##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931141_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2645184 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25362734690668 31.0 31.0 34.0 30.0 34.0 2 31.270226570249932 31.0 31.0 34.0 28.0 34.0 3 31.423493034889066 31.0 31.0 34.0 28.0 34.0 4 35.024637227504776 35.0 35.0 37.0 32.0 37.0 5 34.907787133144616 35.0 35.0 37.0 32.0 37.0 6 35.04051135951223 36.0 35.0 37.0 32.0 37.0 7 34.896670326147444 36.0 35.0 37.0 32.0 37.0 8 34.94982655270862 36.0 35.0 37.0 32.0 37.0 9 36.32756473651738 38.0 35.0 39.0 32.0 39.0 10-11 36.20628375946626 38.0 35.0 39.0 31.5 39.0 12-13 36.13563857939561 38.0 35.0 39.0 31.0 39.0 14-15 37.08126258891631 39.0 36.0 40.0 31.0 41.0 16-17 37.12084244423072 39.0 36.0 40.0 31.0 41.0 18-19 37.19779228968571 39.0 36.0 40.0 31.0 41.0 20-21 37.18060274823982 39.0 36.0 40.0 31.0 41.0 22-23 37.067897545123515 39.0 36.0 40.0 31.0 41.0 24-25 36.92774396790544 39.0 36.0 40.0 31.0 41.0 26-27 36.6805303525199 39.0 35.5 40.0 30.0 41.0 28-29 36.40463612361182 38.0 35.0 40.0 30.0 41.0 30-31 36.18689456007597 38.0 35.0 40.0 29.5 41.0 32-33 36.044377820219694 38.0 34.5 40.0 29.5 41.0 34-35 35.81635020474946 38.0 34.0 40.0 29.0 41.0 36-37 36.0524129890397 38.0 35.0 40.0 30.0 41.0 38-39 36.0450535766132 38.0 35.0 40.0 30.0 41.0 40-41 35.89417314636714 38.0 35.0 40.0 29.0 41.0 42-43 35.73572726887808 38.0 34.5 40.0 28.5 41.0 44-45 35.49960456437057 38.0 34.0 40.0 27.0 41.0 46-47 35.30631687625511 38.0 34.0 40.0 27.0 41.0 48-49 35.04222314969394 38.0 34.0 40.0 26.0 41.0 50-51 34.70043255970095 38.0 33.0 40.0 25.0 41.0 52-53 34.58500902016647 38.0 33.0 40.0 24.5 41.0 54-55 34.35671412650311 38.0 33.0 40.0 24.0 41.0 56-57 34.134203140499864 37.0 33.0 40.0 24.0 41.0 58-59 33.88886803337688 37.0 32.5 40.0 23.5 41.0 60-61 33.595540045607414 37.0 32.0 40.0 23.0 41.0 62-63 33.33744930409378 36.5 32.0 39.5 22.5 41.0 64-65 33.078377723440035 36.0 31.0 39.0 22.0 41.0 66-67 32.83117639453437 36.0 31.0 39.0 22.0 40.0 68-69 32.50697342793545 35.0 31.0 39.0 21.5 40.0 70-71 31.695845922249646 35.0 30.0 38.0 18.0 40.0 72-73 31.643505706975393 35.0 30.0 37.5 19.5 40.0 74-75 31.329107729367784 34.5 30.0 37.0 18.5 39.0 76-77 30.609371597590187 34.0 29.5 36.0 18.0 39.0 78-79 30.63527660079601 34.0 30.0 36.0 17.5 39.0 80-81 30.356592017795357 34.0 30.0 36.0 14.5 37.5 82-83 30.00470061818006 34.0 29.0 35.0 12.0 37.0 84-85 29.64971983045414 34.0 29.0 35.0 7.5 37.0 86-87 29.32046523039607 34.0 29.0 35.0 7.0 36.0 88-89 29.010590189567154 34.0 29.0 35.0 2.0 36.0 90-91 28.6974269086763 34.0 29.0 35.0 2.0 36.0 92-93 28.366954623950548 33.5 29.0 35.0 2.0 35.0 94-95 27.985249041276525 33.0 27.5 35.0 2.0 35.0 96-97 27.61188238700975 33.0 27.0 35.0 2.0 35.0 98-99 27.136116996019936 33.0 26.5 35.0 2.0 35.0 100-101 26.459988983753114 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.875348578375668 1101 2 0.8030134526168169 1101 3 0.6077650924377984 1101 4 0.8888640837296222 1101 5 0.7622271427488485 1101 6 0.8825127005246216 1101 7 0.7730636252559222 1101 8 0.8387760459760969 1101 9 1.2051687424422184 1101 10-11 0.9636536556194244 1101 12-13 1.029381781699783 1101 14-15 1.4218269313644782 1101 16-17 1.224237217532611 1101 18-19 1.0058619912340774 1101 20-21 0.9515861126361855 1101 22-23 0.9093891382397885 1101 24-25 0.7169872845590959 1101 26-27 1.0165347329637484 1101 28-29 0.8301598102846341 1101 30-31 1.1016946548821167 1101 32-33 1.1962936714864014 1101 34-35 0.9556280112338911 1101 36-37 1.166535974824079 1101 38-39 1.0759907520938938 1101 40-41 1.1240338826163239 1101 42-43 1.1042222028394022 1101 44-45 1.1910782886113083 1101 46-47 1.2529923557684342 1101 48-49 0.9312291101415795 1101 50-51 0.8294920871636293 1101 52-53 0.7720243723451929 1101 54-55 0.8231136756039064 1101 56-57 0.6009020428576193 1101 58-59 0.8194492952927348 1101 60-61 0.9884295435665109 1101 62-63 0.9238843944891926 1101 64-65 0.884701206177489 1101 66-67 0.653529637769914 1101 68-69 0.4953438810645494 1101 70-71 0.908292461915444 1101 72-73 0.38708682079877477 1101 74-75 -0.008608882349346203 1101 76-77 0.08056510750792611 1101 78-79 0.19859856791139663 1101 80-81 0.09346958091845536 1101 82-83 0.023615796332123296 1101 84-85 0.15183834762261483 1101 86-87 0.01676020583361293 1101 88-89 -0.09435564567089116 1101 90-91 -0.04186897771439746 1101 92-93 -0.20264888622603294 1101 94-95 -0.15345603643088168 1101 96-97 -0.15818024541465192 1101 98-99 -0.18907486520828343 1101 100-101 -0.31081213618259795 1102 1 0.9673443060173774 1102 2 0.9226405121971943 1102 3 0.7529495378715154 1102 4 0.9013102554350425 1102 5 0.9171905448658606 1102 6 0.8861939915200949 1102 7 0.9050531941730924 1102 8 1.0306458544142245 1102 9 1.5006626138801664 1102 10-11 1.2992115273016012 1102 12-13 1.3489012103768019 1102 14-15 1.772830637607413 1102 16-17 1.597303644425864 1102 18-19 1.3485680784346314 1102 20-21 1.378714941969207 1102 22-23 0.988634720903427 1102 24-25 0.7133580256858565 1102 26-27 1.0359990031879178 1102 28-29 1.194250732785541 1102 30-31 1.4519422441216108 1102 32-33 1.4348531728234661 1102 34-35 1.423949842459301 1102 36-37 1.5944903462488895 1102 38-39 1.3552649854493737 1102 40-41 1.496368850888473 1102 42-43 1.5264950187782205 1102 44-45 1.6571295671851232 1102 46-47 1.58410944360174 1102 48-49 1.3779610598754672 1102 50-51 1.2578046656246187 1102 52-53 1.223600153058193 1102 54-55 1.020929530033733 1102 56-57 1.2214281793485213 1102 58-59 1.2693852201926958 1102 60-61 1.5875847884851666 1102 62-63 1.5523180191877302 1102 64-65 1.4804808946863446 1102 66-67 1.2399555799146 1102 68-69 1.1109364330001696 1102 70-71 1.4291128697797717 1102 72-73 0.9200367309644726 1102 74-75 0.6964695261693059 1102 76-77 0.6596011673097877 1102 78-79 0.8740653415484729 1102 80-81 0.8365792142879407 1102 82-83 0.7543847166134832 1102 84-85 0.7438699620171789 1102 86-87 0.6719968316334182 1102 88-89 0.6361961193922561 1102 90-91 0.6395711422662451 1102 92-93 0.6862677433429774 1102 94-95 0.6632284121585172 1102 96-97 0.690921683091009 1102 98-99 0.6933356208275114 1102 100-101 0.7505600088574162 1103 1 0.6622145868606317 1103 2 0.4970084138719528 1103 3 0.3533477393281075 1103 4 0.642994332309506 1103 5 0.553104356472069 1103 6 0.6307617250936204 1103 7 0.5323680818136509 1103 8 0.6083694692588679 1103 9 0.9695407750514988 1103 10-11 0.7455141934229559 1103 12-13 0.9093305317302054 1103 14-15 1.2172639397130638 1103 16-17 1.0837036309870882 1103 18-19 0.9392786838826126 1103 20-21 0.8437678095751124 1103 22-23 0.7336909695658491 1103 24-25 0.4700543770966803 1103 26-27 0.8578971654157073 1103 28-29 0.7375088604298412 1103 30-31 1.1196016227762513 1103 32-33 1.1494755189077068 1103 34-35 0.9557629189721624 1103 36-37 1.185288536791326 1103 38-39 0.8810598638769918 1103 40-41 0.9359770833435874 1103 42-43 1.1659880700370593 1103 44-45 1.170697537296661 1103 46-47 1.1700991930384603 1103 48-49 0.9021499850024455 1103 50-51 0.7419068057693181 1103 52-53 0.29208300638249085 1103 54-55 0.5450989621366418 1103 56-57 0.7741878332262075 1103 58-59 0.8677870252612863 1103 60-61 1.0722035787310986 1103 62-63 0.9425490865334822 1103 64-65 0.7691421400104517 1103 66-67 0.6617315308343166 1103 68-69 0.4660756146485454 1103 70-71 0.9577645807574982 1103 72-73 0.45370067375412404 1103 74-75 0.33086363443069544 1103 76-77 0.31021907918015046 1103 78-79 0.30618769442979143 1103 80-81 0.32832485319110205 1103 82-83 0.25109228901489544 1103 84-85 0.22523416977601585 1103 86-87 0.2576554909639093 1103 88-89 0.166877143994153 1103 90-91 0.16498237467591537 1103 92-93 -0.14207695371940687 1103 94-95 -0.17054097745299757 1103 96-97 -0.09359619009431697 1103 98-99 -0.18732000215496925 1103 100-101 -0.26568794862322065 1104 1 0.5826657404432112 1104 2 0.46684774989570954 1104 3 0.24884610223071846 1104 4 0.6395975455890337 1104 5 0.4512315932039641 1104 6 0.6525568214158639 1104 7 0.5062480026238205 1104 8 0.6108734668222553 1104 9 0.9511417040090819 1104 10-11 0.7389431510107087 1104 12-13 0.8484010030619658 1104 14-15 1.2082012731479281 1104 16-17 1.091990960614737 1104 18-19 0.8293761482852631 1104 20-21 0.859105127781163 1104 22-23 0.5459079040058157 1104 24-25 0.5098487881223335 1104 26-27 0.1565690225694425 1104 28-29 0.6075922381805441 1104 30-31 0.8870085366573122 1104 32-33 1.0616012708303515 1104 34-35 0.8574900567167774 1104 36-37 1.09163742889519 1104 38-39 0.6796630010124503 1104 40-41 0.9664567909503745 1104 42-43 1.2388111112310014 1104 44-45 1.2463320077146918 1104 46-47 1.1189709283616835 1104 48-49 0.9041089814236756 1104 50-51 0.9624765537324578 1104 52-53 0.7759564549471705 1104 54-55 0.9210324883682688 1104 56-57 0.7894092220939442 1104 58-59 0.8078098684806605 1104 60-61 1.1345292088232313 1104 62-63 0.2676417919320997 1104 64-65 -0.5644729898151795 1104 66-67 0.08183546748245618 1104 68-69 0.5146250702184787 1104 70-71 1.0743032619423047 1104 72-73 0.61720215856338 1104 74-75 0.4872488853253891 1104 76-77 0.29952769107385535 1104 78-79 0.1525973114251471 1104 80-81 0.2409501101773195 1104 82-83 0.14486142021111803 1104 84-85 0.14879009759144068 1104 86-87 0.28896174239161 1104 88-89 0.44696337714728074 1104 90-91 0.6253583359947292 1104 92-93 0.7775741161047023 1104 94-95 0.7187647790382847 1104 96-97 0.761178994555042 1104 98-99 0.7021492737149728 1104 100-101 0.432265679641457 1105 1 -0.3353002291036624 1105 2 -1.718861294316362 1105 3 -0.3069482474298937 1105 4 0.488567000734875 1105 5 0.6089210079902472 1105 6 0.1265441631880151 1105 7 -0.11493022155522681 1105 8 -0.041306408429989006 1105 9 0.6454812747300522 1105 10-11 0.9999642939202644 1105 12-13 1.03883051140906 1105 14-15 1.3094159585267562 1105 16-17 1.026259902093372 1105 18-19 0.6606995293162683 1105 20-21 1.1004587784874502 1105 22-23 0.4689101054367413 1105 24-25 0.037547375537492655 1105 26-27 1.0115836987756452 1105 28-29 0.5662138213762873 1105 30-31 1.1358222864279952 1105 32-33 1.1931193409092344 1105 34-35 0.7546059528826206 1105 36-37 0.8853890341052448 1105 38-39 0.8072386752006722 1105 40-41 1.118859102452852 1105 42-43 1.4955871614970064 1105 44-45 1.5746004446987314 1105 46-47 1.7697123060905469 1105 48-49 1.5141222817355455 1105 50-51 1.236848687285935 1105 52-53 1.3347825877668384 1105 54-55 0.4021309106761066 1105 56-57 0.8146490863390383 1105 58-59 -0.01869719444356832 1105 60-61 0.6650176513108477 1105 62-63 1.31327263948301 1105 64-65 1.6486629026816146 1105 66-67 1.3203960526961822 1105 68-69 1.3176864351056636 1105 70-71 0.8032247901022735 1105 72-73 0.10036717415684748 1105 74-75 0.15426823957231406 1105 76-77 0.3283162161151303 1105 78-79 -0.026978913120807135 1105 80-81 0.18586113567722862 1105 82-83 0.1333584849698788 1105 84-85 0.30615090294826075 1105 86-87 -0.027803988666914137 1105 88-89 -0.3997748360246156 1105 90-91 -0.4988661182338525 1105 92-93 -0.13230686903563793 1105 94-95 -0.47821031727431773 1105 96-97 -0.4821238397872811 1105 98-99 -1.1507423975380071 1105 100-101 -0.5760722400063152 1106 1 0.39380952107088163 1106 2 0.3642307130903717 1106 3 -0.2469400514550273 1106 4 0.47625899903497526 1106 5 0.11400554795321227 1106 6 0.5489836799290515 1106 7 0.3780413505541702 1106 8 0.5173748743918551 1106 9 1.0262480537119316 1106 10-11 0.3169415349122815 1106 12-13 0.4880084475411621 1106 14-15 0.21783295910350375 1106 16-17 -0.04772205158419496 1106 18-19 0.5698659303726359 1106 20-21 0.5881505870659041 1106 22-23 0.6568567101782676 1106 24-25 0.5362143912919706 1106 26-27 0.8715554681955453 1106 28-29 0.8324230691679304 1106 30-31 0.48786767543325027 1106 32-33 0.4831323937580905 1106 34-35 -0.952218524244536 1106 36-37 -0.577747671719969 1106 38-39 -0.6434352160660595 1106 40-41 0.7153067544578278 1106 42-43 0.8701065965818486 1106 44-45 0.7870600339893272 1106 46-47 0.3797112057050285 1106 48-49 0.2609964279881609 1106 50-51 0.9663815167185916 1106 52-53 0.33417301213619766 1106 54-55 0.885258585751231 1106 56-57 0.5646380824838957 1106 58-59 0.9619580282137434 1106 60-61 1.1648237557943517 1106 62-63 1.0835658353060964 1106 64-65 1.0073883906456658 1106 66-67 0.7580123520617477 1106 68-69 0.8107373108607518 1106 70-71 1.2068202049096612 1106 72-73 0.44332508630813905 1106 74-75 0.5223270836363056 1106 76-77 0.65903347774886 1106 78-79 0.6326885427878821 1106 80-81 0.756537379492304 1106 82-83 0.6161962653784627 1106 84-85 0.6755774503179595 1106 86-87 0.6800064718254539 1106 88-89 0.7051199822979406 1106 90-91 0.9769929413539202 1106 92-93 0.5929187099948088 1106 94-95 0.7442073814780699 1106 96-97 1.0490191572075283 1106 98-99 1.0167840876029928 1106 100-101 0.9337462343281402 1107 1 0.3495921020159827 1107 2 0.3164421750270634 1107 3 0.12112422658584521 1107 4 -1.4299733863601034 1107 5 -0.0855946842378188 1107 6 0.19851154941878235 1107 7 0.43107283555237075 1107 8 0.46100577470993187 1107 9 0.7766526807222647 1107 10-11 0.7538236611937279 1107 12-13 0.9044929598218161 1107 14-15 1.0354667381700722 1107 16-17 0.9228771536080558 1107 18-19 0.7344821453962354 1107 20-21 0.14291903955473373 1107 22-23 0.5289748754489025 1107 24-25 0.326659231476782 1107 26-27 0.8100318065115317 1107 28-29 0.7542748976722677 1107 30-31 1.111657011981535 1107 32-33 1.123664305578032 1107 34-35 0.8528786466496356 1107 36-37 0.9291073480976948 1107 38-39 0.6532094397317607 1107 40-41 0.16708364619745453 1107 42-43 0.16646298354238098 1107 44-45 -0.097289791736074 1107 46-47 0.42053322497898904 1107 48-49 0.5965261203350494 1107 50-51 -1.0617854349336682 1107 52-53 0.42006228538354407 1107 54-55 0.8116637485870086 1107 56-57 0.7529422474761063 1107 58-59 0.9090408001123294 1107 60-61 1.2837662359832507 1107 62-63 1.4472557814871507 1107 64-65 1.240014644516613 1107 66-67 1.164863825975715 1107 68-69 1.0255865048899793 1107 70-71 1.394271514398767 1107 72-73 1.0323745477247677 1107 74-75 0.8149913968735554 1107 76-77 0.7922486142924186 1107 78-79 0.7037304606227295 1107 80-81 0.8538036190745686 1107 82-83 0.8984863150473785 1107 84-85 0.8938695855035661 1107 86-87 0.8559462019690507 1107 88-89 0.9505343408439622 1107 90-91 1.0242534024264671 1107 92-93 0.9883331998705955 1107 94-95 1.0205694681504625 1107 96-97 1.0588769008045453 1107 98-99 1.1091978236459248 1107 100-101 0.9326045846686775 1108 1 0.48685685693726555 1108 2 0.4716034164058307 1108 3 0.2519309824739402 1108 4 0.5357821366363211 1108 5 0.5407699919977489 1108 6 0.406661426305007 1108 7 0.5551614168661061 1108 8 0.536735122407201 1108 9 0.7870641648934793 1108 10-11 0.6664310393860191 1108 12-13 0.8735913285927737 1108 14-15 1.0006696346181343 1108 16-17 0.8536474724668324 1108 18-19 0.5690865197908082 1108 20-21 0.5992285922001699 1108 22-23 0.4621429189599624 1108 24-25 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1.689991640265763 2311 82-83 1.723114713550224 2311 84-85 1.9455915386205724 2311 86-87 1.6876499729562688 2311 88-89 1.9280951725582511 2311 90-91 2.0786685283381026 2311 92-93 2.103483572471827 2311 94-95 2.1713178126210657 2311 96-97 2.1386002945735925 2311 98-99 2.4510043358709552 2311 100-101 2.53398501911823 2312 1 -0.011148402354155706 2312 2 -0.20505684898595078 2312 3 -0.277249032616556 2312 4 0.14448095690175222 2312 5 -1.2886392653300192 2312 6 0.038987267610089305 2312 7 0.21414978663995043 2312 8 -2.884617232709868 2312 9 -4.9416478657729535 2312 10-11 -0.6979385295400888 2312 12-13 -5.719873750637298 2312 14-15 -2.2814152014231865 2312 16-17 -2.7974309458860773 2312 18-19 -2.4115948852268545 2312 20-21 -3.628466670309294 2312 22-23 -2.1293558945410993 2312 24-25 -2.9191337711229792 2312 26-27 -3.429028946162724 2312 28-29 -6.373673785631695 2312 30-31 -6.1452422146074746 2312 32-33 -1.512344295756435 2312 34-35 0.634252771160078 2312 36-37 0.9770428265900435 2312 38-39 0.8655462601702766 2312 40-41 -2.6340631983987777 2312 42-43 -5.214589740836402 2312 44-45 -3.480236994529591 2312 46-47 0.1564101615010216 2312 48-49 -1.4514012129542024 2312 50-51 -6.8838280793840525 2312 52-53 -1.6351966889533145 2312 54-55 0.7504329029903545 2312 56-57 1.1553809212837223 2312 58-59 1.3942553399706412 2312 60-61 1.595366250932102 2312 62-63 1.4778161043821711 2312 64-65 1.616852944986455 2312 66-67 1.2974346724731376 2312 68-69 1.5039509289426647 2312 70-71 1.770772507634419 2312 72-73 1.2610362716905392 2312 74-75 0.9463730437186335 2312 76-77 1.1990210152778964 2312 78-79 1.227693169008166 2312 80-81 1.165747649071914 2312 82-83 0.31690971037050275 2312 84-85 0.734703692631193 2312 86-87 1.0493497373704983 2312 88-89 1.160285298863588 2312 90-91 1.389370755637927 2312 92-93 1.5138147490690592 2312 94-95 1.606616351939497 2312 96-97 1.7558259952017004 2312 98-99 1.8309859544559686 2312 100-101 1.79269970147568 2313 1 -0.31010313827532343 2313 2 -0.1635870327129858 2313 3 -0.14770996184250862 2313 4 -1.5630569166535295 2313 5 -2.2440658896335606 2313 6 -0.7700747575315035 2313 7 -1.5358801438292673 2313 8 -2.6912203546880384 2313 9 -0.6453914767689497 2313 10-11 -1.263053416495012 2313 12-13 -4.833006319592499 2313 14-15 -3.037468063236517 2313 16-17 -5.39259141924796 2313 18-19 -4.642105545078579 2313 20-21 -4.404453224621591 2313 22-23 -3.0578220731108914 2313 24-25 -1.975946406385674 2313 26-27 -4.647332579261224 2313 28-29 -4.482413482643253 2313 30-31 -3.6047992436630523 2313 32-33 -7.701362115897339 2313 34-35 -3.560599188134482 2313 36-37 -3.8566634864366165 2313 38-39 -1.6562523307953327 2313 40-41 -1.3832228429169007 2313 42-43 -3.2081339688605297 2313 44-45 -5.439577603269289 2313 46-47 -2.861990170036485 2313 48-49 -0.617395595235358 2313 50-51 0.8321962333663251 2313 52-53 0.5929701849242903 2313 54-55 -2.906890852592827 2313 56-57 -5.8234283065101735 2313 58-59 -1.0394555777435954 2313 60-61 -6.35154630605091 2313 62-63 -1.1761968808367769 2313 64-65 -0.8553025092659823 2313 66-67 -3.457209154943172 2313 68-69 -4.414808568225453 2313 70-71 -4.538648683137794 2313 72-73 -0.30907930596751143 2313 74-75 0.8304868715954115 2313 76-77 -1.2681153295211125 2313 78-79 -0.35647591511947496 2313 80-81 0.8973173870651934 2313 82-83 1.0501417405772528 2313 84-85 1.2896882669278256 2313 86-87 0.882600186327533 2313 88-89 -4.142601840244023 2313 90-91 0.298796551097702 2313 92-93 1.6908235440223223 2313 94-95 1.2137075708002953 2313 96-97 1.9656984453559545 2313 98-99 2.585072614676072 2313 100-101 3.0319224302135375 2314 1 -28.741682085643745 2314 2 -14.690859759985713 2314 3 -5.123308047966908 2314 4 -3.8759755769406112 2314 5 -1.7507644542268608 2314 6 -1.1174431785841392 2314 7 -0.6411433017240071 2314 8 -5.320885426458375 2314 9 -3.900893869113446 2314 10-11 -1.1391299715189334 2314 12-13 0.035653967488840976 2314 14-15 -2.50253983357144 2314 16-17 -1.9803904623101545 2314 18-19 -4.211483535509203 2314 20-21 -2.9702426983058032 2314 22-23 -6.224329250144379 2314 24-25 -1.8060899470555327 2314 26-27 -2.4918301869167223 2314 28-29 -2.9238010424518635 2314 30-31 -3.596904506820948 2314 32-33 -1.5855726422131369 2314 34-35 -2.537154212057928 2314 36-37 -6.54183095055145 2314 38-39 -7.264625536536954 2314 40-41 -7.29279222090733 2314 42-43 -5.17990430378876 2314 44-45 -4.874745067384119 2314 46-47 -7.601463854247008 2314 48-49 -6.929596552173162 2314 50-51 -2.436703288164775 2314 52-53 -4.029517853353219 2314 54-55 -4.774115732975602 2314 56-57 -1.574385244309216 2314 58-59 -2.110747443772304 2314 60-61 -1.3733166409791409 2314 62-63 -0.8245222396884557 2314 64-65 -2.379225954242056 2314 66-67 -1.3454866669048968 2314 68-69 -0.012894692627369864 2314 70-71 0.43718863743637115 2314 72-73 0.1337436605396647 2314 74-75 0.3694820869064124 2314 76-77 -1.4028043247364224 2314 78-79 -4.160782135215172 2314 80-81 -2.351964909585522 2314 82-83 -2.390049646982554 2314 84-85 -0.5624648431200185 2314 86-87 -0.022184502667681016 2314 88-89 -0.25169274933493213 2314 90-91 0.04544726880105543 2314 92-93 -0.05558793923126615 2314 94-95 0.7134683363505374 2314 96-97 1.0118706941597857 2314 98-99 1.2341156768770318 2314 100-101 1.3032142962422455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 20.0 4 49.0 5 114.0 6 268.0 7 679.0 8 1760.0 9 2894.0 10 4597.0 11 6608.0 12 8721.0 13 10877.0 14 12912.0 15 16231.0 16 18389.0 17 20830.0 18 19177.0 19 16410.0 20 16155.0 21 16842.0 22 18643.0 23 21562.0 24 25901.0 25 31872.0 26 40216.0 27 51220.0 28 64291.0 29 80454.0 30 98050.0 31 119163.0 32 143877.0 33 172698.0 34 211171.0 35 259434.0 36 324561.0 37 396733.0 38 339441.0 39 72302.0 40 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.29488800236183 21.16634241981514 15.184404357272088 23.354365220550942 2 15.061712153105416 21.59600239529651 40.073507173792066 23.268778277806003 3 18.3681208108244 26.092630755963665 30.819894134766646 24.719354298445293 4 12.832279779054149 17.745342176107197 38.537214768183404 30.885163276655252 5 12.353440643224227 38.30103426088416 36.05963161591638 13.28589347997523 6 30.19086762962425 38.716323703757475 17.728861205874523 13.363947460743752 7 26.26622571435484 33.05800277031768 22.725262212382958 17.95050930294452 8 24.729016960634876 36.61605393046381 20.321195047301057 18.33373406160025 9 25.05288857032252 16.408385957271783 21.135883174856644 37.40284229754906 10-11 23.80352368682103 27.06341033364787 29.082249098739442 20.050816880791658 12-13 24.66680956787883 24.6205745989693 29.01062837216617 21.7019874609857 14-15 21.869650655682175 26.36117185042704 26.641492614502432 25.12768487938835 16-17 21.291051964627037 29.315597705112385 27.40130743267765 21.992042897582927 18-19 21.240904224432025 28.236542335051173 29.447667912704752 21.074885527812054 20-21 21.954313953207034 27.401250725847426 29.527019670465265 21.11741565048027 22-23 21.843527709225523 27.29243031864702 29.265998130942876 21.598043841184584 24-25 21.71400817089262 27.5048716617041 29.328566191546912 21.45255397585637 26-27 21.6178745722059 27.569033075686473 29.249982230381967 21.563110121725664 28-29 21.54526042304662 27.53621544617702 29.425380831182107 21.49314329959425 30-31 21.886488154016686 27.382749943669765 29.22711140078545 21.5036505015281 32-33 21.72851132393676 27.556557305467482 29.079855236888356 21.6350761337074 34-35 21.890072533406727 27.438560582747286 29.24238769722123 21.42897918662476 36-37 21.842491173458413 27.318670758184705 29.15412640582924 21.68471166252764 38-39 21.708432870437754 27.468611551432975 29.222890402512363 21.60006517561691 40-41 21.846939444822183 27.331404873849603 29.219828313800168 21.601827367528045 42-43 21.77139139274617 27.391715602306267 29.020494559647293 21.81639844530027 44-45 21.864235664738434 27.56527996652004 28.810401016647546 21.760083352093982 46-47 21.95244030929418 27.52286690865414 28.603339215358513 21.921353566693163 48-49 21.880158379197674 27.636576345855456 28.547088469222288 21.936176805724582 50-51 21.90189260363537 27.71894344134518 28.36824421473219 22.010919740287257 52-53 21.93904950066093 27.74092096600321 28.306296078496974 22.013733454838892 54-55 21.956518615051934 27.688107590106565 28.334754021733126 22.020619773108375 56-57 22.019381843641735 27.7787586062977 28.17807342931759 22.023786120742972 58-59 21.923278307994458 27.81053590634918 28.28960227133106 21.976583514325302 60-61 22.014669477415463 27.782766067164598 28.205493662961178 21.997070792458764 62-63 21.939543009360374 27.682321980335615 28.268862234021036 22.109272776282975 64-65 21.82934206447027 27.719381157984678 28.24113212398013 22.21014465356492 66-67 21.861358850372206 27.728173215426665 28.120777458100665 22.28969047610046 68-69 21.887032196880554 27.712465594615587 28.153145617593783 22.247356590910076 70-71 21.97155020584712 27.546425949950574 28.092654738831524 22.389369105370776 72-73 21.976870133626207 27.599057112018045 28.046399615888458 22.377673138467298 74-75 21.87001447173626 27.796205592411184 27.939429659347127 22.39435027650543 76-77 21.8186523130338 27.743155107546393 27.8631089557475 22.575083623672302 78-79 21.876614963879625 27.8100796806875 27.7074024746114 22.60590288082148 80-81 21.781999300613382 27.89724687402535 27.72574593363388 22.595007891727388 82-83 21.91623854301799 27.82866409354572 27.816509849190968 22.438587514245324 84-85 21.917826085723764 27.82928966148839 27.652723394321566 22.600160858466285 86-87 21.705437786067197 27.86443358931232 27.81328315827637 22.616845466344113 88-89 21.849300230956487 27.699221235445187 27.716158296494598 22.735320237103732 90-91 21.83063448743603 27.740107056593917 27.722659984734282 22.70659847123577 92-93 21.874422281476374 27.754457954945423 27.647845849481094 22.723273914097106 94-95 21.70433763521145 27.639436140501765 27.694139774021444 22.962086450265343 96-97 21.706579695505162 27.64625914119632 27.703023021600142 22.94413814169837 98-99 21.55284819326665 27.672086810001613 27.633695545743826 23.14136945098791 100-101 21.64282449492515 27.682154532356584 27.55043943468364 23.124581538034626 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 398.0 1 286.0 2 189.5 3 209.5 4 184.0 5 182.5 6 340.5 7 705.0 8 1167.5 9 1497.0 10 1511.5 11 1396.0 12 1475.0 13 1724.0 14 1950.5 15 2133.0 16 2314.5 17 2577.0 18 2885.5 19 3186.5 20 3607.5 21 4276.0 22 5237.5 23 6669.0 24 8626.0 25 11396.0 26 15242.5 27 19793.5 28 24832.0 29 30812.0 30 37309.0 31 43890.5 32 51171.5 33 58155.0 34 64882.0 35 71121.0 36 78937.0 37 85988.0 38 88010.0 39 89096.0 40 90178.5 41 90552.0 42 91659.0 43 93235.5 44 94653.0 45 97170.5 46 97622.5 47 95808.5 48 95512.5 49 94628.0 50 92408.5 51 89569.0 52 85975.5 53 82220.0 54 78390.0 55 72440.0 56 65551.5 57 58678.5 58 51608.5 59 44616.5 60 36933.0 61 29621.0 62 23212.0 63 17799.0 64 13223.0 65 9486.5 66 6781.0 67 5085.0 68 3869.5 69 2916.0 70 2193.0 71 1639.0 72 1262.0 73 951.0 74 696.0 75 516.0 76 378.0 77 265.0 78 194.0 79 141.5 80 93.0 81 66.0 82 57.5 83 47.5 84 32.0 85 22.0 86 16.5 87 11.0 88 11.0 89 8.5 90 5.5 91 4.0 92 2.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1196136072197624 2 0.0 3 0.004649960078391522 4 4.15850088311437E-4 5 3.78045534828579E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.020887015799278992 26-27 0.008317001766228738 28-29 0.006804819626914423 30-31 0.0025707096368343374 32-33 0.0013231593719000266 34-35 0.0021926641020057584 36-37 0.0013987684788657425 38-39 8.695047301057318E-4 40-41 0.0025518073600929083 42-43 0.0016255957997628897 44-45 7.371887929157291E-4 46-47 0.03667041687837217 48-49 0.019601660980861822 50-51 0.0855895090851903 52-53 0.0014176707556071714 54-55 0.035007016525126416 56-57 0.0010585274975200214 58-59 0.0014743775858314582 60-61 0.0037804553482857907 62-63 0.0036670416878372167 64-65 0.009224311049817328 66-67 0.005821901236360117 68-69 0.0025518073600929083 70-71 0.00523593065737582 72-73 0.004498741864460091 74-75 0.0010585274975200214 76-77 0.0 78-79 3.7804553482857906E-5 80-81 3.402409813457211E-4 82-83 6.615796859500133E-4 84-85 5.6706830224286856E-5 86-87 0.0017012049067286058 88-89 0.0036670416878372167 90-91 0.001549986692797174 92-93 0.00306216883211149 94-95 8.127978998814448E-4 96-97 6.048728557257265E-4 98-99 0.0019280322276257532 100-101 0.00306216883211149 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2645184.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.9850125272638 #Duplication Level Percentage of deduplicated Percentage of total 1 80.43256674088059 51.464787905152264 2 11.828153519502267 15.136491022395038 3 3.485834577560586 6.691235073395503 4 1.4913916957212672 3.817068653351299 5 0.7452284773034697 2.3841726727968116 6 0.46027000825914394 1.7670229346631081 7 0.29876902869545524 1.3381718030686 8 0.20281629553746353 1.0381762568558273 9 0.15874629853831557 0.9141645510567793 >10 0.8140277311569969 9.478786512593453 >50 0.058554155694967795 2.5762985842067807 >100 0.022925786106205052 2.473373194268903 >500 4.7712336219231084E-4 0.216486958066113 >1k 1.192808405470939E-4 0.19893293008836077 >5k 1.192808405470939E-4 0.5048309480412286 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7156 0.27052938472333116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6073 0.22958705330139603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3767 0.14240975296992572 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.7804553482857906E-5 0.0 0.0 0.0 3 0.0 3.7804553482857906E-5 0.0 0.0 0.0 4 0.0 7.560910696571581E-5 0.0 0.0 0.0 5 0.0 1.1341366044857371E-4 0.0 0.0 0.0 6 0.0 1.1341366044857371E-4 0.0 0.0 0.0 7 0.0 1.5121821393143163E-4 0.0 0.0 0.0 8 0.0 1.890227674142895E-4 0.0 0.0 0.0 9 0.0 1.890227674142895E-4 0.0 0.0 0.0 10-11 0.0 1.890227674142895E-4 0.0 3.7804553482857906E-5 0.0 12-13 7.560910696571581E-5 1.890227674142895E-4 0.0 7.560910696571581E-5 0.0 14-15 7.560910696571581E-5 2.457295976385764E-4 0.0 7.560910696571581E-5 1.8902276741428953E-5 16-17 7.560910696571581E-5 2.6463187438000534E-4 0.0 7.560910696571581E-5 3.7804553482857906E-5 18-19 1.1341366044857371E-4 2.6463187438000534E-4 0.0 7.560910696571581E-5 9.451138370714476E-5 20-21 1.5121821393143163E-4 2.6463187438000534E-4 0.0 9.451138370714476E-5 1.5121821393143163E-4 22-23 1.5121821393143163E-4 3.024364278628632E-4 0.0 2.0792504415571847E-4 1.5121821393143163E-4 24-25 1.890227674142895E-4 3.9694781157000795E-4 0.0 3.78045534828579E-4 1.5121821393143163E-4 26-27 2.2682732089714743E-4 4.1585008831143694E-4 0.0 8.127978998814449E-4 1.5121821393143163E-4 28-29 2.2682732089714743E-4 4.1585008831143694E-4 0.0 0.0022871754857129033 1.7012049067286058E-4 30-31 3.0243642786286325E-4 4.5365464179429485E-4 0.0 0.00793895623140016 1.890227674142895E-4 32-33 3.0243642786286325E-4 4.5365464179429485E-4 0.0 0.022777243473421888 1.890227674142895E-4 34-35 3.213387046042922E-4 5.103614720185817E-4 0.0 0.04634838256998379 1.890227674142895E-4 36-37 3.402409813457211E-4 5.670683022428685E-4 0.0 0.08464439524811884 1.890227674142895E-4 38-39 3.402409813457211E-4 5.670683022428685E-4 0.0 0.13702260409861847 1.890227674142895E-4 40-41 3.78045534828579E-4 6.804819626914422E-4 0.0 0.18996788125136096 1.890227674142895E-4 42-43 3.78045534828579E-4 7.182865161743002E-4 0.0 0.24478448380150492 1.890227674142895E-4 44-45 3.78045534828579E-4 7.938956231400159E-4 0.0 0.31511985555636207 1.890227674142895E-4 46-47 3.78045534828579E-4 8.317001766228739E-4 0.0 0.398535602816288 1.890227674142895E-4 48-49 4.5365464179429485E-4 8.317001766228739E-4 0.0 0.48697935568943407 1.890227674142895E-4 50-51 4.5365464179429485E-4 9.451138370714476E-4 0.0 0.5770865089158259 1.890227674142895E-4 52-53 4.5365464179429485E-4 9.451138370714476E-4 0.0 0.6622601679127047 2.0792504415571847E-4 54-55 4.5365464179429485E-4 9.829183905543054E-4 0.0 0.7591154339357867 2.2682732089714743E-4 56-57 4.5365464179429485E-4 9.829183905543054E-4 0.0 0.8733985991144662 2.457295976385764E-4 58-59 4.5365464179429485E-4 9.829183905543054E-4 0.0 0.9996658077472116 2.6463187438000534E-4 60-61 4.5365464179429485E-4 9.829183905543054E-4 0.0 1.1258385049962496 2.6463187438000534E-4 62-63 4.5365464179429485E-4 9.829183905543054E-4 0.0 1.2547331300960538 2.6463187438000534E-4 64-65 4.5365464179429485E-4 0.001209745711451453 0.0 1.3937593755292637 2.6463187438000534E-4 66-67 4.5365464179429485E-4 0.0012475502649343109 0.0 1.5509885134644699 2.6463187438000534E-4 68-69 4.5365464179429485E-4 0.0012853548184171687 0.0 1.725021775422806 2.6463187438000534E-4 70-71 4.914591952771527E-4 0.0012853548184171687 0.0 1.91251345842104 3.0243642786286325E-4 72-73 4.914591952771527E-4 0.0013042570951585977 0.0 2.106715449662481 3.0243642786286325E-4 74-75 4.914591952771527E-4 0.0013420616486414555 0.0 2.3029210822385133 3.0243642786286325E-4 76-77 4.914591952771527E-4 0.0013987684788657425 0.0 2.5204673852556194 3.0243642786286325E-4 78-79 4.914591952771527E-4 0.0014365730323486004 0.0 2.758636072197624 3.0243642786286325E-4 80-81 4.914591952771527E-4 0.0014365730323486004 0.0 3.0230978260869565 3.213387046042922E-4 82-83 4.914591952771527E-4 0.0014554753090900293 0.0 3.2972942524981246 3.402409813457211E-4 84-85 4.914591952771527E-4 0.0014743775858314582 0.0 3.565706582226416 3.402409813457211E-4 86-87 4.914591952771527E-4 0.001549986692797174 0.0 3.8553272664585903 3.591432580871501E-4 88-89 4.914591952771527E-4 0.0016255957997628899 0.0 4.170881874380005 3.78045534828579E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3865 0.0 53.25977 1 GTATCAA 6900 0.0 39.82352 1 ATCAACG 8350 0.0 31.847681 3 CAACGCA 8575 0.0 30.955479 5 TCAACGC 8630 0.0 30.813217 4 TATCAAC 8895 0.0 30.003134 2 AACGCAG 9030 0.0 29.606049 6 ACGCAGA 10335 0.0 25.592018 7 CGCAGAG 10360 0.0 25.209415 8 GTACATG 7000 0.0 21.664743 1 GCAGAGT 11650 0.0 21.439747 9 GAGTACT 7220 0.0 21.374994 12-13 ACATGGG 6760 0.0 21.144432 3 ACGTATA 250 9.536961E-9 20.89356 7 GTGGTAT 1855 0.0 20.502506 1 TACATGG 7260 0.0 20.342297 2 AGAGTAC 10420 0.0 20.028618 10-11 CAGAGTA 11455 0.0 19.338215 10-11 GTACTTT 7955 0.0 19.280672 14-15 TGGTATC 2000 0.0 19.2323 2 >>END_MODULE