FastQCFastQC Report
Sat 18 Jun 2016
SRR1931143_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931143_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981568
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30000.041.8904461
GTATCAA51000.037.383781
CAACGCA52900.034.612115
ATCAACG52650.034.494633
TCAACGC55750.033.011854
AACGCAG57400.032.1271826
TATCAAC60450.030.9078122
ACGCAGA64250.028.0423097
CGCAGAG64700.027.2599248
GCAGAGT78450.021.8154729
GTACATG68900.020.3895951
TACATGG69150.019.6251952
ACATGGG67900.018.9547863
CAGAGTA76400.018.0748610-11
AGAGTAC67500.017.92281310-11
GAGTACT40350.017.8482212-13
GTACTAG10350.017.5438021
TAGCCCT24350.017.0352174
CTAGTAC8850.016.6971873
CATGGGG48400.016.2542154
Produced by FastQC (version 0.11.5)