FastQCFastQC Report
Sat 18 Jun 2016
SRR1931138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2511074
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63060.25112760516018245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50960.20294105231466694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.1340462288247977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37000.052.446711
GTATCAA63800.039.2870831
ATCAACG78050.031.6409723
CAACGCA78900.031.2386675
TCAACGC80400.030.7739894
TATCAAC83700.029.7320772
AACGCAG83350.029.4569026
GTGGTAT16300.029.1791231
ACGCAGA95100.025.2181367
TGGTATC18500.024.901152
CGCAGAG96900.024.2105858
GTACATG72500.021.7801021
GAGTACT65050.021.42707612-13
GCAGAGT109200.021.005199
TACATGG74700.020.0902752
AGAGTAC99400.019.51676410-11
CAGAGTA107950.019.29074910-11
GTACTTT73200.019.2684814-15
ACATGGG73450.018.8803673
AGTACTT70200.017.99478312-13
Produced by FastQC (version 0.11.5)