FastQCFastQC Report
Sat 18 Jun 2016
SRR1931141_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931141_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645184
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56420.2132932907502843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.15654865597251458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28100.053.349591
GTATCAA55050.042.071
ATCAACG66000.034.0745623
CAACGCA67100.033.4192285
TCAACGC68800.032.9079134
TATCAAC71400.032.2362942
AACGCAG71100.031.8485136
ACGCAGA79450.027.7360677
CGCAGAG80100.027.2722828
GTACATG56100.024.4098321
GCAGAGT88150.023.2160059
TACATGG60250.021.2144782
GAGTACT56200.021.12923612-13
ACATGGG59000.020.7176863
AGAGTAC80150.020.48520910-11
CAGAGTA88450.019.34309410-11
GTACTTT64750.018.46885314-15
TAGTACT12350.017.4042784
AGTACTT64850.017.21006212-13
TGGTATC15400.016.849262
Produced by FastQC (version 0.11.5)