FastQCFastQC Report
Sat 18 Jun 2016
SRR1931142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586255
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28890.11170592226984578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28900.045.555941
GTATCAA49000.036.56861
TCAACGC56200.031.0942274
ATCAACG56350.031.0126573
CAACGCA56750.030.876555
AACGCAG60200.029.1859266
TATCAAC61900.028.3087562
ACGCAGA72050.024.188027
CGCAGAG75250.023.0332188
GTGGTAT16300.022.744171
GTACATG71150.022.579011
TACATGG72900.021.3664022
ACATGGG74150.020.4938663
GTATACG3800.020.0124111
TGGTATC16750.019.2787632
GCAGAGT86200.018.8953579
TAGACGG2101.962204E-518.0900175
AGAGTAC73600.017.6783210-11
GAGTACT39100.017.61000812-13
CATGGGG50850.016.9960784
Produced by FastQC (version 0.11.5)