FastQCFastQC Report
Sat 18 Jun 2016
SRR1931138_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931138_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2511074
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47960.19099397309677055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36560.1455950720687642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30500.045.0822831
GTATCAA55700.041.3726841
ATCAACG62400.035.521553
CAACGCA63400.034.699885
TCAACGC66150.033.6583634
TATCAAC67500.033.501332
AACGCAG66200.033.360066
ACGCAGA75250.028.9104377
CGCAGAG76400.027.7893128
GCAGAGT85900.023.4418689
GTACATG61350.022.959191
TACATGG61150.021.9374142
GAGTACT51200.020.96796612-13
ACATGGG62750.019.945553
AGAGTAC75450.019.6534810-11
CAGAGTA84200.019.307210-11
GTACTTT60300.018.49682814-15
GTGGTAT18000.018.3654021
TGGTATC17900.017.3973922
GTATAGG7550.017.1332861
Produced by FastQC (version 0.11.5)