##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931139_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1561567 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.058801831749776 31.0 31.0 34.0 28.0 34.0 2 31.061697640895332 31.0 31.0 34.0 28.0 34.0 3 31.218999248831462 31.0 31.0 34.0 28.0 34.0 4 34.88041243187132 35.0 35.0 37.0 32.0 37.0 5 34.74400778192675 35.0 35.0 37.0 32.0 37.0 6 34.89460586705533 35.0 35.0 37.0 32.0 37.0 7 34.74327454409577 35.0 35.0 37.0 32.0 37.0 8 34.801568552614135 35.0 35.0 37.0 32.0 37.0 9 36.17038462006433 38.0 35.0 39.0 32.0 39.0 10-11 36.01753110817531 37.0 35.0 39.0 30.5 39.0 12-13 35.95230944301461 37.0 35.0 39.0 30.0 39.0 14-15 36.83356365753119 38.0 36.0 40.0 31.0 41.0 16-17 36.87589677548257 38.0 36.0 40.0 31.0 41.0 18-19 36.94078896390613 38.5 36.0 40.0 31.0 41.0 20-21 36.929312350991026 39.0 36.0 40.0 31.0 41.0 22-23 36.807370737214605 39.0 35.5 40.0 30.5 41.0 24-25 36.66788584799755 38.0 35.0 40.0 30.0 41.0 26-27 36.3784195618888 38.0 35.0 40.0 30.0 41.0 28-29 36.0953926408537 38.0 34.0 40.0 29.0 41.0 30-31 35.85673397298996 38.0 34.0 40.0 29.0 41.0 32-33 35.682580062206746 38.0 34.0 40.0 28.5 41.0 34-35 35.43833565898869 38.0 34.0 40.0 27.0 41.0 36-37 35.67965319451551 38.0 34.0 40.0 29.0 41.0 38-39 35.6570874000283 38.0 34.0 40.0 28.5 41.0 40-41 35.48149102792259 38.0 34.0 40.0 27.0 41.0 42-43 35.310165365943305 38.0 34.0 40.0 27.0 41.0 44-45 35.06410003541315 38.0 33.5 40.0 26.5 41.0 46-47 34.8347646946945 38.0 33.0 40.0 25.5 41.0 48-49 34.562237483245994 38.0 33.0 40.0 24.5 41.0 50-51 34.18871972832417 38.0 33.0 40.0 24.0 41.0 52-53 34.058214601102605 37.5 33.0 40.0 24.0 41.0 54-55 33.814327851446656 37.0 32.5 40.0 23.0 41.0 56-57 33.60164853637404 37.0 32.0 40.0 23.0 41.0 58-59 33.358775512033745 37.0 32.0 40.0 22.0 41.0 60-61 33.0731844999286 36.5 31.0 39.5 22.0 41.0 62-63 32.794165732242035 36.0 31.0 39.0 20.5 40.5 64-65 32.54332827217788 36.0 31.0 39.0 20.0 40.0 66-67 32.29813610302984 35.5 31.0 39.0 19.5 40.0 68-69 31.978366282074354 35.0 30.5 38.5 18.5 40.0 70-71 31.170012878089764 35.0 29.0 38.0 12.5 40.0 72-73 31.091924970238228 34.0 30.0 37.5 15.0 40.0 74-75 30.77876005320297 34.0 29.0 37.0 13.0 39.0 76-77 30.074718535932178 34.0 29.0 36.0 11.5 39.0 78-79 30.106619184447418 34.0 29.0 36.0 9.0 39.0 80-81 29.820908741027445 34.0 29.0 36.0 7.0 37.5 82-83 29.447241777009886 34.0 29.0 35.0 7.0 37.0 84-85 29.075162641116265 34.0 29.0 35.0 2.0 37.0 86-87 28.72468392326426 33.5 29.0 35.0 2.0 36.0 88-89 28.389557732713357 33.0 28.0 35.0 2.0 36.0 90-91 28.057893129145274 33.0 27.0 35.0 2.0 35.5 92-93 27.690442997322563 33.0 27.0 35.0 2.0 35.0 94-95 27.268530905174096 33.0 26.0 35.0 2.0 35.0 96-97 26.870133013825217 33.0 25.5 35.0 2.0 35.0 98-99 26.35945399717079 33.0 25.0 35.0 2.0 35.0 100-101 25.646492593657527 32.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6692299853695332 1101 2 0.5447647420944826 1101 3 0.3795712798021924 1101 4 0.7866998674891903 1101 5 0.811408704813104 1101 6 0.8086636627924975 1101 7 0.5528157309998534 1101 8 0.5434918273615494 1101 9 0.9752390415441923 1101 10-11 0.7173041309793291 1101 12-13 0.8876377180734352 1101 14-15 1.1971163777439244 1101 16-17 0.9032748674746074 1101 18-19 0.8624803797849054 1101 20-21 0.8026081775044176 1101 22-23 0.6552041566136566 1101 24-25 0.5381243853018418 1101 26-27 0.8534188678744883 1101 28-29 0.7353992319390983 1101 30-31 1.012652911084821 1101 32-33 0.8541664017879427 1101 34-35 0.8118833684705251 1101 36-37 0.8873152911595383 1101 38-39 0.7870652455959188 1101 40-41 0.7903323782288325 1101 42-43 0.5836936276994962 1101 44-45 0.5623578735492529 1101 46-47 0.6407494661554978 1101 48-49 0.5171206796883681 1101 50-51 0.561835713258553 1101 52-53 0.5461503869166222 1101 54-55 0.5720466116168765 1101 56-57 0.3831332294028371 1101 58-59 0.6907082290556943 1101 60-61 0.7709653102991751 1101 62-63 0.5235501906310489 1101 64-65 0.5892227446268095 1101 66-67 0.26582136527116873 1101 68-69 0.26531091791041206 1101 70-71 0.594326648269071 1101 72-73 0.22286367492396053 1101 74-75 -0.2556368439100929 1101 76-77 -0.16391269311397494 1101 78-79 -0.00865387151523933 1101 80-81 0.030673500156702005 1101 82-83 -0.036242452917171875 1101 84-85 0.20978438711694025 1101 86-87 -0.004568180892754015 1101 88-89 -0.05359887107075423 1101 90-91 -0.14051873548279303 1101 92-93 -0.3609480981625808 1101 94-95 -0.3500186332740043 1101 96-97 -0.3648105270535247 1101 98-99 -0.4738720406198773 1101 100-101 -0.5793910434182585 1102 1 0.8905503198423332 1102 2 0.7808098663169076 1102 3 0.48095867407456794 1102 4 0.9479773084762257 1102 5 0.9373132719710142 1102 6 0.9408861718892894 1102 7 0.8257547609736591 1102 8 0.875819539100803 1102 9 1.2430636257768768 1102 10-11 1.1108695573151053 1102 12-13 1.181372446305673 1102 14-15 1.7487159329735888 1102 16-17 1.344487727258354 1102 18-19 1.2959926540002797 1102 20-21 1.30910753892163 1102 22-23 0.9092199301250403 1102 24-25 0.5927162947504598 1102 26-27 0.9605035222652205 1102 28-29 1.0381912329580842 1102 30-31 1.3453680411939146 1102 32-33 1.5328067540240156 1102 34-35 1.4303854012637345 1102 36-37 1.5586610656692415 1102 38-39 1.3511262879145107 1102 40-41 1.4616488226824558 1102 42-43 1.5250647733962097 1102 44-45 1.518290565890048 1102 46-47 1.4456254847036547 1102 48-49 1.2074970476497597 1102 50-51 0.9473187813476542 1102 52-53 0.9292396817903992 1102 54-55 0.8636947060238569 1102 56-57 0.8674936310731383 1102 58-59 1.2013196505650896 1102 60-61 1.4282490441667903 1102 62-63 1.0691644177737416 1102 64-65 1.277051735252897 1102 66-67 0.8503833594395864 1102 68-69 0.7673123155633235 1102 70-71 1.045043892069998 1102 72-73 0.7410912720281324 1102 74-75 0.6658651854406195 1102 76-77 0.5587455325553314 1102 78-79 0.7401247657387486 1102 80-81 0.569632558565516 1102 82-83 0.5482767166153693 1102 84-85 0.6057939159426695 1102 86-87 0.7116512712686145 1102 88-89 0.5713249293058524 1102 90-91 0.5140776492091206 1102 92-93 0.5417588732337322 1102 94-95 0.5907706619440276 1102 96-97 0.5008857691662705 1102 98-99 0.4523931622979802 1102 100-101 0.38956667241756193 1103 1 0.7056063734021265 1103 2 0.5451543461726089 1103 3 0.41208606794510416 1103 4 0.7465054411689493 1103 5 0.6996338540422187 1103 6 0.8036317532383634 1103 7 0.6357438166484997 1103 8 0.6743621786409477 1103 9 1.0184008297425038 1103 10-11 0.8472784064920376 1103 12-13 0.9557892999245183 1103 14-15 1.3249474905019056 1103 16-17 1.148681875093402 1103 18-19 0.9578056889803932 1103 20-21 1.0512897924255782 1103 22-23 0.6725902045676264 1103 24-25 0.38941970995760755 1103 26-27 0.860407237879663 1103 28-29 0.6908904373281075 1103 30-31 1.2262487865634384 1103 32-33 1.0685741566243436 1103 34-35 1.0615895658843542 1103 36-37 1.2293536392545832 1103 38-39 1.0134797350559808 1103 40-41 0.9604847444610272 1103 42-43 1.1871509350833662 1103 44-45 1.0999977522356374 1103 46-47 1.1672178358720657 1103 48-49 0.9583208889474193 1103 50-51 0.8672993319017692 1103 52-53 0.3959648893634906 1103 54-55 0.6719200243239882 1103 56-57 0.7524292970561888 1103 58-59 0.9553898162063277 1103 60-61 1.1291946210225916 1103 62-63 0.7471280030191281 1103 64-65 0.8225044483864039 1103 66-67 0.6579610689624324 1103 68-69 0.5111638193649526 1103 70-71 0.7080244715094288 1103 72-73 0.33550138573999533 1103 74-75 0.3860770110888545 1103 76-77 0.4528287928730066 1103 78-79 0.4460717387181461 1103 80-81 0.3793839201872018 1103 82-83 0.274762882153901 1103 84-85 0.23892948114806245 1103 86-87 0.42184252415313495 1103 88-89 0.30398157597746334 1103 90-91 0.2830447424156155 1103 92-93 0.1562540381528379 1103 94-95 0.13799089963807987 1103 96-97 0.17711533681728397 1103 98-99 0.053384249573358744 1103 100-101 -0.034370331918442076 1104 1 0.41050489197820994 1104 2 0.3543561746649182 1104 3 0.14828621407916387 1104 4 0.5373336619968825 1104 5 0.28507862939643047 1104 6 0.5716914956266237 1104 7 0.44116424978258095 1104 8 0.41342079474738114 1104 9 0.823282343077743 1104 10-11 0.49911962749860095 1104 12-13 0.5711743374319269 1104 14-15 1.0817222750096747 1104 16-17 0.7579667870546984 1104 18-19 0.6202086405250427 1104 20-21 0.5036783349301004 1104 22-23 0.32166391093304014 1104 24-25 0.2894062495778087 1104 26-27 0.16615106073853525 1104 28-29 0.3956817900874512 1104 30-31 0.6408429920654655 1104 32-33 0.8672952439303501 1104 34-35 0.6599712548226719 1104 36-37 0.6979423228470978 1104 38-39 0.23686659621147044 1104 40-41 0.6439300415784004 1104 42-43 0.8268067278152174 1104 44-45 0.7480428181423022 1104 46-47 0.8054373291259793 1104 48-49 0.6662054798780233 1104 50-51 0.5751116379436283 1104 52-53 0.16007122863469903 1104 54-55 0.5644320793745479 1104 56-57 0.6802670276992302 1104 58-59 0.7561407464485015 1104 60-61 0.8910362860829224 1104 62-63 -3.331321841386625E-4 1104 64-65 -1.0605655349918521 1104 66-67 -0.3872819545996684 1104 68-69 0.2156465421411724 1104 70-71 0.6400978695602362 1104 72-73 0.16165994977103182 1104 74-75 2.323855018708798E-4 1104 76-77 -0.2945056860854045 1104 78-79 -0.3481026543158663 1104 80-81 -0.057612356321044444 1104 82-83 -0.2635528395697122 1104 84-85 -0.24108368403691216 1104 86-87 0.10802316724391048 1104 88-89 0.037527491641561994 1104 90-91 0.08442933233700955 1104 92-93 0.32092305157637924 1104 94-95 0.39261770020041453 1104 96-97 0.4934289639190972 1104 98-99 0.17456381028307533 1104 100-101 0.04073669513149625 1105 1 -0.501051570808869 1105 2 -1.891916649625955 1105 3 -0.46219836123706415 1105 4 0.4108066415854381 1105 5 0.726560413381641 1105 6 0.09003005588879631 1105 7 -0.19899788149555775 1105 8 -0.22867311881699948 1105 9 0.48797307543087953 1105 10-11 0.9314741334594459 1105 12-13 0.9299151960745462 1105 14-15 1.4104418525848956 1105 16-17 0.8419411113399065 1105 18-19 0.6435657446386713 1105 20-21 1.0050187055302615 1105 22-23 0.3728623553134369 1105 24-25 -0.16371916336426295 1105 26-27 0.9600928939558813 1105 28-29 0.5315778759315748 1105 30-31 1.0447058330061623 1105 32-33 1.1963572881209785 1105 34-35 0.6213559809060172 1105 36-37 0.8080190687189344 1105 38-39 0.7233984712484443 1105 40-41 1.0086585600964568 1105 42-43 1.458658891725996 1105 44-45 1.5921954169872592 1105 46-47 1.5150238301837433 1105 48-49 1.3664991328091105 1105 50-51 1.085980037005804 1105 52-53 1.158149435613531 1105 54-55 0.550184634424042 1105 56-57 0.6916890837448264 1105 58-59 0.08562556465331994 1105 60-61 0.8314806809568438 1105 62-63 1.3733494646598174 1105 64-65 1.68991072485111 1105 66-67 1.1228149113824664 1105 68-69 1.191271476857004 1105 70-71 0.5011895090330292 1105 72-73 -0.23456975550317338 1105 74-75 7.09038622989766E-4 1105 76-77 0.34633359151611387 1105 78-79 -0.17456332083738602 1105 80-81 -0.06341061343811916 1105 82-83 -0.0897626732854171 1105 84-85 0.029788216773930287 1105 86-87 -0.240919638157024 1105 88-89 -0.6716950698136586 1105 90-91 -0.7489594794344043 1105 92-93 -0.11840986058226832 1105 94-95 -0.25503032340382603 1105 96-97 -0.4249032095058709 1105 98-99 -1.1610919354163478 1105 100-101 -0.670109947783267 1106 1 0.3318391329272643 1106 2 0.30226224102171173 1106 3 -0.3474748079511478 1106 4 0.3999146827436988 1106 5 0.1379268467108048 1106 6 0.5239763494454905 1106 7 0.4462581284187692 1106 8 0.5455545167969404 1106 9 0.8807864456841585 1106 10-11 0.2665419530117745 1106 12-13 0.24493690882644614 1106 14-15 0.083508856965544 1106 16-17 -0.22000977995759996 1106 18-19 0.44942504269965866 1106 20-21 0.48815547034819673 1106 22-23 0.49646015578759517 1106 24-25 0.3472232045728987 1106 26-27 0.8298083853807654 1106 28-29 0.7012818478448111 1106 30-31 0.3346344192224322 1106 32-33 0.256393083071508 1106 34-35 -1.1342750115762925 1106 36-37 -1.0805558940088815 1106 38-39 -0.9033869632006883 1106 40-41 0.4152108798852936 1106 42-43 0.57801172520238 1106 44-45 0.3139813294691649 1106 46-47 -0.10716512143646639 1106 48-49 -0.21798642125603607 1106 50-51 0.4674027488102652 1106 52-53 -0.14204104067964352 1106 54-55 0.5363521450162523 1106 56-57 0.34609942132941285 1106 58-59 0.6596085948491819 1106 60-61 0.853942650394373 1106 62-63 0.6606924506610028 1106 64-65 0.6274260901903546 1106 66-67 0.44110189350763207 1106 68-69 0.4573175635297346 1106 70-71 0.6555135850639608 1106 72-73 -0.020605789110675232 1106 74-75 0.3368896322174919 1106 76-77 0.4304323164265824 1106 78-79 0.3567595072764078 1106 80-81 0.4976129661575719 1106 82-83 0.3408252603046904 1106 84-85 0.6121008782447781 1106 86-87 0.5586492158151017 1106 88-89 0.4814449765375528 1106 90-91 0.6482913289082362 1106 92-93 0.2806716695251694 1106 94-95 0.30553472212070787 1106 96-97 0.8497941921523662 1106 98-99 0.9040954714032026 1106 100-101 0.8907022011501802 1107 1 0.41307404758345356 1107 2 0.4523160882579127 1107 3 0.061125596850185815 1107 4 -1.0796071321516933 1107 5 0.20285064881274195 1107 6 0.3490864792200341 1107 7 0.4220282250732055 1107 8 0.43677768788503357 1107 9 0.9580951121127086 1107 10-11 0.8221946951619827 1107 12-13 0.8028165994889633 1107 14-15 1.0669237729205605 1107 16-17 0.825311192864433 1107 18-19 0.6801949499417006 1107 20-21 -0.0807297565678411 1107 22-23 0.32329055593478984 1107 24-25 0.2639920186348732 1107 26-27 0.6751484347362222 1107 28-29 0.7376449676292083 1107 30-31 1.226835093530532 1107 32-33 1.1500549386635086 1107 34-35 0.9078523330784947 1107 36-37 0.9944277353489568 1107 38-39 0.9289668374941868 1107 40-41 0.4205915446686532 1107 42-43 -0.10622902876716012 1107 44-45 -0.27892206480084525 1107 46-47 -0.01745535533370912 1107 48-49 0.5316881953191697 1107 50-51 -1.1320008727546096 1107 52-53 0.4099601012064724 1107 54-55 1.0266007893647213 1107 56-57 0.7804301461835692 1107 58-59 1.0242862961404313 1107 60-61 1.3439105585239588 1107 62-63 1.3511033131909045 1107 64-65 1.1352921161019722 1107 66-67 0.8391290281852584 1107 68-69 0.9343824291551712 1107 70-71 1.356163540146163 1107 72-73 0.9965867675236311 1107 74-75 0.7838358986287233 1107 76-77 0.7491010720413058 1107 78-79 0.7535744026666507 1107 80-81 1.011572659201267 1107 82-83 1.0858240967714465 1107 84-85 0.8901007645973493 1107 86-87 0.9934636187331378 1107 88-89 1.0293372225355384 1107 90-91 1.051898011377311 1107 92-93 1.0430294749391145 1107 94-95 1.041068350937369 1107 96-97 1.1841259993630224 1107 98-99 1.255272128220426 1107 100-101 1.2141353548123313 1108 1 0.464909144876966 1108 2 0.3740630803536682 1108 3 0.3081122331184325 1108 4 0.6752761058044072 1108 5 0.6495251116265379 1108 6 0.5332928390853837 1108 7 0.49625868311323273 1108 8 0.6349062850043907 1108 9 0.7836590498003133 1108 10-11 0.619569118776667 1108 12-13 0.8230123330092312 1108 14-15 1.0211083057577355 1108 16-17 0.8027334852592531 1108 18-19 0.692297813224144 1108 20-21 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1.591291791992255 2311 84-85 1.7714809094688597 2311 86-87 1.833672186656365 2311 88-89 2.011056017177644 2311 90-91 2.166946497957369 2311 92-93 2.3500761352193322 2311 94-95 2.3840695890117978 2311 96-97 2.3889096724665535 2311 98-99 2.3315411545112994 2311 100-101 2.2959617106719 2312 1 -0.07331030548888151 2312 2 -0.149788967811336 2312 3 -0.2876661271972907 2312 4 0.18614635988057415 2312 5 -1.0682458165292488 2312 6 -0.030149864146743255 2312 7 0.17293812080407633 2312 8 -2.4696653575605687 2312 9 -5.794223072686297 2312 10-11 -0.711568174534392 2312 12-13 -6.166425645904184 2312 14-15 -2.340082424207125 2312 16-17 -3.2795186320421266 2312 18-19 -2.4965510661904986 2312 20-21 -3.7850193821170635 2312 22-23 -2.429812926260759 2312 24-25 -3.3591398260762517 2312 26-27 -3.299413011916016 2312 28-29 -6.444063669002702 2312 30-31 -7.039837311217319 2312 32-33 -1.9174052226585445 2312 34-35 0.6214360106966907 2312 36-37 1.079026725446667 2312 38-39 0.767567729355946 2312 40-41 -2.9174847226275595 2312 42-43 -5.247380907578886 2312 44-45 -4.122545417151816 2312 46-47 -0.3081145788893451 2312 48-49 -1.6201447117567014 2312 50-51 -7.518027005615778 2312 52-53 -2.3621938105254436 2312 54-55 0.20965787507526557 2312 56-57 0.9547087874059415 2312 58-59 1.2330737576133188 2312 60-61 1.4676276875160212 2312 62-63 1.0960841316052239 2312 64-65 1.1422319154330722 2312 66-67 0.9900905666467885 2312 68-69 1.1278820028977137 2312 70-71 1.1881877712432853 2312 72-73 0.8389887283079425 2312 74-75 0.437534501815815 2312 76-77 0.7478247588404408 2312 78-79 0.4454255076571414 2312 80-81 0.5329142225824732 2312 82-83 -0.2886840676983553 2312 84-85 0.04953662924420854 2312 86-87 0.6306152352325824 2312 88-89 0.5118442721781378 2312 90-91 0.9909907387692698 2312 92-93 1.1996536798050101 2312 94-95 1.3536429459927817 2312 96-97 1.4143025683183623 2312 98-99 1.2275653933525632 2312 100-101 1.4253205144949348 2313 1 -0.47808075025215047 2313 2 -0.5041648645114805 2313 3 -0.7296675619175197 2313 4 -1.485553783591449 2313 5 -1.6875428036167648 2313 6 -4.5966132787808895 2313 7 -1.2167677615488657 2313 8 -3.2995792743467938 2313 9 -1.5204784528871542 2313 10-11 -0.9069770697998081 2313 12-13 -3.383176004584236 2313 14-15 -2.375322739845572 2313 16-17 -4.013521501482579 2313 18-19 -3.157454939935114 2313 20-21 -5.656952667626385 2313 22-23 -3.525616369589315 2313 24-25 -0.6148249049858663 2313 26-27 -2.756174130997799 2313 28-29 -3.580182750781759 2313 30-31 -3.700041758850027 2313 32-33 -7.939858594251085 2313 34-35 -1.5811285517136326 2313 36-37 -2.1170277939220554 2313 38-39 -1.6147493438147862 2313 40-41 -1.149227907706468 2313 42-43 -3.2950494872058584 2313 44-45 -5.128481572101599 2313 46-47 -1.813978997827654 2313 48-49 0.8051063642834677 2313 50-51 1.0550181880714504 2313 52-53 0.6998219713970784 2313 54-55 -6.4756900445804035 2313 56-57 -7.869860796525192 2313 58-59 -2.6060941046535433 2313 60-61 -11.223638453086565 2313 62-63 -3.800275265812278 2313 64-65 -2.0022802796888755 2313 66-67 -3.82972650652394 2313 68-69 -6.763832487776604 2313 70-71 -6.118107063763887 2313 72-73 -1.3733319316633619 2313 74-75 -0.48427324567342467 2313 76-77 0.20193164549754172 2313 78-79 -0.9014001738349684 2313 80-81 -0.3561639696700354 2313 82-83 1.2728295621438264 2313 84-85 1.0110323250835194 2313 86-87 0.6165908449886786 2313 88-89 -4.211254723517698 2313 90-91 -0.01822733870562132 2313 92-93 0.4719456453717221 2313 94-95 0.9847763749741283 2313 96-97 1.1477853516756085 2313 98-99 0.4625008309422327 2313 100-101 0.29876025624529134 2314 1 -28.55125148195947 2314 2 -14.6435631198079 2314 3 -5.319128128056029 2314 4 -3.8795363365556668 2314 5 -1.4285077315142232 2314 6 -0.7806973094022212 2314 7 0.27228333334164745 2314 8 -3.2312153790290097 2314 9 -2.079495721891959 2314 10-11 -0.5893698061359345 2314 12-13 0.5442763721293105 2314 14-15 -1.456682896512831 2314 16-17 -1.6303988381391576 2314 18-19 -3.7621371546404774 2314 20-21 -2.239737072112767 2314 22-23 -4.663679392555309 2314 24-25 -1.3231211485322447 2314 26-27 -2.483252642658826 2314 28-29 -2.020008280423916 2314 30-31 -4.234668783952397 2314 32-33 -2.9758208516838778 2314 34-35 -2.76743796955234 2314 36-37 -5.626018358280255 2314 38-39 -8.88918409546157 2314 40-41 -8.110239123658047 2314 42-43 -5.239147760305379 2314 44-45 -4.970479079667918 2314 46-47 -6.0807584174466704 2314 48-49 -5.561192371696592 2314 50-51 -3.172594416521747 2314 52-53 -3.8854548667727613 2314 54-55 -4.803089012000736 2314 56-57 -2.378406283439915 2314 58-59 -1.872250415851692 2314 60-61 -0.48463186594040053 2314 62-63 0.17123977780531163 2314 64-65 -1.5491914422311552 2314 66-67 -0.549416732623282 2314 68-69 -0.07431851234487752 2314 70-71 0.6638049889332827 2314 72-73 -0.18346189427686 2314 74-75 0.7625667044779014 2314 76-77 -1.5657379290084208 2314 78-79 -3.6505545266923676 2314 80-81 -2.4781151891822333 2314 82-83 -2.1382439646498383 2314 84-85 -0.1004150667626611 2314 86-87 0.7490458921668868 2314 88-89 0.37509030051648296 2314 90-91 0.5151534695550168 2314 92-93 1.0293606355800193 2314 94-95 1.2352659601619536 2314 96-97 2.0304629375755567 2314 98-99 1.6995490773371102 2314 100-101 1.8917814419316024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 16.0 4 46.0 5 110.0 6 188.0 7 478.0 8 1166.0 9 2017.0 10 3135.0 11 4580.0 12 6010.0 13 7456.0 14 9259.0 15 11225.0 16 12662.0 17 14281.0 18 12965.0 19 11227.0 20 10714.0 21 11494.0 22 12490.0 23 14302.0 24 17100.0 25 21196.0 26 26479.0 27 33337.0 28 41390.0 29 51138.0 30 61511.0 31 74606.0 32 89116.0 33 106068.0 34 127544.0 35 153753.0 36 184313.0 37 217640.0 38 176631.0 39 33889.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.71655953513403 21.693509952295948 15.787302908876082 22.802627603693935 2 15.390437938301718 22.093448439932452 39.786061052775835 22.730052568989993 3 18.62158003031648 26.458460212343432 31.282264091495055 23.637695665845033 4 13.196504788465418 18.58347234985415 38.32988060055137 29.89014226112906 5 12.18338572748679 38.68987506732046 35.91015656769092 13.216582637501833 6 29.742047571445863 39.018690840674786 17.850787061970443 13.388474525908912 7 25.739593626146046 33.54771200979529 22.728899880696762 17.983794483361905 8 24.715109886415377 36.78087459583867 20.407513734601203 18.096501783144753 9 25.183037295229727 16.35856802814096 21.094708072084003 37.36368660454531 10-11 23.839547070346644 27.36632497997204 28.922390137598963 19.87173781208235 12-13 24.668522067897182 24.838543591149147 28.987837217359232 21.505097123594442 14-15 21.871139694934637 26.541192276732282 26.913670691043034 24.673997337290043 16-17 21.172354436280994 29.493675263373266 27.622606010500988 21.711364289844752 18-19 21.012354897356307 28.28633033356878 29.91869705238392 20.78261771669099 20-21 21.755710770014993 27.394918053468086 30.061534343387127 20.7878368331298 22-23 21.635639072803155 27.21673165480572 29.80326812746427 21.34436114492686 24-25 21.460890951974548 27.40747738674156 29.908298013043556 21.223333648240335 26-27 21.354136813032863 27.55464115598607 29.77458271624705 21.31663931473402 28-29 21.31229744913594 27.7068608486571 29.792560377408638 21.188281324798325 30-31 21.804545464733568 27.356520140925483 29.810068237365233 21.028866156975717 32-33 21.632121407536932 27.43660867836167 29.618542595964716 21.312727318136684 34-35 21.71189430980215 27.584219474943083 29.702089917357487 21.001796297897275 36-37 21.60039268725649 27.5316544864455 29.65080164042348 21.21715118587453 38-39 21.529704496763323 27.64667817014626 29.656179587482107 21.167437745608307 40-41 21.763862254636226 27.406488557562163 29.629623700035896 21.20002548776571 42-43 21.58220813930814 27.537910358679913 29.40714823871607 21.472733263295883 44-45 21.688456357486903 27.625033099987096 29.323050430772117 21.36346011175388 46-47 21.612095278664793 27.630396417921922 29.11636308896583 21.64114521444746 48-49 21.69150389170788 27.767307732337603 29.03779303895871 21.503395336995805 50-51 21.75288093010139 27.796585184520527 28.844263135631245 21.606270749746837 52-53 21.794032720074746 27.880617258888453 28.70934565614015 21.61600436489665 54-55 21.684046323686033 27.814647599493263 28.84096292535583 21.660343151464872 56-57 21.84801166017954 27.83294717954038 28.567535928952825 21.751505231327258 58-59 21.775372330734523 27.983857049588917 28.72446805206498 21.51630256761157 60-61 21.94193210832799 27.93024347477485 28.65972640707722 21.46809800981994 62-63 21.87720938572673 27.82769352938163 28.642636661714228 21.652460423177416 64-65 21.68163881184796 27.764579148385792 28.662491566059806 21.89129047370644 66-67 21.75589543910995 27.75522620330359 28.57201409557904 21.91686426200742 68-69 21.719520328959735 27.827766191907482 28.564768430419296 21.88794504871349 70-71 21.78362011327744 27.612624209401876 28.49057558361448 22.113180093706212 72-73 21.756928414046488 27.834182364048615 28.42682277432434 21.982066447580557 74-75 21.717659286497163 27.87196307257631 28.312423013167372 22.097954627759158 76-77 21.498373108550577 27.89294343438354 28.26801539735407 22.340668059711817 78-79 21.60064217545581 27.91884690186204 28.165874406925862 22.314636515756288 80-81 21.591251841904555 28.027047259700698 28.117021215282378 22.26467968311237 82-83 21.75058219005924 27.983225753981355 28.22298591697216 22.04320613898725 84-85 21.75972012719279 28.002745960546672 27.982381794179112 22.255152118081426 86-87 21.468127147518747 28.106475624551525 28.03888211673024 22.386515111199483 88-89 21.640534367546273 27.87715988919696 28.073956642594872 22.40834910066189 90-91 21.672066226632946 27.81110655010704 28.120575301287758 22.396251921972247 92-93 21.77188990040752 27.815385521803044 28.03110571890669 22.381618858882742 94-95 21.505749559173246 27.940395291999092 27.963353184278873 22.590501964548785 96-97 21.456602024002937 27.810860812815914 28.007331135102802 22.725206028078347 98-99 21.298630772117473 27.806417651326864 27.95015423906062 22.944797337495046 100-101 21.385710177987175 27.95621181197515 27.773280726370576 22.884797283667098 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 327.0 1 228.0 2 145.0 3 181.5 4 172.5 5 171.0 6 284.5 7 565.5 8 976.0 9 1263.5 10 1253.0 11 1142.5 12 1158.5 13 1275.0 14 1390.0 15 1555.0 16 1777.5 17 1945.0 18 2131.0 19 2376.0 20 2733.0 21 3212.5 22 3910.0 23 4908.5 24 6188.5 25 7947.5 26 10275.0 27 12980.5 28 16022.5 29 19615.5 30 23449.0 31 27241.0 32 31322.5 33 35227.0 34 38990.0 35 42422.0 36 46368.5 37 50391.5 38 52659.0 39 53510.5 40 54220.0 41 55137.5 42 55237.5 43 55517.0 44 56285.5 45 57388.0 46 57510.0 47 57236.5 48 57330.5 49 56353.5 50 54812.0 51 52634.0 52 49666.0 53 46636.0 54 43404.5 55 39211.0 56 35129.5 57 31414.5 58 27328.5 59 23269.5 60 19284.5 61 15306.5 62 11897.0 63 9286.5 64 7108.0 65 5289.0 66 3973.5 67 3075.5 68 2468.5 69 1946.0 70 1506.5 71 1170.0 72 937.5 73 761.5 74 551.5 75 425.0 76 344.5 77 248.0 78 181.0 79 131.5 80 98.5 81 81.5 82 57.0 83 50.0 84 45.5 85 30.5 86 19.5 87 12.5 88 10.0 89 8.0 90 3.5 91 2.5 92 1.5 93 2.0 94 2.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16521865536349065 2 0.0 3 0.0025615295405192347 4 1.2807647702596175E-4 5 3.8422943107788523E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.016617922894118536 26-27 0.008292951887431023 28-29 0.0048989252462430365 30-31 0.0020812427516718785 32-33 0.0010566309354641842 34-35 0.0014088412472855793 36-37 0.0012167265317466365 38-39 6.724015043862992E-4 40-41 0.0021452809901848593 42-43 0.0013768221280290886 44-45 6.724015043862992E-4 46-47 0.02958566619299716 48-49 0.015016966931294014 50-51 0.083697977736466 52-53 0.0011526882932336557 54-55 0.03807073279596713 56-57 8.324971006687513E-4 58-59 0.0011847074124901461 60-61 0.003201911925649044 62-63 0.0030097972101101006 64-65 0.010726404950924296 66-67 0.005411231154346884 68-69 0.002465472182749764 70-71 0.0058274797046812595 72-73 0.005155078200294961 74-75 0.001120669173977165 76-77 0.0 78-79 0.0 80-81 2.561529540519235E-4 82-83 4.1624855033437565E-4 84-85 3.201911925649044E-5 86-87 0.001697013320593993 88-89 0.0036821987144964002 90-91 0.0012807647702596174 92-93 0.003426045760444477 94-95 8.00477981412261E-4 96-97 5.12305908103847E-4 98-99 0.0019211471553894264 100-101 0.003201911925649044 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1561567.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.65755186173104 #Duplication Level Percentage of deduplicated Percentage of total 1 87.2749352644799 66.90282876266087 2 8.673254680460497 13.297409409548042 3 1.9412647646800858 4.464378131274444 4 0.7417620055071525 2.2744663762490465 5 0.3782966058106822 1.4499645839524595 6 0.21451308201462077 0.9866428625733334 7 0.1408836662949172 0.7559857868831397 8 0.10504302713571713 0.6441873040295565 9 0.07382095783888275 0.5093040513615134 >10 0.4281375146559193 5.7701761372433085 >50 0.020452518000825492 1.0585647713965942 >100 0.007216430200134095 0.9020375768982816 >500 8.389658415300987E-5 0.052801028423505085 >1k 2.516897524590296E-4 0.5855704796540971 >5k 8.389658415300987E-5 0.34568273785181464 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5375 0.34420553200727216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4952 0.31711735711628125 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2951 0.18897684185180655 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.2807647702596175E-4 0.0 0.0 0.0 5 6.403823851298088E-5 1.2807647702596175E-4 0.0 0.0 0.0 6 6.403823851298088E-5 1.2807647702596175E-4 0.0 0.0 6.403823851298088E-5 7 6.403823851298088E-5 1.2807647702596175E-4 0.0 0.0 6.403823851298088E-5 8 6.403823851298088E-5 1.2807647702596175E-4 0.0 0.0 6.403823851298088E-5 9 6.403823851298088E-5 2.561529540519235E-4 0.0 0.0 6.403823851298088E-5 10-11 6.403823851298088E-5 2.561529540519235E-4 0.0 0.0 1.2807647702596175E-4 12-13 6.403823851298088E-5 2.561529540519235E-4 0.0 0.0 1.2807647702596175E-4 14-15 6.403823851298088E-5 3.8422943107788523E-4 0.0 3.201911925649044E-5 1.600955962824522E-4 16-17 6.403823851298088E-5 3.8422943107788523E-4 0.0 6.403823851298088E-5 1.9211471553894262E-4 18-19 6.403823851298088E-5 4.482676695908661E-4 0.0 6.403823851298088E-5 2.5615295405192345E-4 20-21 6.403823851298088E-5 5.12305908103847E-4 0.0 6.403823851298088E-5 3.2019119256490434E-4 22-23 6.403823851298088E-5 5.443250273603374E-4 0.0 1.2807647702596175E-4 3.2019119256490434E-4 24-25 9.605735776947131E-5 5.763441466168278E-4 0.0 2.561529540519235E-4 3.2019119256490434E-4 26-27 1.2807647702596175E-4 5.763441466168278E-4 0.0 3.8422943107788523E-4 3.2019119256490434E-4 28-29 1.2807647702596175E-4 5.763441466168278E-4 0.0 0.0017290324398504834 3.8422943107788523E-4 30-31 1.2807647702596175E-4 5.763441466168278E-4 0.0 0.005635364989142317 3.8422943107788523E-4 32-33 1.2807647702596175E-4 6.403823851298087E-4 0.0 0.016425808178579596 3.8422943107788523E-4 34-35 1.2807647702596175E-4 7.044206236427896E-4 0.0 0.035797375328756306 3.8422943107788523E-4 36-37 1.2807647702596175E-4 7.684588621557705E-4 0.0 0.06599140478762679 3.8422943107788523E-4 38-39 1.2807647702596175E-4 7.684588621557705E-4 0.0 0.10947336873794081 3.8422943107788523E-4 40-41 1.2807647702596175E-4 9.605735776947131E-4 0.0 0.15285927533048535 3.8422943107788523E-4 42-43 1.2807647702596175E-4 9.605735776947131E-4 0.0 0.20011949535306522 3.8422943107788523E-4 44-45 1.2807647702596175E-4 0.0011206691739771653 0.0 0.26300504557281246 3.8422943107788523E-4 46-47 1.2807647702596175E-4 0.0012807647702596174 0.0 0.33146192254318896 3.8422943107788523E-4 48-49 1.2807647702596175E-4 0.0012807647702596174 0.0 0.4069309866307369 4.1624855033437565E-4 50-51 1.2807647702596175E-4 0.0013768221280290888 0.0 0.4834887007730056 4.482676695908661E-4 52-53 1.2807647702596175E-4 0.0014088412472855793 0.0 0.5603025678693261 4.482676695908661E-4 54-55 1.2807647702596175E-4 0.0014088412472855793 0.0 0.6483551458246748 4.482676695908661E-4 56-57 1.2807647702596175E-4 0.0014088412472855793 0.0 0.7486710464552594 4.482676695908661E-4 58-59 1.2807647702596175E-4 0.0014088412472855793 0.0 0.8575680710465834 4.482676695908661E-4 60-61 1.2807647702596175E-4 0.0014088412472855793 0.0 0.9732851680395398 4.482676695908661E-4 62-63 1.2807647702596175E-4 0.0014088412472855793 0.0 1.0898667812524214 4.482676695908661E-4 64-65 1.2807647702596175E-4 0.00147287948579856 0.0 1.2115074153078287 4.482676695908661E-4 66-67 1.2807647702596175E-4 0.00147287948579856 0.0 1.3512388517431528 4.482676695908661E-4 68-69 1.2807647702596175E-4 0.00147287948579856 0.0 1.507876383145904 4.802867888473566E-4 70-71 1.2807647702596175E-4 0.00147287948579856 0.0 1.6743117650411414 5.443250273603374E-4 72-73 1.2807647702596175E-4 0.0015048986050550505 0.0 1.8517297048413548 5.763441466168278E-4 74-75 1.2807647702596175E-4 0.001536917724311541 0.0 2.035903678804688 6.083632658733183E-4 76-77 1.2807647702596175E-4 0.0016009559628245218 0.0 2.2386487419367853 6.403823851298087E-4 78-79 1.2807647702596175E-4 0.0017610515591069738 0.0 2.4574353838163843 6.403823851298087E-4 80-81 1.2807647702596175E-4 0.0017930706783634645 0.0 2.6948891722225174 7.3643974289928E-4 82-83 1.2807647702596175E-4 0.0018571089168764453 0.0 2.942076772882624 8.324971006687513E-4 84-85 1.2807647702596175E-4 0.0018571089168764453 0.0 3.1951238723666675 8.324971006687513E-4 86-87 1.2807647702596175E-4 0.0018571089168764453 0.0 3.4680868640282485 8.324971006687513E-4 88-89 1.2807647702596175E-4 0.0019211471553894262 0.0 3.7564510520522014 8.324971006687513E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2700 0.0 56.0353 1 GTATCAA 4835 0.0 39.55742 1 ATCAACG 5750 0.0 32.87366 3 TCAACGC 5955 0.0 31.820724 4 CAACGCA 6000 0.0 31.423763 5 TATCAAC 6210 0.0 30.74449 2 AACGCAG 6365 0.0 29.621773 6 ACGCAGA 7365 0.0 25.599808 7 GTGGTAT 1215 0.0 25.452793 1 CGCAGAG 7425 0.0 25.201054 8 TGGTATC 1250 0.0 22.796812 2 GCAGAGT 8220 0.0 22.30152 9 GAGTACT 5300 0.0 21.640148 12-13 TAGACCG 110 0.0028806953 21.587198 5 CAGAGTA 8120 0.0 20.499863 10-11 GTACTTT 5630 0.0 20.20301 14-15 GTACATG 4670 0.0 19.96814 1 AGAGTAC 7505 0.0 19.553598 10-11 ACACCGA 245 1.7601997E-7 19.38442 6 CCGATCG 150 7.731684E-4 18.996735 9 >>END_MODULE