FastQCFastQC Report
Sat 18 Jun 2016
SRR1931142_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931142_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586255
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.046.356131
GTATCAA46150.041.2233051
ATCAACG52600.035.4800383
TCAACGC52700.035.0568054
CAACGCA53000.034.8076975
TATCAAC56200.032.918912
AACGCAG57150.032.3394856
ACGCAGA62850.029.1098047
CGCAGAG66300.027.3078128
GTACATG64600.023.3236311
TACATGG64900.022.5550482
GCAGAGT75050.022.1616069
ACATGGG67400.020.1311573
GTGGTAT12850.020.0369781
TATACTG12700.019.46795
AGAGTAC64350.019.1997710-11
CAGAGTA71400.019.00111810-11
GAGTACT34800.018.16010712-13
TGGTATC15300.017.1404152
GTATAGA10500.016.8017311
Produced by FastQC (version 0.11.5)