##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931138_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2511074 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.826175970919216 31.0 28.0 31.0 23.0 34.0 2 28.529518046859632 31.0 28.0 33.0 16.0 34.0 3 28.5881598869647 31.0 28.0 34.0 16.0 34.0 4 31.740826435222537 35.0 32.0 37.0 19.0 37.0 5 31.868724697081806 35.0 32.0 37.0 19.0 37.0 6 32.13740176514113 35.0 32.0 37.0 23.0 37.0 7 31.795479145576753 35.0 32.0 37.0 18.0 37.0 8 31.967722974312984 35.0 32.0 37.0 19.0 37.0 9 33.00849198390808 37.0 32.0 39.0 18.0 39.0 10-11 32.949422836602984 37.0 32.0 39.0 18.0 39.0 12-13 33.017736832924875 37.0 32.0 39.0 19.0 39.0 14-15 33.78478033701914 38.0 32.0 40.0 18.0 41.0 16-17 33.75605378415769 38.0 32.0 40.0 18.0 41.0 18-19 33.76663332104111 38.0 32.0 40.0 18.5 41.0 20-21 33.97346235116926 38.0 32.0 40.0 22.0 41.0 22-23 33.869042091153034 38.0 32.0 40.0 20.5 41.0 24-25 33.77191552299932 38.0 32.0 40.0 20.0 41.0 26-27 33.388625146053045 38.0 31.5 40.0 17.5 41.0 28-29 33.35154579275641 37.5 31.0 40.0 18.0 41.0 30-31 33.092152401721336 37.0 31.0 40.0 16.5 41.0 32-33 32.87146714911627 37.0 31.0 40.0 16.5 41.0 34-35 32.8506629036022 37.0 30.5 40.0 16.5 41.0 36-37 32.50345210854001 37.0 30.0 40.0 15.5 41.0 38-39 32.32179577344196 37.0 30.0 40.0 14.5 41.0 40-41 32.17270160098826 36.5 30.0 40.0 9.5 41.0 42-43 32.393838652305746 37.0 30.0 40.0 12.5 41.0 44-45 32.44261977146034 37.0 30.0 40.0 11.0 41.0 46-47 32.21543510864275 37.0 30.0 40.0 9.0 41.0 48-49 31.969451716675813 37.0 30.0 40.0 8.0 41.0 50-51 31.442021222791524 35.5 29.5 39.5 7.0 40.5 52-53 31.380797618867465 36.0 30.0 39.5 6.5 40.5 54-55 31.369917015587752 36.0 29.5 40.0 2.0 41.0 56-57 31.112760914254217 36.0 29.0 40.0 2.0 41.0 58-59 30.930392134998808 35.0 29.0 39.5 2.0 41.0 60-61 30.754339378289927 35.0 29.0 39.0 2.0 41.0 62-63 30.573257100348297 35.0 29.0 39.0 2.0 40.5 64-65 30.205968840424454 35.0 28.0 39.0 2.0 40.0 66-67 29.97369790774784 34.0 28.0 38.0 2.0 40.0 68-69 29.775318250278566 34.0 28.0 38.0 2.0 40.0 70-71 29.46979300490547 34.0 28.0 37.5 2.0 40.0 72-73 29.13359223981452 34.0 28.0 37.0 2.0 40.0 74-75 28.72257189553155 34.0 27.5 36.5 2.0 39.0 76-77 28.403529525613344 34.0 27.0 36.0 2.0 39.0 78-79 27.994766781066588 33.0 26.0 36.0 2.0 38.5 80-81 27.60336752321915 33.0 26.0 35.0 2.0 37.0 82-83 27.275632657579983 33.0 26.0 35.0 2.0 37.0 84-85 26.950311898414782 33.0 26.0 35.0 2.0 36.5 86-87 26.63023949115 33.0 25.0 35.0 2.0 36.0 88-89 26.293112030947714 32.0 25.0 35.0 2.0 36.0 90-91 25.91756276398067 32.0 24.0 35.0 2.0 35.0 92-93 25.557012656735722 32.0 22.5 35.0 2.0 35.0 94-95 25.261316870789152 32.0 20.0 35.0 2.0 35.0 96-97 24.870546029308574 32.0 18.5 34.5 2.0 35.0 98-99 24.493423531126524 32.0 8.5 34.0 2.0 35.0 100-101 23.884724623806388 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.108871706284898 1101 2 5.824431661810234 1101 3 5.406490683058102 1101 4 6.779879567428672 1101 5 6.093877786300954 1101 6 5.769065843630212 1101 7 6.333796716200638 1101 8 5.744693537135522 1101 9 6.387618177958309 1101 10-11 6.43965388438254 1101 12-13 6.233956690265945 1101 14-15 6.484004614208725 1101 16-17 6.484922096338412 1101 18-19 6.589236274651654 1101 20-21 5.874966209183995 1101 22-23 5.986369204735006 1101 24-25 5.943513094083045 1101 26-27 6.086293901937847 1101 28-29 6.015679993497663 1101 30-31 6.073617900638922 1101 32-33 5.824292728296495 1101 34-35 5.620340418255214 1101 36-37 5.692808892672421 1101 38-39 5.47253592853566 1101 40-41 5.709707657845911 1101 42-43 5.667963460449712 1101 44-45 5.696582628153141 1101 46-47 5.513853047039472 1101 48-49 5.37326582656063 1101 50-51 5.274570274405246 1101 52-53 5.015463548772718 1101 54-55 5.334160490151017 1101 56-57 5.19992471300413 1101 58-59 5.021541833049852 1101 60-61 5.022772845766774 1101 62-63 4.937415262524976 1101 64-65 4.975312684394826 1101 66-67 4.918632161151233 1101 68-69 4.992926687005703 1101 70-71 4.886609973127349 1101 72-73 4.6400901056969985 1101 74-75 4.778499911356132 1101 76-77 4.670561087253901 1101 78-79 4.637532996023008 1101 80-81 4.511862396820465 1101 82-83 4.4106806216485275 1101 84-85 4.294758468083458 1101 86-87 4.178563797194563 1101 88-89 4.155512162375942 1101 90-91 4.223990865691466 1101 92-93 4.112456742643314 1101 94-95 3.9231160483975707 1101 96-97 3.892513319648671 1101 98-99 3.540653667094123 1101 100-101 3.5752780439185656 1102 1 5.118080977154932 1102 2 5.68571113039911 1102 3 5.325656932505765 1102 4 6.8013764090016835 1102 5 6.362944016255785 1102 6 5.734296236711568 1102 7 6.3091986597853875 1102 8 5.934166140658977 1102 9 6.672369731769308 1102 10-11 6.583485442782578 1102 12-13 6.478605319503661 1102 14-15 6.785769733252952 1102 16-17 6.7557857223341 1102 18-19 6.8049764890004525 1102 20-21 6.093639921612983 1102 22-23 6.143095656962409 1102 24-25 5.9675473960428675 1102 26-27 6.171363612561979 1102 28-29 6.008401312159968 1102 30-31 6.323691281433469 1102 32-33 6.015744464990373 1102 34-35 6.019702597062537 1102 36-37 6.124354598995165 1102 38-39 6.002425693772889 1102 40-41 6.046394346501934 1102 42-43 6.123700180259597 1102 44-45 5.99233383869127 1102 46-47 5.8743129271547865 1102 48-49 5.561398354310786 1102 50-51 5.5714648233014366 1102 52-53 5.48444393789671 1102 54-55 5.717868250213382 1102 56-57 5.60245308276555 1102 58-59 5.600733165387286 1102 60-61 5.482939850957479 1102 62-63 5.423706056706433 1102 64-65 5.42096340809454 1102 66-67 5.381738167039487 1102 68-69 5.39711858091162 1102 70-71 5.199194293200577 1102 72-73 5.016677855785304 1102 74-75 5.17172765172365 1102 76-77 5.053759440629573 1102 78-79 4.974566048059529 1102 80-81 4.992352150871557 1102 82-83 4.914691049786832 1102 84-85 4.900869961362652 1102 86-87 4.899044403332741 1102 88-89 4.9226468228802815 1102 90-91 4.8233813264444585 1102 92-93 4.72604290089226 1102 94-95 4.728316157485075 1102 96-97 4.704388631243802 1102 98-99 4.373798479249093 1102 100-101 4.427458972389516 1103 1 4.953932936376983 1103 2 5.625768374351598 1103 3 5.336321865590168 1103 4 6.823811014905743 1103 5 6.353121241525724 1103 6 5.650519349314511 1103 7 6.279521461747798 1103 8 5.7644171713868 1103 9 6.331011463199232 1103 10-11 6.387265726272542 1103 12-13 6.525989649048643 1103 14-15 6.771570501220207 1103 16-17 6.593189950419291 1103 18-19 6.507222175626794 1103 20-21 5.854123982968595 1103 22-23 6.064051670240083 1103 24-25 5.750379268092139 1103 26-27 6.080176246512178 1103 28-29 5.990387229984108 1103 30-31 6.147320662906921 1103 32-33 5.868937673147801 1103 34-35 5.802892704417541 1103 36-37 5.8974371655954165 1103 38-39 5.654295531142548 1103 40-41 5.760095991360771 1103 42-43 5.699564847572475 1103 44-45 5.507920405972758 1103 46-47 5.45277034583847 1103 48-49 5.339165716999826 1103 50-51 5.277518141859137 1103 52-53 5.107318622522694 1103 54-55 5.453127841588898 1103 56-57 5.399962229325077 1103 58-59 5.415666696509721 1103 60-61 5.206251560446567 1103 62-63 5.298986462779805 1103 64-65 5.140837053045644 1103 66-67 5.329727569493208 1103 68-69 5.232692159450128 1103 70-71 5.120417556982154 1103 72-73 4.939401245683673 1103 74-75 5.116186641958418 1103 76-77 5.115312589811687 1103 78-79 5.05957828444274 1103 80-81 5.0030314194254295 1103 82-83 4.8679064627788335 1103 84-85 4.932287732731197 1103 86-87 4.959034203949077 1103 88-89 4.880717088991517 1103 90-91 4.830372669284731 1103 92-93 4.858695486190701 1103 94-95 4.81291264001516 1103 96-97 4.709699594581799 1103 98-99 4.58316202932917 1103 100-101 4.4896904568056755 1104 1 4.544988780378706 1104 2 5.431382654389882 1104 3 4.934887781296105 1104 4 6.481307042326556 1104 5 5.994563993717637 1104 6 5.596608507749703 1104 7 6.138720616652634 1104 8 5.652938948982577 1104 9 6.3498913090678855 1104 10-11 6.170742981375188 1104 12-13 6.1239598333708365 1104 14-15 6.231715088921593 1104 16-17 6.14296144781358 1104 18-19 6.315006465385359 1104 20-21 5.642560673851804 1104 22-23 5.7905597140443135 1104 24-25 5.461822386007647 1104 26-27 5.6711371516556675 1104 28-29 5.65864257172543 1104 30-31 5.914773626997089 1104 32-33 5.6953891666310845 1104 34-35 5.422623342774383 1104 36-37 5.582112874508294 1104 38-39 5.407612805810164 1104 40-41 5.642531406938453 1104 42-43 5.7138460054185245 1104 44-45 5.543546312040508 1104 46-47 5.5269832253295625 1104 48-49 5.368147321103297 1104 50-51 5.235874838792956 1104 52-53 5.178703413745527 1104 54-55 5.3936531251553 1104 56-57 5.225781543461487 1104 58-59 5.05408322283721 1104 60-61 5.0485422191446006 1104 62-63 4.953473779261824 1104 64-65 5.087968548588915 1104 66-67 5.029933270352707 1104 68-69 5.085541084011304 1104 70-71 5.025904580138924 1104 72-73 4.957032481364163 1104 74-75 5.113214155070359 1104 76-77 5.041885493574881 1104 78-79 4.924876066789434 1104 80-81 4.831661431300251 1104 82-83 4.539278764665497 1104 84-85 4.523839965647863 1104 86-87 4.4330473038197304 1104 88-89 4.543570394772015 1104 90-91 4.5418937335951135 1104 92-93 4.524295077575449 1104 94-95 4.398631929993915 1104 96-97 4.2471455850446205 1104 98-99 4.06251996798165 1104 100-101 4.193060562360465 1105 1 4.518955151646715 1105 2 5.150530897116923 1105 3 4.805046702675561 1105 4 6.275417940249554 1105 5 3.549146072919406 1105 6 4.884676156315631 1105 7 5.645516012996431 1105 8 1.1647751545211342 1105 9 0.6599819807432361 1105 10-11 2.125619684675481 1105 12-13 5.517216101111504 1105 14-15 3.6942148701338517 1105 16-17 5.851922752259288 1105 18-19 6.276106125766432 1105 20-21 3.460602363629498 1105 22-23 5.502968962398278 1105 24-25 5.56951586830575 1105 26-27 5.849744931325251 1105 28-29 3.175167564528074 1105 30-31 5.531603051624572 1105 32-33 5.606734080525282 1105 34-35 5.528763824421265 1105 36-37 5.675871448724418 1105 38-39 5.597379695771092 1105 40-41 5.644839542160746 1105 42-43 2.9171602926503155 1105 44-45 5.048799390610359 1105 46-47 5.2670596471330136 1105 48-49 5.142709941776051 1105 50-51 5.2975603976553 1105 52-53 5.2498727723564365 1105 54-55 5.441382923200631 1105 56-57 5.4620452644706035 1105 58-59 5.401716452500363 1105 60-61 5.308629239327999 1105 62-63 5.0166786001968795 1105 64-65 5.046295579829394 1105 66-67 5.128698553053624 1105 68-69 5.224350981304795 1105 70-71 4.909768335429813 1105 72-73 4.731377219624619 1105 74-75 4.931333401329994 1105 76-77 4.7932834717950925 1105 78-79 4.727385275490004 1105 80-81 4.73511854920481 1105 82-83 4.604137922697845 1105 84-85 4.548951909527148 1105 86-87 4.687909521862686 1105 88-89 4.723878109921841 1105 90-91 4.642664228574457 1105 92-93 4.604472546119805 1105 94-95 4.511502533793756 1105 96-97 4.44789269236891 1105 98-99 4.351243527105943 1105 100-101 4.588293615062057 1106 1 4.397553419947748 1106 2 4.673016056979069 1106 3 4.70769242153089 1106 4 6.391049371708888 1106 5 6.18925524112748 1106 6 5.329486268853056 1106 7 6.065907654619451 1106 8 5.612033703753045 1106 9 6.2593809331722525 1106 10-11 6.235004185983247 1106 12-13 6.386273043671007 1106 14-15 6.231505934388576 1106 16-17 6.1211071569556275 1106 18-19 6.38714147824254 1106 20-21 5.759121191356705 1106 22-23 5.804805662021977 1106 24-25 5.7168966025796735 1106 26-27 6.003397019228114 1106 28-29 6.084839870469672 1106 30-31 6.124298063958079 1106 32-33 5.848840493819228 1106 34-35 5.451051160882063 1106 36-37 5.6681709495653685 1106 38-39 5.603580755790606 1106 40-41 5.8048096656853545 1106 42-43 2.6054060374851886 1106 44-45 4.7859874838606835 1106 46-47 4.948504366858391 1106 48-49 4.935372750300051 1106 50-51 5.127170515063906 1106 52-53 4.98974061495829 1106 54-55 5.394308328082349 1106 56-57 5.245315994319888 1106 58-59 5.18101339462687 1106 60-61 5.0751834858332785 1106 62-63 5.0268143433133154 1106 64-65 5.089547055048019 1106 66-67 5.328228688647904 1106 68-69 5.267233127539857 1106 70-71 5.062253779443221 1106 72-73 4.868174537236719 1106 74-75 5.074915574491769 1106 76-77 4.962883887467793 1106 78-79 5.048099293483542 1106 80-81 4.9388328797647425 1106 82-83 4.950836355671299 1106 84-85 4.914679884759984 1106 86-87 4.975850751263412 1106 88-89 5.0630081740723725 1106 90-91 5.128997026530737 1106 92-93 5.110174650056923 1106 94-95 5.017442106998562 1106 96-97 5.06744175784954 1106 98-99 4.936724750796582 1106 100-101 4.853832020811112 1107 1 2.993348537300772 1107 2 -0.28763798670497565 1107 3 1.1186393222471551 1107 4 2.7676557705299345 1107 5 2.6829936103773804 1107 6 3.959486910549618 1107 7 4.931581756852779 1107 8 4.439379270625576 1107 9 4.946796378587109 1107 10-11 2.6832582181610114 1107 12-13 4.865782898138651 1107 14-15 3.2966319392521903 1107 16-17 1.6032715330606173 1107 18-19 2.459091483442492 1107 20-21 2.032227246095637 1107 22-23 4.200556081890674 1107 24-25 4.21079373640881 1107 26-27 3.249326118396791 1107 28-29 4.031456691327911 1107 30-31 3.6620947656377822 1107 32-33 4.014390018410591 1107 34-35 4.3986206798530425 1107 36-37 4.741037806380742 1107 38-39 4.545567182802049 1107 40-41 3.3452847866672855 1107 42-43 3.858908151764709 1107 44-45 4.472022396578883 1107 46-47 4.627697466628927 1107 48-49 2.3721217163941297 1107 50-51 4.063755516623889 1107 52-53 4.200383373633915 1107 54-55 4.683130637880172 1107 56-57 3.323086297930864 1107 58-59 2.497315474387541 1107 60-61 3.5133821329599115 1107 62-63 4.264007518317246 1107 64-65 4.52176332006427 1107 66-67 4.744917335265608 1107 68-69 4.649544319391641 1107 70-71 4.305237823326415 1107 72-73 4.248405968419188 1107 74-75 4.4013198388973045 1107 76-77 4.18915328495898 1107 78-79 4.1177249188610965 1107 80-81 4.273893409452054 1107 82-83 4.106850048148182 1107 84-85 4.126710699471207 1107 86-87 4.217403479912065 1107 88-89 4.080449486496725 1107 90-91 4.000501937722145 1107 92-93 4.033798044985751 1107 94-95 4.264234398673988 1107 96-97 4.276204788161955 1107 98-99 4.110691010834156 1107 100-101 4.239061936262061 1108 1 -2.364938727736707 1108 2 -1.6999931500793757 1108 3 -2.293854072666136 1108 4 -4.24490020169441 1108 5 -9.175194867082173 1108 6 -7.463185336075348 1108 7 -6.31969421974949 1108 8 -6.467648648236423 1108 9 -7.953285138932227 1108 10-11 -3.565002605208573 1108 12-13 -2.0621858551372405 1108 14-15 -6.835920912990378 1108 16-17 -5.971470419927154 1108 18-19 -4.557238760587698 1108 20-21 -4.854730892490544 1108 22-23 -3.727253207624088 1108 24-25 -9.002395275854 1108 26-27 -9.705815881639456 1108 28-29 -6.7588371803953535 1108 30-31 -6.56885406772712 1108 32-33 -3.0116613852436274 1108 34-35 -1.4316140223752534 1108 36-37 -5.142320093551707 1108 38-39 -6.181258957101772 1108 40-41 -3.9141657731704456 1108 42-43 -4.3769531426581665 1108 44-45 -2.060216289844824 1108 46-47 -2.733361802719667 1108 48-49 -1.7332301717101082 1108 50-51 -1.0945376294726366 1108 52-53 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-6.1804334238001 2313 48-49 -4.707708735175455 2313 50-51 -2.2533444138300815 2313 52-53 -2.189008458304503 2313 54-55 -5.7616665994636485 2313 56-57 -4.244813411894313 2313 58-59 -4.609395717727377 2313 60-61 -2.670920847926915 2313 62-63 -4.030340067955809 2313 64-65 -3.92360587344157 2313 66-67 -5.4817463736962395 2313 68-69 -5.50326629956805 2313 70-71 -5.312832154370895 2313 72-73 -5.560780103189241 2313 74-75 -6.900557903974228 2313 76-77 -5.796204877107257 2313 78-79 -6.610015554531554 2313 80-81 -8.23897260651955 2313 82-83 -6.652156163031947 2313 84-85 -6.663422157431839 2313 86-87 -5.410460497641356 2313 88-89 -6.281616750103673 2313 90-91 -6.184688354611005 2313 92-93 -5.258538701698821 2313 94-95 -5.739560656363302 2313 96-97 -6.136100063273329 2313 98-99 -6.589793413252753 2313 100-101 -6.103103343480857 2314 1 -13.246873973108075 2314 2 -14.729775042196069 2314 3 -14.086116325831473 2314 4 -24.990995516071663 2314 5 -18.74076585996594 2314 6 -17.702942275171495 2314 7 -17.863739381636062 2314 8 -13.768371053584378 2314 9 -15.613719968419918 2314 10-11 -14.715319523972628 2314 12-13 -15.634626630221186 2314 14-15 -17.973464811822822 2314 16-17 -18.302972068058757 2314 18-19 -16.994781119110232 2314 20-21 -17.526331043440916 2314 22-23 -17.70358442046411 2314 24-25 -17.966997028726677 2314 26-27 -16.748988843039292 2314 28-29 -17.95993041535423 2314 30-31 -19.033403835140323 2314 32-33 -15.776277000905498 2314 34-35 -16.107492384101633 2314 36-37 -16.269149211570255 2314 38-39 -15.508861695772792 2314 40-41 -18.140181222478848 2314 42-43 -18.795565092382446 2314 44-45 -15.042400680344194 2314 46-47 -13.582070637937315 2314 48-49 -14.090193142659862 2314 50-51 -13.51711780260347 2314 52-53 -13.120661264957306 2314 54-55 -13.01291400071105 2314 56-57 -12.171346675927575 2314 58-59 -11.71295600878767 2314 60-61 -12.360239974746044 2314 62-63 -15.338941939057678 2314 64-65 -13.062275312111005 2314 66-67 -13.713477142927008 2314 68-69 -16.234529293331043 2314 70-71 -16.311844628383366 2314 72-73 -14.123799853708993 2314 74-75 -12.514253330169655 2314 76-77 -13.01517577107815 2314 78-79 -12.667421999437998 2314 80-81 -12.210126452673396 2314 82-83 -12.764656163031944 2314 84-85 -12.837408643918323 2314 86-87 -12.98982640200726 2314 88-89 -12.167895336382259 2314 90-91 -11.720161327583977 2314 92-93 -11.795752838912959 2314 94-95 -11.408219700022347 2314 96-97 -11.459670749344014 2314 98-99 -11.353436864396205 2314 100-101 -10.706039933917449 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81110.0 3 13988.0 4 9469.0 5 7883.0 6 8171.0 7 9363.0 8 10875.0 9 12455.0 10 13588.0 11 15027.0 12 16515.0 13 17720.0 14 19327.0 15 21775.0 16 23274.0 17 24445.0 18 22487.0 19 20807.0 20 20725.0 21 22047.0 22 25683.0 23 31788.0 24 38906.0 25 47501.0 26 56491.0 27 64296.0 28 71987.0 29 80937.0 30 91234.0 31 101612.0 32 118587.0 33 141429.0 34 170455.0 35 206562.0 36 255005.0 37 300097.0 38 255271.0 39 62109.0 40 73.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51779154593856 19.611928003690156 14.168069462911609 23.70221098745968 2 14.899455425171904 20.086980146791483 40.79081214765449 24.22275228038212 3 18.347209202118297 25.0755254524558 30.15486600554185 26.422399339884052 4 11.824497172805243 17.106962235024362 39.18732724423734 31.881213347933063 5 13.04113693335597 37.329524912862404 35.353154430784286 14.276183722997333 6 30.628186894310705 37.25876067081435 18.22823823539729 13.88481419947766 7 26.745832132376712 32.09585965969773 22.53807879630859 18.620229411616968 8 25.171625535009216 35.398912759212365 20.693175801955693 18.736285903822726 9 25.38187202204405 16.313896489918804 21.283056507013146 37.021174981024004 10-11 24.15663853384047 26.367558336237174 28.93342128290554 20.54238184701681 12-13 24.974069864320754 24.040425256221685 28.62956512641827 22.35593975303929 14-15 22.174536761264097 25.657409467398058 26.44896419683091 25.71908957450694 16-17 21.68101918852436 28.625327416904877 27.13597419735515 22.55767919721561 18-19 21.562159376386063 27.742172852186986 29.07327232311116 21.622395448315796 20-21 22.509829201919036 26.968172601662772 28.999833356057508 21.522164840360684 22-23 22.27317127031742 26.854918383531334 28.737116663886358 22.13479368226489 24-25 22.045219634637853 27.008887909182842 28.89174385505736 22.05414860112194 26-27 21.907362080583045 27.144216520021253 28.8385551630778 22.1098662363179 28-29 21.92796426459176 27.190022662538283 28.862932208332694 22.019080864537262 30-31 22.107848052714143 26.99078004867043 28.788539980666588 22.11283191794883 32-33 21.94812522958884 27.217282580986186 28.65422221230064 22.180369977124332 34-35 22.274393699330382 27.163960897181028 28.73376448579346 21.82788091769513 36-37 22.102738666254062 26.91139571645736 28.694563502634345 22.291302114654233 38-39 22.108191751166032 27.119894423729857 28.80091619594455 21.970997629159562 40-41 22.17337771463915 26.922833894810406 28.792581446400284 22.111206944150158 42-43 22.063001266946102 27.12109820068139 28.60326168855451 22.212638843818 44-45 22.195899926560198 27.28477005967856 28.416759421370084 22.102570592391157 46-47 22.225550549114146 27.21678529062871 28.245856133369045 22.311808026888098 48-49 22.21886273493712 27.33567855220936 28.225479839754446 22.219978873099077 50-51 22.076614831773078 27.506265464377726 28.04919266382675 22.367927040022447 52-53 22.13820634291101 27.587577253531094 27.91709931301407 22.35711709054383 54-55 22.13281261390488 27.474261117465655 27.924577969006485 22.46834829962298 56-57 22.149393231205387 27.55536768996371 27.785574066664363 22.50966501216654 58-59 22.07383417877557 27.620867605543758 27.917634812907195 22.387663402773477 60-61 22.25366303909489 27.513449325748713 27.83656421631725 22.39632341883915 62-63 22.115975076799806 27.520335920008733 27.8470686248195 22.51662037837196 64-65 22.00596238900168 27.557969219545104 27.819450960027464 22.616617431425755 66-67 22.087521116462515 27.44172413875497 27.856725847187295 22.61402889759521 68-69 22.083717962911486 27.50285336075321 27.74575739305174 22.667671283283568 70-71 22.088994589566056 27.298160070153248 27.778293272121807 22.834552068158885 72-73 22.1762082678567 27.291250676005568 27.8754628497607 22.657078206377033 74-75 22.05428832443807 27.500663062896592 27.757067294711348 22.68798131795399 76-77 22.11766757968901 27.43268418214676 27.65822512598195 22.791423112182276 78-79 22.1165724307607 27.464662530853335 27.606394713974975 22.81237032441099 80-81 22.091164975623975 27.45189906788888 27.67110805973858 22.78582789674856 82-83 22.224932439267022 27.322950259530383 27.82588247100643 22.62623483019616 84-85 22.252689486649935 27.390471168910196 27.589509508680347 22.76732983575952 86-87 22.04887231519262 27.390710110494553 27.75822217903574 22.80219539527708 88-89 22.150980018908246 27.234063990149238 27.70776568113883 22.907190309803692 90-91 22.164081982450536 27.24863942679507 27.756649146938724 22.830629443815674 92-93 22.20426399222006 27.16184389627705 27.750416753946716 22.88347535755617 94-95 21.961638725103285 27.173213533332753 27.880400975837432 22.984746765726538 96-97 22.004707945683798 27.074550570791622 27.826001941798612 23.09473954172597 98-99 21.905646747168742 27.15356058801931 27.713978162332133 23.226814502479815 100-101 22.079138249211294 27.05147279610238 27.639985918375963 23.22940303631036 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 309.0 1 211.0 2 134.5 3 172.0 4 162.0 5 163.5 6 262.5 7 454.0 8 784.5 9 1036.0 10 1012.0 11 991.5 12 1127.0 13 1278.5 14 1430.0 15 1580.0 16 1776.0 17 2032.0 18 2261.0 19 2615.5 20 3071.5 21 3534.5 22 4240.5 23 5445.0 24 7188.0 25 9519.0 26 12705.5 27 16700.5 28 21222.5 29 26743.5 30 32786.5 31 38907.5 32 45428.0 33 51894.5 34 58180.5 35 63626.0 36 69536.0 37 75518.5 38 78961.0 39 80893.0 40 83020.5 41 84759.5 42 86121.0 43 87906.5 44 89880.0 45 92985.0 46 94023.0 47 93125.0 48 93115.0 49 92258.0 50 90102.0 51 87454.5 52 84614.0 53 81276.0 54 77570.5 55 71832.5 56 65451.5 57 58971.5 58 51993.5 59 45581.0 60 38270.5 61 30580.0 62 23993.5 63 18841.0 64 14411.5 65 10768.5 66 8169.5 67 6196.5 68 4810.0 69 3781.0 70 2997.5 71 2338.0 72 1791.5 73 1435.5 74 1138.0 75 864.5 76 664.0 77 515.5 78 389.0 79 288.5 80 213.5 81 165.5 82 143.5 83 116.5 84 88.5 85 66.0 86 48.5 87 43.5 88 39.0 89 29.5 90 22.5 91 20.5 92 17.5 93 12.0 94 7.5 95 4.0 96 3.0 97 2.0 98 3.0 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02437204160450867 2 0.32671279301207373 3 0.0 4 0.6028097937376597 5 0.31257541593756294 6 0.09406333704223771 7 0.10581129827316918 8 0.10776265454542559 9 0.10521394431227435 10-11 0.17261538289990658 12-13 0.26874954700657966 14-15 0.11835573145196039 16-17 0.1116653670899384 18-19 0.2031202585029354 20-21 0.10879806807764328 22-23 0.09392395445136224 24-25 0.09497927978227644 26-27 0.2070627946448412 28-29 0.10949498103202057 30-31 0.1186942320298008 32-33 0.10009661204727538 34-35 0.1406171223946407 36-37 0.13659493905794892 38-39 0.1319554899616658 40-41 0.10595068086404463 42-43 0.1067272410132079 44-45 0.08826900362155794 46-47 0.09183321558823038 48-49 0.09653240008060296 50-51 0.08291272977220106 52-53 0.09115621443254958 54-55 0.3032367823489073 56-57 0.3226706978766854 58-59 0.33607133839942593 60-61 0.1621218649868542 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2511074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.83713031662899 #Duplication Level Percentage of deduplicated Percentage of total 1 83.0393932214781 57.99232925822163 2 10.655380870653987 14.882824448743564 3 3.000232475285706 6.285828790701306 4 1.2095018104510924 3.3787254221868652 5 0.5923568509074576 2.0684251295386047 6 0.370574454875702 1.552791389830089 7 0.22597347563085954 1.1046937346013745 8 0.16093348543759822 0.8991306235851888 9 0.11886269571352816 0.7470926613298335 >10 0.5776404060328373 7.1452686951801905 >50 0.03509931028470514 1.6914429466167094 >100 0.013295450618155069 1.552926055628986 >500 5.811481771858984E-4 0.27472474533537555 >1k 1.7434445315234353E-4 0.423796098500366 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4796 0.19099397309677055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3656 0.1455950720687642 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.982359739298802E-5 2 0.0 0.0 0.0 0.0 3.982359739298802E-5 3 0.0 0.0 0.0 0.0 3.982359739298802E-5 4 0.0 0.0 0.0 0.0 3.982359739298802E-5 5 0.0 0.0 0.0 0.0 3.982359739298802E-5 6 0.0 0.0 0.0 0.0 3.982359739298802E-5 7 0.0 0.0 0.0 0.0 3.982359739298802E-5 8 0.0 3.982359739298802E-5 0.0 0.0 3.982359739298802E-5 9 3.982359739298802E-5 3.982359739298802E-5 0.0 0.0 3.982359739298802E-5 10-11 3.982359739298802E-5 3.982359739298802E-5 0.0 0.0 3.982359739298802E-5 12-13 3.982359739298802E-5 3.982359739298802E-5 0.0 0.0 3.982359739298802E-5 14-15 3.982359739298802E-5 5.9735396089482024E-5 0.0 0.0 3.982359739298802E-5 16-17 3.982359739298802E-5 7.964719478597604E-5 0.0 0.0 3.982359739298802E-5 18-19 3.982359739298802E-5 7.964719478597604E-5 0.0 1.991179869649401E-5 1.1947079217896406E-4 20-21 3.982359739298802E-5 7.964719478597604E-5 0.0 9.955899348247005E-5 1.991179869649401E-4 22-23 3.982359739298802E-5 7.964719478597604E-5 0.0 1.7920618826844609E-4 1.991179869649401E-4 24-25 3.982359739298802E-5 7.964719478597604E-5 0.0 1.991179869649401E-4 2.3894158435792812E-4 26-27 3.982359739298802E-5 7.964719478597604E-5 0.0 4.977949674123503E-4 2.3894158435792812E-4 28-29 3.982359739298802E-5 7.964719478597604E-5 0.0 0.002090738863131871 2.3894158435792812E-4 30-31 3.982359739298802E-5 1.3938259087545806E-4 0.0 0.0076262189007572055 2.3894158435792812E-4 32-33 3.982359739298802E-5 1.5929438957195207E-4 0.0 0.022360949936162774 2.787651817509161E-4 34-35 3.982359739298802E-5 1.991179869649401E-4 0.0 0.046553785352402996 3.1858877914390415E-4 36-37 3.982359739298802E-5 2.5885338305442214E-4 0.0 0.0820963460256448 3.1858877914390415E-4 38-39 3.982359739298802E-5 3.1858877914390415E-4 0.0 0.1312984006046815 3.1858877914390415E-4 40-41 3.982359739298802E-5 3.1858877914390415E-4 0.0 0.1821730462742237 3.1858877914390415E-4 42-43 3.982359739298802E-5 3.1858877914390415E-4 0.0 0.23647252131956287 3.1858877914390415E-4 44-45 7.964719478597604E-5 3.385005778403982E-4 0.0 0.305885051575541 3.1858877914390415E-4 46-47 1.3938259087545806E-4 3.5841237653689217E-4 0.0 0.3859703059328399 3.1858877914390415E-4 48-49 1.5929438957195207E-4 3.7832417523338616E-4 0.0 0.46930118347766736 3.1858877914390415E-4 50-51 1.5929438957195207E-4 5.575303635018322E-4 0.0 0.553647562756016 3.1858877914390415E-4 52-53 1.5929438957195207E-4 5.973539608948203E-4 0.0 0.6416776247932159 3.1858877914390415E-4 54-55 1.5929438957195207E-4 5.973539608948203E-4 0.0 0.7386679962438383 3.1858877914390415E-4 56-57 1.7920618826844609E-4 6.371775582878083E-4 0.0 0.8557493725792231 3.1858877914390415E-4 58-59 1.991179869649401E-4 6.570893569843024E-4 0.0 0.9827667364641584 3.1858877914390415E-4 60-61 1.991179869649401E-4 6.770011556807964E-4 0.0 1.1145828438349488 3.1858877914390415E-4 62-63 1.991179869649401E-4 6.770011556807964E-4 0.0 1.2459409798357197 3.1858877914390415E-4 64-65 1.991179869649401E-4 6.770011556807964E-4 0.0 1.381659799751023 3.5841237653689217E-4 66-67 2.5885338305442214E-4 6.770011556807964E-4 0.0 1.5413126016995118 3.5841237653689217E-4 68-69 3.1858877914390415E-4 7.367365517702783E-4 0.0 1.7225298816363037 3.5841237653689217E-4 70-71 3.1858877914390415E-4 7.566483504667724E-4 0.0 1.9107362029155652 3.5841237653689217E-4 72-73 3.1858877914390415E-4 7.566483504667724E-4 0.0 2.107464774036926 3.5841237653689217E-4 74-75 3.1858877914390415E-4 7.566483504667724E-4 0.0 2.312954536584744 3.5841237653689217E-4 76-77 3.1858877914390415E-4 7.765601491632664E-4 0.0 2.53407506110931 3.5841237653689217E-4 78-79 3.1858877914390415E-4 7.964719478597604E-4 0.0 2.774470206772082 3.5841237653689217E-4 80-81 3.385005778403982E-4 7.964719478597604E-4 0.0 3.040471925558546 3.982359739298802E-4 82-83 3.5841237653689217E-4 7.964719478597604E-4 0.0 3.311551153012615 4.380595713228682E-4 84-85 3.5841237653689217E-4 7.964719478597604E-4 0.0 3.5800816702335334 4.380595713228682E-4 86-87 3.982359739298802E-4 7.964719478597604E-4 0.0 3.8657761579308296 4.579713700193622E-4 88-89 3.982359739298802E-4 8.761191426457364E-4 0.0 4.179944517764112 4.977949674123503E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3050 0.0 45.082283 1 GTATCAA 5570 0.0 41.372684 1 ATCAACG 6240 0.0 35.52155 3 CAACGCA 6340 0.0 34.69988 5 TCAACGC 6615 0.0 33.658363 4 TATCAAC 6750 0.0 33.50133 2 AACGCAG 6620 0.0 33.36006 6 ACGCAGA 7525 0.0 28.910437 7 CGCAGAG 7640 0.0 27.789312 8 GCAGAGT 8590 0.0 23.441868 9 GTACATG 6135 0.0 22.95919 1 TACATGG 6115 0.0 21.937414 2 GAGTACT 5120 0.0 20.967966 12-13 ACATGGG 6275 0.0 19.94555 3 AGAGTAC 7545 0.0 19.65348 10-11 CAGAGTA 8420 0.0 19.3072 10-11 GTACTTT 6030 0.0 18.496828 14-15 GTGGTAT 1800 0.0 18.365402 1 TGGTATC 1790 0.0 17.397392 2 GTATAGG 755 0.0 17.133286 1 >>END_MODULE