FastQCFastQC Report
Sat 18 Jun 2016
SRR1931139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561567
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53750.34420553200727216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49520.31711735711628125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29510.18897684185180655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.056.03531
GTATCAA48350.039.557421
ATCAACG57500.032.873663
TCAACGC59550.031.8207244
CAACGCA60000.031.4237635
TATCAAC62100.030.744492
AACGCAG63650.029.6217736
ACGCAGA73650.025.5998087
GTGGTAT12150.025.4527931
CGCAGAG74250.025.2010548
TGGTATC12500.022.7968122
GCAGAGT82200.022.301529
GAGTACT53000.021.64014812-13
TAGACCG1100.002880695321.5871985
CAGAGTA81200.020.49986310-11
GTACTTT56300.020.2030114-15
GTACATG46700.019.968141
AGAGTAC75050.019.55359810-11
ACACCGA2451.7601997E-719.384426
CCGATCG1507.731684E-418.9967359
Produced by FastQC (version 0.11.5)