##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931138_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2511074 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.258728336958608 31.0 31.0 34.0 30.0 34.0 2 31.2700354509664 31.0 31.0 34.0 28.0 34.0 3 31.427628576457725 31.0 31.0 34.0 28.0 34.0 4 35.04523004897506 35.0 35.0 37.0 32.0 37.0 5 34.92981130783083 35.0 35.0 37.0 32.0 37.0 6 35.04800336429751 36.0 35.0 37.0 32.0 37.0 7 34.90835276061159 36.0 35.0 37.0 32.0 37.0 8 34.95544615570867 36.0 35.0 37.0 32.0 37.0 9 36.339116648892066 38.0 35.0 39.0 32.0 39.0 10-11 36.21789819814151 38.0 35.0 39.0 31.5 39.0 12-13 36.13955502705217 38.0 35.0 39.0 31.0 39.0 14-15 37.08902684667994 39.0 36.0 40.0 31.0 41.0 16-17 37.131236873146705 39.0 36.0 40.0 31.0 41.0 18-19 37.185118200419424 39.0 36.0 40.0 31.0 41.0 20-21 37.15988895588103 39.0 36.0 40.0 31.0 41.0 22-23 37.03633923173909 39.0 36.0 40.0 31.0 41.0 24-25 36.89993405212272 39.0 36.0 40.0 31.0 41.0 26-27 36.645884589621815 39.0 35.5 40.0 30.0 41.0 28-29 36.35692775282608 38.0 35.0 40.0 30.0 41.0 30-31 36.115834698619 38.0 35.0 40.0 29.5 41.0 32-33 35.96051609789278 38.0 34.0 40.0 29.0 41.0 34-35 35.743294104434995 38.0 34.0 40.0 28.5 41.0 36-37 36.021975855749375 38.0 35.0 40.0 30.0 41.0 38-39 36.02617286467862 38.0 35.0 40.0 29.5 41.0 40-41 35.86476643061893 38.0 35.0 40.0 29.0 41.0 42-43 35.6806595504553 38.0 34.0 40.0 28.0 41.0 44-45 35.44234578511028 38.0 34.0 40.0 27.0 41.0 46-47 35.224583186317886 38.0 34.0 40.0 26.5 41.0 48-49 34.96273009078983 38.0 33.5 40.0 26.0 41.0 50-51 34.61939891058567 38.0 33.0 40.0 25.0 41.0 52-53 34.47452922534342 38.0 33.0 40.0 24.0 41.0 54-55 34.2177852982429 37.5 33.0 40.0 24.0 41.0 56-57 33.98210885063523 37.0 33.0 40.0 23.5 41.0 58-59 33.72638381027401 37.0 32.0 40.0 23.0 41.0 60-61 33.42865801645033 36.5 32.0 39.5 23.0 41.0 62-63 33.15939534239135 36.0 31.5 39.0 22.5 41.0 64-65 32.90878464752532 36.0 31.0 39.0 22.0 40.5 66-67 32.65011345742897 35.5 31.0 39.0 21.5 40.0 68-69 32.31065034323959 35.0 31.0 39.0 20.5 40.0 70-71 31.493350653943292 35.0 30.0 38.0 16.5 40.0 72-73 31.446065906460742 35.0 30.0 37.0 18.5 40.0 74-75 31.129781121544006 34.0 30.0 37.0 18.0 39.0 76-77 30.402498890912813 34.0 29.0 36.0 17.0 39.0 78-79 30.428918064541307 34.0 29.0 36.0 15.0 39.0 80-81 30.170854184305202 34.0 29.0 36.0 12.5 37.5 82-83 29.822401689476294 34.0 29.0 35.0 9.0 37.0 84-85 29.467061504360284 34.0 29.0 35.0 7.0 37.0 86-87 29.13618734453863 34.0 29.0 35.0 4.5 36.0 88-89 28.825958932313426 34.0 29.0 35.0 2.0 36.0 90-91 28.518011615746886 33.5 29.0 35.0 2.0 35.5 92-93 28.172655007379312 33.0 27.5 35.0 2.0 35.0 94-95 27.784637569422486 33.0 27.0 35.0 2.0 35.0 96-97 27.403516981180164 33.0 27.0 35.0 2.0 35.0 98-99 26.912769994034427 33.0 25.5 35.0 2.0 35.0 100-101 26.221087869174703 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8232947740942258 1101 2 0.7532555615763137 1101 3 0.5933656212208049 1101 4 0.8895493969216233 1101 5 0.8983251875709826 1101 6 0.89950515263412 1101 7 0.830839543318362 1101 8 0.9019385854269473 1101 9 1.214285583330124 1101 10-11 1.0436397197869667 1101 12-13 1.1368681466235486 1101 14-15 1.329691711311355 1101 16-17 1.2635614457772206 1101 18-19 0.9986120641539742 1101 20-21 0.9562686168813812 1101 22-23 0.9288524190671197 1101 24-25 0.761221206751614 1101 26-27 1.0791275837222187 1101 28-29 0.9520801918519979 1101 30-31 1.2047547246471524 1101 32-33 1.3927982023263752 1101 34-35 1.1020035159268602 1101 36-37 1.4194204341336487 1101 38-39 1.1472778612993508 1101 40-41 1.1220550924187762 1101 42-43 1.1712000532273166 1101 44-45 0.9992871997293165 1101 46-47 1.0887283210728285 1101 48-49 1.0025277352058808 1101 50-51 1.0160501053222077 1101 52-53 0.9577174498485732 1101 54-55 1.022652505128498 1101 56-57 0.9573318581479384 1101 58-59 0.981484200118814 1101 60-61 1.0205753175363412 1101 62-63 0.806546879914066 1101 64-65 0.8744028391458443 1101 66-67 0.6460059552634263 1101 68-69 0.6039010459200362 1101 70-71 0.8691684557854202 1101 72-73 0.397976879084613 1101 74-75 0.10789981334549026 1101 76-77 0.2248689651972775 1101 78-79 0.3479908244086296 1101 80-81 0.2305965061781663 1101 82-83 0.22046152846201394 1101 84-85 0.26699908950850215 1101 86-87 0.2259464970060776 1101 88-89 0.0636024114516367 1101 90-91 0.14865083498375498 1101 92-93 -0.13807665590645257 1101 94-95 -0.18700062843739929 1101 96-97 -0.18016268335333407 1101 98-99 -0.23154297659307588 1101 100-101 -0.39227154816798304 1102 1 0.8948024173804967 1102 2 0.8439567864890911 1102 3 0.6818174063075659 1102 4 0.9576467742535826 1102 5 1.0231467105537604 1102 6 0.934381796767191 1102 7 0.9262273399000165 1102 8 0.999774066816272 1102 9 1.4051757515398577 1102 10-11 1.2923589290623596 1102 12-13 1.3371031913010327 1102 14-15 1.6555867874035073 1102 16-17 1.52884134733916 1102 18-19 1.334248883568776 1102 20-21 1.2995793261657482 1102 22-23 0.938734441443323 1102 24-25 0.6766942954099378 1102 26-27 1.0026376596176263 1102 28-29 1.0564162995597712 1102 30-31 1.4092530163482806 1102 32-33 1.4816616284187631 1102 34-35 1.3095840271335888 1102 36-37 1.6622285509314523 1102 38-39 1.4139238441550077 1102 40-41 1.4440255962791255 1102 42-43 1.5548768328001472 1102 44-45 1.4667393687932346 1102 46-47 1.4921533913291896 1102 48-49 1.3361196161768802 1102 50-51 1.2417129709617072 1102 52-53 1.2776602769004057 1102 54-55 1.0367519445097813 1102 56-57 1.245617449114718 1102 58-59 1.3293248449230575 1102 60-61 1.5001447776783223 1102 62-63 1.4100463636479503 1102 64-65 1.3917080430992286 1102 66-67 1.0156823425674801 1102 68-69 0.9424239504517296 1102 70-71 1.2710561098266737 1102 72-73 0.6914199300854342 1102 74-75 0.5582169073243186 1102 76-77 0.7373979726592808 1102 78-79 0.6768829425047471 1102 80-81 0.6868498689934697 1102 82-83 0.6969639955190488 1102 84-85 0.7841443406667992 1102 86-87 0.7705994077323659 1102 88-89 0.6755670312803268 1102 90-91 0.6457872126062227 1102 92-93 0.46174466732377795 1102 94-95 0.46022860773750196 1102 96-97 0.5899045706913526 1102 98-99 0.6234916243171611 1102 100-101 0.5520391751367484 1103 1 0.7652801887964884 1103 2 0.5523979702536046 1103 3 0.36723648230478645 1103 4 0.6828647983201108 1103 5 0.8024543285787118 1103 6 0.7569224649545276 1103 7 0.6626194081251384 1103 8 0.6867375877408293 1103 9 0.9871801136616938 1103 10-11 0.8492443433834183 1103 12-13 1.051372096744025 1103 14-15 1.3318568557357366 1103 16-17 1.1354734150474002 1103 18-19 0.9869596930831435 1103 20-21 1.0526832707872202 1103 22-23 0.892901270267366 1103 24-25 0.5626423098489823 1103 26-27 0.8979282357980054 1103 28-29 0.8797483714727434 1103 30-31 1.268732353106678 1103 32-33 1.348680841632678 1103 34-35 1.0846538734275697 1103 36-37 1.3978654326821527 1103 38-39 1.1353870352375708 1103 40-41 1.1712350896308266 1103 42-43 1.3833503410292352 1103 44-45 1.3460985440312854 1103 46-47 1.2216274850410613 1103 48-49 1.101286075963543 1103 50-51 0.9531940818858899 1103 52-53 0.6213770835372827 1103 54-55 0.835444171116599 1103 56-57 1.0102576356454307 1103 58-59 0.9962670150839656 1103 60-61 1.039100053083132 1103 62-63 0.9461954456655661 1103 64-65 0.8722541295892583 1103 66-67 0.7039747968457917 1103 68-69 0.6242167836356778 1103 70-71 1.1218534774104683 1103 72-73 0.5187363034478913 1103 74-75 0.37304221574764895 1103 76-77 0.6442767063487054 1103 78-79 0.5033118544007209 1103 80-81 0.4164355168867786 1103 82-83 0.4551131672847788 1103 84-85 0.60178404499068 1103 86-87 0.4884423627652765 1103 88-89 0.3512424047760909 1103 90-91 0.16362222760622203 1103 92-93 0.08221770652053095 1103 94-95 0.013355201391203053 1103 96-97 0.017941245869959488 1103 98-99 -0.07448204193019237 1103 100-101 -0.2907103154114594 1104 1 0.4733229481038421 1104 2 0.42360191489350285 1104 3 0.2124990307911503 1104 4 0.6339359081717788 1104 5 0.4639598309771884 1104 6 0.5601274973111146 1104 7 0.5603662273872771 1104 8 0.5913785703281604 1104 9 0.8247589339369128 1104 10-11 0.6443579279249079 1104 12-13 0.6533198858454128 1104 14-15 0.9401397486541114 1104 16-17 0.8577630736033797 1104 18-19 0.6936553456248333 1104 20-21 0.6369774080888178 1104 22-23 0.3356705541631655 1104 24-25 0.319078659390172 1104 26-27 0.08507384777423255 1104 28-29 0.5315638656635855 1104 30-31 0.8470219559333501 1104 32-33 0.91229758918562 1104 34-35 0.7836306683902379 1104 36-37 1.0018619207405308 1104 38-39 0.43811941339377825 1104 40-41 0.6913817323504787 1104 42-43 0.8945582762857143 1104 44-45 0.8972808139507151 1104 46-47 0.8658276686989907 1104 48-49 0.6263520492647245 1104 50-51 0.620322820818707 1104 52-53 0.3697431574248924 1104 54-55 0.7313069416743119 1104 56-57 0.7451569459703506 1104 58-59 0.5559850247813571 1104 60-61 0.7223628996217855 1104 62-63 -0.10221257447858534 1104 64-65 -0.8448360093334628 1104 66-67 -0.3225225738006401 1104 68-69 0.21009318548280476 1104 70-71 0.7017043965335574 1104 72-73 0.24069477068291434 1104 74-75 0.24941475523024792 1104 76-77 -0.08270264818150252 1104 78-79 -0.1858441177535326 1104 80-81 -0.05898686275968146 1104 82-83 -0.199677625138726 1104 84-85 -0.22186958839854043 1104 86-87 -0.02369172394206487 1104 88-89 -0.05286203393895761 1104 90-91 0.05577265494304129 1104 92-93 0.1685112820507868 1104 94-95 0.09169610503697001 1104 96-97 0.23206322687770609 1104 98-99 0.23843613624884696 1104 100-101 -0.0612183900658394 1105 1 -0.25285124797946423 1105 2 -1.7659740110916609 1105 3 -0.4642011310212446 1105 4 0.38358213593917156 1105 5 0.5568428015654732 1105 6 -0.008419335638258474 1105 7 -0.2607277475302965 1105 8 -0.16407805628475813 1105 9 0.5246791443111292 1105 10-11 0.8908102168068126 1105 12-13 0.9666153603492447 1105 14-15 1.156946057225099 1105 16-17 0.8874942151563303 1105 18-19 0.6000270180141882 1105 20-21 1.0148183140933682 1105 22-23 0.2989520262515839 1105 24-25 -0.13261668909191826 1105 26-27 0.9717003037689267 1105 28-29 0.48557269080661314 1105 30-31 1.0934267452422617 1105 32-33 1.1272634854578953 1105 34-35 0.633554620789269 1105 36-37 0.9454338463804604 1105 38-39 0.799042293280209 1105 40-41 0.988935625052136 1105 42-43 1.391584875443037 1105 44-45 1.304974718735501 1105 46-47 1.3997659023546447 1105 48-49 1.1482015812400235 1105 50-51 0.7941147956240187 1105 52-53 0.9699107337455928 1105 54-55 0.15712332172400778 1105 56-57 0.39019372343515357 1105 58-59 -0.24944848577506917 1105 60-61 0.3290180002128409 1105 62-63 0.7439700170184409 1105 64-65 1.142497810060732 1105 66-67 0.897991115672859 1105 68-69 0.8912652480084198 1105 70-71 0.2812012692268553 1105 72-73 -0.2350686174670571 1105 74-75 -0.15653177485653558 1105 76-77 0.04569056861391374 1105 78-79 -0.4969364449730804 1105 80-81 -0.34925421836128123 1105 82-83 -0.3188070672088621 1105 84-85 -0.1908760264918712 1105 86-87 -0.5156662576525761 1105 88-89 -0.7795416447925199 1105 90-91 -1.015390637966366 1105 92-93 -0.6420978713040277 1105 94-95 -0.8929598429416465 1105 96-97 -1.0953926428805154 1105 98-99 -1.5689799231938224 1105 100-101 -0.9362607141770951 1106 1 0.45130818523348637 1106 2 0.4300324459074574 1106 3 -0.2282833292153974 1106 4 0.495886426758517 1106 5 0.2714028474626673 1106 6 0.6121442851524108 1106 7 0.5254781971846967 1106 8 0.6020168800044701 1106 9 1.0488006095032816 1106 10-11 0.38358147639522144 1106 12-13 0.6362307954457194 1106 14-15 0.2865090867105238 1106 16-17 0.06846365807101762 1106 18-19 0.6839235571871427 1106 20-21 0.6502789706451537 1106 22-23 0.7676644015802125 1106 24-25 0.5710523125764553 1106 26-27 0.9507056525781294 1106 28-29 0.9090780890040122 1106 30-31 0.4280546356356538 1106 32-33 0.6221917913886799 1106 34-35 -0.9087607113361926 1106 36-37 -0.6170088666957767 1106 38-39 -0.4624830848101027 1106 40-41 0.7261210797550461 1106 42-43 0.9234321427874264 1106 44-45 0.7234141735439508 1106 46-47 0.30468131323060277 1106 48-49 0.23675901294562607 1106 50-51 0.7581001794244173 1106 52-53 0.23663127639332515 1106 54-55 0.9515045011682375 1106 56-57 0.8002755454671586 1106 58-59 1.0513495056814506 1106 60-61 1.1306175688441726 1106 62-63 0.8692820759920039 1106 64-65 0.9796221722426282 1106 66-67 0.6706749107970964 1106 68-69 0.7367567823305059 1106 70-71 1.0813695273660997 1106 72-73 0.39248187688847835 1106 74-75 0.5666748585869854 1106 76-77 0.7273033435133414 1106 78-79 0.7297775621956504 1106 80-81 0.6805500014202437 1106 82-83 0.6019305624013249 1106 84-85 0.7402237877273272 1106 86-87 0.8781627888305117 1106 88-89 0.7820684374354414 1106 90-91 0.9430054905403651 1106 92-93 0.5131236110725084 1106 94-95 0.6574935136289461 1106 96-97 0.9343897243159809 1106 98-99 0.9453279722872274 1106 100-101 0.740560255644688 1107 1 0.4592698693107842 1107 2 0.42852822066754115 1107 3 0.14888245198378058 1107 4 -1.11853094595876 1107 5 0.2201403648050757 1107 6 0.4173876098486531 1107 7 0.5468323096851648 1107 8 0.6809734371475429 1107 9 0.8852217571665051 1107 10-11 0.92914226386754 1107 12-13 0.9641285532694397 1107 14-15 1.038286807448614 1107 16-17 1.136948612499502 1107 18-19 0.8944490402850818 1107 20-21 0.2654612899135387 1107 22-23 0.5618941112457136 1107 24-25 0.5551529527240859 1107 26-27 0.9567830721613788 1107 28-29 0.8507184424239185 1107 30-31 1.178043903813922 1107 32-33 1.340948471117926 1107 34-35 1.0596642776553935 1107 36-37 1.3123755250312072 1107 38-39 0.941763065563741 1107 40-41 0.39232989224024806 1107 42-43 0.2757041911492166 1107 44-45 0.026332565231101057 1107 46-47 0.49464816628429986 1107 48-49 0.6658504370030442 1107 50-51 -0.9680892272717969 1107 52-53 0.6192327806185602 1107 54-55 1.011274331251407 1107 56-57 1.0853350794184422 1107 58-59 0.9933786387051455 1107 60-61 1.271163055172714 1107 62-63 1.4181898641967479 1107 64-65 1.1989571533347103 1107 66-67 1.0205008782652953 1107 68-69 1.1213240907674908 1107 70-71 1.3718710906716751 1107 72-73 1.0031392396926506 1107 74-75 0.9135988086939726 1107 76-77 1.0127413677960249 1107 78-79 0.9307882342732157 1107 80-81 0.9320709328981174 1107 82-83 0.9355827894202875 1107 84-85 0.8710973580163319 1107 86-87 0.9687312877252445 1107 88-89 1.0742151779488438 1107 90-91 1.047426005358023 1107 92-93 1.0219952872502986 1107 94-95 1.0866301883573861 1107 96-97 1.1498751898889594 1107 98-99 0.9876758514801054 1107 100-101 0.8123883384987778 1108 1 0.47387301864455367 1108 2 0.3993833977229535 1108 3 0.18534359666174183 1108 4 0.5757095961078704 1108 5 0.4797951626298982 1108 6 0.45778568825259214 1108 7 0.5639637813819931 1108 8 0.522356299777023 1108 9 0.8213184538591491 1108 10-11 0.5856580331011827 1108 12-13 0.5924716510559946 1108 14-15 0.7551951009500613 1108 16-17 0.7095950606418882 1108 18-19 0.5545008184792408 1108 20-21 0.5394367330273511 1108 22-23 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1.533532114077687 2311 84-85 1.5846847253304084 2311 86-87 1.478064489605032 2311 88-89 1.5486748490958853 2311 90-91 1.739318060086518 2311 92-93 1.748804690560732 2311 94-95 1.9510550243143143 2311 96-97 1.9061466195558339 2311 98-99 2.24412529115936 2311 100-101 2.1750111654073194 2312 1 -0.05237226880383261 2312 2 -0.015321675420523206 2312 3 -0.258913649965244 2312 4 0.24800282012523667 2312 5 -1.1686832767493485 2312 6 0.1233344794279958 2312 7 0.30090699918500263 2312 8 -2.4048651192414496 2312 9 -5.3863131974911695 2312 10-11 -0.7106134077472035 2312 12-13 -5.0863652343736305 2312 14-15 -2.009565051551249 2312 16-17 -2.834587401283251 2312 18-19 -2.50684521054869 2312 20-21 -3.5733545847237096 2312 22-23 -2.024652882133573 2312 24-25 -2.9336679821773757 2312 26-27 -3.3113709199163637 2312 28-29 -5.8604357920757835 2312 30-31 -6.793133772653967 2312 32-33 -1.4222193954982174 2312 34-35 0.7416566801982327 2312 36-37 1.3607863667061295 2312 38-39 1.2533500114942342 2312 40-41 -2.610064318817493 2312 42-43 -4.582980869774012 2312 44-45 -3.508041338868658 2312 46-47 0.4797833459472187 2312 48-49 -0.6761592881186971 2312 50-51 -6.548202035859653 2312 52-53 -1.2130753588752725 2312 54-55 0.9592824700391631 2312 56-57 1.5970607707329094 2312 58-59 1.8221440048446809 2312 60-61 1.949795089234172 2312 62-63 1.8977917037793048 2312 64-65 1.7477839958004466 2312 66-67 1.411938793530581 2312 68-69 1.639240956510445 2312 70-71 2.0776520306569495 2312 72-73 1.596504644902133 2312 74-75 1.5719868438757523 2312 76-77 1.7132843459145022 2312 78-79 1.587579344212756 2312 80-81 1.6073731954246249 2312 82-83 0.6195916484130848 2312 84-85 0.9943236177279218 2312 86-87 1.6591866879686066 2312 88-89 1.5580346889154946 2312 90-91 1.87096047046456 2312 92-93 1.999866634151413 2312 94-95 2.2017029502495973 2312 96-97 2.153531524986718 2312 98-99 1.9648458222247172 2312 100-101 2.1220835693762723 2313 1 -0.35619333124558494 2313 2 -0.12340827661057219 2313 3 -0.2041140227518241 2313 4 -1.9682707478724737 2313 5 -1.626501041463662 2313 6 -0.6328917733499821 2313 7 -1.4284140991016159 2313 8 -4.329067571030109 2313 9 -2.191962347967909 2313 10-11 -0.8864360473629489 2313 12-13 -3.2484628757816196 2313 14-15 -2.5144614598959265 2313 16-17 -6.309116041329126 2313 18-19 -3.900554437016538 2313 20-21 -4.0391191556607 2313 22-23 -2.6038628613435506 2313 24-25 -0.8066015258005734 2313 26-27 -2.4259203499830377 2313 28-29 -3.3085467677729596 2313 30-31 -4.037308982346417 2313 32-33 -7.909630679461223 2313 34-35 -2.535700119572361 2313 36-37 -3.104526558951619 2313 38-39 -1.998582026644705 2313 40-41 -0.3802413924428407 2313 42-43 -1.6617535416501283 2313 44-45 -4.4243077378936775 2313 46-47 -2.3276365407830113 2313 48-49 -1.6998993411690932 2313 50-51 0.5762979282954817 2313 52-53 0.3979576900888162 2313 54-55 -2.6931406427287854 2313 56-57 -4.734360836550771 2313 58-59 -0.9259167880436934 2313 60-61 -6.60035904367858 2313 62-63 -1.7335116154550505 2313 64-65 -3.1148477340726615 2313 66-67 -2.6852258777720195 2313 68-69 -4.5984929312234435 2313 70-71 -3.5219465068726308 2313 72-73 0.35626161672806944 2313 74-75 1.4035589995858366 2313 76-77 -0.9627856232589131 2313 78-79 0.44016375886613446 2313 80-81 1.4619613379438015 2313 82-83 2.132330912876487 2313 84-85 2.0900114806571644 2313 86-87 1.9733740849146244 2313 88-89 -3.0903451232574177 2313 90-91 0.8119311493662735 2313 92-93 1.539442947865659 2313 94-95 1.7707509106768704 2313 96-97 2.14654177661766 2313 98-99 2.6122148092488757 2313 100-101 3.1408770312731846 2314 1 -28.707544682596936 2314 2 -14.858491436693733 2314 3 -5.4535422971801 2314 4 -4.923416278018003 2314 5 -1.8447442847069055 2314 6 -1.3607504012086125 2314 7 -0.3058569265444433 2314 8 -3.743724535687072 2314 9 -4.439363595369155 2314 10-11 -0.7550431159700111 2314 12-13 0.17258702026827422 2314 14-15 -2.443749401683874 2314 16-17 -2.165820823033904 2314 18-19 -5.023423459885564 2314 20-21 -3.0395349560765013 2314 22-23 -6.125614420595113 2314 24-25 -1.5676202368192875 2314 26-27 -3.9166428032054874 2314 28-29 -3.8242952085214057 2314 30-31 -5.1868671944046305 2314 32-33 -3.170727353057895 2314 34-35 -3.0589069802792217 2314 36-37 -6.696574375999436 2314 38-39 -8.957027972590652 2314 40-41 -7.861556361257811 2314 42-43 -5.906919861816451 2314 44-45 -6.005388818974758 2314 46-47 -7.823764399410869 2314 48-49 -6.522976264246015 2314 50-51 -3.2209473939498423 2314 52-53 -4.3457013535702345 2314 54-55 -6.50673211882026 2314 56-57 -2.527110316800247 2314 58-59 -2.4232140853409945 2314 60-61 -0.3985139293334612 2314 62-63 -0.08977460921803981 2314 64-65 -2.5577531394780664 2314 66-67 -1.3821593497054927 2314 68-69 0.5575101872796715 2314 70-71 0.9170087945826744 2314 72-73 0.2772075626740147 2314 74-75 0.6016619101887493 2314 76-77 -1.2741161845894737 2314 78-79 -2.3759485072461324 2314 80-81 -2.3473681838857203 2314 82-83 -2.814758484212909 2314 84-85 -0.8636007854551018 2314 86-87 0.420957244997787 2314 88-89 0.6049511345388403 2314 90-91 1.0748729373080614 2314 92-93 0.9670417004644101 2314 94-95 1.1234536133795707 2314 96-97 1.7654347080105914 2314 98-99 2.0661389256729947 2314 100-101 2.282561022957175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 16.0 4 67.0 5 113.0 6 277.0 7 736.0 8 1873.0 9 3030.0 10 4537.0 11 6215.0 12 8409.0 13 10535.0 14 12775.0 15 15705.0 16 17841.0 17 20119.0 18 18739.0 19 16445.0 20 16405.0 21 16982.0 22 18757.0 23 21297.0 24 25328.0 25 31330.0 26 39181.0 27 49477.0 28 61894.0 29 76966.0 30 94308.0 31 114198.0 32 137816.0 33 166662.0 34 202703.0 35 247856.0 36 309097.0 37 370130.0 38 307772.0 39 65411.0 40 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.40927823870723 20.9795468537179 15.135352172429082 23.47582273514579 2 15.155069105888556 21.33318253464454 39.87644330672852 23.63530505273839 3 18.588643291408037 25.719422025078714 30.5807228589384 25.11121182457485 4 13.077072263136788 17.55562095514283 38.11224666564983 31.25506011607055 5 12.656890477235375 38.143785092154054 35.73333566965756 13.465988760953012 6 30.633147410231636 38.33511079323031 17.540741531312896 13.491000265225159 7 26.607897656540587 32.86972028701663 22.414592321851128 18.107789734591652 8 25.191173179285038 36.36292677953736 20.04030944528118 18.405590595896417 9 25.158557653020182 16.38713156203282 20.971106387147493 37.48320439779951 10-11 24.22903506627045 26.900103302411637 28.693937335180088 20.176924296137827 12-13 24.87330122489421 24.34358764417138 28.758292268567153 22.02481886236726 14-15 22.04791654885519 26.13196584409699 26.376781409070382 25.44333619797744 16-17 21.59556030606824 29.154676445218264 27.064515024248585 22.18524822446491 18-19 21.47146997659169 28.124420068862964 29.139682860799805 21.264427093745546 20-21 22.31531209355041 27.19495721750932 29.095598138485762 21.394132550454508 22-23 22.148132631694644 27.08771226973 28.87981397601186 21.88434112256349 24-25 21.977306121603597 27.237652815047042 29.02491124748221 21.760129815867156 26-27 21.967960880460463 27.335785871658725 28.874456960700446 21.82179628718037 28-29 21.91142102949048 27.310534976932683 29.00260941044529 21.77543458313155 30-31 22.127698636427084 27.166062627699933 28.916570938388027 21.78966779748496 32-33 22.03044035711875 27.349826755153188 28.684930137554478 21.93480275017358 34-35 22.206026892742848 27.247321000457188 28.87041161093615 21.67624049586382 36-37 22.120575356262385 27.140496420699744 28.795530768083527 21.94339745495434 38-39 22.011243888520074 27.23363465446568 28.976449322256443 21.77867213475781 40-41 22.21237449036992 27.06677087429871 28.8615249824523 21.85932965287907 42-43 22.115460051699838 27.200120428524833 28.647652181393074 22.03676733838225 44-45 22.243649683639074 27.332703850491587 28.49496387177347 21.928682594095868 46-47 22.211970772213046 27.320638798126375 28.31991615057521 22.14747427908537 48-49 22.253788501151423 27.394571117830647 28.237779120091467 22.113861260926463 50-51 22.262793505211384 27.500600830521154 28.03626664838601 22.200339015881454 52-53 22.266000141376107 27.47509241716306 27.952923747502272 22.305983693958563 54-55 22.271785548600885 27.458040762195164 27.993348590091127 22.27682509911282 56-57 22.32959696571878 27.49992582753079 27.884826184534333 22.2856510222161 58-59 22.25640237327818 27.534371693976382 27.99430037297273 22.21492555977271 60-61 22.30378379174878 27.54829625726781 27.889297692361147 22.25862225862226 62-63 22.251652833482876 27.515451070911844 27.82819611858426 22.404699977021025 64-65 22.13002958612286 27.476162530311488 27.855622428738506 22.538185454827143 66-67 22.206424544016283 27.42095269956102 27.846673576491458 22.52594917993124 68-69 22.197734495920663 27.42195018455732 27.823024095697008 22.55729122382501 70-71 22.20973235350811 27.26237432913143 27.791163730961195 22.73672958639927 72-73 22.220556181482415 27.311551875132174 27.845116356683526 22.622775586701888 74-75 22.190256759789307 27.425908790508807 27.68227631261665 22.70155813708524 76-77 22.093872980246697 27.46752982986563 27.565655173841954 22.872942016045723 78-79 22.118165374656424 27.461436819464502 27.473961340844593 22.946436465034484 80-81 22.046783299688936 27.528636233123212 27.538492600954097 22.886087866233755 82-83 22.139125513950564 27.376468177726288 27.702505332899786 22.781900975423362 84-85 22.17730341678501 27.46279082177586 27.43507359799034 22.924832163448787 86-87 21.956649696220516 27.442023837211156 27.59903099602076 23.00229547054757 88-89 22.070038740047412 27.32588135848035 27.531798002164503 23.072281899307736 90-91 22.1066435898794 27.26927161965355 27.56345286827713 23.060631922189916 92-93 22.127177436698023 27.219691116615824 27.520589570605896 23.132541876080257 94-95 21.834602323887488 27.255400228828485 27.62584272481921 23.284154722464816 96-97 21.824238299458514 27.19543825931609 27.633540271256074 23.346783169969324 98-99 21.718137761492237 27.2926431121046 27.514365670378705 23.474853456024462 100-101 21.83808780978138 27.203791978803533 27.400049661796 23.558070549619085 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 314.0 1 225.0 2 147.0 3 183.0 4 173.5 5 161.5 6 287.0 7 605.5 8 1004.5 9 1233.5 10 1191.0 11 1131.5 12 1236.5 13 1448.5 14 1663.5 15 1846.5 16 2024.5 17 2236.0 18 2495.5 19 2735.0 20 3141.0 21 3686.5 22 4455.0 23 5746.0 24 7506.0 25 9925.0 26 13206.0 27 17189.0 28 21828.0 29 27651.5 30 33752.0 31 39740.0 32 46288.0 33 52509.0 34 58846.5 35 64268.5 36 69508.5 37 75223.0 38 78277.5 39 79854.0 40 82042.5 41 83946.5 42 85368.0 43 86823.0 44 88759.0 45 92049.0 46 93035.5 47 92840.0 48 92905.5 49 91456.0 50 89847.5 51 87516.0 52 84511.5 53 81356.5 54 77776.0 55 72290.5 56 66083.5 57 59296.5 58 52473.5 59 46234.0 60 38402.5 61 30503.0 62 23993.0 63 18300.5 64 13741.5 65 10301.5 66 7846.5 67 5990.5 68 4512.0 69 3544.0 70 2794.5 71 2202.5 72 1755.5 73 1394.5 74 1076.5 75 843.5 76 640.0 77 469.0 78 341.0 79 253.5 80 195.5 81 140.0 82 108.0 83 83.5 84 62.0 85 45.0 86 36.5 87 33.5 88 25.0 89 20.5 90 14.0 91 4.0 92 1.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1233735047234769 2 0.0 3 0.003982359739298801 4 5.973539608948203E-4 5 2.787651817509161E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.01756220645030772 26-27 0.008203661062955532 28-29 0.006451422777664059 30-31 0.0024093276422757753 32-33 0.0012942669152721108 34-35 0.0024690630383652572 36-37 0.0015332084996300388 38-39 8.362955452527484E-4 40-41 0.0024491512396687632 42-43 0.001632767493112509 44-45 7.168247530737843E-4 46-47 0.03424829375796969 48-49 0.019254709339509707 50-51 0.08151890386344648 52-53 0.0016526792918090027 54-55 0.037016033776782364 56-57 0.0013739141100580867 58-59 0.0012942669152721108 60-61 0.0034845647718864513 62-63 0.0033053585836180055 64-65 0.010692635900017284 66-67 0.006232392992002626 68-69 0.0026084456292407156 70-71 0.005973539608948203 72-73 0.005216891258481431 74-75 0.0012146197204861346 76-77 0.0 78-79 0.0 80-81 2.787651817509161E-4 82-83 4.181477726263742E-4 84-85 0.0 86-87 0.0016725910905054967 88-89 0.0042412131223532245 90-91 0.0014734731035405567 92-93 0.003743418154940874 94-95 9.159427400387244E-4 96-97 5.973539608948203E-4 98-99 0.002110650661828365 100-101 0.0035642119666724277 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2511074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.5096041600693 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5618475752947 51.32503293326032 2 12.502014552804978 16.1300002000975 3 3.7264108429338036 7.211678652463495 4 1.535709986547026 3.96272173347256 5 0.7898263526525652 2.5475692682404145 6 0.46376431194476103 1.795035131227406 7 0.2847574465919056 1.2858713112893134 8 0.19976662848069687 1.0309492902145105 9 0.14859139765166543 0.8627015019690351 >10 0.7147894691680948 8.228668353121575 >50 0.04975615245631753 2.2266041201375057 >100 0.02177036368018217 2.3919301447365617 >500 6.840073579030118E-4 0.31104144582311716 >1k 1.8654746124199755E-4 0.23281977708630835 >5k 1.243649741613317E-4 0.45737613686045625 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6306 0.25112760516018245 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5096 0.20294105231466694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3366 0.1340462288247977 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 3.982359739298802E-5 0.0 0.0 0.0 10-11 0.0 3.982359739298802E-5 0.0 0.0 0.0 12-13 0.0 3.982359739298802E-5 0.0 0.0 1.991179869649401E-5 14-15 0.0 5.9735396089482024E-5 0.0 0.0 3.982359739298802E-5 16-17 0.0 7.964719478597604E-5 0.0 0.0 3.982359739298802E-5 18-19 0.0 7.964719478597604E-5 0.0 1.991179869649401E-5 5.9735396089482024E-5 20-21 0.0 7.964719478597604E-5 0.0 7.964719478597604E-5 7.964719478597604E-5 22-23 0.0 7.964719478597604E-5 0.0 1.7920618826844609E-4 9.955899348247005E-5 24-25 0.0 1.1947079217896405E-4 0.0 1.991179869649401E-4 1.5929438957195207E-4 26-27 0.0 1.5929438957195207E-4 0.0 5.376185648053383E-4 1.5929438957195207E-4 28-29 0.0 1.7920618826844609E-4 0.0 0.002250033252703823 1.7920618826844609E-4 30-31 0.0 2.3894158435792812E-4 0.0 0.00804436667338358 1.991179869649401E-4 32-33 0.0 2.3894158435792812E-4 0.0 0.023276892676201498 1.991179869649401E-4 34-35 0.0 2.9867698044741016E-4 0.0 0.04866443601423136 1.991179869649401E-4 36-37 3.982359739298802E-5 3.5841237653689217E-4 0.0 0.08707429569976831 2.3894158435792812E-4 38-39 3.982359739298802E-5 4.7788316871585625E-4 0.0 0.14017906282331782 2.5885338305442214E-4 40-41 3.982359739298802E-5 5.575303635018322E-4 0.0 0.19364224232340427 2.986769804474101E-4 42-43 3.982359739298802E-5 5.575303635018322E-4 0.0 0.24937536687489098 3.1858877914390415E-4 44-45 3.982359739298802E-5 5.774421621983262E-4 0.0 0.3215556371496818 3.1858877914390415E-4 46-47 5.9735396089482024E-5 5.973539608948203E-4 0.0 0.4054241332593146 3.982359739298802E-4 48-49 7.964719478597604E-5 5.973539608948203E-4 0.0 0.49431040264046383 3.982359739298802E-4 50-51 7.964719478597604E-5 6.770011556807964E-4 0.0 0.5828382596450762 3.982359739298802E-4 52-53 7.964719478597604E-5 6.770011556807964E-4 0.0 0.6738550914867503 3.982359739298802E-4 54-55 1.3938259087545806E-4 6.770011556807964E-4 0.0 0.772995937196594 3.982359739298802E-4 56-57 2.389415843579281E-4 7.168247530737843E-4 0.0 0.8919291108107528 4.380595713228682E-4 58-59 2.787651817509161E-4 7.168247530737843E-4 0.0 1.0186876213126335 4.7788316871585625E-4 60-61 2.787651817509161E-4 7.367365517702783E-4 0.0 1.148452813417685 4.7788316871585625E-4 62-63 2.787651817509161E-4 7.566483504667724E-4 0.0 1.277102944795733 5.575303635018322E-4 64-65 2.986769804474101E-4 7.566483504667724E-4 0.0 1.4110496146270481 5.575303635018322E-4 66-67 3.385005778403982E-4 8.362955452527485E-4 0.0 1.5681935299397787 5.575303635018322E-4 68-69 3.982359739298802E-4 8.362955452527485E-4 0.0 1.7451695967542173 5.575303635018322E-4 70-71 3.982359739298802E-4 8.362955452527485E-4 0.0 1.931364826365133 5.774421621983262E-4 72-73 3.982359739298802E-4 8.562073439492425E-4 0.0 2.131319108875326 5.973539608948203E-4 74-75 3.982359739298802E-4 8.761191426457364E-4 0.0 2.3381031383384165 5.973539608948203E-4 76-77 3.982359739298802E-4 9.358545387352184E-4 0.0 2.560736959563916 5.973539608948203E-4 78-79 3.982359739298802E-4 9.756781361282066E-4 0.0 2.804915346979022 5.973539608948203E-4 80-81 4.1814777262637424E-4 9.955899348247006E-4 0.0 3.0725299214599016 6.172657595913143E-4 82-83 4.380595713228682E-4 9.955899348247006E-4 0.0 3.345102533816208 6.371775582878083E-4 84-85 4.380595713228682E-4 9.955899348247006E-4 0.0 3.6159229078872226 6.570893569843024E-4 86-87 4.380595713228682E-4 9.955899348247006E-4 0.0 3.902951486097184 6.969129543772904E-4 88-89 4.380595713228682E-4 9.955899348247006E-4 0.0 4.217995965073111 7.566483504667723E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3700 0.0 52.44671 1 GTATCAA 6380 0.0 39.287083 1 ATCAACG 7805 0.0 31.640972 3 CAACGCA 7890 0.0 31.238667 5 TCAACGC 8040 0.0 30.773989 4 TATCAAC 8370 0.0 29.732077 2 AACGCAG 8335 0.0 29.456902 6 GTGGTAT 1630 0.0 29.179123 1 ACGCAGA 9510 0.0 25.218136 7 TGGTATC 1850 0.0 24.90115 2 CGCAGAG 9690 0.0 24.210585 8 GTACATG 7250 0.0 21.780102 1 GAGTACT 6505 0.0 21.427076 12-13 GCAGAGT 10920 0.0 21.00519 9 TACATGG 7470 0.0 20.090275 2 AGAGTAC 9940 0.0 19.516764 10-11 CAGAGTA 10795 0.0 19.290749 10-11 GTACTTT 7320 0.0 19.26848 14-15 ACATGGG 7345 0.0 18.880367 3 AGTACTT 7020 0.0 17.994783 12-13 >>END_MODULE