##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931139_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1561567 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.289969626663474 31.0 29.0 33.0 25.0 34.0 2 29.038405012401004 31.0 28.0 34.0 23.0 34.0 3 29.046187579527487 31.0 28.0 34.0 22.0 34.0 4 32.296258822067834 35.0 32.0 37.0 22.0 37.0 5 32.37198467949182 35.0 32.0 37.0 23.0 37.0 6 32.67323464186935 35.0 32.0 37.0 25.0 37.0 7 32.40974866912531 35.0 32.0 37.0 23.0 37.0 8 32.55002507097038 35.0 32.0 37.0 25.0 37.0 9 33.60116921015877 37.0 33.0 39.0 23.0 39.0 10-11 33.6320628573734 37.0 32.5 39.0 24.0 39.0 12-13 33.69542165017575 37.0 33.0 39.0 24.5 39.0 14-15 34.47644513491896 38.0 33.0 40.0 24.0 41.0 16-17 34.464877267513984 38.0 33.0 40.0 24.0 41.0 18-19 34.497701347428574 38.0 33.0 40.0 24.0 41.0 20-21 34.734414213415114 38.0 33.0 40.0 25.0 41.0 22-23 34.64842206578392 38.0 33.0 40.0 25.0 41.0 24-25 34.5122777312789 38.0 33.0 40.0 24.0 41.0 26-27 34.175532333867196 38.0 32.5 40.0 22.5 41.0 28-29 34.118295596666684 38.0 32.0 40.0 23.5 41.0 30-31 33.839139787149705 38.0 32.0 40.0 21.0 41.0 32-33 33.65135469691663 38.0 32.0 40.0 21.0 41.0 34-35 33.63928316876573 37.5 32.0 40.0 22.0 41.0 36-37 33.27430107065531 37.0 31.5 40.0 18.5 41.0 38-39 33.10963954796688 37.0 31.0 40.0 17.5 41.0 40-41 32.98772066776513 37.0 31.0 40.0 16.0 41.0 42-43 33.195393153159614 38.0 31.0 40.0 16.5 41.0 44-45 33.23835320546605 38.0 32.0 40.0 17.5 41.0 46-47 32.99947392587062 37.5 31.0 40.0 15.0 41.0 48-49 32.7821739316981 37.0 31.0 40.0 14.0 41.0 50-51 32.26025204169914 36.5 30.5 39.5 9.0 40.5 52-53 32.19366956397003 37.0 30.0 39.5 9.0 40.5 54-55 32.21627922465062 37.0 30.0 40.0 8.5 41.0 56-57 31.96901733963384 36.5 30.0 40.0 8.0 41.0 58-59 31.829372675011705 36.0 30.0 40.0 7.0 41.0 60-61 31.668844500428097 36.0 30.0 40.0 7.0 41.0 62-63 31.52324299885948 36.0 30.0 39.0 4.0 41.0 64-65 31.186118815266973 35.0 30.0 39.0 2.0 41.0 66-67 30.975198630606307 35.0 29.5 39.0 2.0 40.0 68-69 30.763770942905428 35.0 29.0 39.0 2.0 40.0 70-71 30.46305762096663 35.0 29.0 38.0 2.0 40.0 72-73 30.12847479486951 34.5 29.0 37.5 2.0 40.0 74-75 29.70594601448417 34.0 29.0 37.0 2.0 39.5 76-77 29.403013447389704 34.0 29.0 36.5 2.0 39.0 78-79 28.98327833515949 34.0 28.5 36.0 2.0 39.0 80-81 28.582817772148104 34.0 28.0 36.0 2.0 37.5 82-83 28.244388489254703 33.5 27.5 35.0 2.0 37.0 84-85 27.926653163136773 33.0 27.0 35.0 2.0 37.0 86-87 27.612013125277365 33.0 27.0 35.0 2.0 36.0 88-89 27.28282872268689 33.0 26.0 35.0 2.0 36.0 90-91 26.90344570549967 33.0 26.0 35.0 2.0 36.0 92-93 26.552611895615108 33.0 25.0 35.0 2.0 35.0 94-95 26.26758858249438 33.0 25.0 35.0 2.0 35.0 96-97 25.881076188213505 32.0 24.5 35.0 2.0 35.0 98-99 25.52995548702041 32.0 23.5 35.0 2.0 35.0 100-101 24.938791291055715 32.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.525820635361086 1101 2 6.260991102009626 1101 3 5.710443613531957 1101 4 7.205295364698209 1101 5 6.660599739885814 1101 6 6.162992412404005 1101 7 6.677972114283012 1101 8 6.253117362015441 1101 9 6.8922446324741955 1101 10-11 6.890029285427335 1101 12-13 6.7987877260047185 1101 14-15 7.200668328325378 1101 16-17 7.198994944398983 1101 18-19 7.252512461192314 1101 20-21 6.609712636074956 1101 22-23 6.60786915040584 1101 24-25 6.5417022235126225 1101 26-27 6.605920820543638 1101 28-29 6.484275591829341 1101 30-31 6.701699776907546 1101 32-33 6.564092473108616 1101 34-35 6.437935526329753 1101 36-37 6.643269968258508 1101 38-39 6.483755474647651 1101 40-41 6.773089694097141 1101 42-43 6.509804586724485 1101 44-45 6.540601984327957 1101 46-47 6.262960762951963 1101 48-49 6.368370919254847 1101 50-51 6.342198937367019 1101 52-53 6.096866758435581 1101 54-55 6.299829120642329 1101 56-57 6.232944063201771 1101 58-59 6.151861356953596 1101 60-61 5.936556783057515 1101 62-63 5.964147164588098 1101 64-65 5.973299279469053 1101 66-67 6.03207556277539 1101 68-69 5.9896761908016956 1101 70-71 5.834203019479638 1101 72-73 5.768032843495419 1101 74-75 5.742248463975866 1101 76-77 5.570576045916834 1101 78-79 5.63620026323667 1101 80-81 5.591929329348037 1101 82-83 5.445428994721851 1101 84-85 5.439827664620985 1101 86-87 5.355561624600984 1101 88-89 5.3942185366375135 1101 90-91 5.392126742354723 1101 92-93 5.329406364411749 1101 94-95 5.43628198260798 1101 96-97 5.39148626602983 1101 98-99 5.058611122451637 1101 100-101 5.046634854254506 1102 1 5.514660204773829 1102 2 6.179677445976825 1102 3 5.808744466926431 1102 4 7.180947837958634 1102 5 6.746045078231422 1102 6 6.064900311896924 1102 7 6.63233179302939 1102 8 6.232627770398604 1102 9 7.0240197549409515 1102 10-11 6.944502704203391 1102 12-13 7.064131028803125 1102 14-15 7.268883429173265 1102 16-17 7.211817342647443 1102 18-19 7.245455897559513 1102 20-21 6.610774163785319 1102 22-23 6.644450890724684 1102 24-25 6.352554068861984 1102 26-27 6.629256047249136 1102 28-29 6.523237679633613 1102 30-31 6.751980168112659 1102 32-33 6.455129937780008 1102 34-35 6.507720542526684 1102 36-37 6.652314848063796 1102 38-39 6.463230356484019 1102 40-41 6.742903007601715 1102 42-43 6.702688605630662 1102 44-45 6.555449602293649 1102 46-47 6.548586556877339 1102 48-49 6.273000661721806 1102 50-51 6.333379196724589 1102 52-53 5.928031113466957 1102 54-55 6.351940643705319 1102 56-57 6.145900787599075 1102 58-59 6.1977631012657355 1102 60-61 6.115158579351327 1102 62-63 5.900712931730865 1102 64-65 6.0285525610272686 1102 66-67 6.0014557415785745 1102 68-69 6.055802316927817 1102 70-71 5.868208465506477 1102 72-73 5.617130462321732 1102 74-75 5.71290319207041 1102 76-77 5.528758959437919 1102 78-79 5.577216212315285 1102 80-81 5.689688678303412 1102 82-83 5.602180794286031 1102 84-85 5.546519805749508 1102 86-87 5.541714257937414 1102 88-89 5.48631890601721 1102 90-91 5.508846320167155 1102 92-93 5.564582878334921 1102 94-95 5.462465460698223 1102 96-97 5.40104579881443 1102 98-99 5.169730105400959 1102 100-101 5.321338162836302 1103 1 5.257796443969919 1103 2 5.975573035189122 1103 3 5.436465640183762 1103 4 7.068293309543506 1103 5 6.47472864601723 1103 6 5.754174809051722 1103 7 6.366026256998172 1103 8 5.86219336734575 1103 9 6.526225758593341 1103 10-11 6.584692063494995 1103 12-13 6.575427177106754 1103 14-15 6.848306493134977 1103 16-17 6.768016805380569 1103 18-19 6.760330679716095 1103 20-21 6.2308422047511165 1103 22-23 6.380315797312782 1103 24-25 6.034328333329512 1103 26-27 6.125506797464979 1103 28-29 6.095851896985987 1103 30-31 6.395659579622496 1103 32-33 6.191388390746695 1103 34-35 6.141053747480839 1103 36-37 6.344924315386486 1103 38-39 6.0945795253893 1103 40-41 6.31718846512906 1103 42-43 6.262347052820282 1103 44-45 6.032097942832259 1103 46-47 6.017866445957722 1103 48-49 5.822698553414714 1103 50-51 5.7314378467533125 1103 52-53 5.49985207586149 1103 54-55 5.964214271091166 1103 56-57 5.8412930662332485 1103 58-59 5.964990592383501 1103 60-61 5.7528677170604645 1103 62-63 5.8086978600150445 1103 64-65 5.934545108615822 1103 66-67 5.980007945367689 1103 68-69 5.89330996351277 1103 70-71 5.677019963145909 1103 72-73 5.447107578732762 1103 74-75 5.469372122703927 1103 76-77 5.526506610901283 1103 78-79 5.413600476879104 1103 80-81 5.404641258243522 1103 82-83 5.447677862426492 1103 84-85 5.5641020406384705 1103 86-87 5.463039259461919 1103 88-89 5.338155979998923 1103 90-91 5.280386196345436 1103 92-93 5.088847763098556 1103 94-95 5.041325068435633 1103 96-97 4.997138193310814 1103 98-99 4.933353479398399 1103 100-101 4.933230118917784 1104 1 4.571684679673659 1104 2 5.50818827975678 1104 3 5.007904051440121 1104 4 6.599847871136284 1104 5 6.151269537818489 1104 6 5.662820838655978 1104 7 6.268536397066857 1104 8 5.6342117481440575 1104 9 6.577964960770011 1104 10-11 6.452653432835707 1104 12-13 6.3365748820047685 1104 14-15 6.552347559025499 1104 16-17 6.513863893447528 1104 18-19 6.595224866244916 1104 20-21 5.898569827721214 1104 22-23 6.1263644756977556 1104 24-25 5.925486718225223 1104 26-27 6.057237378438693 1104 28-29 5.819654301638057 1104 30-31 6.146080926094442 1104 32-33 5.90725011744977 1104 34-35 5.745947005533608 1104 36-37 5.828141290653704 1104 38-39 5.7959660454307596 1104 40-41 5.944771419923374 1104 42-43 5.908852311829783 1104 44-45 5.958558476039919 1104 46-47 5.889267592020133 1104 48-49 5.739024000925031 1104 50-51 5.599736545574853 1104 52-53 5.436645563611158 1104 54-55 5.6716716494986485 1104 56-57 5.529580189380468 1104 58-59 5.539341655740884 1104 60-61 5.333022512689762 1104 62-63 5.327096950310349 1104 64-65 5.454078554442781 1104 66-67 5.472596032010642 1104 68-69 5.518181856524073 1104 70-71 5.25793557930583 1104 72-73 5.145525939952336 1104 74-75 5.293031181249393 1104 76-77 5.343535265489013 1104 78-79 5.368417753800113 1104 80-81 5.327903784344297 1104 82-83 5.166143737399118 1104 84-85 5.136748795362347 1104 86-87 5.140176154678347 1104 88-89 5.17007008637604 1104 90-91 5.059349918666506 1104 92-93 5.174610377505477 1104 94-95 5.101160430455515 1104 96-97 5.037524820328862 1104 98-99 4.727383807261461 1104 100-101 4.682925841166188 1105 1 4.479796340465274 1105 2 5.270562698177049 1105 3 4.855348939012909 1105 4 6.272406368901834 1105 5 3.669893618019543 1105 6 4.855773797554949 1105 7 5.662211446116512 1105 8 1.1480002374903115 1105 9 0.743828448574611 1105 10-11 2.3171420516805874 1105 12-13 5.445667803249712 1105 14-15 3.7359419136906595 1105 16-17 5.83454069683237 1105 18-19 6.308332937193551 1105 20-21 3.3376699978690674 1105 22-23 5.405916025339529 1105 24-25 5.63906632967899 1105 26-27 5.77542235571665 1105 28-29 3.2934241433074156 1105 30-31 5.544885423434614 1105 32-33 5.689206484339898 1105 34-35 5.657660228992363 1105 36-37 5.835079425168672 1105 38-39 5.8646729185485285 1105 40-41 5.942201897784646 1105 42-43 3.2106614385257544 1105 44-45 5.362802010232464 1105 46-47 5.622792855579419 1105 48-49 5.637018895859985 1105 50-51 5.619644600288968 1105 52-53 5.56098375513573 1105 54-55 5.761873926610612 1105 56-57 5.6738015804399 1105 58-59 5.508647633065415 1105 60-61 5.444179514991543 1105 62-63 5.161763916078158 1105 64-65 5.236245718422779 1105 66-67 5.4644010591751595 1105 68-69 5.430813463125105 1105 70-71 5.269183727752285 1105 72-73 5.074805089414397 1105 74-75 5.20887799406032 1105 76-77 5.241649166392985 1105 78-79 5.241020487492285 1105 80-81 5.201643485176032 1105 82-83 5.079321764665565 1105 84-85 5.05608296286486 1105 86-87 5.010457839368691 1105 88-89 5.052846375290127 1105 90-91 5.051031535146688 1105 92-93 5.009989884630418 1105 94-95 4.999836719117901 1105 96-97 4.896364155820926 1105 98-99 4.856186335064656 1105 100-101 4.971128566300628 1106 1 4.375994937564872 1106 2 4.663838646011811 1106 3 4.655945114175623 1106 4 6.249478407362986 1106 5 6.061494654020802 1106 6 5.176469453854413 1106 7 5.916052601216219 1106 8 5.596695819029765 1106 9 6.334588721966227 1106 10-11 5.978439116343683 1106 12-13 6.2324523355467605 1106 14-15 6.152644253103993 1106 16-17 6.1576946276994775 1106 18-19 6.40967876119986 1106 20-21 5.782252340991658 1106 22-23 5.70457356193959 1106 24-25 5.7905871747834325 1106 26-27 5.970431154395122 1106 28-29 6.013315087138206 1106 30-31 6.216604501183596 1106 32-33 5.824399814452796 1106 34-35 5.4313945563463335 1106 36-37 5.625547595374215 1106 38-39 5.650923437914969 1106 40-41 5.7477024915516814 1106 42-43 2.620622437520808 1106 44-45 4.8630582789314545 1106 46-47 5.085248412908129 1106 48-49 4.869290349467807 1106 50-51 5.183838831116265 1106 52-53 5.2017816601301305 1106 54-55 5.2519194331744 1106 56-57 5.1630461125356675 1106 58-59 5.260641173512738 1106 60-61 5.094811776237538 1106 62-63 5.108341506907976 1106 64-65 5.235410778468008 1106 66-67 5.373722644403728 1106 68-69 5.286601757144737 1106 70-71 5.095521069372481 1106 72-73 4.857618085867607 1106 74-75 4.797567540885243 1106 76-77 4.946224191660431 1106 78-79 4.965491380687542 1106 80-81 4.862920332287494 1106 82-83 4.866023180361427 1106 84-85 5.122134692480895 1106 86-87 5.224685262922986 1106 88-89 5.282330232878053 1106 90-91 5.414503846625653 1106 92-93 5.440995018630556 1106 94-95 5.360817599244534 1106 96-97 5.331212421748042 1106 98-99 5.127624577543006 1106 100-101 5.181263597980184 1107 1 3.056575297123704 1107 2 -0.32276854516481635 1107 3 1.0147422372720278 1107 4 2.7100562775018524 1107 5 2.5942562325295313 1107 6 3.8224874642666826 1107 7 4.890828282830231 1107 8 4.184829403469802 1107 9 4.796802303375678 1107 10-11 2.429255483738782 1107 12-13 4.92020804227144 1107 14-15 3.371366179482102 1107 16-17 1.775813546208859 1107 18-19 2.171281622950456 1107 20-21 2.1350843267911337 1107 22-23 4.273328229602022 1107 24-25 4.153648106042972 1107 26-27 3.233190784662135 1107 28-29 4.097123304388806 1107 30-31 3.4956874205155657 1107 32-33 3.9974828257850774 1107 34-35 4.491342224409237 1107 36-37 4.7299462922169795 1107 38-39 4.704723321020467 1107 40-41 3.5361998830724986 1107 42-43 4.109522195072948 1107 44-45 4.356848258474912 1107 46-47 4.611242557359425 1107 48-49 2.2700490813789216 1107 50-51 4.077230774054424 1107 52-53 4.283120751600073 1107 54-55 4.755452650695588 1107 56-57 3.507230746320346 1107 58-59 2.5246509255448615 1107 60-61 3.7473333963087505 1107 62-63 4.4835126545305855 1107 64-65 4.538746601221305 1107 66-67 4.7406922355976775 1107 68-69 4.637669501838484 1107 70-71 4.460390212470834 1107 72-73 4.384635057217633 1107 74-75 4.409504174323569 1107 76-77 4.3421772606823055 1107 78-79 4.178347611272773 1107 80-81 4.354599040170296 1107 82-83 4.31662172350957 1107 84-85 4.1113693040772645 1107 86-87 4.221510575994603 1107 88-89 4.315739195872283 1107 90-91 4.344968529332842 1107 92-93 4.28523309376774 1107 94-95 4.207377329088352 1107 96-97 4.164476754854082 1107 98-99 4.280734052491866 1107 100-101 4.405800484689927 1108 1 -1.8543078622157836 1108 2 -0.8646548196874662 1108 3 -1.9432568389828084 1108 4 -3.9194411112256162 1108 5 -8.868962113093108 1108 6 -7.078213752216161 1108 7 -5.752479423672128 1108 8 -5.89936188821201 1108 9 -7.902774149639438 1108 10-11 -3.190543399686984 1108 12-13 -1.96847602487151 1108 14-15 -6.604454845438273 1108 16-17 -6.077109517611447 1108 18-19 -4.259675796328299 1108 20-21 -4.689983616521918 1108 22-23 -3.434016254187121 1108 24-25 -8.249416795244045 1108 26-27 -9.373916429831269 1108 28-29 -6.104348682438545 1108 30-31 -6.066164470159304 1108 32-33 -2.552592132968506 1108 34-35 -0.9477490112582423 1108 36-37 -4.796218298206856 1108 38-39 -5.8225110558391755 1108 40-41 -3.269854578583889 1108 42-43 -4.349008635194881 1108 44-45 -1.6040943000808134 1108 46-47 -2.215315552871978 1108 48-49 -1.059347416032736 1108 50-51 -0.36155855199376674 1108 52-53 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-5.992392469896089 2313 56-57 -4.438999234374116 2313 58-59 -3.5713601683453433 2313 60-61 -2.900442387469152 2313 62-63 -4.738188408633896 2313 64-65 -5.614005118603586 2313 66-67 -7.753393742051401 2313 68-69 -7.168860394564156 2313 70-71 -4.585915902764231 2313 72-73 -3.7700064306688184 2313 74-75 -5.642478042823019 2313 76-77 -4.894805092418817 2313 78-79 -6.708259507306774 2313 80-81 -6.99990921227009 2313 82-83 -7.158702528060704 2313 84-85 -6.4633988934374855 2313 86-87 -4.872515581658281 2313 88-89 -7.6627867850397 2313 90-91 -6.12056699558762 2313 92-93 -5.086276273499845 2313 94-95 -5.450098810890438 2313 96-97 -5.614590325114378 2313 98-99 -4.435358886029494 2313 100-101 -4.2737002902509325 2314 1 -12.029197229302582 2314 2 -14.388339914449293 2314 3 -13.401377187574786 2314 4 -25.513466576455784 2314 5 -18.708374596918183 2314 6 -16.89707246379848 2314 7 -17.019399646439272 2314 8 -11.991113927503676 2314 9 -13.289885683344048 2314 10-11 -11.770465402589533 2314 12-13 -13.188027845399546 2314 14-15 -15.81440716068667 2314 16-17 -16.142765415292985 2314 18-19 -14.341626367186983 2314 20-21 -15.215355907892196 2314 22-23 -14.511577254208568 2314 24-25 -16.02428685965778 2314 26-27 -14.821087873313758 2314 28-29 -16.40480729965224 2314 30-31 -18.04336408124619 2314 32-33 -14.75180108082984 2314 34-35 -15.545316874452336 2314 36-37 -14.810571980370472 2314 38-39 -13.72700571900753 2314 40-41 -17.561809295942712 2314 42-43 -16.67340792886003 2314 44-45 -13.363366732289112 2314 46-47 -13.032092077961881 2314 48-49 -12.80597481010038 2314 50-51 -11.864377099717839 2314 52-53 -11.482690264845662 2314 54-55 -11.609091775575276 2314 56-57 -10.55908112862371 2314 58-59 -9.5775022370653 2314 60-61 -10.486965442480724 2314 62-63 -13.804059870268217 2314 64-65 -10.953065115042964 2314 66-67 -11.425994774631068 2314 68-69 -14.948280013577866 2314 70-71 -14.745787720460509 2314 72-73 -13.281667459687867 2314 74-75 -11.205679039796493 2314 76-77 -11.754694713212835 2314 78-79 -10.999518186316923 2314 80-81 -11.52679188985599 2314 82-83 -11.626033843709628 2314 84-85 -12.134842724664122 2314 86-87 -12.845187826628903 2314 88-89 -11.621661629262594 2314 90-91 -11.222311699166045 2314 92-93 -11.799290337947056 2314 94-95 -11.908617593158555 2314 96-97 -11.832144179485038 2314 98-99 -11.796227677199157 2314 100-101 -11.43161376719592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39329.0 3 6511.0 4 4597.0 5 3885.0 6 4080.0 7 4757.0 8 5599.0 9 6463.0 10 7253.0 11 7930.0 12 8756.0 13 9535.0 14 10696.0 15 12081.0 16 13467.0 17 14423.0 18 13175.0 19 11671.0 20 11728.0 21 12554.0 22 14282.0 23 17408.0 24 21334.0 25 26357.0 26 31642.0 27 36802.0 28 41307.0 29 46882.0 30 53929.0 31 60787.0 32 72089.0 33 87352.0 34 108456.0 35 133698.0 36 165941.0 37 203744.0 38 184907.0 39 46101.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48384770011492 19.816754832237724 14.331698992619202 23.367698475028153 2 14.87757167554182 20.300406638769655 41.23138593692603 23.590635748762487 3 18.144018156121383 25.658585254427123 30.64152866959919 25.555867919852304 4 11.710498461871067 17.556803960464844 39.59371004677122 31.138987530892877 5 12.606204852512862 37.613903180692596 35.597646877686394 14.182245089108147 6 29.940856696551332 37.54006931566961 18.71061573261868 13.808458255160378 7 26.189317727694945 32.62113056260173 22.845618670866806 18.34393303883653 8 24.85367670533149 35.51447462725223 21.10690286953924 18.524945797877045 9 25.454130510595522 16.307093257020945 21.478180708556422 36.76059552382711 10-11 23.7377188153376 26.676289726784823 29.352844932038476 20.233146525839103 12-13 24.709952761077798 24.317588736557692 29.05552946851722 21.91692903384729 14-15 22.087874604805997 25.862181720958894 27.057589488079902 24.99235418615521 16-17 21.377096958978733 28.813489777620493 27.718437467643852 22.090975795756925 18-19 21.04702903155682 27.9463602662749 29.80994471736988 21.1966659847984 20-21 22.05297246948328 27.113541144806142 29.88047170261875 20.95301468309183 22-23 21.739471838795325 27.033936004779324 29.52234345734186 21.704248699083493 24-25 21.494793058779113 27.139230153403982 29.629184509742462 21.73679227807444 26-27 21.33786092686992 27.39551047484265 29.559668978235937 21.706959620051496 28-29 21.27525222171471 27.532754765968697 29.649317659126055 21.542675353190536 30-31 21.690653766687984 27.213697571797006 29.685434789229287 21.410213872285723 32-33 21.54784439186768 27.31420912318667 29.4525421349485 21.685404349997146 34-35 21.73324740202067 27.53046874248502 29.508908960904456 21.227374894589854 36-37 21.544147209303368 27.28658350865426 29.519160049784514 21.650109232257854 38-39 21.54904629313482 27.522702535470454 29.518634112873883 21.409617058520844 40-41 21.710964301729284 27.250281504550067 29.408178552574597 21.630575641146045 42-43 21.5259322842828 27.48307042964104 29.28120924266236 21.709788043413806 44-45 21.61743798930092 27.508149940966607 29.233706848257906 21.640705221474565 46-47 21.57020537024556 27.496845622506022 29.004825548582865 21.928123458665556 48-49 21.613684518542282 27.70482340690591 28.999349067461416 21.68214300709039 50-51 21.537651024218352 27.764261537958664 28.888430988878156 21.809656448944825 52-53 21.519254682272106 27.906117399720536 28.7016229312754 21.87300498673196 54-55 21.49299150656918 27.762195497916466 28.71594021200179 22.028872783512572 56-57 21.58384345034172 27.7938341420045 28.481841676040315 22.140480731613465 58-59 21.534584446957236 27.97915810032895 28.663265830592106 21.82299162212171 60-61 21.797660997251022 27.93744700556594 28.573027452559963 21.691864544623076 62-63 21.648927007294596 27.856025389880806 28.60796238650023 21.887085216324373 64-65 21.46059054782792 27.76874127078761 28.674370039838188 22.09629814154628 66-67 21.634454365390663 27.73521725292607 28.533549953348142 22.096778428335128 68-69 21.554854834919027 27.796725981017783 28.520454133572237 22.12796505049095 70-71 21.611688771599297 27.687156554922076 28.44428064886105 22.25687402461758 72-73 21.672909327617706 27.87094629945433 28.38072269713691 22.07542167579105 74-75 21.557160211505494 27.89560102128183 28.42708638182031 22.120152385392366 76-77 21.604740622720637 27.78609563342463 28.30166749169264 22.30749625216209 78-79 21.58299964074548 27.829865769448254 28.336952561113293 22.250182028692972 80-81 21.694874443427658 27.94247701187333 28.167315267292402 22.195333277406604 82-83 21.796182936755194 27.87040197442697 28.2896283028522 22.043786785965636 84-85 21.801754263505824 27.88154462792823 28.13315086704573 22.183550241520216 86-87 21.580213977370168 28.022716924730094 28.167699496723486 22.229369601176256 88-89 21.755262502345403 27.78948966006582 28.207243109005248 22.24800472858353 90-91 21.82067756298641 27.754012475929628 28.18479770640645 22.240512254677512 92-93 21.886284738342958 27.771751067997723 28.131966159633244 22.209998034026075 94-95 21.713125341403856 27.79269157199147 28.149096388435463 22.34508669816921 96-97 21.67505460860789 27.74850518741751 28.187583369781766 22.388856834192833 98-99 21.60118650048317 27.71222752529991 27.998062202902595 22.68852377131433 100-101 21.814722006804704 27.664454999369227 27.975008437037925 22.545814556788148 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 241.0 1 171.0 2 126.5 3 186.0 4 175.5 5 165.5 6 272.0 7 488.5 8 831.5 9 1083.0 10 1071.0 11 997.5 12 1055.5 13 1184.0 14 1327.0 15 1481.0 16 1633.5 17 1768.5 18 1932.0 19 2211.0 20 2597.0 21 3036.5 22 3659.0 23 4665.0 24 6009.0 25 7597.0 26 9743.0 27 12419.0 28 15399.5 29 18989.0 30 22760.0 31 26304.5 32 30140.5 33 34421.5 34 38477.5 35 41927.5 36 45675.0 37 49509.5 38 52054.0 39 53497.5 40 54471.5 41 55187.5 42 55825.0 43 56513.0 44 57029.0 45 57958.0 46 58142.0 47 57570.5 48 57569.0 49 56930.5 50 55674.5 51 53526.0 52 50352.0 53 47095.0 54 43675.0 55 39835.5 56 35791.5 57 31687.5 58 27498.0 59 23383.0 60 19508.5 61 15712.5 62 12284.5 63 9470.0 64 7207.5 65 5478.0 66 4174.0 67 3209.5 68 2520.0 69 2026.5 70 1604.5 71 1236.0 72 948.5 73 747.0 74 598.0 75 482.5 76 379.0 77 265.5 78 186.0 79 148.0 80 116.0 81 80.5 82 57.0 83 52.0 84 52.5 85 40.5 86 28.0 87 24.5 88 16.0 89 8.0 90 8.5 91 8.0 92 4.0 93 1.0 94 1.0 95 2.0 96 2.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031314698632847644 2 0.2824726700807586 3 0.0 4 0.4742671944271364 5 0.17847457073567768 6 0.09336775175192612 7 0.10361386991400304 8 0.10662366712411316 9 0.10649559064708719 10-11 0.13566500828974998 12-13 0.1718145939303277 14-15 0.12103227078953385 16-17 0.11389200719533647 18-19 0.13614529507859735 20-21 0.10524684499608407 22-23 0.09688985487014005 24-25 0.0920869869816665 26-27 0.13400001408841247 28-29 0.10755222158255137 30-31 0.12003967809258265 32-33 0.09733812253973093 34-35 0.13973143643532426 36-37 0.13102223599755886 38-39 0.12772426671414036 40-41 0.10537492147311002 42-43 0.10556703618864896 44-45 0.09176679578910159 46-47 0.09179881490835808 48-49 0.09589726217318885 50-51 0.08350586302092705 52-53 0.0913825663580237 54-55 0.294511858921199 56-57 0.31365929223658034 58-59 0.3257625193155337 60-61 0.1563173402101863 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1561567.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.6148833668287 #Duplication Level Percentage of deduplicated Percentage of total 1 88.76034566308195 70.6664456756567 2 7.755797135203987 12.349537686720993 3 1.6710480515237887 3.991208871672984 4 0.6318258371519303 2.0121096133199936 5 0.3252303666493544 1.2946588854069643 6 0.19697060058244026 0.9409074835239108 7 0.12774624679804358 0.7119351777563449 8 0.09369090744304993 0.5967352534888611 9 0.0654881295698512 0.4692446817853923 >10 0.35101231800540145 4.83837411922285 >50 0.016108309301316816 0.8618868875356938 >100 0.004409835357475271 0.5830986539568083 >500 8.164983285536375E-5 0.04719393433702813 >1k 2.4494949856609125E-4 0.6366630756155006 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4336 0.27766980219228504 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3539 0.2266313260974393 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1919 0.12288937970641028 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 6.403823851298088E-5 8 0.0 0.0 0.0 0.0 6.403823851298088E-5 9 0.0 0.0 0.0 0.0 6.403823851298088E-5 10-11 0.0 0.0 0.0 0.0 6.403823851298088E-5 12-13 0.0 0.0 0.0 0.0 6.403823851298088E-5 14-15 0.0 0.0 0.0 3.201911925649044E-5 1.2807647702596175E-4 16-17 0.0 0.0 0.0 6.403823851298088E-5 1.2807647702596175E-4 18-19 0.0 0.0 0.0 6.403823851298088E-5 1.2807647702596175E-4 20-21 0.0 0.0 0.0 6.403823851298088E-5 1.2807647702596175E-4 22-23 0.0 0.0 0.0 1.600955962824522E-4 1.2807647702596175E-4 24-25 0.0 0.0 0.0 2.561529540519235E-4 1.2807647702596175E-4 26-27 0.0 0.0 0.0 3.8422943107788523E-4 1.2807647702596175E-4 28-29 0.0 0.0 0.0 0.0017610515591069738 1.2807647702596175E-4 30-31 3.201911925649044E-5 6.403823851298088E-5 0.0 0.005699403227655298 1.2807647702596175E-4 32-33 9.605735776947131E-5 1.2807647702596175E-4 0.0 0.01623369346304065 1.2807647702596175E-4 34-35 1.2807647702596175E-4 2.2413383479543303E-4 0.0 0.0348047826318051 1.2807647702596175E-4 36-37 1.2807647702596175E-4 3.2019119256490434E-4 0.0 0.06413429587075034 1.2807647702596175E-4 38-39 1.9211471553894262E-4 3.2019119256490434E-4 0.0 0.10537492147311002 1.2807647702596175E-4 40-41 1.9211471553894262E-4 3.2019119256490434E-4 0.0 0.14648747059844375 1.2807647702596175E-4 42-43 1.9211471553894262E-4 3.2019119256490434E-4 0.0 0.19208269641968612 1.2807647702596175E-4 44-45 1.9211471553894262E-4 3.8422943107788523E-4 0.0 0.25298306124553094 1.2807647702596175E-4 46-47 1.9211471553894262E-4 4.482676695908661E-4 0.0 0.318077930693976 1.2807647702596175E-4 48-49 1.9211471553894262E-4 4.482676695908661E-4 0.0 0.39002489166331 1.600955962824522E-4 50-51 1.9211471553894262E-4 5.12305908103847E-4 0.0 0.46306050268736465 1.9211471553894262E-4 52-53 1.9211471553894262E-4 5.12305908103847E-4 0.0 0.5380492799860652 2.2413383479543306E-4 54-55 1.9211471553894262E-4 6.083632658733184E-4 0.0 0.624468882859333 2.561529540519235E-4 56-57 1.9211471553894262E-4 8.324971006687513E-4 0.0 0.722991712811554 2.561529540519235E-4 58-59 1.9211471553894262E-4 8.324971006687513E-4 0.0 0.8307040299903878 2.561529540519235E-4 60-61 1.9211471553894262E-4 8.324971006687513E-4 0.0 0.9486304462120421 2.561529540519235E-4 62-63 1.9211471553894262E-4 8.324971006687513E-4 0.0 1.0657243653330277 2.561529540519235E-4 64-65 1.9211471553894262E-4 8.965353391817322E-4 0.0 1.1900225862867235 2.561529540519235E-4 66-67 1.9211471553894262E-4 8.965353391817322E-4 0.0 1.3331800684824922 2.561529540519235E-4 68-69 1.9211471553894262E-4 8.965353391817322E-4 0.0 1.494556429535204 2.561529540519235E-4 70-71 1.9211471553894262E-4 8.965353391817322E-4 0.0 1.6656345837226325 2.561529540519235E-4 72-73 1.9211471553894262E-4 8.965353391817322E-4 0.0 1.8415156057985345 2.561529540519235E-4 74-75 1.9211471553894262E-4 9.285544584382227E-4 0.0 2.0243767958723513 2.561529540519235E-4 76-77 1.9211471553894262E-4 9.605735776947131E-4 0.0 2.2272499354814745 2.561529540519235E-4 78-79 1.9211471553894262E-4 9.605735776947131E-4 0.0 2.4464528259114084 2.561529540519235E-4 80-81 1.9211471553894262E-4 9.605735776947131E-4 0.0 2.6838745951982848 2.881720733084139E-4 82-83 1.9211471553894262E-4 9.605735776947131E-4 0.0 2.9314464252894687 3.2019119256490434E-4 84-85 1.9211471553894262E-4 9.925926969512036E-4 0.0 3.185358040993438 3.2019119256490434E-4 86-87 1.9211471553894262E-4 0.001024611816207694 0.0 3.459121510636431 3.2019119256490434E-4 88-89 1.9211471553894262E-4 0.0012807647702596174 0.0 3.7491506928617215 3.522103118213948E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2160 0.0 57.288956 1 GTATCAA 4090 0.0 41.119156 1 ATCAACG 4705 0.0 34.84886 3 CAACGCA 4770 0.0 34.683304 5 TCAACGC 4790 0.0 34.23817 4 AACGCAG 4970 0.0 33.46387 6 TATCAAC 4940 0.0 33.078976 2 ACGCAGA 5620 0.0 29.093643 7 CGCAGAG 5575 0.0 28.475906 8 GTACTAG 540 0.0 28.312689 1 GAGTACT 3815 0.0 23.672764 12-13 GCAGAGT 6425 0.0 23.59824 9 GTGGTAT 960 0.0 22.893463 1 TATACCG 190 3.2619027E-7 22.518967 5 GTACATG 4170 0.0 22.456646 1 TACATGG 4195 0.0 21.64086 2 CAGAGTA 6165 0.0 21.54819 10-11 ACATGGG 4380 0.0 20.893774 3 GTACTTT 4395 0.0 20.812748 14-15 AGAGTAC 5680 0.0 20.375706 10-11 >>END_MODULE