##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931140_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1805327 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.022320056144952 31.0 31.0 34.0 28.0 34.0 2 31.016861765209295 31.0 31.0 34.0 28.0 34.0 3 31.163985804233803 31.0 31.0 34.0 28.0 34.0 4 34.8614428300247 35.0 35.0 37.0 32.0 37.0 5 34.70618397664246 35.0 35.0 37.0 32.0 37.0 6 34.849686511086354 35.0 35.0 37.0 32.0 37.0 7 34.673916692100654 35.0 35.0 37.0 30.0 37.0 8 34.74786174471439 35.0 35.0 37.0 32.0 37.0 9 36.08745174696883 38.0 35.0 39.0 30.0 39.0 10-11 35.95161873721492 37.0 35.0 39.0 30.0 39.0 12-13 35.884016856779965 37.0 35.0 39.0 30.0 39.0 14-15 36.74333375615609 38.0 36.0 40.0 30.5 41.0 16-17 36.77840385702977 38.0 36.0 40.0 31.0 41.0 18-19 36.845174586099915 38.5 36.0 40.0 31.0 41.0 20-21 36.82127559162412 39.0 36.0 40.0 31.0 41.0 22-23 36.67591023676043 38.5 35.0 40.0 30.0 41.0 24-25 36.52161852118758 38.0 35.0 40.0 30.0 41.0 26-27 36.220373095843584 38.0 34.5 40.0 29.5 41.0 28-29 35.913707876744766 38.0 34.0 40.0 28.5 41.0 30-31 35.6450515612961 38.0 34.0 40.0 27.5 41.0 32-33 35.46447762649093 38.0 34.0 40.0 27.0 41.0 34-35 35.22218717163151 38.0 33.5 40.0 27.0 41.0 36-37 35.48631826810323 38.0 34.0 40.0 27.5 41.0 38-39 35.457761391703556 38.0 34.0 40.0 27.0 41.0 40-41 35.2822917953368 38.0 34.0 40.0 27.0 41.0 42-43 35.09425882402468 38.0 33.5 40.0 26.5 41.0 44-45 34.79748793431882 38.0 33.0 40.0 25.5 41.0 46-47 34.519929630476916 38.0 33.0 40.0 24.5 41.0 48-49 34.21732018631528 37.5 33.0 40.0 24.0 41.0 50-51 33.816927348895796 37.0 32.5 40.0 23.0 41.0 52-53 33.652662924777616 37.0 32.0 40.0 23.0 41.0 54-55 33.35115161962348 37.0 32.0 40.0 22.0 41.0 56-57 33.103827450650215 36.5 31.0 39.5 22.0 41.0 58-59 32.822937894353764 36.0 31.0 39.0 20.5 40.5 60-61 32.499006274209606 36.0 31.0 39.0 19.5 40.0 62-63 32.202115738589185 35.5 30.5 39.0 19.0 40.0 64-65 31.936744146628286 35.0 30.0 39.0 18.0 40.0 66-67 31.658478214750012 35.0 30.0 38.0 18.0 40.0 68-69 31.31899234875455 35.0 30.0 38.0 16.0 40.0 70-71 30.50792875750487 34.0 29.0 37.0 9.5 40.0 72-73 30.432476498717406 34.0 29.0 37.0 10.5 39.0 74-75 30.13239318971023 34.0 29.0 36.0 8.0 39.0 76-77 29.47172783656368 33.5 28.5 36.0 7.0 39.0 78-79 29.510421380724935 34.0 29.0 35.5 7.0 38.0 80-81 29.2426064640921 34.0 29.0 35.0 4.0 37.0 82-83 28.895567949739853 33.5 28.0 35.0 2.0 37.0 84-85 28.535482491537543 33.0 27.5 35.0 2.0 36.0 86-87 28.19531558548673 33.0 27.0 35.0 2.0 36.0 88-89 27.868681961772023 33.0 26.5 35.0 2.0 36.0 90-91 27.542251902286957 33.0 26.0 35.0 2.0 35.0 92-93 27.157232457056256 33.0 25.5 35.0 2.0 35.0 94-95 26.724050822925708 32.5 25.0 35.0 2.0 35.0 96-97 26.294831905798784 32.0 25.0 34.5 2.0 35.0 98-99 25.75254178328912 32.0 23.5 34.0 2.0 35.0 100-101 25.014942168371714 31.5 20.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7319379156733028 1101 2 0.6256889904966059 1101 3 0.49549882969170866 1101 4 0.8153262181683232 1101 5 0.8384118823109219 1101 6 0.8778326922127988 1101 7 0.8205229695575014 1101 8 0.6752937747695427 1101 9 1.0876721102797617 1101 10-11 0.7669189011130868 1101 12-13 0.9698824285898482 1101 14-15 1.217785068702078 1101 16-17 1.0510616611860257 1101 18-19 0.902923573513732 1101 20-21 0.986026684782523 1101 22-23 0.7813853988975907 1101 24-25 0.6404195067495664 1101 26-27 1.078449997857767 1101 28-29 0.8712491106591074 1101 30-31 1.093250812554139 1101 32-33 1.3935779418774246 1101 34-35 0.9385446791415433 1101 36-37 1.2080745784538394 1101 38-39 1.0854709251491315 1101 40-41 1.0686538456270966 1101 42-43 1.0004049643190456 1101 44-45 0.9671317879179284 1101 46-47 0.9622249786671375 1101 48-49 0.9064556432454438 1101 50-51 0.7519629962430514 1101 52-53 0.6505399931320639 1101 54-55 0.8394731902778645 1101 56-57 0.49578502794516055 1101 58-59 0.8320494670356098 1101 60-61 0.7993426904347025 1101 62-63 0.772154499910922 1101 64-65 0.6648510763551485 1101 66-67 0.6320170758256154 1101 68-69 0.4684080837463256 1101 70-71 0.6686710260494095 1101 72-73 0.24384550631372903 1101 74-75 0.0475546308522361 1101 76-77 0.0595403992599941 1101 78-79 0.3364359364447864 1101 80-81 0.22128489477482205 1101 82-83 0.12419699623288949 1101 84-85 0.08907254263552744 1101 86-87 0.06918404154755464 1101 88-89 -0.1397580110046981 1101 90-91 0.016258305862173472 1101 92-93 -0.2887016379842535 1101 94-95 -0.2675476633700953 1101 96-97 -0.2796446083157278 1101 98-99 -0.3307976791637941 1101 100-101 -0.20957684293312084 1102 1 0.9598642782754325 1102 2 0.8036814091181981 1102 3 0.6264078306674854 1102 4 0.9753290061010702 1102 5 1.1214577438046263 1102 6 1.0336728267530333 1102 7 1.1811611363568204 1102 8 1.0529679193913069 1102 9 1.356577127209711 1102 10-11 1.2238726798861208 1102 12-13 1.3696005775823679 1102 14-15 1.658872857106978 1102 16-17 1.496289169988259 1102 18-19 1.3145870250644123 1102 20-21 1.3386355603013342 1102 22-23 0.988165606373741 1102 24-25 0.8462684997212762 1102 26-27 1.0538232435960495 1102 28-29 1.2725476577864185 1102 30-31 1.8008754488862948 1102 32-33 1.917044376516138 1102 34-35 1.7810601589132915 1102 36-37 1.7799108368682326 1102 38-39 1.6522022538688859 1102 40-41 1.6953682219170787 1102 42-43 1.6927176444667182 1102 44-45 1.7351883739593745 1102 46-47 1.5307612690078543 1102 48-49 1.324618800264517 1102 50-51 1.173489299489198 1102 52-53 1.299711437506069 1102 54-55 1.0918830433717943 1102 56-57 1.0677117143072223 1102 58-59 1.3699800565035218 1102 60-61 1.5417657637258522 1102 62-63 1.419952662573312 1102 64-65 1.3548012982656026 1102 66-67 1.0122557138755006 1102 68-69 0.8204658029475382 1102 70-71 1.146149475778934 1102 72-73 0.6932045515816618 1102 74-75 0.7739258216142879 1102 76-77 0.8266972474570551 1102 78-79 0.8832416400155907 1102 80-81 0.643583725192066 1102 82-83 0.7452804228720353 1102 84-85 0.690134834945777 1102 86-87 0.7012450433147457 1102 88-89 0.6191900839828683 1102 90-91 0.7089851289228726 1102 92-93 0.4796671515582993 1102 94-95 0.4567890109858652 1102 96-97 0.642353170331404 1102 98-99 0.5451710600161057 1102 100-101 0.24203210902900096 1103 1 0.6542801315732731 1103 2 0.41190382852074237 1103 3 0.2874607735837813 1103 4 0.6907918869055649 1103 5 0.7711590156176982 1103 6 0.789466970497493 1103 7 0.7587653954517606 1103 8 0.5177239087435694 1103 9 0.8568019127761133 1103 10-11 0.7705925852395694 1103 12-13 1.0219046296379801 1103 14-15 1.187127811262549 1103 16-17 1.0474485902781083 1103 18-19 0.8757227837156947 1103 20-21 0.8048615289496581 1103 22-23 0.5872516755840351 1103 24-25 0.40902508061450504 1103 26-27 0.9117676387454097 1103 28-29 0.8231125794072369 1103 30-31 1.3158828431483514 1103 32-33 1.3730662635318183 1103 34-35 1.2750353141927988 1103 36-37 1.3633624783944 1103 38-39 1.2034178705503464 1103 40-41 1.040180407660948 1103 42-43 1.3531967926477293 1103 44-45 1.258583819093957 1103 46-47 1.3177946910723293 1103 48-49 1.2424981259078152 1103 50-51 1.0338530971821882 1103 52-53 0.7773009080769029 1103 54-55 0.8665917094469222 1103 56-57 1.0943961272028204 1103 58-59 1.2386550047436842 1103 60-61 1.2078053969704605 1103 62-63 0.9598609737238597 1103 64-65 0.8278920998036092 1103 66-67 0.97093953523013 1103 68-69 0.6520895828943019 1103 70-71 0.8829137467046984 1103 72-73 0.36388482369050834 1103 74-75 0.3083446326169472 1103 76-77 0.36463572719677373 1103 78-79 0.41112096565888834 1103 80-81 0.22698804344110357 1103 82-83 0.2410094971104293 1103 84-85 0.4457633472202538 1103 86-87 0.4798756259825936 1103 88-89 0.1385719236752685 1103 90-91 0.262158746195869 1103 92-93 -0.060702561544328404 1103 94-95 -0.14429942438827936 1103 96-97 -0.11250042327995402 1103 98-99 -0.03476978588747315 1103 100-101 -0.24879309061607557 1104 1 0.501415579208178 1104 2 0.38589310150469913 1104 3 0.14608553231739663 1104 4 0.7480890325679539 1104 5 0.5403866887577777 1104 6 0.7743046168962522 1104 7 0.7131386821936232 1104 8 0.42676015434894055 1104 9 0.7961890885339074 1104 10-11 0.6588828184103761 1104 12-13 0.714981465930542 1104 14-15 1.0493091338139848 1104 16-17 0.8502169410070124 1104 18-19 0.7390859685234545 1104 20-21 0.7638732286541909 1104 22-23 0.42566128416076765 1104 24-25 0.5138013165234767 1104 26-27 0.2540561094900582 1104 28-29 0.6736951252897825 1104 30-31 1.199967037112799 1104 32-33 1.261286975393432 1104 34-35 1.0956410643368741 1104 36-37 1.2446191675335285 1104 38-39 0.7263108107833602 1104 40-41 0.962331787262535 1104 42-43 1.2688073757424618 1104 44-45 1.1617192911222958 1104 46-47 1.2077128815668132 1104 48-49 0.9268140575695938 1104 50-51 0.8920649969129499 1104 52-53 0.7326889619986048 1104 54-55 1.021724242065524 1104 56-57 0.8580816103606708 1104 58-59 1.0152360809213619 1104 60-61 1.0759951291609546 1104 62-63 0.18518990404094282 1104 64-65 -0.7870223554450995 1104 66-67 0.0619368306072694 1104 68-69 0.33035075737754127 1104 70-71 0.8873097498392539 1104 72-73 0.5364345786888762 1104 74-75 0.5082595604885931 1104 76-77 0.20917723721180792 1104 78-79 0.2906066909216527 1104 80-81 0.23099680068671802 1104 82-83 0.034842225181485276 1104 84-85 -0.06779605233517572 1104 86-87 0.3123195134434482 1104 88-89 0.3313420124369024 1104 90-91 0.4088758463035056 1104 92-93 0.41171363936005534 1104 94-95 0.5122994590019161 1104 96-97 0.6452760535142623 1104 98-99 0.702536324364786 1104 100-101 0.34600470299711006 1105 1 -0.4423665305859785 1105 2 -1.9653596499504147 1105 3 -0.4474041891340512 1105 4 0.429189409747778 1105 5 0.7010092972396578 1105 6 0.21940788040086545 1105 7 0.029847156561558563 1105 8 -0.05324089274049015 1105 9 0.5978586271089128 1105 10-11 1.0036170967039908 1105 12-13 1.1285033170936103 1105 14-15 1.1898399740122017 1105 16-17 0.8572423989379203 1105 18-19 0.7210919642486004 1105 20-21 1.1539213833866953 1105 22-23 0.35209511379931513 1105 24-25 0.09224020861180549 1105 26-27 1.2248748082310428 1105 28-29 0.7614654052496235 1105 30-31 1.2936535999962118 1105 32-33 1.3730969491479144 1105 34-35 0.9751754737442866 1105 36-37 1.0574560287236139 1105 38-39 0.9653500037960825 1105 40-41 1.1526163907229403 1105 42-43 1.5293295433086413 1105 44-45 1.4223912949100281 1105 46-47 1.3647863121277126 1105 48-49 1.1253557579062559 1105 50-51 0.8082482762604712 1105 52-53 1.0947507493802604 1105 54-55 0.2727455526117666 1105 56-57 0.5509321520018204 1105 58-59 -0.2598478000473392 1105 60-61 0.22308791949495443 1105 62-63 0.8266242572242071 1105 64-65 1.2137586936019353 1105 66-67 1.0763683407150957 1105 68-69 0.8724204206927624 1105 70-71 0.16966100054205668 1105 72-73 -0.5003233251291306 1105 74-75 -0.31840701892062384 1105 76-77 -0.044193821691525415 1105 78-79 -0.43641026509830994 1105 80-81 -0.4085555799621545 1105 82-83 -0.4422057451722239 1105 84-85 -0.2052470838320808 1105 86-87 -0.6197481695069733 1105 88-89 -1.0525817503472616 1105 90-91 -1.2566205651389062 1105 92-93 -0.9473616099496631 1105 94-95 -0.9820808006307367 1105 96-97 -1.0126398856908168 1105 98-99 -1.662635091004212 1105 100-101 -1.3025284043773588 1106 1 0.40805980963530786 1106 2 0.2713175468166149 1106 3 -0.2893565770231916 1106 4 0.49856120082371547 1106 5 0.29166273317792246 1106 6 0.5965071734449481 1106 7 0.523724070609326 1106 8 0.5514404354299884 1106 9 0.9347924326226718 1106 10-11 0.24324020605838825 1106 12-13 0.4375909716333908 1106 14-15 0.020801295745776827 1106 16-17 -0.2879706344409385 1106 18-19 0.39265853711376053 1106 20-21 0.6125131485572197 1106 22-23 0.48578192070721826 1106 24-25 0.4199575687628041 1106 26-27 0.8392294499858792 1106 28-29 0.8204716780831802 1106 30-31 0.5039472814626933 1106 32-33 0.616051778225291 1106 34-35 -0.9163996395984739 1106 36-37 -0.6394561468736484 1106 38-39 -0.5311108122928303 1106 40-41 0.6870685932353311 1106 42-43 0.9887607627510633 1106 44-45 0.7745742441409931 1106 46-47 0.2121626132489638 1106 48-49 0.1923439458577647 1106 50-51 0.8401721897555348 1106 52-53 0.3394026959938614 1106 54-55 1.026550706137897 1106 56-57 0.6178540776270935 1106 58-59 1.1204467438811676 1106 60-61 1.2974658687601028 1106 62-63 1.1080809818774178 1106 64-65 1.1085143328677312 1106 66-67 0.95184578444076 1106 68-69 0.9301497632810865 1106 70-71 1.2985896728291024 1106 72-73 0.49197900409681594 1106 74-75 0.5582755708635148 1106 76-77 0.6740571894663141 1106 78-79 0.728539804135302 1106 80-81 0.8254058474756008 1106 82-83 0.5996238602040691 1106 84-85 0.8360588651049099 1106 86-87 0.9337235556747565 1106 88-89 0.9122347950400673 1106 90-91 1.007645227250709 1106 92-93 0.468947297126892 1106 94-95 0.6645345223744386 1106 96-97 0.8363693742063312 1106 98-99 1.1206966997141201 1106 100-101 1.0122621488236625 1107 1 0.36415302562150487 1107 2 0.3790068037457246 1107 3 0.07985193663997592 1107 4 -1.0508650306385192 1107 5 0.2580053298841989 1107 6 0.5320684696958935 1107 7 0.5940144549204689 1107 8 0.41233466913745076 1107 9 0.8735696500565666 1107 10-11 0.8402097979512888 1107 12-13 0.9275688227591488 1107 14-15 1.008045754734603 1107 16-17 0.8745971179251129 1107 18-19 0.7905216692073083 1107 20-21 0.08480744251308892 1107 22-23 0.37869731504169835 1107 24-25 0.36175728399155815 1107 26-27 0.9613077815818301 1107 28-29 0.850617444549087 1107 30-31 1.3043435555972707 1107 32-33 1.4977080892457693 1107 34-35 1.4846685776338688 1107 36-37 1.379620058690442 1107 38-39 1.3766119280127072 1107 40-41 0.36507467442330466 1107 42-43 0.3328487254230552 1107 44-45 0.03667918906137402 1107 46-47 0.513174266330843 1107 48-49 0.9757357577093657 1107 50-51 -0.7016265502292498 1107 52-53 0.8139540295858225 1107 54-55 1.392281824872768 1107 56-57 1.1842131451204807 1107 58-59 1.464115568365429 1107 60-61 1.667473323958479 1107 62-63 1.5970310342155152 1107 64-65 1.5440757374791154 1107 66-67 1.4780020445318307 1107 68-69 1.3827901821592121 1107 70-71 1.5395723503289247 1107 72-73 1.190366925808565 1107 74-75 1.060735569606532 1107 76-77 1.2801921242224914 1107 78-79 1.360840643061838 1107 80-81 1.4562090367401126 1107 82-83 1.344581632140013 1107 84-85 1.3006480815072123 1107 86-87 1.3502480895623208 1107 88-89 1.413525909700585 1107 90-91 1.473084639677051 1107 92-93 1.3065045927103363 1107 94-95 1.4147332554241565 1107 96-97 1.2705881929474856 1107 98-99 1.3484951622961745 1107 100-101 1.3764325521195566 1108 1 0.49108165225431577 1108 2 0.34638262276720155 1108 3 0.17401856206209843 1108 4 0.6077645337048736 1108 5 0.7780867275793284 1108 6 0.6645986131126946 1108 7 0.5765348900059948 1108 8 0.3517211787448957 1108 9 0.7045160029601192 1108 10-11 0.5423010867227518 1108 12-13 0.9403177653124786 1108 14-15 0.9448193907140521 1108 16-17 0.570088645026587 1108 18-19 0.5527117164545174 1108 20-21 0.5307695381420601 1108 22-23 0.5577383603434427 1108 24-25 -1.546605015702717 1108 26-27 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2.378792839446696 2311 92-93 2.503960985033718 2311 94-95 2.3557508372622706 2311 96-97 2.441868184066365 2311 98-99 2.474302442037512 2311 100-101 2.7667493582475267 2312 1 -0.12262916004477731 2312 2 -0.09142300717759255 2312 3 -0.23563028413338216 2312 4 0.3201641082350406 2312 5 -1.4130087630993984 2312 6 0.07350735830615918 2312 7 0.3028363617362544 2312 8 -2.612977533079306 2312 9 -5.112899079568791 2312 10-11 -0.6664668157204119 2312 12-13 -5.818520499674275 2312 14-15 -2.103558387900442 2312 16-17 -2.5847977865334855 2312 18-19 -2.03021783628445 2312 20-21 -3.2173378506310044 2312 22-23 -2.0567984470326266 2312 24-25 -2.7246387238322995 2312 26-27 -3.2604435600100246 2312 28-29 -6.624624543514507 2312 30-31 -6.920949839257034 2312 32-33 -2.2694066105887316 2312 34-35 0.37921318869761933 2312 36-37 0.875477423653571 2312 38-39 1.0817275306005953 2312 40-41 -2.3400785428894935 2312 42-43 -5.597597886744055 2312 44-45 -3.8369416043585503 2312 46-47 0.1392663471774256 2312 48-49 -1.1622663988681978 2312 50-51 -6.268771341843678 2312 52-53 -1.247085892175555 2312 54-55 1.1299316568603288 2312 56-57 1.7216184813961206 2312 58-59 1.9420181713043903 2312 60-61 2.2896534734143223 2312 62-63 2.240906103175842 2312 64-65 1.8438701103162707 2312 66-67 1.933031133252424 2312 68-69 1.8832365435614804 2312 70-71 1.770935507959699 2312 72-73 1.8570879248556587 2312 74-75 1.7252849452021017 2312 76-77 1.646688688744284 2312 78-79 1.5825004554897433 2312 80-81 1.5835323729154496 2312 82-83 0.9113628147469655 2312 84-85 1.204150012396422 2312 86-87 1.6773833521131003 2312 88-89 1.8805377959536536 2312 90-91 2.0380465234224765 2312 92-93 2.239821208388154 2312 94-95 2.233770067701606 2312 96-97 2.5921928371121865 2312 98-99 2.4464388906677073 2312 100-101 2.6155155051262255 2313 1 -0.7460616476504924 2313 2 -0.3454329226048287 2313 3 -0.7394214769009508 2313 4 -0.6024809777958708 2313 5 -4.45303500981856 2313 6 -2.842430232828285 2313 7 -1.7977760617108132 2313 8 -1.8134733044412172 2313 9 -0.7148238389738637 2313 10-11 0.2550482032355603 2313 12-13 -4.7164863789393685 2313 14-15 -1.2127374488511506 2313 16-17 -4.033871860607562 2313 18-19 -2.504736646433173 2313 20-21 -1.2326848905843448 2313 22-23 -2.3404817366214274 2313 24-25 -1.8004625791837157 2313 26-27 -7.870315242716352 2313 28-29 -4.107709991166885 2313 30-31 -4.542377369149129 2313 32-33 -8.508543385158575 2313 34-35 -3.1910332388322757 2313 36-37 -3.044324267801656 2313 38-39 -2.0320811599797466 2313 40-41 -1.7455174463598908 2313 42-43 -1.781871727513952 2313 44-45 -6.098789081757211 2313 46-47 -2.685245172215982 2313 48-49 -2.4775551127789583 2313 50-51 0.08410121575329299 2313 52-53 -1.5490325238465914 2313 54-55 -6.921176537948657 2313 56-57 -7.579599074742696 2313 58-59 -2.75789433963174 2313 60-61 -9.638459679108283 2313 62-63 -2.805280831790615 2313 64-65 -2.515053774198165 2313 66-67 -4.261158134755739 2313 68-69 -6.151321636665987 2313 70-71 -6.195235387345058 2313 72-73 -2.14597419237969 2313 74-75 0.7017358055112268 2313 76-77 -1.2480692581790152 2313 78-79 0.43905484452444554 2313 80-81 0.5020217284126787 2313 82-83 0.6316894405854043 2313 84-85 0.7488787962984382 2313 86-87 1.2681532558751307 2313 88-89 -1.758861445807792 2313 90-91 -0.26608733484524194 2313 92-93 1.5584272159705392 2313 94-95 0.9962737130792654 2313 96-97 1.3242211252428397 2313 98-99 2.514607452930761 2313 100-101 2.2449628658728003 2314 1 -28.486802388391233 2314 2 -14.677840330012238 2314 3 -5.624606662086137 2314 4 -4.718221718536611 2314 5 -1.5947016764852293 2314 6 -0.876689492087543 2314 7 -0.8199982839330318 2314 8 -4.88384367481159 2314 9 -4.47037939452942 2314 10-11 -1.7815258708385144 2314 12-13 0.13768028772729934 2314 14-15 -1.105330041443743 2314 16-17 -1.6972977865334826 2314 18-19 -3.9783477575442845 2314 20-21 -2.1669441498435944 2314 22-23 -6.30020395884365 2314 24-25 -2.8416662828874166 2314 26-27 -3.2832782056793164 2314 28-29 -2.176691472648365 2314 30-31 -7.041914406186166 2314 32-33 -3.704839681454871 2314 34-35 -4.653070275869311 2314 36-37 -7.996176119653512 2314 38-39 -7.59504412294271 2314 40-41 -8.200610038952483 2314 42-43 -7.14159394973618 2314 44-45 -4.827492785460912 2314 46-47 -7.268115542586351 2314 48-49 -7.185888446112294 2314 50-51 -2.289046932394861 2314 52-53 -3.9268103016243714 2314 54-55 -6.088769130541248 2314 56-57 -4.608302778446397 2314 58-59 -2.306505450742854 2314 60-61 -1.5972559754045825 2314 62-63 -1.5728734243832072 2314 64-65 -0.39422044086483155 2314 66-67 0.7717122356146326 2314 68-69 1.450067252222901 2314 70-71 0.7793016496919805 2314 72-73 -1.407085303490799 2314 74-75 0.38414321291863374 2314 76-77 -0.5804766655864242 2314 78-79 -2.71001922954963 2314 80-81 -3.7419597530688016 2314 82-83 -2.3831253742294116 2314 84-85 -0.7441767592571189 2314 86-87 0.7903754780973529 2314 88-89 1.028175591229246 2314 90-91 0.8089126651547573 2314 92-93 1.1787975863409095 2314 94-95 0.716644083449637 2314 96-97 1.985795199316911 2314 98-99 1.9655333788566836 2314 100-101 2.0468147177246507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 27.0 4 65.0 5 125.0 6 275.0 7 681.0 8 1665.0 9 2752.0 10 4083.0 11 5804.0 12 7573.0 13 9518.0 14 11629.0 15 14013.0 16 16396.0 17 18163.0 18 16629.0 19 14027.0 20 13914.0 21 14473.0 22 16234.0 23 18198.0 24 21809.0 25 26887.0 26 33168.0 27 41314.0 28 51097.0 29 62635.0 30 75217.0 31 89486.0 32 105741.0 33 126050.0 34 150017.0 35 180434.0 36 215051.0 37 236703.0 38 173522.0 39 29935.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.834231720745485 22.12073395349972 15.470818950078788 22.574215375676008 2 15.892910259471 22.187116239883412 38.79629562954523 23.12367787110036 3 19.065054859103476 26.441605950607695 30.843130162342174 23.65020902794665 4 13.730451559083887 18.587255576979853 37.55765588761679 30.124636976319476 5 12.763913845935686 38.350733718711986 35.22885642687961 13.656496008472718 6 30.180072640579798 38.81784297249196 17.466087861091093 13.535996525837145 7 26.487999127027955 33.08458799984712 22.433387414025272 17.994025459099653 8 25.696840516981133 35.928948052070346 20.015709065449087 18.358502365499437 9 25.73832884568834 15.865491404050347 20.887185534808932 37.50899421545238 10-11 24.576240204683142 26.945894012552852 28.2153593227155 20.262506460048513 12-13 25.076149639372815 24.57864974046253 28.389565989984085 21.95563463018057 14-15 22.48598176396852 25.978146895271603 26.23361307951413 25.302258261245747 16-17 21.757554171626523 28.928388042720236 26.938914667536686 22.375143118116554 18-19 21.748248378271636 27.781421315916727 29.172028114574257 21.29830219123738 20-21 22.635151415782293 26.885184789237627 29.191581358944944 21.288082436035133 22-23 22.271145338212968 26.782294841876293 29.068528859314686 21.87803096059606 24-25 22.13348158218532 26.928994007444572 29.126966056220443 21.810558354149666 26-27 22.16030159898315 27.038600337861325 28.903623658269805 21.897474404885724 28-29 21.979537081941686 27.005972251726963 29.239688012649662 21.77480265368169 30-31 22.278396171284076 26.77600183902443 29.15867401907157 21.786927970619924 32-33 22.17740707033723 26.967923084976213 28.929905385037642 21.924764459648916 34-35 22.26245845243796 27.11098727891961 29.115240840841505 21.511313427800925 36-37 22.344019819228475 26.728622527616334 28.974017035784566 21.95334061737063 38-39 22.2931636868205 26.9346335627014 28.966957427274075 21.805245323204026 40-41 22.217680054705422 26.872875532700697 29.14096810761392 21.768476304979963 42-43 22.258217737323214 26.99422677728433 28.78105928735527 21.966496198037188 44-45 22.275401438303767 27.199013361058782 28.602669552949312 21.92291564768814 46-47 22.421998209883537 26.837812733650786 28.543077343724242 22.197111712741435 48-49 22.588236597621172 27.184766410539083 28.192693993097294 22.034302998742444 50-51 22.544371793091386 27.24021043113769 27.896012934768176 22.31940484100275 52-53 22.503189197662678 27.270091114394013 27.80343353202464 22.42328615591867 54-55 22.624646130164255 27.36549067457052 27.736967422087684 22.272895773177538 56-57 22.528743654488437 27.36123378153853 27.606122267542194 22.50390029643084 58-59 22.58920413457733 27.54048262585155 27.699790313195255 22.170522926375863 60-61 22.747848928383288 27.478960129355112 27.799633626790115 21.973557315471485 62-63 22.501751133285826 27.42846665534789 27.80201405947455 22.267768151891737 64-65 22.486538440231783 27.255476029560032 27.820307566172154 22.43767796403603 66-67 22.682702411953798 27.273867906185238 27.555246820742322 22.488182861118634 68-69 22.526190426732583 27.233363106984342 27.626098336137595 22.614348130145476 70-71 22.573656473734662 27.03069955681339 27.707735588125885 22.687908381326057 72-73 22.537279576256843 27.081234702556912 27.767762206172076 22.613723515014172 74-75 22.40692113628099 27.44234302077122 27.557087825058346 22.593648017889443 76-77 22.36096286157577 27.358838592676012 27.437910140379003 22.84228840536922 78-79 22.28011878180518 27.492692459593194 27.308736865952817 22.91845189264881 80-81 22.34493023276425 27.391086754763478 27.419807353193292 22.844175659278985 82-83 22.405239843772936 27.20765448360707 27.64115079811291 22.74595487450708 84-85 22.456602725324203 27.279580729579937 27.255042231972997 23.008774313122863 86-87 22.219037157792524 27.602959284905953 27.422103017723913 22.75590053957761 88-89 22.407750899246455 27.073446146167747 27.517370752104338 23.00143220248147 90-91 22.41319047273505 27.21412970565052 27.48444443952068 22.888235382093754 92-93 22.36481392544388 27.330744248370465 27.39882266983738 22.905619156348273 94-95 22.21077146903131 27.02571932717578 27.4832847776469 23.280224426146013 96-97 22.359467971847618 27.00701483062818 27.35332500977667 23.280192187747534 98-99 21.971167601093562 27.215655276379962 27.501951896517774 23.311225226008705 100-101 22.23107280617766 27.135327244340836 27.372271163551126 23.261328785930377 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 519.0 1 354.5 2 200.0 3 237.0 4 204.0 5 179.5 6 324.5 7 659.0 8 1073.0 9 1325.0 10 1242.5 11 1059.0 12 1082.0 13 1209.0 14 1351.0 15 1498.0 16 1655.5 17 1906.5 18 2190.0 19 2429.5 20 2757.5 21 3204.0 22 3750.0 23 4588.5 24 5746.5 25 7205.5 26 9495.5 27 12332.0 28 15084.0 29 18594.0 30 23149.5 31 27590.5 32 32754.5 33 38321.5 34 43405.5 35 47959.0 36 53197.0 37 58051.0 38 59521.0 39 59470.5 40 58616.0 41 56907.0 42 55692.5 43 57000.5 44 59377.0 45 61338.0 46 61971.0 47 61377.5 48 60483.5 49 58819.5 50 57177.0 51 56607.0 52 56461.0 53 56383.5 54 56006.5 55 53695.5 56 50099.0 57 45308.0 58 41262.5 59 38065.0 60 34102.5 61 31533.5 62 26766.0 63 18741.5 64 12407.5 65 8671.5 66 6421.5 67 4554.0 68 3233.0 69 2384.0 70 1834.5 71 1363.5 72 1022.5 73 787.5 74 574.0 75 432.0 76 330.0 77 239.0 78 175.0 79 118.5 80 78.0 81 60.5 82 49.5 83 44.0 84 40.5 85 30.5 86 23.5 87 17.0 88 9.5 89 7.0 90 4.5 91 3.0 92 1.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09555055676893992 2 0.0 3 0.00365584738942031 4 2.2156650844971576E-4 5 1.6617488133728681E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.014069473286556953 26-27 0.008225656626195697 28-29 0.00340658506741438 30-31 0.0012186157964734366 32-33 5.539162711242894E-4 34-35 9.139618473550775E-4 36-37 5.262204575680749E-4 38-39 2.492623220059302E-4 40-41 0.0011909199829172224 42-43 5.262204575680749E-4 44-45 3.3234976267457363E-4 46-47 0.02307061269232665 48-49 0.013127815625645658 50-51 0.08344748624487419 52-53 7.754827795740051E-4 54-55 0.035339858097729666 56-57 8.585702202426486E-4 58-59 8.585702202426486E-4 60-61 0.003545064135195452 62-63 0.003738934830088953 64-65 0.01035823427002421 66-67 0.006093078982367183 68-69 0.002187969270940943 70-71 0.005843816660361253 72-73 0.004569809236775388 74-75 9.139618473550775E-4 76-77 0.0 78-79 0.0 80-81 1.3847906778107234E-4 82-83 3.3234976267457363E-4 84-85 2.769581355621447E-5 86-87 0.0014401823049231524 88-89 0.0036281515758640956 90-91 0.0013293990506982945 92-93 0.0031019311182960206 94-95 6.370037117929328E-4 96-97 4.985246440118604E-4 98-99 0.0017448362540415114 100-101 0.0026311022878403745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1805327.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.42301225943477 #Duplication Level Percentage of deduplicated Percentage of total 1 82.34525599941279 48.932031567593995 2 10.774767915629242 12.805383318860017 3 2.8724979945662463 5.120774506389355 4 1.1846517807424657 2.8158230916088307 5 0.6224821862123137 1.8494883291287036 6 0.3985666610351851 1.4210418950937473 7 0.26050992142111296 1.0836198978017828 8 0.19278314442827743 0.916460412381913 9 0.15347985619856042 0.8208211838817007 >10 1.0193005140885698 11.962164228421756 >50 0.11621016795278795 4.761759290432728 >100 0.057507858586839204 5.9473952157743 >500 0.0015131426481256288 0.5982752190664417 >1k 2.837142465235554E-4 0.33527239873665143 >5k 1.891428310157036E-4 0.6296894448280554 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6020 0.3334575952168222 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5185 0.28720558657794404 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3353 0.18572812570797423 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.1078325422485788E-4 0.0 0.0 0.0 0.0 7 1.1078325422485788E-4 0.0 0.0 0.0 0.0 8 1.1078325422485788E-4 0.0 0.0 0.0 0.0 9 1.1078325422485788E-4 0.0 0.0 0.0 0.0 10-11 1.1078325422485788E-4 0.0 0.0 8.308744066864341E-5 0.0 12-13 1.1078325422485788E-4 0.0 0.0 1.1078325422485788E-4 0.0 14-15 1.1078325422485788E-4 0.0 0.0 1.1078325422485788E-4 0.0 16-17 2.2156650844971576E-4 0.0 0.0 1.3847906778107234E-4 0.0 18-19 2.2156650844971576E-4 0.0 0.0 1.6617488133728681E-4 0.0 20-21 2.2156650844971576E-4 0.0 0.0 1.6617488133728681E-4 2.769581355621447E-5 22-23 2.2156650844971576E-4 0.0 0.0 1.938706948935013E-4 8.308744066864341E-5 24-25 2.2156650844971576E-4 0.0 0.0 3.0465394911835915E-4 1.1078325422485788E-4 26-27 2.2156650844971576E-4 0.0 0.0 7.200911524615762E-4 1.1078325422485788E-4 28-29 2.2156650844971576E-4 0.0 0.0 0.0024372315929468732 1.1078325422485788E-4 30-31 2.2156650844971576E-4 0.0 0.0 0.006702386880603902 1.1078325422485788E-4 32-33 2.2156650844971576E-4 0.0 0.0 0.018168453692876695 1.1078325422485788E-4 34-35 2.2156650844971576E-4 0.0 0.0 0.03486902926727402 1.1078325422485788E-4 36-37 2.2156650844971576E-4 0.0 0.0 0.059269041010298965 1.1078325422485788E-4 38-39 2.2156650844971576E-4 0.0 0.0 0.09599368978583936 1.1078325422485788E-4 40-41 2.2156650844971576E-4 0.0 0.0 0.13659575245924976 1.1078325422485788E-4 42-43 2.2156650844971576E-4 0.0 0.0 0.1770593360648791 1.1078325422485788E-4 44-45 2.2156650844971576E-4 0.0 0.0 0.2289058990421126 1.3847906778107234E-4 46-47 2.2156650844971576E-4 0.0 0.0 0.29462806461100954 1.6617488133728681E-4 48-49 2.2156650844971576E-4 0.0 0.0 0.3596301390274449 2.2156650844971576E-4 50-51 2.492623220059302E-4 0.0 0.0 0.43175003752782737 2.2156650844971576E-4 52-53 2.769581355621447E-4 0.0 0.0 0.5038976318417661 2.2156650844971576E-4 54-55 2.769581355621447E-4 0.0 0.0 0.5822213925787405 2.2156650844971576E-4 56-57 2.769581355621447E-4 0.0 0.0 0.6718727410602068 2.2156650844971576E-4 58-59 2.769581355621447E-4 0.0 0.0 0.774679600980875 2.2156650844971576E-4 60-61 3.0465394911835915E-4 0.0 0.0 0.8801452590029397 2.2156650844971576E-4 62-63 3.3234976267457363E-4 0.0 0.0 0.9873003616519334 2.2156650844971576E-4 64-65 3.3234976267457363E-4 0.0 0.0 1.0971419582158801 2.2156650844971576E-4 66-67 3.600455762307881E-4 0.0 0.0 1.2256782289302715 2.2156650844971576E-4 68-69 3.877413897870026E-4 0.0 0.0 1.3683947561854444 2.2156650844971576E-4 70-71 3.877413897870026E-4 0.0 0.0 1.5268148097269914 2.2156650844971576E-4 72-73 4.431330168994315E-4 0.0 0.0 1.6919095543355858 2.2156650844971576E-4 74-75 4.431330168994315E-4 0.0 0.0 1.8648976058077014 2.769581355621447E-4 76-77 4.431330168994315E-4 0.0 0.0 2.052259784515492 2.769581355621447E-4 78-79 4.431330168994315E-4 0.0 0.0 2.263689625203633 2.769581355621447E-4 80-81 4.431330168994315E-4 0.0 0.0 2.4917369540255034 2.769581355621447E-4 82-83 4.431330168994315E-4 0.0 0.0 2.729920950608948 2.769581355621447E-4 84-85 4.431330168994315E-4 0.0 0.0 2.9654461490909956 2.769581355621447E-4 86-87 4.431330168994315E-4 0.0 0.0 3.210664882317719 2.769581355621447E-4 88-89 4.431330168994315E-4 0.0 0.0 3.4799512775247923 2.769581355621447E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2835 0.0 58.18071 1 GTATCAA 5285 0.0 40.743263 1 ATCAACG 6640 0.0 31.54104 3 TCAACGC 6825 0.0 30.68608 4 CAACGCA 6870 0.0 30.485077 5 TATCAAC 7135 0.0 29.619076 2 AACGCAG 7590 0.0 27.718353 6 GTGGTAT 1315 0.0 27.47201 1 CGCAGAG 8220 0.0 24.727343 8 ACGCAGA 8775 0.0 23.65047 7 CTAACGC 185 2.548295E-7 23.10342 3 TAACGCC 170 3.1561922E-6 22.348406 4 GCAGAGT 9300 0.0 21.498323 9 TCTAACG 185 6.56703E-6 20.536373 2 GTACTTT 5915 0.0 20.23258 14-15 CAGAGTA 8830 0.0 20.168613 10-11 GTACATG 6215 0.0 20.038385 1 AGAGTAC 8575 0.0 19.633041 10-11 TGGTATC 1815 0.0 19.624117 2 ACATGGG 5975 0.0 19.473036 3 >>END_MODULE