FastQCFastQC Report
Sat 18 Jun 2016
SRR1931140_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931140_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805327
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48060.2662121599023335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35980.19929907435051933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.1192581731730595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.057.7622341
GTATCAA42800.047.3007771
CAACGCA52550.037.5582665
ATCAACG52800.037.2810443
TCAACGC54350.036.749114
TATCAAC58650.034.110822
AACGCAG60850.033.1157046
CGCAGAG63500.031.5135548
TAGCGCG650.00574461129.3345224
ACGCAGA69750.028.8958057
GCAGAGT73700.026.2506489
AATATCG1852.5131703E-723.1366755
GTACATG58000.022.638671
CAGAGTA70100.022.00319310-11
ACATGGG54150.021.3004533
TACATGG61300.021.1083852
AGAGTAC69350.020.69900510-11
GAGTACT47250.020.22402812-13
GTACTTT48350.020.05166414-15
TATACGA1455.936831E-419.7573832
Produced by FastQC (version 0.11.5)