FastQCFastQC Report
Sat 18 Jun 2016
SRR1931137_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931137_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670369
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53510.20038429145934514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39100.14642171175594085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32350.047.1532941
GTATCAA59350.041.7051
CAACGCA67550.035.3355035
ATCAACG67900.035.3225673
TCAACGC69750.034.6662254
AACGCAG72250.033.4852266
TATCAAC74800.032.578382
CGTACCG650.005760209629.3187585
ACGCAGA82800.028.591387
CGCAGAG83150.027.8408898
GTGGTAT16400.023.3994311
GCAGAGT95550.022.8319959
GTACATG65350.022.4613061
TACATGG66200.021.0863972
AGAGTAC83600.020.7682710-11
GAGTACT55950.020.50175912-13
ACATGGG67500.020.0309243
TGGTATC17800.019.4034922
CAGAGTA93250.019.07878910-11
GTACTAG7850.018.3320391
Produced by FastQC (version 0.11.5)