820626 28.823795114797434 21.4911007074954 64-65 21.25201347313191 28.224623824288038 28.85097532678599 21.672387375794063 66-67 21.28151575477061 28.266241632746 28.75728088397648 21.69496172850691 68-69 21.308086630674776 28.320508483001127 28.725313031640688 21.64609185468341 70-71 21.397392726573024 28.100490667692902 28.628567763453272 21.8735488422808 72-73 21.377031084691506 28.151049491970266 28.693201223902204 21.778718199436025 74-75 21.298604926802188 28.35821469882447 28.548733280719716 21.794447093653623 76-77 21.251415806493405 28.325445650347426 28.451745851350257 21.971392691808912 78-79 21.20320142228425 28.388475495484172 28.373563830264843 22.034759251966737 80-81 21.225232730916257 28.443077911764043 28.304174525378688 22.027514831941012 82-83 21.328394824185867 28.297256561963856 28.462680584283355 21.91166802956692 84-85 21.347780438717226 28.376626333551826 28.252426592918596 22.02316663481236 86-87 21.145408425049386 28.423478026629724 28.345872136278512 22.085241412042382 88-89 21.269716199609523 28.32428658657041 28.220382086351055 22.185615127469013 90-91 21.253015842321734 28.2375519289285 28.314099446674007 22.19533278207576 92-93 21.304219898100303 28.273116839906837 28.235243184305364 22.187420077687495 94-95 21.08107787424822 28.19460280408672 28.28663890725246 22.437680414412597 96-97 21.067150897109048 28.222713935254223 28.23560536822358 22.47452979941315 98-99 20.99980774839701 28.230985410524518 28.19314439442268 22.576062446655794 100-101 21.02354239219618 28.227604518340865 28.0763186364484 22.67253445301455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 477.0 1 339.5 2 237.0 3 270.0 4 235.0 5 256.5 6 454.0 7 895.5 8 1531.5 9 1966.0 10 1964.0 11 1902.5 12 2032.5 13 2287.0 14 2626.5 15 2906.5 16 3178.0 17 3531.5 18 3919.0 19 4345.5 20 5069.5 21 6088.0 22 7460.0 23 9627.0 24 12643.5 25 16684.5 26 21886.5 27 28380.5 28 35935.5 29 44529.5 30 53513.0 31 61647.5 32 70607.5 33 80031.0 34 88415.5 35 95180.5 36 103358.0 37 111359.0 38 114336.5 39 115314.0 40 115600.5 41 115312.5 42 114433.0 43 113593.5 44 113316.5 45 113834.5 46 112211.5 47 108935.0 48 107223.0 49 104163.5 50 99764.0 51 95009.0 52 89548.5 53 83728.5 54 78045.5 55 71286.5 56 63939.5 57 56182.0 58 48872.5 59 42667.5 60 35584.0 61 28480.0 62 22091.5 63 16775.5 64 12574.5 65 9089.5 66 6690.5 67 5302.0 68 4195.5 69 3240.0 70 2451.0 71 1808.0 72 1326.0 73 1016.0 74 784.5 75 585.0 76 436.0 77 301.5 78 206.0 79 144.5 80 107.5 81 86.5 82 65.0 83 43.0 84 37.0 85 32.0 86 19.5 87 11.0 88 9.5 89 8.5 90 5.0 91 3.5 92 2.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1565564507543058 2 0.0 3 0.004874350781371257 4 3.2068097245863537E-4 5 2.2447668072104476E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.021180978230892867 26-27 0.00833770528392452 28-29 0.006750334470254275 30-31 0.0024371753906856286 32-33 0.001026179111867633 34-35 0.0019080517861288803 36-37 0.0013308260357033368 38-39 7.856683825236566E-4 40-41 0.002533379682423219 42-43 0.0014270303274409274 44-45 6.092938476714071E-4 46-47 0.037487605680414475 48-49 0.020202901264894026 50-51 0.08356946142272037 52-53 0.0015392686678014497 54-55 0.03636522227680925 56-57 0.001042213160490565 58-59 0.001443064376063859 60-61 0.003591626891536716 62-63 0.003543524745667921 64-65 0.009828871805857174 66-67 0.005820359650124232 68-69 0.002212698709964584 70-71 0.0052591679483216195 72-73 0.004521601711666759 74-75 0.0010582472091134967 76-77 0.0 78-79 0.0 80-81 3.0464692383570357E-4 82-83 4.489533614420895E-4 84-85 0.0 86-87 0.0014109962788179957 88-89 0.00371989928052017 90-91 0.0012666898412116096 92-93 0.0032549118704551486 94-95 7.215321880319295E-4 96-97 4.489533614420895E-4 98-99 0.0017477112998995627 100-101 0.0030625032869799674 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3118364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.31589515360143 #Duplication Level Percentage of deduplicated Percentage of total 1 79.73458884832777 50.48466867636239 2 12.383256377066774 15.681139248610524 3 3.658591401900572 6.949409688378127 4 1.4884013148988182 3.7695784640246437 5 0.7586076731361768 2.4015961947503857 6 0.4382159307273837 1.6647620354743733 7 0.3016319968842715 1.336866992278718 8 0.2002395567680153 1.0142677425541824 9 0.14992346016924135 0.8543284276626743 >10 0.7974630485583892 9.175375655176566 >50 0.06230928973594212 2.735449967165443 >100 0.02574971492190844 2.8069751032824812 >500 7.149708337926355E-4 0.29812882071415936 >1k 1.5320803546984714E-4 0.12927538211390457 >5k 1.5320803546984714E-4 0.6981776014515826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9189 0.29467374559224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7379 0.23663048957722702 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5024 0.1611101205632184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.2068097245863535E-5 0.0 0.0 0.0 6 0.0 3.2068097245863535E-5 0.0 0.0 0.0 7 0.0 3.2068097245863535E-5 0.0 0.0 0.0 8 0.0 3.2068097245863535E-5 0.0 0.0 0.0 9 0.0 3.2068097245863535E-5 0.0 6.413619449172707E-5 0.0 10-11 0.0 3.2068097245863535E-5 0.0 1.2827238898345414E-4 0.0 12-13 0.0 3.2068097245863535E-5 0.0 1.2827238898345414E-4 0.0 14-15 0.0 3.2068097245863535E-5 0.0 1.2827238898345414E-4 8.017024311465884E-5 16-17 0.0 3.2068097245863535E-5 0.0 1.6034048622931769E-4 1.2827238898345414E-4 18-19 0.0 3.2068097245863535E-5 0.0 1.6034048622931769E-4 1.2827238898345414E-4 20-21 0.0 3.2068097245863535E-5 0.0 2.0844263209811298E-4 1.924085834751812E-4 22-23 0.0 3.2068097245863535E-5 0.0 4.3291931281915776E-4 1.924085834751812E-4 24-25 0.0 3.2068097245863535E-5 0.0 6.413619449172707E-4 1.924085834751812E-4 26-27 0.0 3.2068097245863535E-5 0.0 8.818726742612472E-4 1.924085834751812E-4 28-29 0.0 3.2068097245863535E-5 0.0 0.002324937050325106 1.924085834751812E-4 30-31 0.0 3.2068097245863535E-5 0.0 0.008241500992186929 1.924085834751812E-4 32-33 0.0 3.2068097245863535E-5 0.0 0.022816451190431908 1.924085834751812E-4 34-35 0.0 8.017024311465884E-5 0.0 0.04805404372292651 1.924085834751812E-4 36-37 0.0 1.2827238898345414E-4 0.0 0.08749780333533866 1.924085834751812E-4 38-39 0.0 1.2827238898345414E-4 0.0 0.14390558639081263 1.924085834751812E-4 40-41 0.0 1.2827238898345414E-4 0.0 0.2015960933361211 1.924085834751812E-4 42-43 0.0 1.2827238898345414E-4 0.0 0.25939883862179014 1.924085834751812E-4 44-45 0.0 1.2827238898345414E-4 0.0 0.33501541192753637 1.924085834751812E-4 46-47 0.0 1.2827238898345414E-4 0.0 0.42265752170048143 1.924085834751812E-4 48-49 0.0 1.2827238898345414E-4 0.0 0.5159275825400755 1.924085834751812E-4 50-51 0.0 1.2827238898345414E-4 0.0 0.6118111933052075 1.924085834751812E-4 52-53 1.6034048622931767E-5 1.2827238898345414E-4 0.0 0.7034137130880167 1.924085834751812E-4 54-55 3.2068097245863535E-5 1.2827238898345414E-4 0.0 0.8067050543169432 1.924085834751812E-4 56-57 3.2068097245863535E-5 1.2827238898345414E-4 0.0 0.930584113977714 1.924085834751812E-4 58-59 6.413619449172707E-5 1.2827238898345414E-4 0.0 1.0665688803487983 1.924085834751812E-4 60-61 6.413619449172707E-5 1.2827238898345414E-4 0.0 1.2032431108106687 1.924085834751812E-4 62-63 6.413619449172707E-5 1.443064376063859E-4 0.0 1.3399013072239163 1.924085834751812E-4 64-65 6.413619449172707E-5 1.6034048622931769E-4 0.0 1.482059182314829 1.924085834751812E-4 66-67 6.413619449172707E-5 1.6034048622931769E-4 0.0 1.6471457469365347 1.924085834751812E-4 68-69 6.413619449172707E-5 1.7637453485224943E-4 0.0 1.832034361607561 2.0844263209811298E-4 70-71 6.413619449172707E-5 1.924085834751812E-4 0.0 2.0268961545220505 2.2447668072104476E-4 72-73 9.62042917375906E-5 1.924085834751812E-4 0.0 2.233783483903739 2.886128752127718E-4 74-75 9.62042917375906E-5 1.924085834751812E-4 0.0 2.443492805843064 3.2068097245863537E-4 76-77 9.62042917375906E-5 2.0844263209811298E-4 0.0 2.677317336911278 3.367150210815672E-4 78-79 9.62042917375906E-5 2.2447668072104476E-4 0.0 2.9350807025735293 3.527490697044989E-4 80-81 9.62042917375906E-5 2.565447779669083E-4 0.0 3.2117802796594623 3.527490697044989E-4 82-83 9.62042917375906E-5 2.565447779669083E-4 0.0 3.4938191949368322 3.527490697044989E-4 84-85 9.62042917375906E-5 2.565447779669083E-4 0.0 3.780315575731377 3.527490697044989E-4 86-87 9.62042917375906E-5 3.2068097245863537E-4 0.0 4.0797353997160055 3.6878311832743067E-4 88-89 9.62042917375906E-5 3.527490697044989E-4 0.0 4.404745565302832 3.848171669503624E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4590 0.0 53.79352 1 GTATCAA 8475 0.0 40.97873 1 ATCAACG 10240 0.0 33.02141 3 CAACGCA 10350 0.0 32.5766 5 TCAACGC 10555 0.0 31.988884 4 AACGCAG 10960 0.0 31.023458 6 TATCAAC 11010 0.0 31.013956 2 ACGCAGA 12680 0.0 26.253458 7 CGCAGAG 12840 0.0 25.66742 8 GCAGAGT 14025 0.0 22.719952 9 GTACATG 8215 0.0 21.890696 1 GAGTACT 9385 0.0 21.024443 12-13 GTGGTAT 2265 0.0 20.794207 1 TGGTATC 2245 0.0 20.5197 2 AGAGTAC 12860 0.0 20.309978 10-11 CAGAGTA 13955 0.0 20.145573 10-11 TACATGG 8590 0.0 19.95858 2 GTACTAG 1075 0.0 19.02985 1 GTACTTT 10440 0.0 18.92259 14-15 ACATGGG 8205 0.0 18.695606 3 >>END_MODULE