##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931140_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1805327 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.7141670179419 31.0 30.0 33.0 26.0 34.0 2 29.483814289599614 31.0 30.0 34.0 25.0 34.0 3 29.50224862310263 31.0 30.0 34.0 25.0 34.0 4 32.75124506529842 35.0 33.0 37.0 25.0 37.0 5 32.87125988809783 35.0 33.0 37.0 26.0 37.0 6 33.16777902285846 35.0 33.0 37.0 27.0 37.0 7 32.92074565992753 35.0 33.0 37.0 26.0 37.0 8 33.083889511429234 35.0 33.0 37.0 26.0 37.0 9 34.206341565821596 37.0 34.0 39.0 25.0 39.0 10-11 34.20599979948231 37.0 33.0 39.0 26.0 39.0 12-13 34.238597218121704 37.0 33.5 39.0 26.5 39.0 14-15 35.118344211325706 38.0 34.0 40.0 26.0 41.0 16-17 35.1286492696337 38.0 34.0 40.0 26.0 41.0 18-19 35.125185908148495 38.5 33.5 40.0 25.5 41.0 20-21 35.310454837267706 39.0 34.0 40.0 27.0 41.0 22-23 35.21263073116394 38.5 34.0 40.0 27.0 41.0 24-25 35.108182063415654 38.0 34.0 40.0 26.0 41.0 26-27 34.77760815630631 38.0 33.0 40.0 24.5 41.0 28-29 34.755331028672366 38.0 33.0 40.0 25.0 41.0 30-31 34.481986642863035 38.0 33.0 40.0 24.5 41.0 32-33 34.26079208918938 38.0 33.0 40.0 24.0 41.0 34-35 34.29523598771857 38.0 33.0 40.0 24.0 41.0 36-37 33.94933743305229 38.0 32.5 40.0 23.5 41.0 38-39 33.76245937716547 38.0 32.0 40.0 22.5 41.0 40-41 33.64951224902747 38.0 32.5 40.0 21.0 41.0 42-43 33.910158658237535 38.0 33.0 40.0 22.5 41.0 44-45 33.898402339299196 38.0 33.0 40.0 22.5 41.0 46-47 33.62742151421875 38.0 32.0 40.0 20.5 41.0 48-49 33.358516767322484 38.0 32.0 40.0 18.5 41.0 50-51 32.84819979981466 37.5 31.5 39.5 15.0 40.5 52-53 32.77634910462204 37.0 31.0 40.0 14.5 40.5 54-55 32.74889590639258 37.0 31.0 40.0 14.0 41.0 56-57 32.507048861508196 37.0 31.0 40.0 11.5 41.0 58-59 32.29962217371147 36.0 31.0 40.0 9.5 41.0 60-61 32.10081691571665 36.0 31.0 40.0 8.0 41.0 62-63 31.926638221219754 36.0 30.5 39.0 8.5 41.0 64-65 31.603928540369694 35.0 30.0 39.0 7.5 41.0 66-67 31.362942004412496 35.0 30.0 39.0 7.0 40.0 68-69 31.157031662407974 35.0 30.0 39.0 3.5 40.0 70-71 30.880536046932217 35.0 30.0 38.0 2.0 40.0 72-73 30.543899526235414 35.0 30.0 37.5 2.0 40.0 74-75 30.148219408450657 34.0 29.0 37.0 2.0 39.5 76-77 29.859791051704207 34.0 29.0 36.0 2.0 39.0 78-79 29.459425910098282 34.0 29.0 36.0 2.0 39.0 80-81 29.066764082074883 34.0 29.0 35.5 2.0 37.5 82-83 28.75873650590724 34.0 29.0 35.0 2.0 37.0 84-85 28.442143722439205 34.0 28.0 35.0 2.0 37.0 86-87 28.1345518014188 33.5 28.0 35.0 2.0 36.0 88-89 27.81854976965392 33.0 27.0 35.0 2.0 36.0 90-91 27.47550443770021 33.0 27.0 35.0 2.0 36.0 92-93 27.137669519150826 33.0 26.0 35.0 2.0 35.0 94-95 26.86070224397021 33.0 26.0 35.0 2.0 35.0 96-97 26.488183858104378 33.0 25.0 35.0 2.0 35.0 98-99 26.1327745610629 33.0 25.0 35.0 2.0 35.0 100-101 25.528720004741523 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.296876671829594 1101 2 6.082401893496879 1101 3 5.624155608130131 1101 4 7.05530232215283 1101 5 6.342993686794614 1101 6 5.9530173094684 1101 7 6.408104184440912 1101 8 5.86080644160101 1101 9 6.68018861069654 1101 10-11 6.757757807655246 1101 12-13 6.58440516342521 1101 14-15 6.806324006223132 1101 16-17 6.67472805552357 1101 18-19 6.77224612180262 1101 20-21 6.151353298367418 1101 22-23 6.194752429137388 1101 24-25 6.008487169594051 1101 26-27 6.330304933553773 1101 28-29 6.221204530892745 1101 30-31 6.245053936231393 1101 32-33 6.096077096878684 1101 34-35 5.984860463036153 1101 36-37 6.200767459517191 1101 38-39 6.237281290009786 1101 40-41 6.32718704810847 1101 42-43 6.247824750559786 1101 44-45 6.103455762763968 1101 46-47 5.8629026530457296 1101 48-49 5.865431306174873 1101 50-51 5.863730912770222 1101 52-53 5.476739755248442 1101 54-55 5.937661155987584 1101 56-57 5.985349713113756 1101 58-59 5.803014052427876 1101 60-61 5.6321924482444246 1101 62-63 5.473200740617294 1101 64-65 5.445605555403166 1101 66-67 5.361053289285547 1101 68-69 5.533727706063768 1101 70-71 5.258606681858669 1101 72-73 5.125613082692812 1101 74-75 5.38422094094236 1101 76-77 5.26910138169027 1101 78-79 5.29974829077409 1101 80-81 5.190683380553224 1101 82-83 4.9645925570719385 1101 84-85 4.8984545326973965 1101 86-87 4.840170744891868 1101 88-89 4.8694988792041976 1101 90-91 4.9200905891912825 1101 92-93 4.804316568914992 1101 94-95 4.717007631259577 1101 96-97 4.80250220877836 1101 98-99 4.612876828934457 1101 100-101 4.677018392229844 1102 1 5.303967284140565 1102 2 5.961212705279941 1102 3 5.664766615068487 1102 4 7.004680658116715 1102 5 6.453651144289886 1102 6 5.974933338732697 1102 7 6.466317928949355 1102 8 5.965596919461678 1102 9 6.749739168957589 1102 10-11 6.890663283245097 1102 12-13 6.920942416052384 1102 14-15 7.123176430150789 1102 16-17 7.048406867684346 1102 18-19 7.050372630996055 1102 20-21 6.329314645028866 1102 22-23 6.474824016055692 1102 24-25 6.362119845964532 1102 26-27 6.61453872419801 1102 28-29 6.403749463824983 1102 30-31 6.677525708412325 1102 32-33 6.353326936122887 1102 34-35 6.330164415155661 1102 36-37 6.575511711654226 1102 38-39 6.370760360279181 1102 40-41 6.5736429110860755 1102 42-43 6.478971670118938 1102 44-45 6.421774999050253 1102 46-47 6.498128857814891 1102 48-49 6.226914936063313 1102 50-51 6.166712112210831 1102 52-53 5.825228605765783 1102 54-55 6.127544422545434 1102 56-57 6.055966790550833 1102 58-59 6.1697558134121095 1102 60-61 6.025466560490202 1102 62-63 5.869855581053109 1102 64-65 5.8411423898404315 1102 66-67 5.737715279419856 1102 68-69 5.813819483011169 1102 70-71 5.567005958794827 1102 72-73 5.504148383767067 1102 74-75 5.572556185358625 1102 76-77 5.349268387855197 1102 78-79 5.396694919848635 1102 80-81 5.387305305395955 1102 82-83 5.256699199996209 1102 84-85 5.245479358788845 1102 86-87 5.28622267238763 1102 88-89 5.378984775412157 1102 90-91 5.31824376361245 1102 92-93 5.457962375549371 1102 94-95 5.366848943926232 1102 96-97 5.107193670059818 1102 98-99 4.964171418995921 1102 100-101 4.918621498526527 1103 1 4.926510910286673 1103 2 5.659438082387307 1103 3 5.331823698771533 1103 4 6.743456168320794 1103 5 6.1974724989186925 1103 6 5.649905240536896 1103 7 6.169737035722502 1103 8 5.499900379976314 1103 9 6.202912786230584 1103 10-11 6.369707944587894 1103 12-13 6.4392417357802785 1103 14-15 6.642200387559836 1103 16-17 6.438815030949652 1103 18-19 6.385817765571627 1103 20-21 5.726750314949015 1103 22-23 6.127695947436941 1103 24-25 5.84983649784267 1103 26-27 5.918383740465384 1103 28-29 5.827570818453783 1103 30-31 6.028220769045269 1103 32-33 5.939615016572457 1103 34-35 5.966387426099164 1103 36-37 5.920306151169168 1103 38-39 5.741174438954129 1103 40-41 5.88995761087903 1103 42-43 5.8847953909115915 1103 44-45 5.770849237441265 1103 46-47 5.752447106853637 1103 48-49 5.604501448929323 1103 50-51 5.397992798398349 1103 52-53 5.13177400653478 1103 54-55 5.45661570323162 1103 56-57 5.4204565864692 1103 58-59 5.640977345503202 1103 60-61 5.371325773740708 1103 62-63 5.426069718882154 1103 64-65 5.3432867258357 1103 66-67 5.52165789994633 1103 68-69 5.39160416210019 1103 70-71 5.299667893133776 1103 72-73 5.159933654397058 1103 74-75 5.282840125021448 1103 76-77 5.228653185252419 1103 78-79 5.292817368828224 1103 80-81 5.302691285875106 1103 82-83 5.101165334276008 1103 84-85 5.140915620249952 1103 86-87 5.158168842159885 1103 88-89 5.153453867396777 1103 90-91 5.096365621051078 1103 92-93 5.045424664812899 1103 94-95 4.956650777703224 1103 96-97 4.897208458583922 1103 98-99 4.795419570430408 1103 100-101 4.847172223164208 1104 1 4.674995010336374 1104 2 5.614925539584441 1104 3 5.141177364038626 1104 4 6.6612557579011344 1104 5 6.207147547781801 1104 6 5.940203663805804 1104 7 6.280056376367771 1104 8 5.612432205794942 1104 9 6.431350660708034 1104 10-11 6.527088332870505 1104 12-13 6.464906685758471 1104 14-15 6.552158095430084 1104 16-17 6.497335812637498 1104 18-19 6.580377113290989 1104 20-21 5.974281241259469 1104 22-23 6.222301337777743 1104 24-25 5.8644137137581716 1104 26-27 6.152179329521271 1104 28-29 6.0204073793443875 1104 30-31 6.212686110538208 1104 32-33 6.174262241665989 1104 34-35 6.001933854441905 1104 36-37 6.162915395673782 1104 38-39 6.067782195458793 1104 40-41 6.327120104742555 1104 42-43 6.313410422760285 1104 44-45 6.213333770474559 1104 46-47 6.14866459878094 1104 48-49 5.853266988314058 1104 50-51 5.782303241535715 1104 52-53 5.650547809197942 1104 54-55 5.872986142039153 1104 56-57 5.881864636914841 1104 58-59 5.7835484752549675 1104 60-61 5.663994019882736 1104 62-63 5.630011394162757 1104 64-65 5.639506352189162 1104 66-67 5.65810353153827 1104 68-69 5.56107546474048 1104 70-71 5.483687139559368 1104 72-73 5.421810973679769 1104 74-75 5.486211603599433 1104 76-77 5.596524583027126 1104 78-79 5.544845226138786 1104 80-81 5.499956476128702 1104 82-83 5.328335664532716 1104 84-85 5.316533765311657 1104 86-87 5.19247974267428 1104 88-89 5.292192177602079 1104 90-91 5.298979073424409 1104 92-93 5.279123289937537 1104 94-95 5.151953210644127 1104 96-97 5.130017942754105 1104 98-99 5.055276197976095 1104 100-101 5.122395804304421 1105 1 4.498985764081429 1105 2 5.248785225047879 1105 3 4.970367941144243 1105 4 6.3200134090519136 1105 5 3.877465180084968 1105 6 4.973415519589722 1105 7 5.593186194970908 1105 8 0.8176272489646195 1105 9 0.8067590824938691 1105 10-11 2.3375267332820613 1105 12-13 5.61927384539181 1105 14-15 3.7174932323370236 1105 16-17 5.880987787425408 1105 18-19 6.366305028012388 1105 20-21 3.4234293998616536 1105 22-23 5.515296878542536 1105 24-25 5.71496150087123 1105 26-27 5.899749171524579 1105 28-29 3.5527077551584476 1105 30-31 5.71511222813546 1105 32-33 5.9132992722114075 1105 34-35 5.673915619151803 1105 36-37 5.928279782796405 1105 38-39 5.804727835583261 1105 40-41 5.958386991905009 1105 42-43 3.287701930182095 1105 44-45 5.354508798302103 1105 46-47 5.613892807370249 1105 48-49 5.466935431997722 1105 50-51 5.659432247506402 1105 52-53 5.444301920731174 1105 54-55 5.593978577956136 1105 56-57 5.525587335765088 1105 58-59 5.428706824768867 1105 60-61 5.427582793718244 1105 62-63 5.280385899356084 1105 64-65 5.387795840549391 1105 66-67 5.481301171945002 1105 68-69 5.472525103153185 1105 70-71 5.156919607071146 1105 72-73 5.139819261620257 1105 74-75 5.305684223348905 1105 76-77 5.25660492437828 1105 78-79 5.147649604793642 1105 80-81 5.09191767463637 1105 82-83 4.9416816160524775 1105 84-85 4.896564859315248 1105 86-87 4.937548748144188 1105 88-89 5.019397382091817 1105 90-91 5.077307110609741 1105 92-93 5.048402198141936 1105 94-95 5.0756327398180865 1105 96-97 5.09884398477881 1105 98-99 4.974595225639742 1105 100-101 5.134154504820909 1106 1 4.238673558087633 1106 2 4.504455255491738 1106 3 4.568492717311681 1106 4 6.237431618465049 1106 5 6.015917023939373 1106 6 5.189133574443353 1106 7 5.869535330116076 1106 8 5.344742345047624 1106 9 6.053746644634522 1106 10-11 6.0584155503575445 1106 12-13 6.2774625278916645 1106 14-15 6.121337196654235 1106 16-17 6.157628529395172 1106 18-19 6.297745739270095 1106 20-21 5.619803522740405 1106 22-23 5.714656947497424 1106 24-25 5.826275898254817 1106 26-27 5.889332970490994 1106 28-29 5.8616782673714845 1106 30-31 6.0933341022759535 1106 32-33 6.0067836477126555 1106 34-35 5.381309535008118 1106 36-37 5.683636579128628 1106 38-39 5.672581299078242 1106 40-41 5.60903925510528 1106 42-43 2.3306119796001212 1106 44-45 4.811804240979139 1106 46-47 5.040324270826318 1106 48-49 5.032511930012742 1106 50-51 5.17786428365828 1106 52-53 4.902109384498569 1106 54-55 5.420760048485448 1106 56-57 5.289962579318583 1106 58-59 5.279367186697904 1106 60-61 5.092682807759552 1106 62-63 5.122690568799108 1106 64-65 5.228811221297452 1106 66-67 5.165293968809433 1106 68-69 5.085694911407895 1106 70-71 4.986844265228537 1106 72-73 4.959221883084098 1106 74-75 4.997363844475647 1106 76-77 4.8689793217723825 1106 78-79 4.896412313618573 1106 80-81 4.982704608126351 1106 82-83 5.121227408841769 1106 84-85 5.15132167920741 1106 86-87 5.020133694079917 1106 88-89 5.10921333585123 1106 90-91 5.087137008381696 1106 92-93 5.146192178647141 1106 94-95 5.074478079341514 1106 96-97 5.180988562338076 1106 98-99 5.067760582223087 1106 100-101 5.086107876127112 1107 1 3.0062436618752635 1107 2 -0.11756510326801362 1107 3 1.3107335678978025 1107 4 3.254897587868399 1107 5 2.9533169801617554 1107 6 4.228255594802114 1107 7 4.9797749583964155 1107 8 4.419168413969199 1107 9 5.163792524445455 1107 10-11 2.6673456427023083 1107 12-13 5.176777364820033 1107 14-15 3.9442056925896303 1107 16-17 2.209540441025066 1107 18-19 3.016778058855376 1107 20-21 2.9602792900948742 1107 22-23 4.555035407175417 1107 24-25 4.393774050709286 1107 26-27 3.6545784177270413 1107 28-29 4.344986424962247 1107 30-31 3.8584728144771248 1107 32-33 4.352622606758903 1107 34-35 4.804549165455665 1107 36-37 4.877888707869499 1107 38-39 4.978132279415153 1107 40-41 4.004228159862954 1107 42-43 4.445460177457619 1107 44-45 4.8219836208540094 1107 46-47 4.998064240442048 1107 48-49 2.894339472202887 1107 50-51 4.38262921735253 1107 52-53 4.587241898482478 1107 54-55 5.008567223246999 1107 56-57 3.928150857752904 1107 58-59 3.045413525957109 1107 60-61 3.763484653354592 1107 62-63 4.418413690533399 1107 64-65 4.695836380424755 1107 66-67 4.729696263450137 1107 68-69 4.689395962287453 1107 70-71 4.577565360622568 1107 72-73 4.411134444876637 1107 74-75 4.557612033230871 1107 76-77 4.407576335792058 1107 78-79 4.4177620296058535 1107 80-81 4.451055882336785 1107 82-83 4.314219739089719 1107 84-85 4.310208427375574 1107 86-87 4.188844291514371 1107 88-89 4.285528669070423 1107 90-91 4.413248840691743 1107 92-93 4.431490484106558 1107 94-95 4.521585397355569 1107 96-97 4.612152591872796 1107 98-99 4.464291422688344 1107 100-101 4.595487555943677 1108 1 -1.440272950792778 1108 2 -0.7137537266266385 1108 3 -1.564287847538825 1108 4 -3.6554750978367494 1108 5 -8.698707111728552 1108 6 -6.93927822255181 1108 7 -5.555408034130071 1108 8 -5.722787567314661 1108 9 -7.046150710370526 1108 10-11 -2.462559253723118 1108 12-13 -1.0399367647995064 1108 14-15 -6.586659757553324 1108 16-17 -5.44625524238435 1108 18-19 -3.815040711346523 1108 20-21 -4.168249307603304 1108 22-23 -3.1482195488325146 1108 24-25 -8.254608845876046 1108 26-27 -9.163382566202817 1108 28-29 -5.696921205024697 1108 30-31 -5.7839129984147775 1108 32-33 -2.2723002024895145 1108 34-35 -0.6686843941515832 1108 36-37 -4.5431265932220235 1108 38-39 -5.764005875849616 1108 40-41 -3.186750896791821 1108 42-43 -3.8579183218247266 1108 44-45 -1.4339736777171552 1108 46-47 -2.1422468449493834 1108 48-49 -1.1063166967753801 1108 50-51 -0.46634817175358023 1108 52-53 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-6.447350439722918 2313 58-59 -5.888698292868089 2313 60-61 -3.7783176585114155 2313 62-63 -4.632624290122415 2313 64-65 -4.838004148001755 2313 66-67 -6.674667973398176 2313 68-69 -7.661075847101554 2313 70-71 -6.77143204442579 2313 72-73 -6.309684144413289 2313 74-75 -8.006719176960214 2313 76-77 -6.721263013110985 2313 78-79 -8.430374323307568 2313 80-81 -11.00203446916121 2313 82-83 -9.552093070535193 2313 84-85 -11.72671065731421 2313 86-87 -9.811120322781452 2313 88-89 -10.141659346971096 2313 90-91 -10.242094400638756 2313 92-93 -10.175515885320195 2313 94-95 -10.005260556878918 2313 96-97 -9.832999566655158 2313 98-99 -9.83220539259828 2313 100-101 -9.454760143985549 2314 1 -14.380380541946392 2314 2 -16.338424975879477 2314 3 -15.192769616815573 2314 4 -25.833000501303577 2314 5 -20.6848995803478 2314 6 -18.731298545325274 2314 7 -18.61353714351441 2314 8 -15.22541757329195 2314 9 -17.14018836727429 2314 10-11 -14.769119325450554 2314 12-13 -15.90307932307805 2314 14-15 -18.79305545390718 2314 16-17 -17.88775255260551 2314 18-19 -16.321040412482205 2314 20-21 -16.857714340478378 2314 22-23 -19.47513975206025 2314 24-25 -17.616974356934026 2314 26-27 -18.07368591348315 2314 28-29 -20.475634594146037 2314 30-31 -19.299648204631154 2314 32-33 -16.617361469674215 2314 34-35 -16.651103700786372 2314 36-37 -16.296952056461713 2314 38-39 -16.001087465807775 2314 40-41 -18.40349477007335 2314 42-43 -18.095702242924546 2314 44-45 -16.085580073413507 2314 46-47 -14.407668243634381 2314 48-49 -15.30199810741495 2314 50-51 -14.376367597934095 2314 52-53 -13.713793133364653 2314 54-55 -12.784267171657465 2314 56-57 -12.300591180463659 2314 58-59 -12.1248094039792 2314 60-61 -13.145910251104006 2314 62-63 -16.07521688271501 2314 64-65 -12.61809674059435 2314 66-67 -12.424205010435212 2314 68-69 -15.963390661916371 2314 70-71 -15.539950562944309 2314 72-73 -14.65088784811699 2314 74-75 -12.469219176960213 2314 76-77 -13.301818568666542 2314 78-79 -12.799355804789048 2314 80-81 -12.576571506198249 2314 82-83 -12.974315292757414 2314 84-85 -12.64846991657347 2314 86-87 -13.437509211670342 2314 88-89 -12.482863050674798 2314 90-91 -12.107372178416533 2314 92-93 -13.263015885320195 2314 94-95 -12.505723519841883 2314 96-97 -12.385777344432936 2314 98-99 -12.55720539259828 2314 100-101 -12.207537921763327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39599.0 3 6672.0 4 4725.0 5 3933.0 6 4191.0 7 4639.0 8 5413.0 9 6264.0 10 7225.0 11 8086.0 12 9167.0 13 9989.0 14 11376.0 15 12617.0 16 14278.0 17 15597.0 18 13714.0 19 11951.0 20 11735.0 21 12618.0 22 14607.0 23 17918.0 24 22410.0 25 27763.0 26 32772.0 27 38390.0 28 43533.0 29 49975.0 30 57842.0 31 67386.0 32 81652.0 33 102051.0 34 129565.0 35 164935.0 36 211622.0 37 254187.0 38 220563.0 39 54309.0 40 58.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84239733260347 19.854441173113724 13.819667062993862 23.483494431288943 2 15.10246244005979 20.115376054556343 40.363079884568435 24.419081620815426 3 18.260071444120648 25.65175173251162 30.070674177032746 26.017502646334982 4 11.963109134549864 17.229235677115927 39.1844181706425 31.623237017691707 5 13.257376463249217 37.16847262644493 35.074968975675596 14.49918193463025 6 30.373265693653234 37.26286609283823 18.46635341732851 13.897514796180023 7 26.80408255377523 32.18235421648935 22.597657083891747 18.41590614584367 8 25.604035367688887 34.811478418634465 20.778406324752392 18.806079888924256 9 25.623034303483582 15.977591614211125 21.251480510493327 37.14789357181197 10-11 24.28283368363953 26.217278216519457 28.79977303819054 20.70011506165047 12-13 24.992120863730893 24.0058971450893 28.432695530199286 22.56928646098052 14-15 22.53372296111569 25.341582839297395 26.376985675106663 25.747708524480256 16-17 21.879433558144854 28.268415252063857 27.034303973826674 22.81784721596462 18-19 21.673150499060014 27.426888387292543 28.958766598120917 21.941194515526526 20-21 22.659546391569666 26.649606172605406 28.95463028573139 21.736217150093537 22-23 22.220956280949146 26.569242783233797 28.812148822577594 22.397652113239463 24-25 22.126590840418366 26.69970089012217 28.81536964603021 22.35833862342926 26-27 21.91890401596757 26.893901991175234 28.735741856174286 22.45145213668291 28-29 21.820208787970113 26.85775297166449 28.991739808257535 22.330298432107867 30-31 22.11396730577057 26.693632732826323 28.90047658288849 22.291923378514618 32-33 21.92600124974287 26.937974717934587 28.714755338159687 22.42126869416286 34-35 22.209138280043387 27.081874447587857 28.770220676431187 21.938766595937576 36-37 22.193513570176478 26.568826767026472 28.638428778585666 22.599230884211387 38-39 22.229577023440772 26.841111336038004 28.75783045996933 22.171481180551897 40-41 22.132919169246875 26.687079145115643 28.86684683765537 22.313154847982116 42-43 22.063264369141777 26.88228327071255 28.62067550603067 22.433776854115006 44-45 22.067507858453588 27.11687613303101 28.46035292356649 22.355263084948913 46-47 22.21579721646944 26.76382347225853 28.39257167076977 22.62780764050226 48-49 22.305113952488448 27.102481697271248 28.191843701391285 22.400560648849027 50-51 22.131050526883577 27.150643217342353 27.951064979212646 22.76724127656142 52-53 22.125850429709036 27.287320845995815 27.8200530514567 22.766775672838445 54-55 22.14687601997236 27.403301411815363 27.76588726310599 22.683935305106285 56-57 22.15376477086202 27.334999205404582 27.5699049486069 22.9413310751265 58-59 22.217887523458778 27.490992996127112 27.70758900164663 22.58353047876748 60-61 22.423453614467356 27.462051705825097 27.806548235755574 22.307946443951977 62-63 22.12898272722892 27.42841047632922 27.83271396262284 22.609892833819025 64-65 22.145406344667755 27.309761611054395 27.83074755986035 22.714084484417505 66-67 22.280451131567855 27.329148680543746 27.57026843336415 22.82013175452425 68-69 22.16313166534373 27.26738701631339 27.659227386506714 22.910253931836174 70-71 22.299118109904743 27.093568090434587 27.62388752840898 22.983426271251687 72-73 22.26483069272215 27.10655742699245 27.746469199208786 22.882142681076616 74-75 22.095027659809 27.563372175788654 27.575558333753385 22.766041830648962 76-77 22.25627268633328 27.227837394555117 27.502081340388752 23.01380857872286 78-79 22.09400291470742 27.37490216453861 27.469704934341532 23.061389986412433 80-81 22.142692154939244 27.360832691252057 27.53725502360514 22.95922013020356 82-83 22.37974062316688 27.089386022587597 27.69938631616322 22.831487038082297 84-85 22.338889298171466 27.217202202149526 27.410574372399015 23.03333412727999 86-87 22.173849945189986 27.475216401239223 27.533072955758154 22.817860697812637 88-89 22.376998737624817 26.961237493262995 27.60253405615714 23.059229712955048 90-91 22.324764433257798 27.162475274562446 27.57890952719369 22.933850764986065 92-93 22.341104963255965 27.21008437806558 27.58417173176937 22.864638926909088 94-95 22.181632468799283 26.982757140396174 27.63582442405171 23.199785966752838 96-97 22.321219369122602 26.915650184149463 27.50717737008309 23.255953076644843 98-99 21.965632818874365 27.119989896567215 27.60040147851331 23.31397580604511 100-101 22.33265774012132 26.897370947202365 27.48867656662754 23.28129474604878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 468.0 1 302.5 2 178.5 3 221.5 4 194.5 5 188.5 6 303.0 7 549.0 8 940.0 9 1160.5 10 1058.0 11 965.5 12 1009.5 13 1104.5 14 1221.5 15 1353.0 16 1499.0 17 1716.5 18 1894.5 19 2087.0 20 2436.0 21 2894.0 22 3402.0 23 4103.5 24 5249.5 25 6836.0 26 8933.0 27 11459.0 28 14060.0 29 17713.5 30 22347.5 31 26770.5 32 31989.5 33 37526.0 34 42901.5 35 47472.0 36 52438.5 37 57566.0 38 58925.5 39 59206.5 40 58711.5 41 57195.0 42 56525.0 43 57739.0 44 60268.5 45 61848.5 46 62116.5 47 61687.5 48 61217.5 49 60034.0 50 58421.5 51 57872.0 52 57819.0 53 57228.0 54 56611.0 55 54602.0 56 50686.0 57 45593.5 58 41485.5 59 38635.5 60 34354.0 61 31371.5 62 26728.0 63 18636.5 64 12334.5 65 8652.0 66 6427.0 67 4511.5 68 3309.0 69 2502.0 70 1869.5 71 1372.0 72 1069.0 73 839.0 74 608.0 75 443.0 76 331.0 77 266.0 78 206.5 79 157.5 80 121.0 81 96.0 82 76.5 83 66.5 84 56.5 85 45.0 86 35.0 87 25.0 88 22.0 89 23.5 90 16.5 91 8.5 92 9.0 93 8.5 94 7.5 95 4.5 96 1.5 97 2.5 98 3.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023319875014332584 2 0.20716468540048424 3 0.0 4 0.4205332330375605 5 0.19470156930018773 6 0.09150696798973261 7 0.10236372690376869 8 0.10374851758157941 9 0.10496713337805284 10-11 0.13185976834113708 12-13 0.17165865242141728 14-15 0.11452218905494682 16-17 0.10881685146236664 18-19 0.14612311232258754 20-21 0.0966583893111885 22-23 0.09208858007441312 24-25 0.09095305171860832 26-27 0.14806181927152254 28-29 0.10626883661519493 30-31 0.1148268430040652 32-33 0.09726769720942523 34-35 0.13507248271365796 36-37 0.13003184464642695 38-39 0.12584977679943854 40-41 0.10275146829355568 42-43 0.10408086734425398 44-45 0.08513693087180328 46-47 0.08920821546456681 48-49 0.09430424515891027 50-51 0.08117642953326461 52-53 0.08898664895611709 54-55 0.2957635929668143 56-57 0.3140151341003597 58-59 0.3264782502006562 60-61 0.15739530843996682 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1805327.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.4993596138005 #Duplication Level Percentage of deduplicated Percentage of total 1 83.46009443765301 52.162024556606255 2 10.18898967113781 12.736106591154817 3 2.695247189142741 5.053536699669515 4 1.0752477064014525 2.6880917230519423 5 0.5856926483601551 1.830270772651027 6 0.35417382436688993 1.3281382328940763 7 0.24029621910758367 1.051285186828903 8 0.18199893590645022 0.9099853555637009 9 0.14641713525800676 0.8235879471101368 >10 0.9300408269288044 11.39456391236512 >50 0.0933361783430544 3.9775583613824455 >100 0.04698839908911905 4.9756609986272275 >500 0.0011999229977430967 0.4601772922483014 >1k 2.7690530717148387E-4 0.6090123698464733 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4806 0.2662121599023335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3598 0.19929907435051933 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2153 0.1192581731730595 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.1078325422485788E-4 0.0 0.0 0.0 0.0 7 1.1078325422485788E-4 0.0 0.0 0.0 0.0 8 1.1078325422485788E-4 0.0 0.0 0.0 0.0 9 1.1078325422485788E-4 0.0 0.0 5.539162711242894E-5 0.0 10-11 1.1078325422485788E-4 0.0 0.0 8.308744066864341E-5 0.0 12-13 1.1078325422485788E-4 0.0 0.0 1.1078325422485788E-4 0.0 14-15 1.1078325422485788E-4 0.0 0.0 1.1078325422485788E-4 0.0 16-17 1.6617488133728681E-4 0.0 0.0 1.1078325422485788E-4 0.0 18-19 1.6617488133728681E-4 0.0 0.0 1.1078325422485788E-4 5.539162711242894E-5 20-21 1.6617488133728681E-4 0.0 0.0 1.1078325422485788E-4 5.539162711242894E-5 22-23 1.6617488133728681E-4 0.0 0.0 1.3847906778107234E-4 5.539162711242894E-5 24-25 1.6617488133728681E-4 0.0 0.0 2.492623220059302E-4 5.539162711242894E-5 26-27 1.6617488133728681E-4 0.0 0.0 6.093078982367183E-4 5.539162711242894E-5 28-29 1.6617488133728681E-4 0.0 0.0 0.002132577643828514 5.539162711242894E-5 30-31 1.6617488133728681E-4 0.0 0.0 0.006176166423035826 5.539162711242894E-5 32-33 1.6617488133728681E-4 0.0 0.0 0.017503754167527544 5.539162711242894E-5 34-35 1.6617488133728681E-4 0.0 0.0 0.03353963021657572 5.539162711242894E-5 36-37 1.6617488133728681E-4 0.0 0.0 0.05763498801048231 5.539162711242894E-5 38-39 1.6617488133728681E-4 0.0 0.0 0.09408267865046055 5.539162711242894E-5 40-41 1.6617488133728681E-4 0.0 0.0 0.13368769203584724 5.539162711242894E-5 42-43 1.6617488133728681E-4 0.0 0.0 0.1740127965736955 5.539162711242894E-5 44-45 1.6617488133728681E-4 0.0 0.0 0.22580396792381657 5.539162711242894E-5 46-47 1.6617488133728681E-4 0.0 0.0 0.29058447583180225 5.539162711242894E-5 48-49 1.6617488133728681E-4 0.0 0.0 0.35445102189243277 5.539162711242894E-5 50-51 1.6617488133728681E-4 0.0 0.0 0.42646013713859043 5.539162711242894E-5 52-53 1.6617488133728681E-4 0.0 0.0 0.4985800356389729 8.308744066864341E-5 54-55 1.6617488133728681E-4 0.0 0.0 0.5766268382403852 1.1078325422485788E-4 56-57 1.6617488133728681E-4 0.0 0.0 0.6667213197387509 1.1078325422485788E-4 58-59 1.6617488133728681E-4 0.0 0.0 0.7704975331338866 1.1078325422485788E-4 60-61 1.938706948935013E-4 0.0 0.0 0.8803391296978331 1.1078325422485788E-4 62-63 2.2156650844971576E-4 0.0 0.0 0.9916763001938154 1.1078325422485788E-4 64-65 2.2156650844971576E-4 0.0 0.0 1.106447751570768 1.1078325422485788E-4 66-67 2.2156650844971576E-4 0.0 0.0 1.241769496606432 1.1078325422485788E-4 68-69 2.2156650844971576E-4 0.0 0.0 1.3912438023693214 1.1078325422485788E-4 70-71 2.2156650844971576E-4 0.0 0.0 1.556615505113478 1.1078325422485788E-4 72-73 2.2156650844971576E-4 0.0 0.0 1.7229011697049899 1.1078325422485788E-4 74-75 2.2156650844971576E-4 0.0 0.0 1.898437236024277 1.1078325422485788E-4 76-77 2.2156650844971576E-4 0.0 0.0 2.088098167257234 1.1078325422485788E-4 78-79 2.2156650844971576E-4 0.0 0.0 2.3031007678941267 1.1078325422485788E-4 80-81 2.2156650844971576E-4 0.0 0.0 2.536077951529003 1.1078325422485788E-4 82-83 2.2156650844971576E-4 0.0 0.0 2.780382722908371 1.1078325422485788E-4 84-85 2.2156650844971576E-4 0.0 0.0 3.0215855631694426 1.1078325422485788E-4 86-87 2.2156650844971576E-4 0.0 0.0 3.2722603716667393 1.1078325422485788E-4 88-89 2.2156650844971576E-4 0.0 0.0 3.5478614123646297 1.1078325422485788E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2050 0.0 57.762234 1 GTATCAA 4280 0.0 47.300777 1 CAACGCA 5255 0.0 37.558266 5 ATCAACG 5280 0.0 37.281044 3 TCAACGC 5435 0.0 36.74911 4 TATCAAC 5865 0.0 34.11082 2 AACGCAG 6085 0.0 33.115704 6 CGCAGAG 6350 0.0 31.513554 8 TAGCGCG 65 0.005744611 29.334522 4 ACGCAGA 6975 0.0 28.895805 7 GCAGAGT 7370 0.0 26.250648 9 AATATCG 185 2.5131703E-7 23.136675 5 GTACATG 5800 0.0 22.63867 1 CAGAGTA 7010 0.0 22.003193 10-11 ACATGGG 5415 0.0 21.300453 3 TACATGG 6130 0.0 21.108385 2 AGAGTAC 6935 0.0 20.699005 10-11 GAGTACT 4725 0.0 20.224028 12-13 GTACTTT 4835 0.0 20.051664 14-15 TATACGA 145 5.936831E-4 19.757383 2 >>END_MODULE