FastQCFastQC Report
Sat 18 Jun 2016
SRR1931140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805327
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60200.3334575952168222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51850.28720558657794404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33530.18572812570797423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.058.180711
GTATCAA52850.040.7432631
ATCAACG66400.031.541043
TCAACGC68250.030.686084
CAACGCA68700.030.4850775
TATCAAC71350.029.6190762
AACGCAG75900.027.7183536
GTGGTAT13150.027.472011
CGCAGAG82200.024.7273438
ACGCAGA87750.023.650477
CTAACGC1852.548295E-723.103423
TAACGCC1703.1561922E-622.3484064
GCAGAGT93000.021.4983239
TCTAACG1856.56703E-620.5363732
GTACTTT59150.020.2325814-15
CAGAGTA88300.020.16861310-11
GTACATG62150.020.0383851
AGAGTAC85750.019.63304110-11
TGGTATC18150.019.6241172
ACATGGG59750.019.4730363
Produced by FastQC (version 0.11.5)