FastQCFastQC Report
Sat 18 Jun 2016
SRR1931143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981568
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39940.13395636121664842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31170.10454230793998326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35550.043.5980341
GTATCAA58700.036.204151
ATCAACG67150.030.9060423
CAACGCA68250.030.2672335
TCAACGC69650.029.7270264
AACGCAG72850.028.61686
TATCAAC75000.028.0511362
ACGCAGA84150.024.322567
CGCAGAG84300.023.9976188
GTACATG73250.022.067861
TACATGG74900.020.162912
GCAGAGT98550.019.8530259
ACATGGG74300.019.2391383
AGAGTAC85750.019.05067810-11
GAGTACT51850.018.18016812-13
GTACTAG10300.018.0018161
GTGGTAT19800.017.7686921
CAGAGTA94000.017.65653410-11
TGCGACG2850.017.50183322-23
ACGGGCG5000.017.0957685
Produced by FastQC (version 0.11.5)