FastQCFastQC Report
Sat 18 Jun 2016
SRR1931141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645184
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71560.27052938472333116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60730.22958705330139603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37670.14240975296992572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.053.259771
GTATCAA69000.039.823521
ATCAACG83500.031.8476813
CAACGCA85750.030.9554795
TCAACGC86300.030.8132174
TATCAAC88950.030.0031342
AACGCAG90300.029.6060496
ACGCAGA103350.025.5920187
CGCAGAG103600.025.2094158
GTACATG70000.021.6647431
GCAGAGT116500.021.4397479
GAGTACT72200.021.37499412-13
ACATGGG67600.021.1444323
ACGTATA2509.536961E-920.893567
GTGGTAT18550.020.5025061
TACATGG72600.020.3422972
AGAGTAC104200.020.02861810-11
CAGAGTA114550.019.33821510-11
GTACTTT79550.019.28067214-15
TGGTATC20000.019.23232
Produced by FastQC (version 0.11.5)