##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931143_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2981568 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.877764988086806 31.0 28.0 31.0 23.0 34.0 2 28.61659234335759 31.0 28.0 33.0 18.0 34.0 3 28.681522943632345 31.0 28.0 34.0 20.0 34.0 4 31.878405590615408 35.0 32.0 37.0 19.0 37.0 5 31.9133150744843 35.0 32.0 37.0 19.0 37.0 6 32.23490358093459 35.0 32.0 37.0 23.0 37.0 7 32.01327590046579 35.0 32.0 37.0 19.0 37.0 8 32.11329474960826 35.0 32.0 37.0 22.0 37.0 9 33.18693351954408 37.0 32.0 39.0 19.0 39.0 10-11 33.163721572005066 37.0 32.0 39.0 21.0 39.0 12-13 33.21875872024384 37.0 32.0 39.0 22.0 39.0 14-15 33.958590077435765 38.0 32.0 40.0 19.0 41.0 16-17 33.90612707810119 38.0 32.0 40.0 19.0 41.0 18-19 33.91468264349497 38.0 32.0 40.0 19.0 41.0 20-21 34.085066984888485 38.0 32.0 40.0 22.5 41.0 22-23 33.975055407087815 38.0 32.0 40.0 22.0 41.0 24-25 33.87967807542877 38.0 32.0 40.0 21.0 41.0 26-27 33.554696723334835 38.0 32.0 40.0 18.0 41.0 28-29 33.498228281226524 38.0 32.0 40.0 18.0 41.0 30-31 33.25598225497457 37.5 31.5 40.0 17.5 41.0 32-33 33.09348939886664 37.0 31.0 40.0 17.5 41.0 34-35 33.10670811465645 37.0 31.0 40.0 18.0 41.0 36-37 32.78706036555262 37.0 30.5 40.0 16.0 41.0 38-39 32.59224559023977 37.0 30.0 40.0 15.5 41.0 40-41 32.524492481808224 37.0 30.0 40.0 15.0 41.0 42-43 32.786757840170004 37.0 30.5 40.0 15.0 41.0 44-45 32.84206229742203 37.0 31.0 40.0 15.0 41.0 46-47 32.621502343733226 37.0 30.5 40.0 14.0 41.0 48-49 32.4585600596733 37.0 30.0 40.0 12.5 41.0 50-51 31.95872423503338 36.5 30.0 39.5 9.0 40.5 52-53 31.91005923728723 36.0 30.0 39.5 8.5 40.5 54-55 31.874773776751027 36.0 30.0 40.0 8.0 41.0 56-57 31.630627743522872 36.0 30.0 40.0 7.0 41.0 58-59 31.46890394584326 36.0 29.5 40.0 6.5 41.0 60-61 31.296427919805954 36.0 29.0 39.5 2.0 41.0 62-63 31.155212626376457 35.0 29.0 39.0 2.0 41.0 64-65 30.83753867092751 35.0 29.0 39.0 2.0 40.5 66-67 30.588407005978063 35.0 29.0 39.0 2.0 40.0 68-69 30.39646823416404 35.0 29.0 38.5 2.0 40.0 70-71 30.05758999962436 34.0 29.0 38.0 2.0 40.0 72-73 29.710394664820658 34.0 28.5 37.5 2.0 40.0 74-75 29.293815535986433 34.0 28.0 37.0 2.0 39.0 76-77 28.948652017998583 34.0 28.0 36.0 2.0 39.0 78-79 28.503929643731084 34.0 27.5 36.0 2.0 39.0 80-81 28.095985568667224 33.0 27.0 35.5 2.0 37.5 82-83 27.73389337422457 33.0 26.0 35.0 2.0 37.0 84-85 27.38403836504819 33.0 26.0 35.0 2.0 36.5 86-87 27.046032322589994 33.0 26.0 35.0 2.0 36.0 88-89 26.69201272618971 33.0 25.5 35.0 2.0 36.0 90-91 26.325698424453172 32.0 25.0 35.0 2.0 35.5 92-93 25.967903633256057 32.0 24.0 35.0 2.0 35.0 94-95 25.652343833848498 32.0 23.5 35.0 2.0 35.0 96-97 25.25529670965076 32.0 20.0 35.0 2.0 35.0 98-99 24.8774113151201 32.0 18.5 34.5 2.0 35.0 100-101 24.266818499527766 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.177462657393409 1101 2 5.871576130361426 1101 3 5.464647486809156 1101 4 6.799960345209591 1101 5 6.290203881839876 1101 6 5.76603332153228 1101 7 6.378682568511145 1101 8 5.619393651032613 1101 9 6.419405967477903 1101 10-11 6.311068449218823 1101 12-13 6.218250166634739 1101 14-15 6.538977415626363 1101 16-17 6.611808740945367 1101 18-19 6.684857852870021 1101 20-21 5.978292032133748 1101 22-23 5.967593472921852 1101 24-25 5.746682008382852 1101 26-27 6.078301855023813 1101 28-29 5.886170885313447 1101 30-31 6.018362770858083 1101 32-33 5.863165579089539 1101 34-35 5.732296782662907 1101 36-37 5.791702624065003 1101 38-39 5.738679973179412 1101 40-41 5.899054744549815 1101 42-43 5.774632382268344 1101 44-45 5.689644569511895 1101 46-47 5.450707910398268 1101 48-49 5.430531772806226 1101 50-51 5.301638785960559 1101 52-53 5.220655590299401 1101 54-55 5.4901484969458 1101 56-57 5.358432973945117 1101 58-59 5.143364483422481 1101 60-61 5.065942235437191 1101 62-63 5.050365371200272 1101 64-65 5.071008958324786 1101 66-67 4.972610634491872 1101 68-69 5.0791467782264625 1101 70-71 4.915497775967889 1101 72-73 4.6787679937291635 1101 74-75 4.840401739119553 1101 76-77 4.65061626156351 1101 78-79 4.670332863249939 1101 80-81 4.607302805076312 1101 82-83 4.464645633535174 1101 84-85 4.341676773875662 1101 86-87 4.1705456311720965 1101 88-89 4.3856175963908335 1101 90-91 4.277303108333864 1101 92-93 4.151512334456918 1101 94-95 4.074551748688801 1101 96-97 3.964400762655483 1101 98-99 3.6245409833288775 1101 100-101 3.6025779593667977 1102 1 5.177266910303523 1102 2 5.786897848013712 1102 3 5.501135028644004 1102 4 6.885523550604738 1102 5 6.402943646642843 1102 6 5.754862741751328 1102 7 6.466259085520633 1102 8 5.940685289423573 1102 9 6.645219729148106 1102 10-11 6.590886680582948 1102 12-13 6.63431822083151 1102 14-15 6.944651989744457 1102 16-17 6.862940073840907 1102 18-19 6.91698659028911 1102 20-21 6.2035865766786 1102 22-23 6.299853141589441 1102 24-25 6.065115109784305 1102 26-27 6.255606584102939 1102 28-29 6.087937299742528 1102 30-31 6.306545129171916 1102 32-33 6.037622330150818 1102 34-35 5.975753875266314 1102 36-37 6.079058438261207 1102 38-39 6.028307083095466 1102 40-41 6.201511715725047 1102 42-43 6.254167267689283 1102 44-45 6.152307131987509 1102 46-47 6.122066009393503 1102 48-49 5.801855676977873 1102 50-51 5.909810881766173 1102 52-53 5.591832988739348 1102 54-55 5.889907102609229 1102 56-57 5.698506789279261 1102 58-59 5.7776258691413105 1102 60-61 5.741750636398706 1102 62-63 5.580197207393255 1102 64-65 5.609905874739557 1102 66-67 5.6197792462429526 1102 68-69 5.512288080415701 1102 70-71 5.407603827842493 1102 72-73 5.208603891065096 1102 74-75 5.178237052463128 1102 76-77 5.098220207532439 1102 78-79 5.073661924260897 1102 80-81 5.2874751071879835 1102 82-83 5.105550642995606 1102 84-85 4.891352471305893 1102 86-87 4.921799712113113 1102 88-89 4.897601318517687 1102 90-91 4.892130097552748 1102 92-93 4.8226878850768315 1102 94-95 4.801814221575594 1102 96-97 4.715140804428398 1102 98-99 4.437776532462415 1102 100-101 4.447066237080747 1103 1 4.761525442964992 1103 2 5.512311736071169 1103 3 5.054638399912768 1103 4 6.6132799720953415 1103 5 6.173331707410998 1103 6 5.425695942088826 1103 7 6.060275999378881 1103 8 5.366680183587992 1103 9 5.962533638866315 1103 10-11 6.040358350864089 1103 12-13 6.2288388125013725 1103 14-15 6.504862366069474 1103 16-17 6.298471471999523 1103 18-19 6.265357436477867 1103 20-21 5.503706383341797 1103 22-23 5.830293077759475 1103 24-25 5.320523172122318 1103 26-27 5.685248023316621 1103 28-29 5.653880499178669 1103 30-31 5.7618040755758955 1103 32-33 5.584467729167429 1103 34-35 5.487344487386416 1103 36-37 5.5428986272538125 1103 38-39 5.366240616896189 1103 40-41 5.482935871261716 1103 42-43 5.336767668503597 1103 44-45 5.186788334807861 1103 46-47 5.179009807260709 1103 48-49 5.026869824723498 1103 50-51 4.934875527779436 1103 52-53 4.643869240292389 1103 54-55 5.0311206248628935 1103 56-57 5.083614598212524 1103 58-59 5.205554623121898 1103 60-61 4.974287681008427 1103 62-63 4.994498254557293 1103 64-65 4.921896437957347 1103 66-67 5.0738566877604185 1103 68-69 5.0300765493857185 1103 70-71 4.783989017105103 1103 72-73 4.618566563901968 1103 74-75 4.684570163567951 1103 76-77 4.752397210233912 1103 78-79 4.7301970183988615 1103 80-81 4.7402188765087665 1103 82-83 4.62896051969793 1103 84-85 4.6227247735230215 1103 86-87 4.523401905410161 1103 88-89 4.441545200096993 1103 90-91 4.353456765636697 1103 92-93 4.2674226953966645 1103 94-95 4.252134254723174 1103 96-97 4.2043179546799685 1103 98-99 4.0817338340742815 1103 100-101 4.059873153827766 1104 1 4.515423542880846 1104 2 5.4012705568350725 1104 3 4.983663324929058 1104 4 6.546629214255219 1104 5 6.078887127962933 1104 6 5.610342498832306 1104 7 6.213418673040252 1104 8 5.480092263992692 1104 9 6.257963658335999 1104 10-11 6.20267476349175 1104 12-13 6.197255595892166 1104 14-15 6.401033506810094 1104 16-17 6.126065769057821 1104 18-19 6.470958088642551 1104 20-21 5.645176773645904 1104 22-23 5.900475416888359 1104 24-25 5.506509763994817 1104 26-27 5.811132672335827 1104 28-29 5.548814769156309 1104 30-31 5.857548205267506 1104 32-33 5.783790923620927 1104 34-35 5.575626065192083 1104 36-37 5.636761724511182 1104 38-39 5.352143096113462 1104 40-41 5.68647103284647 1104 42-43 5.743491431981148 1104 44-45 5.596857163973308 1104 46-47 5.554871127654433 1104 48-49 5.419993291149879 1104 50-51 5.289002669436353 1104 52-53 4.988416465692595 1104 54-55 5.4575986795925004 1104 56-57 5.400052725351589 1104 58-59 5.3282175565604675 1104 60-61 5.086318042566475 1104 62-63 4.984758828823811 1104 64-65 5.130749667530136 1104 66-67 5.158281897969157 1104 68-69 5.036406107693836 1104 70-71 4.986382096668354 1104 72-73 4.83416680457562 1104 74-75 4.890944402674048 1104 76-77 4.919273688726054 1104 78-79 5.018124519511023 1104 80-81 4.926008147090695 1104 82-83 4.663667115251169 1104 84-85 4.65851556694577 1104 86-87 4.6055025962938885 1104 88-89 4.60969877503064 1104 90-91 4.629169690051629 1104 92-93 4.6267676891546365 1104 94-95 4.517924947703232 1104 96-97 4.457657058608021 1104 98-99 4.230549971132547 1104 100-101 4.161247663481117 1105 1 4.503500759027492 1105 2 5.415623759430613 1105 3 4.956840153134731 1105 4 6.446719261941659 1105 5 3.7704556084107814 1105 6 5.029275561511529 1105 7 5.75705234760423 1105 8 0.8599679348147404 1105 9 1.5807233899509647 1105 10-11 2.412908539959865 1105 12-13 5.574051681833026 1105 14-15 4.35222380230654 1105 16-17 5.974030471591266 1105 18-19 6.447236144859499 1105 20-21 3.9783977262400363 1105 22-23 5.5698378670031055 1105 24-25 5.548326284423428 1105 26-27 5.737105067251658 1105 28-29 3.813546407119638 1105 30-31 5.566830143960459 1105 32-33 5.777015295165242 1105 34-35 5.67262505483864 1105 36-37 5.7755046904938965 1105 38-39 5.63468718345052 1105 40-41 5.809206336087925 1105 42-43 3.7979673181612768 1105 44-45 5.49770157823151 1105 46-47 5.6387559647381345 1105 48-49 5.408992857265169 1105 50-51 5.579647880018651 1105 52-53 5.418443715083299 1105 54-55 5.784037896637578 1105 56-57 5.675201250373551 1105 58-59 5.651699586118802 1105 60-61 5.389636749538937 1105 62-63 5.295347941026193 1105 64-65 5.503576040565715 1105 66-67 5.578457358083703 1105 68-69 5.680881564588745 1105 70-71 5.451940262005511 1105 72-73 5.393641461671269 1105 74-75 5.388903330299115 1105 76-77 5.3609886883095434 1105 78-79 5.425368298835931 1105 80-81 5.496874756417117 1105 82-83 5.299354081487376 1105 84-85 5.246242634554616 1105 86-87 5.150495163015798 1105 88-89 5.171086153329558 1105 90-91 5.144325502701335 1105 92-93 5.055645987304466 1105 94-95 5.064819784577406 1105 96-97 5.019066284458557 1105 98-99 4.972016905182585 1105 100-101 5.186340304765782 1106 1 4.181329952914659 1106 2 4.637382508234015 1106 3 4.63060150500284 1106 4 6.137292234172129 1106 5 6.1217871532014065 1106 6 5.047869594385862 1106 7 5.934794044233669 1106 8 5.2940430507706004 1106 9 6.125535807239377 1106 10-11 5.988470070568724 1106 12-13 6.2116224593950164 1106 14-15 5.831694754664383 1106 16-17 5.9294555995662925 1106 18-19 6.26171042767572 1106 20-21 5.518738673225439 1106 22-23 5.618386584641829 1106 24-25 5.558827317617617 1106 26-27 5.7505736850248645 1106 28-29 5.814764585774682 1106 30-31 5.982702992253046 1106 32-33 5.791305274643264 1106 34-35 5.274210563045024 1106 36-37 5.3920616237363035 1106 38-39 5.405235345397923 1106 40-41 5.531982613007244 1106 42-43 2.5854807244269686 1106 44-45 4.7430893829358105 1106 46-47 5.0320127727371435 1106 48-49 4.819285719072575 1106 50-51 5.015174065481613 1106 52-53 4.808995572179242 1106 54-55 5.178822798975823 1106 56-57 5.058085986430118 1106 58-59 5.133001141174674 1106 60-61 4.907750782050002 1106 62-63 4.928198975171124 1106 64-65 5.160230614276973 1106 66-67 5.249913555957487 1106 68-69 5.173855787380091 1106 70-71 5.0703142320396175 1106 72-73 4.752300554134845 1106 74-75 4.934249585867736 1106 76-77 4.938682875886297 1106 78-79 5.002629711296528 1106 80-81 4.978809169517131 1106 82-83 4.954170560033518 1106 84-85 4.869408292718187 1106 86-87 4.925198266912194 1106 88-89 5.012434553333076 1106 90-91 5.0630160862311335 1106 92-93 5.045346512827244 1106 94-95 5.016902789760547 1106 96-97 5.084969287093124 1106 98-99 4.921363258380321 1106 100-101 4.885255174162715 1107 1 2.642019730535033 1107 2 -0.5677326140420469 1107 3 0.2725527886106853 1107 4 2.047212591192711 1107 5 2.06062011110615 1107 6 3.540545463801706 1107 7 4.591141628782811 1107 8 4.003187511578069 1107 9 4.581948006635525 1107 10-11 2.3284908678839074 1107 12-13 4.544119064996259 1107 14-15 2.712182884041013 1107 16-17 1.0737984095868676 1107 18-19 1.9022403730309634 1107 20-21 1.7540726571028138 1107 22-23 3.916542918662902 1107 24-25 3.7294783545870054 1107 26-27 2.7267960335673394 1107 28-29 3.5578594862409716 1107 30-31 2.962215305572883 1107 32-33 3.554852281238027 1107 34-35 4.111422121567379 1107 36-37 4.279518360752469 1107 38-39 4.239791431508973 1107 40-41 2.7349113327525885 1107 42-43 3.3990961841413565 1107 44-45 3.949110784955547 1107 46-47 4.208401156004907 1107 48-49 1.6085574409876067 1107 50-51 3.5073304494237902 1107 52-53 3.7068580546421046 1107 54-55 4.201050636012653 1107 56-57 2.7387430233495884 1107 58-59 1.7742723205510451 1107 60-61 2.9028022360959866 1107 62-63 3.8283915481371587 1107 64-65 3.983687499172593 1107 66-67 4.0251484794345735 1107 68-69 4.080696154328372 1107 70-71 3.754725947295359 1107 72-73 3.701331731740428 1107 74-75 3.84190571525944 1107 76-77 3.699796794404712 1107 78-79 3.6401881507632012 1107 80-81 3.772115575288005 1107 82-83 3.5095929325004356 1107 84-85 3.4950178834076198 1107 86-87 3.5122833037242884 1107 88-89 3.5644432179391643 1107 90-91 3.6015116529085205 1107 92-93 3.5584089551648503 1107 94-95 3.633997113779511 1107 96-97 3.7252027200017643 1107 98-99 3.714648094471954 1107 100-101 3.7983660069925236 1108 1 -1.930935874900225 1108 2 -1.3027845800000897 1108 3 -2.089733194954565 1108 4 -3.8101204204653882 1108 5 -8.815409371782117 1108 6 -7.116360388695917 1108 7 -5.86063042222376 1108 8 -5.9085962865872546 1108 9 -7.787689195556528 1108 10-11 -3.430926869874863 1108 12-13 -1.9597554117647746 1108 14-15 -6.80926458688139 1108 16-17 -6.236583395619984 1108 18-19 -4.384138897856037 1108 20-21 -4.518765758586522 1108 22-23 -3.3017702479629882 1108 24-25 -8.208537176013664 1108 26-27 -9.27400056358967 1108 28-29 -6.196160871742983 1108 30-31 -6.006023905811709 1108 32-33 -2.388234031067295 1108 34-35 -0.8380653776293769 1108 36-37 -4.487344787102778 1108 38-39 -5.470593135745762 1108 40-41 -3.0634087784508424 1108 42-43 -4.0327969009308475 1108 44-45 -1.4556867231135833 1108 46-47 -2.2046972642164384 1108 48-49 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-6.282783497791936 2313 48-49 -3.9339140223624582 2313 50-51 -3.7906173514763033 2313 52-53 -1.9388696234426845 2313 54-55 -4.127867021672127 2313 56-57 -3.024951713656442 2313 58-59 -2.784911056298842 2313 60-61 -1.4208129772216367 2313 62-63 -4.426162117375412 2313 64-65 -5.4290181230276 2313 66-67 -7.339896135846221 2313 68-69 -8.847807793306423 2313 70-71 -8.154063185159337 2313 72-73 -5.514137494357048 2313 74-75 -5.740814233662686 2313 76-77 -4.299826869602516 2313 78-79 -5.292799386026886 2313 80-81 -6.586417129607476 2313 82-83 -6.587131906194173 2313 84-85 -5.0084690642219805 2313 86-87 -4.295515416214251 2313 88-89 -7.440227241502196 2313 90-91 -6.588903977752739 2313 92-93 -5.431062721009706 2313 94-95 -6.331674555127867 2313 96-97 -6.96544649851708 2313 98-99 -5.3669454418924865 2313 100-101 -5.271237983726255 2314 1 -10.457089118230115 2314 2 -13.29801131031035 2314 3 -12.14720834901719 2314 4 -25.015848344093072 2314 5 -17.643329437325523 2314 6 -16.159868385703717 2314 7 -17.208733581843322 2314 8 -11.048011382943184 2314 9 -13.052634160469434 2314 10-11 -11.923428561892106 2314 12-13 -13.317759543848982 2314 14-15 -14.436409886635602 2314 16-17 -15.544083484673525 2314 18-19 -13.925014472251364 2314 20-21 -14.481195851558418 2314 22-23 -14.727943083071455 2314 24-25 -14.322896168640032 2314 26-27 -13.358811591979936 2314 28-29 -16.128661851331913 2314 30-31 -15.552271189678772 2314 32-33 -12.41016791771995 2314 34-35 -13.842402284978075 2314 36-37 -13.572248431173593 2314 38-39 -13.183967196230924 2314 40-41 -14.84398798266842 2314 42-43 -16.11910402877611 2314 44-45 -12.686301548746751 2314 46-47 -11.545289648357787 2314 48-49 -11.052004886721981 2314 50-51 -10.425027717229948 2314 52-53 -10.185220287703793 2314 54-55 -11.005757787622596 2314 56-57 -9.431545120249847 2314 58-59 -8.595227605421364 2314 60-61 -8.855042926376957 2314 62-63 -11.461835399317351 2314 64-65 -8.909582334935095 2314 66-67 -10.183917785838023 2314 68-69 -13.969342974543096 2314 70-71 -13.398937713615954 2314 72-73 -11.687993489108562 2314 74-75 -9.723920679455698 2314 76-77 -9.802861148756195 2314 78-79 -9.843316033558459 2314 80-81 -11.202293790260256 2314 82-83 -11.11722867509691 2314 84-85 -10.868646200518523 2314 86-87 -11.498648104421566 2314 88-89 -9.815411758477758 2314 90-91 -9.441290397303339 2314 92-93 -10.281234936853563 2314 94-95 -9.661180869708808 2314 96-97 -9.646109119478208 2314 98-99 -10.195057628213629 2314 100-101 -9.79887452628817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93543.0 3 15750.0 4 11091.0 5 8978.0 6 8979.0 7 9908.0 8 11376.0 9 12761.0 10 14212.0 11 15917.0 12 17573.0 13 18871.0 14 20665.0 15 22272.0 16 23742.0 17 24725.0 18 24112.0 19 23608.0 20 24124.0 21 26034.0 22 30313.0 23 36896.0 24 45158.0 25 54573.0 26 64704.0 27 74535.0 28 83551.0 29 94202.0 30 106828.0 31 120033.0 32 138859.0 33 166577.0 34 201540.0 35 246932.0 36 304902.0 37 372616.0 38 336406.0 39 74647.0 40 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.022140117413684 19.76997010476918 14.15157227577151 24.056317502045633 2 14.562612668222338 20.47785622668636 41.05454260444022 23.904988500651076 3 18.1165749028699 25.205831294137848 30.517499517032647 26.1600942859596 4 11.712610593012661 17.412700364306392 39.88688480136627 30.98780424131467 5 12.780843592149168 37.545333136577405 35.81900158021003 13.854821691063393 6 30.201214295978268 37.58015736695724 18.740036984860918 13.478591352203582 7 26.492201164394206 32.197356726426456 22.918533705414777 18.391908403764553 8 24.660457844659465 35.690440437751505 21.284713081506233 18.3643886360828 9 25.171163306079276 16.353541628191998 21.25030639826738 37.224988667461346 10-11 23.731761887937626 26.519753112955723 29.626159843845084 20.122325155261564 12-13 24.654842454063136 23.973137715076113 29.032060249957286 22.339959580903464 14-15 21.935976682279094 25.687072974841396 26.622844629631526 25.75410571324798 16-17 21.729662986660067 28.638368455224985 27.28649365995973 22.345474898155217 18-19 21.587154961748727 28.04914302029711 28.747642361081954 21.616059656872213 20-21 22.573210205012582 27.361353460134037 28.54051705365746 21.524919281195924 22-23 22.21544407688767 27.249526359841624 28.26502831768857 22.270001245582126 24-25 22.05405507034035 27.316655464227512 28.27310427534767 22.356185190084467 26-27 22.05059977808765 27.478315172643846 28.26125660975306 22.209828439515448 28-29 21.968679804025896 27.50496004679578 28.329762480203048 22.196597668975283 30-31 22.24301359710021 27.286468671358943 28.298397051042734 22.172120680498118 32-33 22.084240984372734 27.420154687911296 28.18797038931179 22.30763393840418 34-35 22.408953188401902 27.503745330932738 28.149554591137505 21.937746889527855 36-37 22.281461208932132 27.27377615119679 28.080740801946526 22.36402183792455 38-39 22.19161159973352 27.421393068997503 28.202134675282124 22.184860655986853 40-41 22.365742250237485 27.283156579503633 28.14391857246616 22.207182597792723 42-43 22.296595563074035 27.308090981527748 28.121937059436032 22.27337639596218 44-45 22.297533992267027 27.446633336718364 27.978457786308425 22.277374884706187 46-47 22.281689091101985 27.32542446172626 27.89411521401358 22.49877123315817 48-49 22.25258099460643 27.35786294536311 28.083407015282592 22.306149044747873 50-51 22.116154943754765 27.518262350427992 27.94604136562316 22.41954134019409 52-53 22.2238206523363 27.54561932601607 27.84362675698151 22.386933264666116 54-55 22.195639695486605 27.38278771206486 27.887433270773453 22.534139321675077 56-57 22.161108983449786 27.514351617834038 27.71984107379515 22.60469832492103 58-59 22.1288142530157 27.533303164694683 27.93122290859895 22.406659673690665 60-61 22.32092057792964 27.45461438796931 27.77950705210764 22.44495798199341 62-63 22.17034795114517 27.41091264730504 27.78033236203233 22.63840703951746 64-65 22.12131334921759 27.42023660033915 27.784608635456244 22.673841414987013 66-67 22.14951998411574 27.415541084422696 27.72205765556915 22.712881275892414 68-69 22.153578251443538 27.373063435078453 27.751371090647602 22.721987222830403 70-71 22.108685765342262 27.1589646789877 27.747346362719217 22.985003192950824 72-73 22.16994547835233 27.25532337347329 27.7400180039496 22.834713144224782 74-75 22.08413492497907 27.342207187627448 27.703979919290788 22.86967796810269 76-77 22.063256648850537 27.30085310816322 27.675806823792044 22.960083419194195 78-79 22.14531078949063 27.268521127138474 27.64032213922339 22.945845944147507 80-81 22.107813740957777 27.3231903481658 27.588017445853996 22.98097846502243 82-83 22.203015326163953 27.18639990769957 27.77610639770752 22.83447836842896 84-85 22.314751835275935 27.15517472685513 27.568413666902785 22.96165977096615 86-87 22.048180688818768 27.160842885354285 27.734400154549554 23.056576271277397 88-89 22.214754115955095 27.006175945006117 27.690312614033957 23.08875732500483 90-91 22.185994080966793 27.04323698134673 27.666717646553764 23.10405129113272 92-93 22.23268092493614 26.920834943224502 27.732153014789535 23.114331117049822 94-95 22.048130379719662 26.982631286625026 27.74857055079743 23.22066778285788 96-97 21.986619121214073 26.92824715049263 27.77535176122094 23.30978196707236 98-99 22.02056099341018 26.82338621825831 27.654744080966793 23.501308707364714 100-101 22.02674901260008 26.756408037649987 27.601667981411122 23.615174968338806 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 131.0 1 92.0 2 68.0 3 81.0 4 70.5 5 92.0 6 140.5 7 255.0 8 450.0 9 565.0 10 543.5 11 568.0 12 650.5 13 732.0 14 839.0 15 957.5 16 1123.0 17 1285.5 18 1412.5 19 1598.5 20 1854.5 21 2176.0 22 2709.5 23 3481.5 24 4509.5 25 5977.5 26 8107.0 27 11112.5 28 14930.0 29 19924.0 30 26047.0 31 33631.5 32 43048.0 33 54025.0 34 65579.0 35 76079.5 36 87099.5 37 97582.5 38 104636.0 39 109642.0 40 114170.5 41 117836.0 42 120792.0 43 123850.0 44 126319.5 45 127844.5 46 127803.5 47 125782.5 48 123006.0 49 119365.0 50 113368.0 51 106787.0 52 100956.5 53 94580.5 54 88050.5 55 79700.5 56 69980.0 57 60917.0 58 52786.5 59 45255.0 60 37379.0 61 29659.0 62 22857.0 63 17440.5 64 13136.5 65 9673.0 66 7074.5 67 5263.0 68 3971.0 69 3033.5 70 2306.5 71 1733.5 72 1294.0 73 962.0 74 715.5 75 540.0 76 410.0 77 305.5 78 213.5 79 154.0 80 112.0 81 90.0 82 77.5 83 63.0 84 47.0 85 33.0 86 30.0 87 26.0 88 20.0 89 15.0 90 12.5 91 6.0 92 6.0 93 5.5 94 2.0 95 2.0 96 1.5 97 1.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02767000450769528 2 0.29484486015412026 3 0.0 4 0.4330942644943868 5 0.2229363878335158 6 0.1023622469787709 7 0.11292715779080001 8 0.11537553394723848 9 0.11463766716036664 10-11 0.13456007040590723 12-13 0.1844499270182669 14-15 0.13142413656170177 16-17 0.1217312501341576 18-19 0.152369491489042 20-21 0.11680095842187735 22-23 0.10199331358533496 24-25 0.10229516817996437 26-27 0.15820534698521047 28-29 0.11810899499860476 30-31 0.12870744521003713 32-33 0.10678944770000215 34-35 0.151866400497993 36-37 0.14629886019705068 38-39 0.14108348358984266 40-41 0.11768975250606392 42-43 0.11487244295618951 44-45 0.09275320904973491 46-47 0.09967909502650954 48-49 0.10442492004207181 50-51 0.09033837229269968 52-53 0.09897476763904094 54-55 0.3180205851417778 56-57 0.3396199583574817 58-59 0.3518786088393758 60-61 0.16925657908858693 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2981568.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.50284879827078 #Duplication Level Percentage of deduplicated Percentage of total 1 84.55553091359627 62.99627934713173 2 10.257123925212937 15.283699058105302 3 2.6032094373386587 5.818395573008209 4 1.0120846868174658 3.0161276957202756 5 0.47871337553054627 1.783275511743105 6 0.27578417523877785 1.2328024025262285 7 0.17489341214766377 0.9121040208735733 8 0.11504875194463646 0.6857167816448808 9 0.08943096930699948 0.5996575785745959 >10 0.40773310283889996 5.304237522412937 >50 0.022725317327024142 1.1604000485174562 >100 0.007402811983813071 0.9181955659691351 >500 1.823546952550218E-4 0.07995328210733893 >1k 1.3676602101480732E-4 0.20915561166526744 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.3539399403267E-5 2 0.0 0.0 0.0 0.0 3.3539399403267E-5 3 0.0 0.0 0.0 0.0 3.3539399403267E-5 4 0.0 0.0 0.0 3.3539399403267E-5 3.3539399403267E-5 5 0.0 0.0 0.0 3.3539399403267E-5 3.3539399403267E-5 6 0.0 0.0 0.0 3.3539399403267E-5 3.3539399403267E-5 7 0.0 0.0 0.0 6.7078798806534E-5 3.3539399403267E-5 8 0.0 0.0 0.0 6.7078798806534E-5 3.3539399403267E-5 9 0.0 0.0 0.0 1.0061819820980102E-4 3.3539399403267E-5 10-11 0.0 0.0 0.0 1.5092729731470153E-4 3.3539399403267E-5 12-13 0.0 0.0 0.0 1.8446669671796852E-4 3.3539399403267E-5 14-15 0.0 0.0 0.0 2.0123639641960204E-4 6.7078798806534E-5 16-17 0.0 0.0 0.0 2.0123639641960204E-4 1.0061819820980102E-4 18-19 0.0 0.0 0.0 2.3477579582286903E-4 1.0061819820980102E-4 20-21 0.0 0.0 0.0 3.0185459462940306E-4 1.34157597613068E-4 22-23 0.0 0.0 0.0 4.1924249254083755E-4 1.5092729731470153E-4 24-25 0.0 0.0 0.0 6.875576877669737E-4 1.6769699701633503E-4 26-27 0.0 0.0 0.0 0.0011738789791143453 2.0123639641960204E-4 28-29 0.0 0.0 0.0 0.0027502307510678947 2.0123639641960204E-4 30-31 0.0 0.0 0.0 0.008502237748728186 2.0123639641960204E-4 32-33 0.0 0.0 0.0 0.024248985768562048 2.0123639641960204E-4 34-35 0.0 0.0 0.0 0.05002401420997274 2.0123639641960204E-4 36-37 0.0 0.0 0.0 0.09032160259299804 2.3477579582286903E-4 38-39 0.0 0.0 0.0 0.14670133298988988 2.5154549552450255E-4 40-41 1.67696997016335E-5 0.0 0.0 0.20703871251636724 2.68315195226136E-4 42-43 3.3539399403267E-5 0.0 0.0 0.2701430924936141 2.68315195226136E-4 44-45 3.3539399403267E-5 0.0 0.0 0.35070472986026147 3.0185459462940306E-4 46-47 3.3539399403267E-5 0.0 0.0 0.4484217700216799 3.3539399403267005E-4 48-49 3.3539399403267E-5 0.0 0.0 0.5472120709640029 3.3539399403267005E-4 50-51 3.3539399403267E-5 0.0 0.0 0.6490712269517247 4.024727928392041E-4 52-53 3.3539399403267E-5 0.0 0.0 0.7501254373537682 4.360121922424711E-4 54-55 3.3539399403267E-5 0.0 0.0 0.8636227649344238 4.360121922424711E-4 56-57 3.3539399403267E-5 0.0 0.0 0.9977803625474917 4.527818919441046E-4 58-59 3.3539399403267E-5 0.0 0.0 1.1478356354777084 4.6955159164573806E-4 60-61 3.3539399403267E-5 0.0 0.0 1.3044814003906668 4.6955159164573806E-4 62-63 3.3539399403267E-5 0.0 0.0 1.4588800255436065 4.6955159164573806E-4 64-65 3.3539399403267E-5 0.0 0.0 1.622585834030953 4.6955159164573806E-4 66-67 3.3539399403267E-5 0.0 0.0 1.8118822042629918 4.6955159164573806E-4 68-69 3.3539399403267E-5 0.0 0.0 2.0234487356988 4.6955159164573806E-4 70-71 3.3539399403267E-5 0.0 0.0 2.2476428510099384 4.6955159164573806E-4 72-73 3.3539399403267E-5 0.0 0.0 2.481781398244145 4.6955159164573806E-4 74-75 3.3539399403267E-5 0.0 0.0 2.7173453699529913 4.6955159164573806E-4 76-77 3.3539399403267E-5 0.0 0.0 2.9733012964990233 5.030909910490051E-4 78-79 3.3539399403267E-5 0.0 0.0 3.2587215854208256 5.030909910490051E-4 80-81 3.3539399403267E-5 0.0 0.0 3.5639636593899584 5.534000901539056E-4 82-83 3.3539399403267E-5 0.0 0.0 3.8823364082254708 6.037091892588061E-4 84-85 3.3539399403267E-5 0.0 0.0 4.204499109193552 6.037091892588061E-4 86-87 3.3539399403267E-5 0.0 0.0 4.540815436709812 6.037091892588061E-4 88-89 3.3539399403267E-5 3.3539399403267E-5 0.0 4.90473468993496 6.037091892588061E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3000 0.0 41.890446 1 GTATCAA 5100 0.0 37.38378 1 CAACGCA 5290 0.0 34.61211 5 ATCAACG 5265 0.0 34.49463 3 TCAACGC 5575 0.0 33.01185 4 AACGCAG 5740 0.0 32.127182 6 TATCAAC 6045 0.0 30.907812 2 ACGCAGA 6425 0.0 28.042309 7 CGCAGAG 6470 0.0 27.259924 8 GCAGAGT 7845 0.0 21.815472 9 GTACATG 6890 0.0 20.389595 1 TACATGG 6915 0.0 19.625195 2 ACATGGG 6790 0.0 18.954786 3 CAGAGTA 7640 0.0 18.07486 10-11 AGAGTAC 6750 0.0 17.922813 10-11 GAGTACT 4035 0.0 17.84822 12-13 GTACTAG 1035 0.0 17.543802 1 TAGCCCT 2435 0.0 17.035217 4 CTAGTAC 885 0.0 16.697187 3 CATGGGG 4840 0.0 16.254215 4 >>END_MODULE