##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931143_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2981568 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.322771105673258 31.0 31.0 34.0 30.0 34.0 2 31.323563306287163 31.0 31.0 34.0 28.0 34.0 3 31.479204901581987 31.0 31.0 34.0 30.0 34.0 4 35.12601959774186 35.0 35.0 37.0 32.0 37.0 5 34.99039867613283 35.0 35.0 37.0 32.0 37.0 6 35.10705608592526 36.0 35.0 37.0 32.0 37.0 7 34.966138622362465 36.0 35.0 37.0 32.0 37.0 8 35.00106554671904 36.0 35.0 37.0 32.0 37.0 9 36.43450694399725 38.0 35.0 39.0 32.0 39.0 10-11 36.31980789973598 38.0 35.0 39.0 32.0 39.0 12-13 36.23922211400176 38.0 35.0 39.0 32.0 39.0 14-15 37.226271545710176 39.0 36.0 40.5 31.5 41.0 16-17 37.26020050523751 39.0 36.0 40.5 32.0 41.0 18-19 37.27664453737094 39.0 36.0 40.0 31.5 41.0 20-21 37.22885508564621 39.0 36.0 40.0 31.0 41.0 22-23 37.12599544937429 39.0 36.0 40.0 31.0 41.0 24-25 36.986790507545024 39.0 36.0 40.0 31.0 41.0 26-27 36.7438228811149 39.0 36.0 40.0 30.0 41.0 28-29 36.47548789764312 38.0 35.0 40.0 30.0 41.0 30-31 36.277699183785174 38.0 35.0 40.0 30.0 41.0 32-33 36.15968812383283 38.0 35.0 40.0 30.0 41.0 34-35 35.93783338163007 38.0 34.5 40.0 29.0 41.0 36-37 36.23968210686458 38.0 35.0 40.0 30.0 41.0 38-39 36.24151084261704 38.0 35.0 40.0 30.0 41.0 40-41 36.10700208749222 38.0 35.0 40.0 30.0 41.0 42-43 35.974425369470026 38.0 35.0 40.0 29.0 41.0 44-45 35.780837129993344 38.0 34.5 40.0 28.5 41.0 46-47 35.64433160672505 38.0 34.0 40.0 28.0 41.0 48-49 35.44602286447936 38.0 34.0 40.0 27.0 41.0 50-51 35.11101155499388 38.0 34.0 40.0 26.0 41.0 52-53 35.00525595928049 38.0 33.5 40.0 26.0 41.0 54-55 34.78819684810141 38.0 33.0 40.0 26.0 41.0 56-57 34.58235113202181 38.0 33.0 40.0 26.0 41.0 58-59 34.352046305836396 37.0 33.0 40.0 25.5 41.0 60-61 34.07583358823277 37.0 32.5 40.0 25.0 41.0 62-63 33.804499679363346 37.0 32.0 40.0 24.0 41.0 64-65 33.57444623097645 36.0 32.0 39.0 23.0 41.0 66-67 33.324902534505334 36.0 32.0 39.0 23.0 40.5 68-69 33.00884031489471 35.5 31.0 39.0 22.5 40.0 70-71 32.150439131356386 35.0 31.0 38.0 20.0 40.0 72-73 32.115600080226244 35.0 31.0 38.0 22.0 40.0 74-75 31.80435646612789 35.0 31.0 37.0 21.0 39.0 76-77 31.05230720882435 34.0 30.0 36.0 20.0 39.0 78-79 31.06887030582566 34.0 30.0 36.0 20.0 39.0 80-81 30.77526757732844 34.0 30.0 36.0 18.5 37.5 82-83 30.408199812984307 34.0 30.0 35.5 18.0 37.0 84-85 30.049264849904482 34.0 29.5 35.0 16.5 37.0 86-87 29.71054626290596 34.0 29.0 35.0 12.5 36.0 88-89 29.388562494633696 34.0 29.0 35.0 8.5 36.0 90-91 29.08544564470775 34.0 29.0 35.0 4.5 36.0 92-93 28.733982421329983 34.0 29.0 35.0 2.0 35.0 94-95 28.34690421281688 33.0 29.0 35.0 2.0 35.0 96-97 27.978820540064824 33.0 28.5 35.0 2.0 35.0 98-99 27.495620760619914 33.0 27.0 35.0 2.0 35.0 100-101 26.79103746753386 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7874901831635199 1101 2 0.6984079824870371 1101 3 0.49243536039627855 1101 4 0.8089524183789294 1101 5 0.6996968695019916 1101 6 0.8476176718878037 1101 7 0.7084856485437427 1101 8 0.6539155037362434 1101 9 1.0846541574534427 1101 10-11 0.893625145513667 1101 12-13 1.105682609461752 1101 14-15 1.3529571544558365 1101 16-17 1.059460263441018 1101 18-19 0.8817373341363037 1101 20-21 0.7320847198509952 1101 22-23 0.689685101564173 1101 24-25 0.5770359657229918 1101 26-27 0.9253480928312072 1101 28-29 0.8914146460361962 1101 30-31 0.9895919774757544 1101 32-33 1.1653365835890597 1101 34-35 0.812777772808893 1101 36-37 1.2130311131238543 1101 38-39 0.9369899502219283 1101 40-41 0.9630744351598395 1101 42-43 1.027734271429324 1101 44-45 0.8934174313668706 1101 46-47 0.9892808587412532 1101 48-49 0.869047072793613 1101 50-51 0.8363676425745226 1101 52-53 0.6242908118385628 1101 54-55 0.6166557385918026 1101 56-57 0.3994708774102378 1101 58-59 0.5701176122520266 1101 60-61 0.7466088994496971 1101 62-63 0.7359122746714348 1101 64-65 0.6829719251484931 1101 66-67 0.31919932814275853 1101 68-69 0.4389808545904472 1101 70-71 0.774315295010453 1101 72-73 0.2677312539033174 1101 74-75 -0.14218499057116318 1101 76-77 -0.06727911972620149 1101 78-79 0.08490437606187129 1101 80-81 -0.06627654846862896 1101 82-83 -0.16397075474598566 1101 84-85 -0.10750099763496834 1101 86-87 0.008198342921186708 1101 88-89 -0.21539395701517705 1101 90-91 -0.21858247211410387 1101 92-93 -0.3328629413604176 1101 94-95 -0.1903778086635306 1101 96-97 -0.3343543164296179 1101 98-99 -0.3655910124990065 1101 100-101 -0.4675757407427241 1102 1 0.882220809582634 1102 2 0.8424197662994004 1102 3 0.5550490470165599 1102 4 0.8068243794764314 1102 5 0.8502385650535373 1102 6 0.9323357534363694 1102 7 0.9467754443941416 1102 8 0.956997789396226 1102 9 1.3601535663540858 1102 10-11 1.1784234549410826 1102 12-13 1.3433921695140754 1102 14-15 1.6557288642091947 1102 16-17 1.4319813925524727 1102 18-19 1.2822605514874539 1102 20-21 1.1979755863811263 1102 22-23 0.8695578943837461 1102 24-25 0.6924445106114163 1102 26-27 1.0465115818565707 1102 28-29 1.2308876152718113 1102 30-31 1.4732173475981725 1102 32-33 1.5763625257640754 1102 34-35 1.5091764730807071 1102 36-37 1.7219414637994817 1102 38-39 1.40802780779034 1102 40-41 1.4677575421453781 1102 42-43 1.6082738430031185 1102 44-45 1.4908572462437348 1102 46-47 1.5625342322311653 1102 48-49 1.1787632354521378 1102 50-51 1.1408407681641464 1102 52-53 1.051620110832495 1102 54-55 0.8069643489052396 1102 56-57 1.0050332827602233 1102 58-59 1.265615551018918 1102 60-61 1.4883550325309187 1102 62-63 1.4012997828394091 1102 64-65 1.3045237609678537 1102 66-67 0.7477328575480726 1102 68-69 0.8429991930875289 1102 70-71 1.1882074903465565 1102 72-73 0.7452457207097645 1102 74-75 0.6281613355767952 1102 76-77 0.6791767208268276 1102 78-79 0.7137372320078512 1102 80-81 0.6287421754309115 1102 82-83 0.7397307130729089 1102 84-85 0.7073631713475663 1102 86-87 0.6606633957708716 1102 88-89 0.6577595935811829 1102 90-91 0.7002730490706455 1102 92-93 0.60890199481263 1102 94-95 0.6438400416029388 1102 96-97 0.6567737106955676 1102 98-99 0.6207739031246611 1102 100-101 0.5133031799354377 1103 1 0.6483938559340423 1103 2 0.4615193935296418 1103 3 0.3266613720868854 1103 4 0.6396885637281571 1103 5 0.5781454474926448 1103 6 0.6879033605893525 1103 7 0.5868918262891256 1103 8 0.45898329174608676 1103 9 0.9043558613502967 1103 10-11 0.7608374608378696 1103 12-13 0.9231290947186253 1103 14-15 1.1856158890150823 1103 16-17 0.9826628933236421 1103 18-19 0.8178604751227425 1103 20-21 0.8279588399470654 1103 22-23 0.592852330701227 1103 24-25 0.35651886261053534 1103 26-27 0.8366832498692602 1103 28-29 0.6646797792174368 1103 30-31 0.9812665064722879 1103 32-33 1.0803013103407295 1103 34-35 0.8221567762469704 1103 36-37 1.0572746028083415 1103 38-39 0.8099445337279789 1103 40-41 0.9802291889389494 1103 42-43 1.1245899226111078 1103 44-45 0.9622781476483482 1103 46-47 1.0167629878779678 1103 48-49 0.826282817959644 1103 50-51 0.7430413423448172 1103 52-53 0.2951515447243267 1103 54-55 0.2599252778404946 1103 56-57 0.5233785275016771 1103 58-59 0.8707155347783768 1103 60-61 0.9410035969086294 1103 62-63 0.687554547854333 1103 64-65 0.5922413797943591 1103 66-67 0.4621776004385154 1103 68-69 0.38195934432336287 1103 70-71 0.8500866582603734 1103 72-73 0.23533010330495685 1103 74-75 0.019011352610295518 1103 76-77 0.22338825025082798 1103 78-79 0.23989565584359696 1103 80-81 0.15760934802377236 1103 82-83 0.0637319545012005 1103 84-85 0.10470654692037584 1103 86-87 0.12378264696316776 1103 88-89 -0.006039632694214703 1103 90-91 -0.12342456035682403 1103 92-93 -0.32497358010578026 1103 94-95 -0.39139356633416966 1103 96-97 -0.5013112616985147 1103 98-99 -0.6443023039907096 1103 100-101 -0.7305960752678082 1104 1 0.44733911045420527 1104 2 0.4142805145608186 1104 3 0.20275442349377926 1104 4 0.6115429010866507 1104 5 0.4146878600678079 1104 6 0.6168038338688433 1104 7 0.49783583531989706 1104 8 0.3966701030048725 1104 9 0.8742411655150661 1104 10-11 0.6307231376126339 1104 12-13 0.8300501956871855 1104 14-15 1.1015767675581571 1104 16-17 0.875022233862282 1104 18-19 0.7352877197820362 1104 20-21 0.6801068393392242 1104 22-23 0.32108735493869034 1104 24-25 0.331183910529802 1104 26-27 0.1586918153629142 1104 28-29 0.5824961583101924 1104 30-31 0.8292685555032264 1104 32-33 1.0235463772274613 1104 34-35 0.8346114433815046 1104 36-37 0.9859648916523582 1104 38-39 0.530343812873447 1104 40-41 0.8275653677165735 1104 42-43 1.1216908386808626 1104 44-45 1.0229841722982798 1104 46-47 0.9316168421395119 1104 48-49 0.7298660300327313 1104 50-51 0.6351874689667554 1104 52-53 0.3781522507392552 1104 54-55 0.7059472935827813 1104 56-57 0.6942406206561174 1104 58-59 0.6937658530035051 1104 60-61 0.8783391827503522 1104 62-63 0.013344051722512518 1104 64-65 -0.9472235425391347 1104 66-67 -0.30441977697037004 1104 68-69 0.3649334654255014 1104 70-71 0.9941768532066817 1104 72-73 0.37973946300870765 1104 74-75 0.12039767238029953 1104 76-77 -0.06919246922342381 1104 78-79 -0.06400783881690231 1104 80-81 -0.033408402144146976 1104 82-83 -0.1278248327193765 1104 84-85 -0.2756994975264888 1104 86-87 0.006363518721499872 1104 88-89 0.09792846929853383 1104 90-91 0.05239253285107992 1104 92-93 0.05223373717680957 1104 94-95 0.18925598684164768 1104 96-97 0.2555975917416653 1104 98-99 0.1362051389748551 1104 100-101 -0.34366619690391076 1105 1 -0.0013763301687461649 1105 2 -1.5505884691217453 1105 3 -0.22409164351253352 1105 4 0.5085527761799824 1105 5 0.6102367855515638 1105 6 0.30257997899995814 1105 7 -0.024002568434099203 1105 8 0.03201044697476618 1105 9 0.801834188812478 1105 10-11 1.0031658625312616 1105 12-13 1.0668270864458478 1105 14-15 1.2846917786914673 1105 16-17 0.9411774122941736 1105 18-19 0.7554621066835168 1105 20-21 1.1171142409319756 1105 22-23 0.40306263935293174 1105 24-25 0.028649032382922712 1105 26-27 1.030280671527322 1105 28-29 0.5835760406247203 1105 30-31 0.9281953935466802 1105 32-33 1.1208988372617767 1105 34-35 0.7539183930493465 1105 36-37 1.000177758863714 1105 38-39 0.8992243392180157 1105 40-41 1.1321174264714102 1105 42-43 1.403297934356786 1105 44-45 1.3768533098080482 1105 46-47 1.475462757362834 1105 48-49 1.1841916663841872 1105 50-51 0.8777482882065044 1105 52-53 0.9441875870459029 1105 54-55 0.16956779828933577 1105 56-57 0.46924099117014606 1105 58-59 -0.03203457229550111 1105 60-61 0.5916285245692308 1105 62-63 1.0289986018137043 1105 64-65 1.5535052968933698 1105 66-67 1.2490461901658065 1105 68-69 1.2559166359115252 1105 70-71 0.6106893604828088 1105 72-73 -0.0656931977266666 1105 74-75 -0.03504218910366674 1105 76-77 0.3813935306610823 1105 78-79 -0.19768378264390662 1105 80-81 0.008982721668356675 1105 82-83 0.06188468938906766 1105 84-85 0.16559332048059616 1105 86-87 -0.24459023550555514 1105 88-89 -0.5645957482652655 1105 90-91 -0.6072320163355514 1105 92-93 -0.30082944287614666 1105 94-95 -0.6713255106585336 1105 96-97 -0.7809536942997681 1105 98-99 -1.2615255775584053 1105 100-101 -0.7296138005980133 1106 1 0.3996450293223788 1106 2 0.4009247112347083 1106 3 -0.2854742477635419 1106 4 0.3992562422122887 1106 5 0.03637289473042671 1106 6 0.5848388670099922 1106 7 0.578468980766857 1106 8 0.4727930805712788 1106 9 1.0991826784985577 1106 10-11 0.26608411034972335 1106 12-13 0.4260989435190865 1106 14-15 0.23364420975579492 1106 16-17 -0.21210963802653993 1106 18-19 0.5194337556421331 1106 20-21 0.5257705662912286 1106 22-23 0.41806607765743564 1106 24-25 0.37514126646916424 1106 26-27 0.8559108405136797 1106 28-29 0.8107121387351697 1106 30-31 0.41470506464724366 1106 32-33 0.5291563874123497 1106 34-35 -0.9626710359310096 1106 36-37 -0.6584807659887133 1106 38-39 -0.6661012326005391 1106 40-41 0.5963120898482046 1106 42-43 0.8763676520468806 1106 44-45 0.6170639616555249 1106 46-47 0.24894492969013982 1106 48-49 -0.041934367696889296 1106 50-51 0.5654598475222983 1106 52-53 -0.02652912703048571 1106 54-55 0.5919724318126214 1106 56-57 0.28638483433464046 1106 58-59 0.641315573679897 1106 60-61 0.8952715096475785 1106 62-63 0.6898882074062698 1106 64-65 0.805732759112459 1106 66-67 0.44705282497245946 1106 68-69 0.6273972410644646 1106 70-71 0.9443017030735916 1106 72-73 0.24731188040642493 1106 74-75 0.35166052654670565 1106 76-77 0.46375572389918673 1106 78-79 0.5122271903863336 1106 80-81 0.4482039421515154 1106 82-83 0.39502707833381834 1106 84-85 0.5212275847813466 1106 86-87 0.5035564999215261 1106 88-89 0.481247990089841 1106 90-91 0.6947489465543164 1106 92-93 0.2546458903044986 1106 94-95 0.533203855981089 1106 96-97 0.7976044258175357 1106 98-99 0.8003099520043442 1106 100-101 0.6021237223866898 1107 1 0.2927632184489042 1107 2 0.3598186011285627 1107 3 0.0728567582736126 1107 4 -1.4280052892066806 1107 5 -0.1004042090079551 1107 6 0.34625075793146465 1107 7 0.49404511490577363 1107 8 0.4274926568542341 1107 9 0.9603613060547218 1107 10-11 0.7387378072655864 1107 12-13 0.7695810856613932 1107 14-15 0.9736899676168349 1107 16-17 0.8539342473226128 1107 18-19 0.5942383451800879 1107 20-21 0.10896877516013603 1107 22-23 0.31032668365239147 1107 24-25 0.3518212407174204 1107 26-27 0.6939826430559748 1107 28-29 0.6370784107269429 1107 30-31 0.8868466465147762 1107 32-33 1.0266294382645214 1107 34-35 0.7307265464752959 1107 36-37 1.0226776953302092 1107 38-39 0.8141017814089153 1107 40-41 0.3323140508993987 1107 42-43 0.21915935252409469 1107 44-45 -0.25126616840775995 1107 46-47 0.15692627615618449 1107 48-49 0.43219678160438235 1107 50-51 -0.9142394579622888 1107 52-53 0.3489621600906716 1107 54-55 0.7959488116009794 1107 56-57 0.6556344072973843 1107 58-59 0.8812209166966696 1107 60-61 1.2113260150025553 1107 62-63 1.0686457938816076 1107 64-65 1.020757170997257 1107 66-67 0.6953331846492148 1107 68-69 0.7761999590324322 1107 70-71 1.131505726336826 1107 72-73 0.8273422663388494 1107 74-75 0.5689364738582903 1107 76-77 0.5647929052224789 1107 78-79 0.7134667640602075 1107 80-81 0.7848047208263544 1107 82-83 0.8611314576851363 1107 84-85 0.6478564134582818 1107 86-87 0.6217553751899096 1107 88-89 0.7765394656866711 1107 90-91 0.9519123641899299 1107 92-93 0.8865367081673945 1107 94-95 0.9304840041996343 1107 96-97 0.9091558603189434 1107 98-99 0.8633176224271821 1107 100-101 0.7715568966475637 1108 1 0.5447648012714481 1108 2 0.4984700227940664 1108 3 0.28964764193728243 1108 4 0.6060870624008885 1108 5 0.40470941262304905 1108 6 0.6349358314864944 1108 7 0.651796492265774 1108 8 0.6341055631712109 1108 9 0.8794047806884109 1108 10-11 0.8023598892183657 1108 12-13 0.9528939760824997 1108 14-15 1.1020365268379635 1108 16-17 0.846805739944557 1108 18-19 0.6551599898274105 1108 20-21 0.5439546774422652 1108 22-23 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1.4760367439477555 2311 84-85 1.6679114851759707 2311 86-87 1.6833327400633742 2311 88-89 1.8264172726612884 2311 90-91 1.7907406122757124 2311 92-93 1.5833424353997536 2311 94-95 1.9562025246457502 2311 96-97 1.942225054948107 2311 98-99 1.847586684680568 2311 100-101 2.2425204961372955 2312 1 -0.10755741398062568 2312 2 -0.15719081560250103 2312 3 -0.2976529016752245 2312 4 0.24541144748255306 2312 5 -0.9399462491038904 2312 6 0.10735233925149856 2312 7 0.379937991456309 2312 8 -2.6745168882564165 2312 9 -5.304833122200609 2312 10-11 -0.5257442021810448 2312 12-13 -5.656996075222153 2312 14-15 -1.9034407379327831 2312 16-17 -2.746251516189517 2312 18-19 -2.211919021469569 2312 20-21 -3.5076734055905945 2312 22-23 -2.3106477807672903 2312 24-25 -2.228657463921941 2312 26-27 -2.5470486354887 2312 28-29 -6.214499567917027 2312 30-31 -7.008789010090567 2312 32-33 -1.8773165986926301 2312 34-35 0.46137833494371705 2312 36-37 1.1586942911184437 2312 38-39 1.0233759056051 2312 40-41 -2.141853161233989 2312 42-43 -4.604699273757991 2312 44-45 -2.929445875920358 2312 46-47 0.5616549010419121 2312 48-49 -1.0819320960308758 2312 50-51 -6.927150927746837 2312 52-53 -1.4651865724158455 2312 54-55 0.8358913080951638 2312 56-57 1.218120008558813 2312 58-59 1.5968981929108068 2312 60-61 1.9686879691298955 2312 62-63 1.8157965583029423 2312 64-65 1.6629850528641157 2312 66-67 1.1203708455941381 2312 68-69 1.6325753775411087 2312 70-71 1.916154499115848 2312 72-73 1.2273489842846637 2312 74-75 1.0726709131085421 2312 76-77 1.2459446003079329 2312 78-79 1.2335631824102649 2312 80-81 1.3410974136357936 2312 82-83 0.6688372932586653 2312 84-85 0.9161112881017637 2312 86-87 1.249595597817713 2312 88-89 1.3374124082278307 2312 90-91 1.5604669823204347 2312 92-93 1.6516384569084828 2312 94-95 1.8168327162357691 2312 96-97 1.8872872760011639 2312 98-99 2.0888153219703973 2312 100-101 1.992985314824569 2313 1 -0.14721767925518492 2313 2 -0.05836335862165498 2313 3 -0.29657282017816655 2313 4 -0.9829685120962139 2313 5 -1.7089888223162362 2313 6 -2.1666692460135692 2313 7 -1.4694970113666344 2313 8 -4.299139473415117 2313 9 -2.1942556877214585 2313 10-11 -1.2859783834874463 2313 12-13 -3.595022308110636 2313 14-15 -2.0549712625087295 2313 16-17 -4.960619054831074 2313 18-19 -3.0495713170518712 2313 20-21 -4.067548377974873 2313 22-23 -2.588281911342598 2313 24-25 -0.8045372803080895 2313 26-27 -3.5835586630635063 2313 28-29 -3.6914823766198666 2313 30-31 -2.3568166339930983 2313 32-33 -8.459627809447621 2313 34-35 -2.300655982191124 2313 36-37 -6.058774390609361 2313 38-39 -2.656981339532493 2313 40-41 -2.312006909198608 2313 42-43 -2.3088919537511146 2313 44-45 -3.7656253330975815 2313 46-47 -1.5978739543171798 2313 48-49 -1.1491917165295078 2313 50-51 0.26790197169921726 2313 52-53 0.23492716343876197 2313 54-55 -4.441787825875853 2313 56-57 -5.861286437563944 2313 58-59 -1.403056803693481 2313 60-61 -8.993521452126437 2313 62-63 -1.9378998569720238 2313 64-65 -3.900780667640248 2313 66-67 -3.751430844897051 2313 68-69 -5.545061207764867 2313 70-71 -6.888641226379804 2313 72-73 -1.9513799561717526 2313 74-75 0.9743671320050602 2313 76-77 -0.39555425824230284 2313 78-79 0.7936795366442624 2313 80-81 1.610369095044483 2313 82-83 1.3398256496528305 2313 84-85 0.9296736750818724 2313 86-87 0.9293023393263873 2313 88-89 -3.9102412783685203 2313 90-91 0.3034075860205334 2313 92-93 1.3842241883773134 2313 94-95 1.6401862056354197 2313 96-97 1.7261955754455762 2313 98-99 2.2883644697697285 2313 100-101 2.3619190616436434 2314 1 -28.795569327606835 2314 2 -14.488082892818802 2314 3 -4.753026813945592 2314 4 -2.4733736293670034 2314 5 -0.7444160816240881 2314 6 -0.6196789229038444 2314 7 0.2869405808918586 2314 8 -4.4301875298363065 2314 9 -2.714019505992745 2314 10-11 0.39575033555470185 2314 12-13 1.037583891659743 2314 14-15 -0.08744625020759855 2314 16-17 -0.5152360795973507 2314 18-19 -3.3696467639929892 2314 20-21 -2.331530664345223 2314 22-23 -5.55998930842313 2314 24-25 -0.5882833849497118 2314 26-27 -2.330647395226869 2314 28-29 -2.0469030753241455 2314 30-31 -4.354356407652276 2314 32-33 -3.0328605468594603 2314 34-35 -1.587026328262965 2314 36-37 -5.356297762759596 2314 38-39 -4.2619509967409535 2314 40-41 -6.640775155877304 2314 42-43 -5.6952294968050765 2314 44-45 -4.4021678950132745 2314 46-47 -6.439845415691622 2314 48-49 -5.149929784431755 2314 50-51 -1.5814501686976996 2314 52-53 -1.5846562382341816 2314 54-55 -4.031914117494679 2314 56-57 -1.6757853714658637 2314 58-59 -0.9264289539312998 2314 60-61 0.4630145491856794 2314 62-63 1.6612472645631513 2314 64-65 0.48137449063431603 2314 66-67 1.5164878036186096 2314 68-69 2.4234478950725915 2314 70-71 2.7471632676336526 2314 72-73 2.1224268340529484 2314 74-75 2.1355119573289905 2314 76-77 -0.3292101545847679 2314 78-79 -1.2651198729014155 2314 80-81 -0.6997834389886464 2314 82-83 -0.6393444339948644 2314 84-85 1.196198195337736 2314 86-87 2.094629549429719 2314 88-89 1.9380447639473708 2314 90-91 2.118562580280006 2314 92-93 1.4678718839653087 2314 94-95 2.169134868912444 2314 96-97 2.5981817981780644 2314 98-99 3.0320088850559372 2314 100-101 2.9782877675645913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 21.0 4 54.0 5 104.0 6 232.0 7 626.0 8 1582.0 9 2902.0 10 4280.0 11 6126.0 12 8040.0 13 10136.0 14 12205.0 15 14184.0 16 15982.0 17 17775.0 18 16942.0 19 15901.0 20 16544.0 21 17899.0 22 19841.0 23 23248.0 24 27952.0 25 34577.0 26 44189.0 27 56014.0 28 71275.0 29 88695.0 30 109804.0 31 133633.0 32 161275.0 33 196302.0 34 239485.0 35 294743.0 36 372334.0 37 462299.0 38 405219.0 39 79112.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.03726808173461 21.11800887718992 15.04576251838223 23.798960522693243 2 14.797046386330951 21.742720608753515 40.30865638482838 23.15157662008715 3 18.25140904498882 26.072967131451385 30.920672209379855 24.754951614179937 4 12.791864927039848 18.024429501478924 38.93255172881033 30.251153842670895 5 12.426343113556959 38.41796293954744 36.22401151611522 12.931682430780386 6 30.084371713138857 38.81762884495675 18.063146639620495 13.0348528022839 7 26.144330768240064 32.96986686200013 22.937259857900273 17.94854251185953 8 24.545474059286924 36.70501561594436 20.747304773863952 18.00220555090476 9 24.997451005645352 16.450236922317384 21.050064932277245 37.50224713976002 10-11 23.734860315109366 27.107699706999806 29.3990443954322 19.758395582458625 12-13 24.499994633696094 24.44341366690278 29.148773397085026 21.907818302316095 14-15 21.76341441818533 26.2330089402623 26.645677710520104 25.357898931032263 16-17 21.624578074355508 29.242516018417152 27.21292957262756 21.91997633459978 18-19 21.5013040118488 28.4894726533153 28.823457992573037 21.18576534226286 20-21 22.292196589177237 27.679395539528194 28.714304017215103 21.314103854079463 22-23 22.06381004894069 27.641361860604892 28.40892778564836 21.88590030480606 24-25 21.986878241987682 27.657570287584416 28.521121917690117 21.834429552737785 26-27 22.071236457024654 27.787759452976246 28.343088317448466 21.79791577255063 28-29 21.948585315509415 27.737713968607956 28.489418397145393 21.824282318737236 30-31 22.233368684500558 27.582429597848613 28.403774055731418 21.780427661919415 32-33 22.17723238429837 27.684525182057918 28.196509351299454 21.94173308234426 34-35 22.35210366515765 27.64411470985042 28.308490041581102 21.695291583410825 36-37 22.236117006192615 27.522908086423282 28.270694167718 21.9702807396661 38-39 22.102631181767872 27.568211165333466 28.36871761332193 21.96044003957673 40-41 22.29666989489395 27.466730392711753 28.327266781541248 21.909332930853047 42-43 22.263437051824365 27.439215812542006 28.245328240649435 22.05201889498419 44-45 22.295852828226927 27.55576797303416 28.091781120558103 22.056598078180812 46-47 22.33217961387275 27.414964423022564 27.945939299471473 22.30691666363321 48-49 22.24560500734134 27.505842714494793 28.051973406801533 22.19657887136233 50-51 22.22874049933136 27.59497227662046 27.961360996118074 22.214926227930107 52-53 22.325681546966216 27.529409436644187 27.878089944545177 22.266819071844417 54-55 22.315257192735054 27.436967367524147 27.95021709184996 22.297558347890842 56-57 22.346425395336873 27.558464343672117 27.771122938334376 22.323987322656635 58-59 22.270649794418528 27.56658213549159 27.96055599399371 22.20221207609617 60-61 22.38678891525176 27.539975300741315 27.819854393778094 22.25338139022883 62-63 22.33513289788701 27.469912412098495 27.772851037511124 22.42210365250337 64-65 22.1965560477468 27.484699247893317 27.798362150336807 22.520382554023076 66-67 22.254994216591783 27.529895197632904 27.69528876539622 22.519821820379097 68-69 22.270758908581946 27.463819131377416 27.744384582282823 22.521037377757818 70-71 22.260321642889654 27.230082300786112 27.700082612722092 22.809513443602143 72-73 22.255068417430177 27.353102766347664 27.673170090333276 22.718658725888876 74-75 22.204125631407887 27.432646611313682 27.60269344798135 22.760534309297086 76-77 22.105885225492088 27.485839665571945 27.536064916178333 22.872210192757635 78-79 22.25803671088501 27.435798881662265 27.41430012664477 22.89186428080795 80-81 22.189088983317315 27.469135750710745 27.435914936604593 22.90586032936735 82-83 22.287130418071204 27.341914630974408 27.600587812795407 22.77036713815898 84-85 22.35062222293773 27.331474579818405 27.394142947603406 22.923760249640456 86-87 22.111730204049117 27.377446849543645 27.530825378058953 22.979997568348278 88-89 22.218732097504954 27.253182032234218 27.460062761871157 23.06802310838967 90-91 22.222287438716293 27.255504528307267 27.433987165952967 23.088220867023477 92-93 22.27663262513349 27.13234741683258 27.463142169463513 23.127877788570416 94-95 22.08260761800693 27.247973447287826 27.4666187719706 23.20280016273464 96-97 22.038432965348616 27.180799951703055 27.512304820806595 23.268462262141735 98-99 22.041351599276204 27.16398261951598 27.3931957182698 23.401470062938014 100-101 22.036120509923983 27.143426853384778 27.331740379893905 23.48871225679733 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 162.0 1 111.5 2 69.5 3 89.5 4 90.5 5 88.0 6 149.5 7 302.0 8 504.5 9 668.0 10 651.0 11 617.5 12 687.5 13 781.0 14 890.5 15 1038.0 16 1166.5 17 1277.5 18 1460.5 19 1668.5 20 1981.0 21 2394.0 22 2885.5 23 3723.5 24 4867.0 25 6525.5 26 9135.5 27 12581.5 28 16677.5 29 22205.0 30 29017.5 31 36983.5 32 47031.0 33 57819.0 34 68153.0 35 77650.5 36 88078.5 37 97908.0 38 104054.0 39 108413.0 40 113059.5 41 116809.0 42 119436.5 43 122663.5 44 125114.5 45 126794.0 46 126613.5 47 124623.0 48 121908.0 49 118329.0 50 112645.5 51 105646.5 52 99643.0 53 93363.0 54 86505.0 55 78392.0 56 69339.0 57 60028.5 58 51936.0 59 44909.5 60 36958.0 61 29009.0 62 22277.5 63 16905.0 64 12625.0 65 9184.0 66 6647.0 67 4953.0 68 3742.0 69 2862.0 70 2153.0 71 1574.0 72 1156.0 73 884.0 74 663.0 75 489.0 76 368.0 77 254.0 78 158.0 79 110.5 80 85.0 81 65.5 82 48.0 83 39.5 84 37.0 85 26.0 86 15.5 87 14.0 88 12.5 89 8.5 90 4.0 91 2.5 92 2.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10558202932148453 2 0.0 3 0.0038905703307789727 4 3.0185459462940306E-4 5 2.3477579582286903E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.018949760662845858 26-27 0.009105946937986992 28-29 0.004812903814368815 30-31 0.0018111275677764182 32-33 0.0010229516817996437 34-35 0.0015260426728486486 36-37 0.0011235698800094447 38-39 6.037091892588061E-4 40-41 0.001861436666881319 42-43 0.0012074183785176123 44-45 6.037091892588061E-4 46-47 0.030805938351900746 48-49 0.016937396698649836 50-51 0.09271966965033164 52-53 0.0014086547749372143 54-55 0.03848646081524889 56-57 9.558728829931097E-4 58-59 8.384849850816751E-4 60-61 0.0034210187391332343 62-63 0.0035551763367463025 64-65 0.011470474595917317 66-67 0.0065234131839354325 68-69 0.0029850065468907637 70-71 0.00627186768841093 72-73 0.005500461502135789 74-75 0.0012241880782192457 76-77 0.0 78-79 0.0 80-81 1.1738789791143452E-4 82-83 2.180060961212355E-4 84-85 0.0 86-87 0.001861436666881319 88-89 0.00469551591645738 90-91 0.0015260426728486486 92-93 0.0037564127331659045 94-95 9.055637838882091E-4 96-97 4.36012192242471E-4 98-99 0.0021129821624058217 100-101 0.003488097537939768 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2981568.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.17174585232912 #Duplication Level Percentage of deduplicated Percentage of total 1 81.78233390762792 57.38809150176385 2 11.778566840432052 16.530451976629383 3 3.2233450791605964 6.785632550676393 4 1.223713731753137 3.4348051592234556 5 0.625192489218715 2.193542423112034 6 0.3369070171889894 1.4184812151631228 7 0.21414733477460982 1.0518964645530295 8 0.14841722463592338 0.8331756613808046 9 0.10238662022628187 0.6466183103877833 >10 0.5219098265519775 6.373168564879503 >50 0.030392750991440648 1.4790016913570185 >100 0.01230298945330149 1.442280344152523 >500 2.4011749067379443E-4 0.11592430908681821 >1k 1.4407049438877866E-4 0.30692982763420834 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3994 0.13395636121664842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3117 0.10454230793998326 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.3539399403267E-5 0.0 2 0.0 0.0 0.0 3.3539399403267E-5 0.0 3 0.0 0.0 0.0 6.7078798806534E-5 0.0 4 0.0 0.0 0.0 6.7078798806534E-5 0.0 5 0.0 0.0 0.0 1.0061819820980102E-4 0.0 6 0.0 0.0 0.0 1.0061819820980102E-4 0.0 7 0.0 0.0 0.0 1.0061819820980102E-4 0.0 8 0.0 0.0 0.0 1.0061819820980102E-4 0.0 9 0.0 0.0 0.0 1.34157597613068E-4 0.0 10-11 0.0 0.0 0.0 1.8446669671796852E-4 0.0 12-13 0.0 1.67696997016335E-5 0.0 2.1800609612123554E-4 0.0 14-15 0.0 3.3539399403267E-5 0.0 2.3477579582286903E-4 0.0 16-17 0.0 3.3539399403267E-5 0.0 2.3477579582286903E-4 0.0 18-19 0.0 3.3539399403267E-5 0.0 2.3477579582286903E-4 0.0 20-21 0.0 3.3539399403267E-5 0.0 3.0185459462940306E-4 0.0 22-23 0.0 3.3539399403267E-5 0.0 4.360121922424711E-4 0.0 24-25 0.0 3.3539399403267E-5 0.0 7.546364865735075E-4 3.3539399403267E-5 26-27 0.0 3.3539399403267E-5 0.0 0.0012744971773241461 3.3539399403267E-5 28-29 0.0 5.0309099104900504E-5 0.0 0.002699921651962994 5.0309099104900504E-5 30-31 0.0 6.7078798806534E-5 0.0 0.008686704445446156 6.7078798806534E-5 32-33 0.0 6.7078798806534E-5 0.0 0.025624101144095992 6.7078798806534E-5 34-35 0.0 6.7078798806534E-5 0.0 0.05317671775387984 6.7078798806534E-5 36-37 0.0 8.384849850816751E-5 0.0 0.09666054908021551 6.7078798806534E-5 38-39 0.0 1.0061819820980102E-4 0.0 0.156075595123103 6.7078798806534E-5 40-41 0.0 1.0061819820980102E-4 0.0 0.2192470538991564 6.7078798806534E-5 42-43 0.0 1.0061819820980102E-4 0.0 0.28478304033314017 6.7078798806534E-5 44-45 0.0 1.0061819820980102E-4 0.0 0.3696041814240024 6.7078798806534E-5 46-47 0.0 1.0061819820980102E-4 0.0 0.47179873140575695 1.0061819820980102E-4 48-49 0.0 1.0061819820980102E-4 0.0 0.575754099856183 1.5092729731470153E-4 50-51 0.0 1.0061819820980102E-4 0.0 0.681453517075579 1.6769699701633503E-4 52-53 0.0 1.0061819820980102E-4 0.0 0.7849896430334643 1.6769699701633503E-4 54-55 0.0 1.0061819820980102E-4 0.0 0.9014887468607122 1.6769699701633503E-4 56-57 0.0 1.0061819820980102E-4 0.0 1.0397549209006804 1.8446669671796852E-4 58-59 0.0 1.0061819820980102E-4 0.0 1.1926275033807716 2.0123639641960204E-4 60-61 0.0 1.0061819820980102E-4 0.0 1.3471770558310259 2.0123639641960204E-4 62-63 0.0 1.0061819820980102E-4 0.0 1.4993453109236483 2.68315195226136E-4 64-65 0.0 1.0061819820980102E-4 0.0 1.661290300942323 2.68315195226136E-4 66-67 0.0 1.0061819820980102E-4 0.0 1.8489600103033035 2.68315195226136E-4 68-69 0.0 1.0061819820980102E-4 0.0 2.057910468585657 2.68315195226136E-4 70-71 0.0 1.0061819820980102E-4 0.0 2.280058680533196 2.68315195226136E-4 72-73 0.0 1.0061819820980102E-4 0.0 2.520100162062378 3.0185459462940306E-4 74-75 0.0 1.0061819820980102E-4 0.0 2.7594708556034946 3.0185459462940306E-4 76-77 0.0 1.0061819820980102E-4 0.0 3.0195521282761284 3.0185459462940306E-4 78-79 0.0 1.0061819820980102E-4 0.0 3.309416387618864 3.0185459462940306E-4 80-81 0.0 1.0061819820980102E-4 0.0 3.618113019726533 3.0185459462940306E-4 82-83 0.0 1.0061819820980102E-4 0.0 3.9400744842981945 3.0185459462940306E-4 84-85 0.0 1.0061819820980102E-4 0.0 4.264987416017344 3.0185459462940306E-4 86-87 0.0 1.0061819820980102E-4 0.0 4.603886277287655 3.0185459462940306E-4 88-89 0.0 1.0061819820980102E-4 0.0 4.9711762401528325 3.0185459462940306E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3555 0.0 43.598034 1 GTATCAA 5870 0.0 36.20415 1 ATCAACG 6715 0.0 30.906042 3 CAACGCA 6825 0.0 30.267233 5 TCAACGC 6965 0.0 29.727026 4 AACGCAG 7285 0.0 28.6168 6 TATCAAC 7500 0.0 28.051136 2 ACGCAGA 8415 0.0 24.32256 7 CGCAGAG 8430 0.0 23.997618 8 GTACATG 7325 0.0 22.06786 1 TACATGG 7490 0.0 20.16291 2 GCAGAGT 9855 0.0 19.853025 9 ACATGGG 7430 0.0 19.239138 3 AGAGTAC 8575 0.0 19.050678 10-11 GAGTACT 5185 0.0 18.180168 12-13 GTACTAG 1030 0.0 18.001816 1 GTGGTAT 1980 0.0 17.768692 1 CAGAGTA 9400 0.0 17.656534 10-11 TGCGACG 285 0.0 17.501833 22-23 ACGGGCG 500 0.0 17.095768 5 >>END_MODULE