FastQCFastQC Report
Sat 18 Jun 2016
SRR1931139_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931139_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561567
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43360.27766980219228504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35390.2266313260974393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19190.12288937970641028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.057.2889561
GTATCAA40900.041.1191561
ATCAACG47050.034.848863
CAACGCA47700.034.6833045
TCAACGC47900.034.238174
AACGCAG49700.033.463876
TATCAAC49400.033.0789762
ACGCAGA56200.029.0936437
CGCAGAG55750.028.4759068
GTACTAG5400.028.3126891
GAGTACT38150.023.67276412-13
GCAGAGT64250.023.598249
GTGGTAT9600.022.8934631
TATACCG1903.2619027E-722.5189675
GTACATG41700.022.4566461
TACATGG41950.021.640862
CAGAGTA61650.021.5481910-11
ACATGGG43800.020.8937743
GTACTTT43950.020.81274814-15
AGAGTAC56800.020.37570610-11
Produced by FastQC (version 0.11.5)