##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931141_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2645184 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.544527337228715 31.0 28.0 31.0 22.0 34.0 2 28.21378777430984 31.0 28.0 33.0 16.0 34.0 3 28.28907138407007 31.0 28.0 33.0 16.0 34.0 4 31.450447681522345 35.0 32.0 37.0 17.0 37.0 5 31.54971374392103 35.0 32.0 37.0 17.0 37.0 6 31.82549266894099 35.0 32.0 37.0 19.0 37.0 7 31.46281166073891 35.0 32.0 37.0 17.0 37.0 8 31.64716254143379 35.0 32.0 37.0 17.0 37.0 9 32.624785270136215 37.0 32.0 39.0 17.0 39.0 10-11 32.642544337180325 37.0 31.5 39.0 17.0 39.0 12-13 32.688957554559536 37.0 32.0 39.0 17.0 39.0 14-15 33.415150515049234 38.0 32.0 40.0 17.0 41.0 16-17 33.3941060432847 38.0 32.0 40.0 17.0 41.0 18-19 33.42841027316058 38.0 31.5 40.0 17.5 41.0 20-21 33.69718420344293 38.0 32.0 40.0 19.0 41.0 22-23 33.57605501167404 38.0 32.0 40.0 18.0 41.0 24-25 33.46377945730808 38.0 31.5 40.0 18.5 41.0 26-27 33.08948564636714 37.0 31.0 40.0 16.5 41.0 28-29 33.04819456793932 37.0 31.0 40.0 17.0 41.0 30-31 32.77855075488132 37.0 30.5 40.0 15.5 41.0 32-33 32.5906912335777 37.0 30.0 40.0 16.0 41.0 34-35 32.57548813239457 37.0 30.0 40.0 16.0 41.0 36-37 32.19273479652077 36.5 30.0 40.0 11.0 41.0 38-39 32.02314961832523 36.0 30.0 40.0 11.0 41.0 40-41 31.87613016712637 36.5 30.0 40.0 9.0 41.0 42-43 32.10519419443033 37.0 30.0 40.0 9.0 41.0 44-45 32.15703614568967 37.0 30.0 40.0 9.0 41.0 46-47 31.934117248554355 37.0 30.0 40.0 8.0 41.0 48-49 31.716684548220464 36.0 30.0 40.0 7.5 41.0 50-51 31.185751728424187 35.5 29.5 39.5 4.0 40.5 52-53 31.13675343567782 36.0 29.0 39.5 2.0 40.5 54-55 31.123679864992376 36.0 29.0 40.0 2.0 41.0 56-57 30.868491379049622 35.5 29.0 40.0 2.0 41.0 58-59 30.710586295698143 35.0 28.5 39.5 2.0 41.0 60-61 30.558535247453484 35.0 28.0 39.0 2.0 41.0 62-63 30.40005232150202 35.0 28.5 39.0 2.0 40.5 64-65 30.035140277576154 35.0 28.0 39.0 2.0 40.0 66-67 29.80255059761438 34.0 28.0 38.5 2.0 40.0 68-69 29.610979047204275 34.0 28.0 38.0 2.0 40.0 70-71 29.309227259804988 34.0 28.0 37.5 2.0 40.0 72-73 28.97511420755607 34.0 28.0 37.0 2.0 40.0 74-75 28.54966743334301 34.0 27.0 36.5 2.0 39.0 76-77 28.241121222569014 33.5 26.5 36.0 2.0 39.0 78-79 27.83673404194188 33.0 26.0 36.0 2.0 38.5 80-81 27.449462116813045 33.0 26.0 35.0 2.0 37.0 82-83 27.122225523819893 33.0 26.0 35.0 2.0 37.0 84-85 26.799353655549105 33.0 25.5 35.0 2.0 36.5 86-87 26.490660196039293 32.5 25.0 35.0 2.0 36.0 88-89 26.143882618373617 32.0 24.5 35.0 2.0 36.0 90-91 25.762380235174568 32.0 23.5 35.0 2.0 35.0 92-93 25.413227397413564 32.0 21.0 35.0 2.0 35.0 94-95 25.119717947787375 32.0 19.5 35.0 2.0 35.0 96-97 24.73070455590235 32.0 17.5 34.5 2.0 35.0 98-99 24.374456370520917 32.0 4.5 34.0 2.0 35.0 100-101 23.78364416237207 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.011796210000377 1101 2 5.766757342185503 1101 3 5.202879793152455 1101 4 6.510862275792299 1101 5 6.046170456614149 1101 6 5.515162640112898 1101 7 6.192119760961056 1101 8 5.5809019935516915 1101 9 6.308636383843378 1101 10-11 6.21635913182995 1101 12-13 5.966773121606426 1101 14-15 6.263734782894559 1101 16-17 6.259683687685662 1101 18-19 6.3790544212589175 1101 20-21 5.701033019016489 1101 22-23 5.736845243538717 1101 24-25 5.7286076852295515 1101 26-27 5.861636132732439 1101 28-29 5.823818759704 1101 30-31 5.834111138362136 1101 32-33 5.69428442922996 1101 34-35 5.6406505159494 1101 36-37 5.760959907439879 1101 38-39 5.640585378384529 1101 40-41 5.686666023685337 1101 42-43 5.603509060833467 1101 44-45 5.617618278606425 1101 46-47 5.244504555208422 1101 48-49 5.298051777468061 1101 50-51 5.279385929560696 1101 52-53 5.071013246207418 1101 54-55 5.346663499016525 1101 56-57 5.273001828317227 1101 58-59 5.146466395664969 1101 60-61 5.100335036944575 1101 62-63 5.012332934919229 1101 64-65 4.928870311548405 1101 66-67 4.914855347959069 1101 68-69 5.0582360169406755 1101 70-71 4.840230348790918 1101 72-73 4.598830235232352 1101 74-75 4.878706529797331 1101 76-77 4.730556808455052 1101 78-79 4.676426550437075 1101 80-81 4.619855508724502 1101 82-83 4.514228485776567 1101 84-85 4.422611151006947 1101 86-87 4.150483813839351 1101 88-89 4.284407439680795 1101 90-91 4.238516625631732 1101 92-93 4.233791668860189 1101 94-95 4.178735282779385 1101 96-97 4.072978977290319 1101 98-99 3.772708219409516 1101 100-101 3.8178988855657074 1102 1 5.477497207446621 1102 2 6.064203775886231 1102 3 5.7228202783853845 1102 4 7.017158896463741 1102 5 6.617121942861445 1102 6 6.123841010998163 1102 7 6.709951220624269 1102 8 6.150602507223958 1102 9 6.829099979044329 1102 10-11 6.875840668792794 1102 12-13 6.9011853472323885 1102 14-15 7.163720017220378 1102 16-17 7.094755290197238 1102 18-19 7.220885062498677 1102 20-21 6.398254870629092 1102 22-23 6.611911861534431 1102 24-25 6.449863680482228 1102 26-27 6.515107482554303 1102 28-29 6.567449815116522 1102 30-31 6.729303913338693 1102 32-33 6.2709556327454195 1102 34-35 6.362343170055848 1102 36-37 6.389177991457149 1102 38-39 6.244442228255643 1102 40-41 6.42990504017401 1102 42-43 6.502552286243812 1102 44-45 6.35395566340004 1102 46-47 6.322827283235711 1102 48-49 6.026428174170938 1102 50-51 5.99190654609658 1102 52-53 5.817344761425378 1102 54-55 6.171532402286534 1102 56-57 6.0371664177353495 1102 58-59 6.115168964055652 1102 60-61 5.979196844177963 1102 62-63 5.801352513234473 1102 64-65 5.88500803976871 1102 66-67 5.859822682207405 1102 68-69 5.907581887300111 1102 70-71 5.716601512632806 1102 72-73 5.582041252714998 1102 74-75 5.723070012553123 1102 76-77 5.63306057362858 1102 78-79 5.529370709915749 1102 80-81 5.611290683818833 1102 82-83 5.633158579115882 1102 84-85 5.559830718783665 1102 86-87 5.5492864732458855 1102 88-89 5.6063171902950195 1102 90-91 5.522118331698234 1102 92-93 5.430500171114449 1102 94-95 5.343430306292181 1102 96-97 5.213243268180559 1102 98-99 4.9976241497372556 1102 100-101 4.985447896669168 1103 1 4.881910748529467 1103 2 5.530389229458557 1103 3 5.131145193062743 1103 4 6.496558047179626 1103 5 6.041648886250407 1103 6 5.440872495800498 1103 7 5.939414603646121 1103 8 5.370805611426135 1103 9 6.114395995301365 1103 10-11 6.106728013581705 1103 12-13 6.2057793844935425 1103 14-15 6.346040407574137 1103 16-17 6.239549740021079 1103 18-19 6.153317386020536 1103 20-21 5.438184886060387 1103 22-23 5.650984013979887 1103 24-25 5.424002142223852 1103 26-27 5.656526483431708 1103 28-29 5.392702525302301 1103 30-31 5.593690022420393 1103 32-33 5.385493510677044 1103 34-35 5.371637579874086 1103 36-37 5.371053338835946 1103 38-39 5.19087025175719 1103 40-41 5.401664697547336 1103 42-43 5.419237984704836 1103 44-45 5.106056103500574 1103 46-47 5.255577952358649 1103 48-49 5.02843869875322 1103 50-51 5.02614836116269 1103 52-53 4.817384669932938 1103 54-55 5.185714269142434 1103 56-57 5.140306678369946 1103 58-59 5.156725299720577 1103 60-61 5.002780708750823 1103 62-63 4.917719822398983 1103 64-65 4.8156563267712755 1103 66-67 5.046003006905352 1103 68-69 5.130368072081744 1103 70-71 4.949842366981709 1103 72-73 4.81037935662005 1103 74-75 4.791093214058268 1103 76-77 4.840313706980478 1103 78-79 4.8695965872135005 1103 80-81 4.877249862950354 1103 82-83 4.773710638064731 1103 84-85 4.726620873636442 1103 86-87 4.637175275326488 1103 88-89 4.610815903512677 1103 90-91 4.61198328461008 1103 92-93 4.532034405622664 1103 94-95 4.599726917195689 1103 96-97 4.282668583729652 1103 98-99 4.121049875635141 1103 100-101 4.125895207923289 1104 1 4.638366242323727 1104 2 5.504515857037937 1104 3 5.1621046082763264 1104 4 6.534732493231559 1104 5 6.163587514134363 1104 6 5.805293420189436 1104 7 6.388592247748768 1104 8 5.740012190273312 1104 9 6.411465971696707 1104 10-11 6.412171172037507 1104 12-13 6.420597971259699 1104 14-15 6.5131393187140105 1104 16-17 6.411294771620863 1104 18-19 6.569398060656955 1104 20-21 5.92674576324001 1104 22-23 6.101784097738353 1104 24-25 5.8385433742348205 1104 26-27 6.005561357413281 1104 28-29 6.0492429197285595 1104 30-31 6.166109119351784 1104 32-33 6.013884967313004 1104 34-35 5.777172111207367 1104 36-37 5.866480126317864 1104 38-39 5.770001447980448 1104 40-41 6.0650923226139675 1104 42-43 6.024712362227113 1104 44-45 6.051710371375396 1104 46-47 6.044803186501646 1104 48-49 5.791075377349117 1104 50-51 5.65769103991088 1104 52-53 5.3495595347772245 1104 54-55 5.6902878623808455 1104 56-57 5.609311475446013 1104 58-59 5.512123533178272 1104 60-61 5.333194742101924 1104 62-63 5.338721687928562 1104 64-65 5.47826273810151 1104 66-67 5.452414527502324 1104 68-69 5.392018304321141 1104 70-71 5.385512434749927 1104 72-73 5.297226992346843 1104 74-75 5.30185198966171 1104 76-77 5.2549807670581465 1104 78-79 5.1164628782361135 1104 80-81 5.142921263239696 1104 82-83 4.9629183590728765 1104 84-85 4.920455869448517 1104 86-87 4.825144322764242 1104 88-89 4.856228985062973 1104 90-91 4.822788127372586 1104 92-93 4.717580358565634 1104 94-95 4.669701218573898 1104 96-97 4.644086195851788 1104 98-99 4.481084330824359 1104 100-101 4.656440018705293 1105 1 4.566744521538549 1105 2 5.245822956652688 1105 3 4.94970458993107 1105 4 6.3392243295918576 1105 5 3.5326156741050063 1105 6 4.927646199495989 1105 7 5.755800099519085 1105 8 1.116192248978141 1105 9 0.7251143848319082 1105 10-11 2.3090132652314175 1105 12-13 5.534065683606062 1105 14-15 3.6461766054503926 1105 16-17 5.82445657679423 1105 18-19 6.435708141376086 1105 20-21 3.3273025418127453 1105 22-23 5.465956497298066 1105 24-25 5.563750446331685 1105 26-27 5.739967888325037 1105 28-29 3.4294032114181583 1105 30-31 5.605728455253452 1105 32-33 5.747092406315019 1105 34-35 5.718254940046112 1105 36-37 5.678580199698896 1105 38-39 5.590505025305703 1105 40-41 5.7409047882166 1105 42-43 3.1608211037424283 1105 44-45 5.260167764514268 1105 46-47 5.569310845646754 1105 48-49 5.54386683763164 1105 50-51 5.558430583113967 1105 52-53 5.415161259231457 1105 54-55 5.65316302290184 1105 56-57 5.515706173666523 1105 58-59 5.408459755889705 1105 60-61 5.270960977655033 1105 62-63 5.1542353551455875 1105 64-65 5.282759390091972 1105 66-67 5.336923333226043 1105 68-69 5.291441804596321 1105 70-71 5.1697141867220395 1105 72-73 5.089821929055706 1105 74-75 5.3346345066310725 1105 76-77 5.068363403271682 1105 78-79 5.00430524660889 1105 80-81 5.004815869733921 1105 82-83 4.8510125161082875 1105 84-85 4.904220087023276 1105 86-87 4.912406751676368 1105 88-89 4.939315574679554 1105 90-91 4.8954009507077565 1105 92-93 4.951628010372641 1105 94-95 4.81392567280248 1105 96-97 4.671642836835094 1105 98-99 4.703995952545142 1105 100-101 4.837518719861041 1106 1 4.342621794403801 1106 2 4.772737627447697 1106 3 4.8040731204231335 1106 4 6.320660446521103 1106 5 6.287113215312544 1106 6 5.408994285584079 1106 7 6.112507897837389 1106 8 5.598417987374766 1106 9 6.201478483426449 1106 10-11 6.245222209468434 1106 12-13 6.443992299275546 1106 14-15 6.152659963068089 1106 16-17 6.160264639205057 1106 18-19 6.3703325304724 1106 20-21 5.6533733307512115 1106 22-23 5.780077289272079 1106 24-25 5.770988896078219 1106 26-27 5.960550409649752 1106 28-29 5.8757649195200266 1106 30-31 6.000428012063704 1106 32-33 5.81705017427786 1106 34-35 5.395299000999881 1106 36-37 5.596837492598748 1106 38-39 5.663551651296046 1106 40-41 5.798417575455165 1106 42-43 2.6784069717568784 1106 44-45 4.983717722016625 1106 46-47 5.258189173364329 1106 48-49 5.185181570655345 1106 50-51 5.257830493565351 1106 52-53 5.089861119596325 1106 54-55 5.321870335532754 1106 56-57 5.264992061412023 1106 58-59 5.310374236963074 1106 60-61 5.158423602491872 1106 62-63 5.209242488679266 1106 64-65 5.220214046619912 1106 66-67 5.330196562743694 1106 68-69 5.373201966087379 1106 70-71 5.345684992356013 1106 72-73 5.0123528916274225 1106 74-75 5.16214262671728 1106 76-77 5.04505401280937 1106 78-79 5.0461493030093365 1106 80-81 5.009487575407359 1106 82-83 5.122185640899591 1106 84-85 5.085560394524112 1106 86-87 5.131908997980588 1106 88-89 5.294402011858924 1106 90-91 5.2102333885549434 1106 92-93 5.201432407361377 1106 94-95 5.15665174510919 1106 96-97 5.170975206382494 1106 98-99 5.035842437382591 1106 100-101 5.211570870545561 1107 1 2.830972318803557 1107 2 -0.19832070660795154 1107 3 0.6487617506799346 1107 4 2.3096526481595703 1107 5 2.1920864109072227 1107 6 3.671122983501977 1107 7 4.696931136902215 1107 8 4.1664355120187935 1107 9 4.644738508484167 1107 10-11 2.582922914183964 1107 12-13 4.783417232494362 1107 14-15 3.1386428744388866 1107 16-17 1.6330424738923988 1107 18-19 2.3088640272902836 1107 20-21 1.9245384359069568 1107 22-23 4.0909378669654615 1107 24-25 4.055217898418515 1107 26-27 3.019831878231731 1107 28-29 3.852177305492962 1107 30-31 3.191343932120649 1107 32-33 3.748768414143804 1107 34-35 4.429628339281717 1107 36-37 4.6048780262178575 1107 38-39 4.556783834443294 1107 40-41 3.221820539830656 1107 42-43 3.91799291526932 1107 44-45 4.223307868081587 1107 46-47 4.469540793054335 1107 48-49 1.9360585908354757 1107 50-51 3.6164051453444728 1107 52-53 3.860072376658586 1107 54-55 4.396985840062317 1107 56-57 2.993230193797679 1107 58-59 2.1942561347307326 1107 60-61 3.2110578149466633 1107 62-63 4.084493788187306 1107 64-65 4.3765957539594105 1107 66-67 4.2785332712520265 1107 68-69 4.201441411770702 1107 70-71 4.142732699206963 1107 72-73 3.9608460138875543 1107 74-75 4.108101215815335 1107 76-77 4.0186139162557275 1107 78-79 3.861588955668374 1107 80-81 4.001014704197207 1107 82-83 3.8680055457643086 1107 84-85 3.866357618738167 1107 86-87 3.9071610854672265 1107 88-89 3.935998220105912 1107 90-91 4.040339161548818 1107 92-93 4.140521530367465 1107 94-95 3.9592070283743652 1107 96-97 4.01972547232527 1107 98-99 4.002640039826375 1107 100-101 4.042804766232951 1108 1 -2.3218630134292972 1108 2 -1.4975528826115685 1108 3 -2.1726671246747067 1108 4 -4.404456151502355 1108 5 -9.062703486186969 1108 6 -7.497240999070517 1108 7 -6.098284559725837 1108 8 -6.4970150238058295 1108 9 -8.098011498209615 1108 10-11 -3.6021304939288257 1108 12-13 -2.2072284864608456 1108 14-15 -6.905307489007768 1108 16-17 -6.112776711682397 1108 18-19 -4.642879480930414 1108 20-21 -5.117750986395411 1108 22-23 -4.147831049207788 1108 24-25 -8.7353759722318 1108 26-27 -9.793484543172529 1108 28-29 -6.625916941358522 1108 30-31 -6.666580711826992 1108 32-33 -3.13583195435692 1108 34-35 -1.387154581330389 1108 36-37 -5.076958524595451 1108 38-39 -5.826679472503031 1108 40-41 -3.8154102480165832 1108 42-43 -4.793802480692534 1108 44-45 -2.2578324147926345 1108 46-47 -2.9528846054750204 1108 48-49 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-8.32980779748005 2313 48-49 -6.912111121506715 2313 50-51 -4.893538937205822 2313 52-53 -5.122116666595769 2313 54-55 -8.845976439678658 2313 56-57 -7.018543215629499 2313 58-59 -7.043923377576871 2313 60-61 -5.74016910229167 2313 62-63 -8.131518586670982 2313 64-65 -7.60506678134929 2313 66-67 -9.259479065175022 2313 68-69 -9.304943938241784 2313 70-71 -10.543309075547556 2313 72-73 -9.136851955021577 2313 74-75 -9.577135424525935 2313 76-77 -8.799487196328585 2313 78-79 -9.975281973022467 2313 80-81 -11.487256082298057 2313 82-83 -10.944455782803551 2313 84-85 -9.538874794167041 2313 86-87 -8.663162313391364 2313 88-89 -9.443948360474787 2313 90-91 -9.536794252490111 2313 92-93 -8.87961643777304 2313 94-95 -9.22847383863434 2313 96-97 -9.048253621081852 2313 98-99 -8.42613534880951 2313 100-101 -8.263880347427433 2314 1 -13.44920292763675 2314 2 -14.846294419641906 2314 3 -14.221847960893632 2314 4 -24.984615332855398 2314 5 -19.20240062815813 2314 6 -17.65089235056155 2314 7 -18.031071783871788 2314 8 -14.004612315655539 2314 9 -15.620431261504878 2314 10-11 -14.446613412942728 2314 12-13 -15.070014676961645 2314 14-15 -16.363036570614053 2314 16-17 -17.27522696750957 2314 18-19 -16.193892453177043 2314 20-21 -16.56868901936869 2314 22-23 -17.88485621846718 2314 24-25 -18.070923028927236 2314 26-27 -17.432778563535337 2314 28-29 -19.113138503196343 2314 30-31 -19.654019339146238 2314 32-33 -16.641757325177117 2314 34-35 -18.085614450057456 2314 36-37 -17.27157323336593 2314 38-39 -16.951065745695445 2314 40-41 -18.583455108216075 2314 42-43 -18.161433882832178 2314 44-45 -15.911540616766898 2314 46-47 -15.279090094130765 2314 48-49 -15.096800116722026 2314 50-51 -14.773682477875681 2314 52-53 -14.798671690519217 2314 54-55 -15.169899884654733 2314 56-57 -14.534571923763473 2314 58-59 -13.781483186189313 2314 60-61 -13.962417906119422 2314 62-63 -15.801614280450885 2314 64-65 -14.360808408143548 2314 66-67 -14.781488634553014 2314 68-69 -17.300876952595853 2314 70-71 -16.95383539133703 2314 72-73 -15.9507275531077 2314 74-75 -14.792446429310623 2314 76-77 -14.582262315945808 2314 78-79 -14.480305896467442 2314 80-81 -14.092997709092316 2314 82-83 -15.079384012468623 2314 84-85 -15.196530296559386 2314 86-87 -15.426080973678447 2314 88-89 -14.536053623632682 2314 90-91 -14.220047841006858 2314 92-93 -14.058803040643854 2314 94-95 -13.86220589605061 2314 96-97 -13.607583764622522 2314 98-99 -13.893599463642044 2314 100-101 -13.549765514891547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94149.0 3 15606.0 4 10655.0 5 8699.0 6 8875.0 7 10036.0 8 12099.0 9 13904.0 10 15102.0 11 16309.0 12 17823.0 13 19153.0 14 20658.0 15 23562.0 16 25329.0 17 26588.0 18 24671.0 19 22373.0 20 22568.0 21 24187.0 22 28089.0 23 34158.0 24 42224.0 25 51253.0 26 61399.0 27 70345.0 28 77446.0 29 85522.0 30 96023.0 31 106259.0 32 121347.0 33 145300.0 34 174339.0 35 210109.0 36 258059.0 37 310231.0 38 273945.0 39 66724.0 40 66.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36381698309922 19.888379785641977 14.18207721186278 23.565726019396028 2 14.874362293459226 20.269207340287824 40.938056803472165 23.918373562780783 3 18.16463429387143 25.36935048752752 30.383103784084586 26.082911434516465 4 11.697404007922142 17.355658216179545 39.535068558164156 31.411869217734157 5 12.695594841354799 37.3758509168967 35.62197217575164 14.306582065996858 6 30.05327844039474 37.75666737300963 18.43286462432645 13.757189562269176 7 26.390867324154737 32.20199214344644 22.806257994686607 18.600882537712213 8 24.750119495315367 35.67821680821295 20.952874377971536 18.618789318500145 9 25.095831912585098 16.39547878490168 21.49234763870697 37.016341663806244 10-11 23.73747282093079 26.551162171507737 29.339381500773193 20.371983506788283 12-13 24.730362902286004 24.255543148283493 28.91934260449 22.0947513449405 14-15 22.007820084674364 25.86126646606334 26.758908610402717 25.37200483885958 16-17 21.377518274590233 28.81403346198085 27.461105278409153 22.347342985019765 18-19 21.231925197710755 27.905574532438347 29.428972782744083 21.433527487106808 20-21 22.083940634777775 27.23033071892506 29.42219848736996 21.263530158927207 22-23 21.880276475738548 27.056901873895743 29.137206393070965 21.925615257294748 24-25 21.735734486821464 27.259898684426375 29.147933868816523 21.85643295993564 26-27 21.581518185809937 27.362831764572004 29.24917014479082 21.80647990482724 28-29 21.524758376974496 27.28060440054309 29.313066802912253 21.881570419570163 30-31 21.762235856261515 27.18783270825585 29.205342448770615 21.844588986712015 32-33 21.633860852788427 27.40412289435949 29.07296753251914 21.889048720332944 34-35 21.942038818740496 27.40110641323637 29.095711067956458 21.561143700066676 36-37 21.79044711777058 27.152430693963215 28.973941694840317 22.083180493425896 38-39 21.69720668382068 27.45355592546993 29.062506801829468 21.78673058887992 40-41 21.84395042759464 27.167146502371427 29.131158351459234 21.857744718574704 42-43 21.73402794059015 27.323148863523905 28.944542494549847 21.9982807013361 44-45 21.853149763916228 27.52716344534731 28.723456184683428 21.896230606053038 46-47 21.906766665790055 27.450391286522237 28.57128532273026 22.071556724957446 48-49 21.847556879996215 27.6454093940684 28.548431956861076 21.95860176907431 50-51 21.68991883779629 27.71177901905388 28.391012565744944 22.20728957740489 52-53 21.745282617271197 27.853667719546195 28.27572767408223 22.12532198910038 54-55 21.77729662910484 27.73306613150463 28.231225643033397 22.25841159635713 56-57 21.78951574197456 27.798163972003902 28.100963904268344 22.311356381753196 58-59 21.750140104896133 27.852094745995277 28.246702200998136 22.151062948110457 60-61 21.93161319344417 27.835875602245856 28.123367063645325 22.10914414066464 62-63 21.777936808932765 27.796629648447897 28.197660351794053 22.22777319082529 64-65 21.704841704773656 27.760412886211316 28.22421805061576 22.310527358399266 66-67 21.781263609639254 27.69442503810699 28.14059437831168 22.38371697394208 68-69 21.779430088795333 27.712268787350897 28.124395127144275 22.38390599670949 70-71 21.856664791560814 27.582844898502334 28.08468144371053 22.47580886622632 72-73 21.96189376618035 27.647207150806903 28.05956031792117 22.331338765091576 74-75 21.754025428854852 27.868269277297912 28.04453300791174 22.333172285935497 76-77 21.851353251796475 27.780071254022403 27.923728557257267 22.44484693692386 78-79 21.956260887711405 27.773398750332678 27.827137923108562 22.443202438847354 80-81 21.91936364351213 27.85265599670949 27.84906456412862 22.378915795649753 82-83 22.07642266095667 27.77908833563185 27.933160793351234 22.21132821006025 84-85 22.07723545885655 27.81280999733856 27.742191091432584 22.367763452372312 86-87 21.923824580823112 27.797763785052382 27.91348352326341 22.364928110861097 88-89 22.08343540562774 27.679511141758002 27.80893503060657 22.428118422007696 90-91 22.122714336696426 27.719489457066125 27.825965981950596 22.331830224286854 92-93 22.188966816675133 27.688754355084562 27.79664855072464 22.325630277515664 94-95 21.9776582649827 27.627737805763232 27.865358326679733 22.52924560257434 96-97 22.01122870847548 27.6014447388159 27.888362397474054 22.498964155234567 98-99 22.019375589751032 27.64435290701894 27.670532560305823 22.665738942924197 100-101 22.105456558031502 27.54362267426387 27.672139253828842 22.678781513875784 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 330.0 1 229.0 2 163.5 3 206.5 4 187.0 5 187.0 6 284.0 7 540.0 8 984.0 9 1327.0 10 1336.0 11 1227.5 12 1337.0 13 1561.5 14 1735.0 15 1929.5 16 2155.0 17 2372.0 18 2646.0 19 3031.0 20 3522.0 21 4149.5 22 5161.5 23 6573.5 24 8402.0 25 10976.0 26 14537.5 27 19088.0 28 24061.0 29 29915.5 30 36514.5 31 43303.5 32 50466.5 33 57594.5 34 64540.0 35 71108.0 36 78796.5 37 85035.5 38 87739.0 39 89498.0 40 90723.0 41 91645.0 42 92535.0 43 93967.0 44 95973.0 45 98305.0 46 98124.5 47 96640.0 48 96163.0 49 95101.0 50 93167.5 51 89959.0 52 86409.0 53 82610.5 54 77804.0 55 71521.0 56 65297.5 57 58525.0 58 51157.0 59 44351.0 60 36916.5 61 29711.5 62 23481.0 63 18118.5 64 13548.0 65 9885.5 66 7157.5 67 5295.0 68 3998.0 69 3055.0 70 2346.5 71 1741.0 72 1314.5 73 958.5 74 724.0 75 574.0 76 424.0 77 321.0 78 236.5 79 166.5 80 128.0 81 106.5 82 88.5 83 66.0 84 45.5 85 42.5 86 44.0 87 31.5 88 21.5 89 18.5 90 15.5 91 13.0 92 7.0 93 2.5 94 4.5 95 4.5 96 2.5 97 2.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06638479591589848 2 0.7025598219254313 3 0.0 4 0.5894485979047204 5 0.23132606276160753 6 0.09315041978176188 7 0.10600396796593356 8 0.10664664537514215 9 0.10573933609155356 10-11 0.18240697055478938 12-13 0.24567289080835208 14-15 0.1380811315961385 16-17 0.13116289830877548 18-19 0.1828606251965837 20-21 0.11458560160654231 22-23 0.10791309791681788 24-25 0.10012535989934916 26-27 0.1705930475913963 28-29 0.10950088916309791 30-31 0.13275068955505553 32-33 0.1064954271612107 34-35 0.15261698241029736 36-37 0.13348787834797127 38-39 0.1297263252764269 40-41 0.10534238827998356 42-43 0.10689237497278073 44-45 0.09367968353052188 46-47 0.09177055357963756 48-49 0.09674185236263337 50-51 0.08335904042970169 52-53 0.09192177179356899 54-55 0.30154802085601606 56-57 0.31980762018823644 58-59 0.33141361810747383 60-61 0.16014008855338607 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2645184.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.4866452721601 #Duplication Level Percentage of deduplicated Percentage of total 1 83.7580175749742 58.200636559315846 2 10.015778582228013 13.919257069355531 3 2.8188902138048864 5.8762567304347115 4 1.1544760492078825 3.208826708260519 5 0.6358340110147452 2.209098618767817 6 0.37298805577025285 1.555061323323614 7 0.24616883581462232 1.1973812599917901 8 0.17371603681346043 0.9656755702513946 9 0.12834291393390357 0.802630668034848 >10 0.6415102922750412 8.010213507525629 >50 0.03943155692895749 1.8292501819575586 >100 0.014287921572591449 1.588674257423095 >500 3.905689630726945E-4 0.16613145165091803 >1k 1.1159113229219248E-4 0.2521631181844559 >5k 5.579556614609624E-5 0.2187429755222479 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5642 0.2132932907502843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4141 0.15654865597251458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.7804553482857906E-5 0.0 0.0 0.0 5 0.0 3.7804553482857906E-5 0.0 0.0 0.0 6 0.0 3.7804553482857906E-5 0.0 0.0 0.0 7 0.0 3.7804553482857906E-5 0.0 0.0 0.0 8 3.7804553482857906E-5 7.560910696571581E-5 0.0 0.0 0.0 9 3.7804553482857906E-5 1.1341366044857371E-4 0.0 0.0 0.0 10-11 1.1341366044857371E-4 1.1341366044857371E-4 0.0 1.8902276741428953E-5 0.0 12-13 1.1341366044857371E-4 1.1341366044857371E-4 0.0 9.451138370714476E-5 0.0 14-15 1.1341366044857371E-4 1.5121821393143163E-4 1.8902276741428953E-5 1.1341366044857371E-4 1.8902276741428953E-5 16-17 1.3231593719000267E-4 1.5121821393143163E-4 3.7804553482857906E-5 1.1341366044857371E-4 3.7804553482857906E-5 18-19 1.5121821393143163E-4 1.5121821393143163E-4 3.7804553482857906E-5 1.1341366044857371E-4 5.6706830224286856E-5 20-21 1.5121821393143163E-4 1.7012049067286058E-4 3.7804553482857906E-5 1.1341366044857371E-4 7.560910696571581E-5 22-23 1.5121821393143163E-4 1.890227674142895E-4 3.7804553482857906E-5 2.457295976385764E-4 7.560910696571581E-5 24-25 1.5121821393143163E-4 2.0792504415571847E-4 3.7804553482857906E-5 4.5365464179429485E-4 1.1341366044857371E-4 26-27 1.5121821393143163E-4 2.2682732089714743E-4 3.7804553482857906E-5 8.695047301057318E-4 1.1341366044857371E-4 28-29 1.7012049067286058E-4 2.2682732089714743E-4 7.560910696571581E-5 0.0022493709322300454 1.1341366044857371E-4 30-31 1.890227674142895E-4 2.6463187438000534E-4 7.560910696571581E-5 0.007844444847693016 1.1341366044857371E-4 32-33 1.890227674142895E-4 2.6463187438000534E-4 7.560910696571581E-5 0.02181322735960901 1.1341366044857371E-4 34-35 1.890227674142895E-4 3.213387046042922E-4 7.560910696571581E-5 0.04366425927270088 1.1341366044857371E-4 36-37 1.890227674142895E-4 4.1585008831143694E-4 7.560910696571581E-5 0.07921944182332874 1.1341366044857371E-4 38-39 1.890227674142895E-4 4.914591952771527E-4 7.560910696571581E-5 0.12760927028138686 1.1341366044857371E-4 40-41 1.890227674142895E-4 4.914591952771527E-4 7.560910696571581E-5 0.17734116038808642 1.1341366044857371E-4 42-43 1.890227674142895E-4 4.914591952771527E-4 7.560910696571581E-5 0.22951144419443031 1.1341366044857371E-4 44-45 1.890227674142895E-4 4.914591952771527E-4 7.560910696571581E-5 0.2959529469405531 1.1341366044857371E-4 46-47 1.890227674142895E-4 4.914591952771527E-4 7.560910696571581E-5 0.37364130434782605 1.1341366044857371E-4 48-49 1.890227674142895E-4 4.914591952771527E-4 7.560910696571581E-5 0.45724607437516634 1.1341366044857371E-4 50-51 1.890227674142895E-4 6.804819626914422E-4 7.560910696571581E-5 0.5415124240884566 1.1341366044857371E-4 52-53 1.890227674142895E-4 6.804819626914422E-4 7.560910696571581E-5 0.623359282378844 1.3231593719000267E-4 54-55 1.890227674142895E-4 7.182865161743002E-4 7.560910696571581E-5 0.7164907998838643 1.5121821393143163E-4 56-57 1.890227674142895E-4 7.182865161743002E-4 7.560910696571581E-5 0.8266343664561709 1.5121821393143163E-4 58-59 1.890227674142895E-4 7.182865161743002E-4 7.560910696571581E-5 0.9508412269241006 1.5121821393143163E-4 60-61 1.890227674142895E-4 7.182865161743002E-4 7.560910696571581E-5 1.077770015242796 1.890227674142895E-4 62-63 1.890227674142895E-4 7.182865161743002E-4 7.560910696571581E-5 1.2076097541796715 2.2682732089714743E-4 64-65 1.890227674142895E-4 7.93895623140016E-4 7.560910696571581E-5 1.3457097880525515 2.2682732089714743E-4 66-67 1.890227674142895E-4 8.127978998814449E-4 7.560910696571581E-5 1.5043565967433645 2.2682732089714743E-4 68-69 1.890227674142895E-4 8.695047301057317E-4 7.560910696571581E-5 1.680998372892018 2.2682732089714743E-4 70-71 1.890227674142895E-4 8.884070068471608E-4 7.560910696571581E-5 1.8693217560668747 2.2682732089714743E-4 72-73 1.890227674142895E-4 9.262115603300186E-4 7.560910696571581E-5 2.0594408555321673 2.2682732089714743E-4 74-75 1.890227674142895E-4 0.0010018206672957343 7.560910696571581E-5 2.2519416418668796 2.457295976385764E-4 76-77 1.890227674142895E-4 0.0010207229440371635 7.560910696571581E-5 2.465008105296267 2.6463187438000534E-4 78-79 1.890227674142895E-4 0.0010396252207785924 7.560910696571581E-5 2.7001902325131257 2.6463187438000534E-4 80-81 1.890227674142895E-4 0.0010585274975200214 7.560910696571581E-5 2.961873351721468 3.024364278628632E-4 82-83 1.890227674142895E-4 0.0010585274975200214 7.560910696571581E-5 3.231854570419298 3.402409813457211E-4 84-85 1.890227674142895E-4 0.0010585274975200214 7.560910696571581E-5 3.498603499794343 3.402409813457211E-4 86-87 1.890227674142895E-4 0.0010963320510028792 7.560910696571581E-5 3.784972992426992 3.402409813457211E-4 88-89 1.890227674142895E-4 0.001134136604485737 7.560910696571581E-5 4.096104467590912 3.402409813457211E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2810 0.0 53.34959 1 GTATCAA 5505 0.0 42.07 1 ATCAACG 6600 0.0 34.074562 3 CAACGCA 6710 0.0 33.419228 5 TCAACGC 6880 0.0 32.907913 4 TATCAAC 7140 0.0 32.236294 2 AACGCAG 7110 0.0 31.848513 6 ACGCAGA 7945 0.0 27.736067 7 CGCAGAG 8010 0.0 27.272282 8 GTACATG 5610 0.0 24.409832 1 GCAGAGT 8815 0.0 23.216005 9 TACATGG 6025 0.0 21.214478 2 GAGTACT 5620 0.0 21.129236 12-13 ACATGGG 5900 0.0 20.717686 3 AGAGTAC 8015 0.0 20.485209 10-11 CAGAGTA 8845 0.0 19.343094 10-11 GTACTTT 6475 0.0 18.468853 14-15 TAGTACT 1235 0.0 17.404278 4 AGTACTT 6485 0.0 17.210062 12-13 TGGTATC 1540 0.0 16.84926 2 >>END_MODULE