##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931137_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2670369 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.457727377751915 31.0 28.0 31.0 22.0 34.0 2 28.156617680927244 31.0 28.0 33.0 16.0 34.0 3 28.224485829486486 31.0 28.0 33.0 16.0 34.0 4 31.38042045874559 35.0 31.0 37.0 17.0 37.0 5 31.46418378883218 35.0 32.0 37.0 17.0 37.0 6 31.738568340180702 35.0 32.0 37.0 18.0 37.0 7 31.375851052794577 35.0 31.0 37.0 17.0 37.0 8 31.572672915241302 35.0 32.0 37.0 17.0 37.0 9 32.54981390212364 37.0 32.0 39.0 17.0 39.0 10-11 32.53772156582106 37.0 31.5 39.0 17.0 39.0 12-13 32.568172600865275 37.0 32.0 39.0 17.0 39.0 14-15 33.311263724226876 38.0 31.5 40.0 17.0 41.0 16-17 33.299605972058544 38.0 31.0 40.0 17.0 41.0 18-19 33.300483753368916 38.0 31.0 40.0 17.5 41.0 20-21 33.55780343465641 38.0 32.0 40.0 18.0 41.0 22-23 33.431460408655134 38.0 32.0 40.0 18.0 41.0 24-25 33.33115629338118 38.0 31.5 40.0 17.5 41.0 26-27 32.968922272539864 37.0 31.0 40.0 16.5 41.0 28-29 32.910419309091736 37.0 31.0 40.0 16.5 41.0 30-31 32.642868083025235 37.0 30.5 40.0 15.5 41.0 32-33 32.43512095144903 37.0 30.0 40.0 15.5 41.0 34-35 32.40844785870417 37.0 30.0 40.0 15.0 41.0 36-37 32.05137529682227 36.0 30.0 40.0 9.5 41.0 38-39 31.88248833775407 36.0 30.0 40.0 9.0 41.0 40-41 31.723311085471707 36.0 30.0 40.0 9.0 41.0 42-43 31.957647800734655 37.0 30.0 40.0 9.0 41.0 44-45 32.00855368677512 37.0 30.0 40.0 8.5 41.0 46-47 31.799926152527984 36.5 30.0 40.0 8.0 41.0 48-49 31.570881215292715 36.0 30.0 40.0 6.5 41.0 50-51 31.030696132257376 35.5 29.0 39.5 2.0 40.5 52-53 30.97786129931856 35.5 29.0 39.0 2.0 40.5 54-55 30.975388981822363 36.0 29.0 40.0 2.0 41.0 56-57 30.71039957399146 35.0 28.5 40.0 2.0 41.0 58-59 30.54154182436959 35.0 28.0 39.0 2.0 41.0 60-61 30.388695157860205 35.0 28.0 39.0 2.0 41.0 62-63 30.22162311650562 35.0 28.0 39.0 2.0 40.0 64-65 29.869614648762024 34.0 28.0 39.0 2.0 40.0 66-67 29.63175276525454 34.0 28.0 38.0 2.0 40.0 68-69 29.43493614552895 34.0 28.0 38.0 2.0 40.0 70-71 29.13938298414938 34.0 28.0 37.5 2.0 40.0 72-73 28.799368551687053 34.0 27.5 37.0 2.0 40.0 74-75 28.37909180341743 34.0 26.0 36.5 2.0 39.0 76-77 28.08296531303352 33.0 26.0 36.0 2.0 39.0 78-79 27.68113901112543 33.0 26.0 35.5 2.0 38.5 80-81 27.28871684025691 33.0 26.0 35.0 2.0 37.0 82-83 26.965581910215406 33.0 25.5 35.0 2.0 37.0 84-85 26.639511243577196 32.5 25.0 35.0 2.0 36.5 86-87 26.328189662177774 32.0 25.0 35.0 2.0 36.0 88-89 25.988240576489616 32.0 24.0 35.0 2.0 36.0 90-91 25.60217595395992 32.0 22.5 35.0 2.0 35.0 92-93 25.245566811178527 32.0 20.0 35.0 2.0 35.0 94-95 24.946185527168716 32.0 18.5 35.0 2.0 35.0 96-97 24.54289819122376 32.0 12.0 34.0 2.0 35.0 98-99 24.173264443977594 31.5 2.0 34.0 2.0 35.0 100-101 23.57256749909844 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.183269823697984 1101 2 5.91114645358812 1101 3 5.410111460972409 1101 4 6.800413953591221 1101 5 6.2105311810966946 1101 6 5.87788662058508 1101 7 6.339518130747681 1101 8 5.817763541996829 1101 9 6.394231428756875 1101 10-11 6.470776470186436 1101 12-13 6.289376102729445 1101 14-15 6.572853566616693 1101 16-17 6.508073896873778 1101 18-19 6.534273988034478 1101 20-21 5.874235377274633 1101 22-23 5.901265959910582 1101 24-25 5.841843002388842 1101 26-27 6.141961717088307 1101 28-29 6.014325677508879 1101 30-31 5.96023875832687 1101 32-33 5.916179880065599 1101 34-35 5.840695536944814 1101 36-37 5.875763339599779 1101 38-39 5.7410366859964626 1101 40-41 5.941433865322136 1101 42-43 5.796767986576427 1101 44-45 5.735179250918989 1101 46-47 5.486199340067927 1101 48-49 5.4488474876377175 1101 50-51 5.3832727644794325 1101 52-53 5.249271298848672 1101 54-55 5.4039401109395655 1101 56-57 5.473479534956958 1101 58-59 5.225094932527419 1101 60-61 5.159880732681376 1101 62-63 5.098590475173211 1101 64-65 5.187178172327371 1101 66-67 5.162285927371663 1101 68-69 5.062998313580369 1101 70-71 4.83450665981081 1101 72-73 4.714538518230622 1101 74-75 4.92480310654955 1101 76-77 4.649734002956816 1101 78-79 4.722468542992036 1101 80-81 4.5294680380614984 1101 82-83 4.462394673031252 1101 84-85 4.394686698365181 1101 86-87 4.301801137015822 1101 88-89 4.333843757912611 1101 90-91 4.301426028572386 1101 92-93 4.231270651560681 1101 94-95 4.129824636333364 1101 96-97 4.219652942473534 1101 98-99 3.961478446941932 1101 100-101 3.9347253540763703 1102 1 5.453467495524137 1102 2 6.022193305000695 1102 3 5.743920577175267 1102 4 7.147753000090436 1102 5 6.631606563525306 1102 6 6.103372162080383 1102 7 6.6074353810824356 1102 8 6.191228290040062 1102 9 6.962209704046877 1102 10-11 6.936370986266667 1102 12-13 7.074238548534797 1102 14-15 7.253318589250476 1102 16-17 7.209603553304877 1102 18-19 7.235581636635764 1102 20-21 6.494783235446665 1102 22-23 6.554917861787331 1102 24-25 6.410744992713333 1102 26-27 6.575184648488104 1102 28-29 6.459585480340401 1102 30-31 6.664383044704056 1102 32-33 6.460949379946719 1102 34-35 6.384498675400103 1102 36-37 6.373614483676263 1102 38-39 6.309722398193372 1102 40-41 6.499622400376527 1102 42-43 6.47435706132525 1102 44-45 6.263655653466692 1102 46-47 6.214425274847088 1102 48-49 5.9042148478594605 1102 50-51 5.878285747694594 1102 52-53 5.673931516626684 1102 54-55 6.059870040887795 1102 56-57 5.958230403877174 1102 58-59 5.965256924990033 1102 60-61 5.824768725705933 1102 62-63 5.812255786003753 1102 64-65 5.818256788048718 1102 66-67 5.90812474925205 1102 68-69 5.834882331061905 1102 70-71 5.675170675728648 1102 72-73 5.489961605971512 1102 74-75 5.556368094210381 1102 76-77 5.412919824743351 1102 78-79 5.292606553387021 1102 80-81 5.357417844709435 1102 82-83 5.31672025734121 1102 84-85 5.257272627580008 1102 86-87 5.3056704992245365 1102 88-89 5.345411809150946 1102 90-91 5.3683324143763045 1102 92-93 5.352341653305555 1102 94-95 5.242636961314531 1102 96-97 5.221406303886763 1102 98-99 5.0651302835435885 1102 100-101 4.967303208037428 1103 1 5.0562016842044635 1103 2 5.707523843649351 1103 3 5.305931878861518 1103 4 6.756266255529244 1103 5 6.309534740671221 1103 6 5.6525224251735935 1103 7 6.253261375076978 1103 8 5.741723086116078 1103 9 6.2899500376839015 1103 10-11 6.4074530233505484 1103 12-13 6.474909304804466 1103 14-15 6.662063610480224 1103 16-17 6.559923057169929 1103 18-19 6.464808706121154 1103 20-21 5.751482593573389 1103 22-23 6.008511264983909 1103 24-25 5.7350429162843 1103 26-27 6.034193309900168 1103 28-29 5.817196571912206 1103 30-31 6.075526995070437 1103 32-33 5.813983797803196 1103 34-35 5.889173973371207 1103 36-37 5.905059187924024 1103 38-39 5.804926282483677 1103 40-41 5.898524890296525 1103 42-43 5.918434760684441 1103 44-45 5.588849835550448 1103 46-47 5.662003372914498 1103 48-49 5.524185385950499 1103 50-51 5.529804188518444 1103 52-53 5.157053796472898 1103 54-55 5.453925566125967 1103 56-57 5.501643131309557 1103 58-59 5.652175955516743 1103 60-61 5.342636173544474 1103 62-63 5.3754878018525645 1103 64-65 5.102620528244497 1103 66-67 5.296679437043231 1103 68-69 5.285775704663948 1103 70-71 5.197289809182781 1103 72-73 5.105193823376837 1103 74-75 5.146608271742753 1103 76-77 5.181889613435256 1103 78-79 5.160675567422306 1103 80-81 5.076877385724007 1103 82-83 5.001600778956309 1103 84-85 5.057314090911291 1103 86-87 5.113678527408371 1103 88-89 5.038704120602425 1103 90-91 5.019462747576718 1103 92-93 4.940936245801748 1103 94-95 4.87468917645646 1103 96-97 4.690373328679275 1103 98-99 4.5290903182360935 1103 100-101 4.569137701482436 1104 1 4.5836392567924165 1104 2 5.463226958272259 1104 3 5.071896737902033 1104 4 6.538997543140855 1104 5 5.979176822090739 1104 6 5.764442046527414 1104 7 6.213072146526496 1104 8 5.650509649527638 1104 9 6.3096022111036945 1104 10-11 6.2525765782682825 1104 12-13 6.162620959103119 1104 14-15 6.192875905419989 1104 16-17 6.108044787019143 1104 18-19 6.291765455164075 1104 20-21 5.667202053927898 1104 22-23 5.78903535886645 1104 24-25 5.571933667899614 1104 26-27 5.825263709519746 1104 28-29 5.753594588113295 1104 30-31 5.829606621189004 1104 32-33 5.709380002171166 1104 34-35 5.532692947513468 1104 36-37 5.642451254335526 1104 38-39 5.50225046069469 1104 40-41 5.751216405500468 1104 42-43 5.719867966532419 1104 44-45 5.460604832035969 1104 46-47 5.498822038813515 1104 48-49 5.330775225787857 1104 50-51 5.200363223883937 1104 52-53 5.005422579434384 1104 54-55 5.271875004022824 1104 56-57 5.247798693703402 1104 58-59 5.220119413844344 1104 60-61 5.112694154095035 1104 62-63 4.977509132606883 1104 64-65 5.047254042099372 1104 66-67 5.141138361656697 1104 68-69 4.996629757322918 1104 70-71 4.894039570590877 1104 72-73 4.7869588078701355 1104 74-75 4.720445340061577 1104 76-77 4.782357792436617 1104 78-79 4.82456459418005 1104 80-81 4.778662771656752 1104 82-83 4.529835406854566 1104 84-85 4.4581976018118326 1104 86-87 4.416715864973256 1104 88-89 4.488272811672353 1104 90-91 4.504793493387357 1104 92-93 4.457080751901142 1104 94-95 4.3531711647175975 1104 96-97 4.1141616623269535 1104 98-99 4.02774756223608 1104 100-101 4.100395627956896 1105 1 4.5997758920475995 1105 2 5.201908359376485 1105 3 4.951082550880265 1105 4 6.442364304033429 1105 5 3.7656515173515075 1105 6 4.97912095658225 1105 7 5.676043756593547 1105 8 1.2527876441474497 1105 9 0.8148972287486309 1105 10-11 2.316351030660279 1105 12-13 5.6773053364817265 1105 14-15 3.8701061250270143 1105 16-17 5.972946874938831 1105 18-19 6.362358172859665 1105 20-21 3.4587849381291242 1105 22-23 5.513165050620451 1105 24-25 5.739995527250997 1105 26-27 5.8379115087937805 1105 28-29 3.206650181399077 1105 30-31 5.547306654761787 1105 32-33 5.84145140883669 1105 34-35 5.712607964799609 1105 36-37 5.87869593757673 1105 38-39 5.869671447187841 1105 40-41 5.957204577287197 1105 42-43 3.1130075857503954 1105 44-45 5.268326658019603 1105 46-47 5.56574139835071 1105 48-49 5.435126617520748 1105 50-51 5.630216093075649 1105 52-53 5.432318566162454 1105 54-55 5.583965307126128 1105 56-57 5.650792907737163 1105 58-59 5.508469315731514 1105 60-61 5.542136034307678 1105 62-63 5.32922916120782 1105 64-65 5.378479294984455 1105 66-67 5.464508522541241 1105 68-69 5.4365003867741954 1105 70-71 5.194977131190608 1105 72-73 5.200139927492618 1105 74-75 5.253637206392344 1105 76-77 5.095062058913975 1105 78-79 5.077581745345572 1105 80-81 5.122578708136697 1105 82-83 4.960831630129817 1105 84-85 5.009092662511939 1105 86-87 4.908571602801413 1105 88-89 4.8728946821634125 1105 90-91 4.814030280617054 1105 92-93 4.8213382936044376 1105 94-95 4.800448821901018 1105 96-97 4.718247803430035 1105 98-99 4.576040433102165 1105 100-101 4.688876660187624 1106 1 4.423534305400821 1106 2 4.678220156449495 1106 3 4.772396446545066 1106 4 6.311002330617949 1106 5 6.161987284950062 1106 6 5.329526739177254 1106 7 6.071665395429189 1106 8 5.542421663915224 1106 9 6.201120264755303 1106 10-11 6.152817917210761 1106 12-13 6.3362866385098435 1106 14-15 6.1117328323795235 1106 16-17 6.183930281634279 1106 18-19 6.248743349764119 1106 20-21 5.600878177119487 1106 22-23 5.650259388270449 1106 24-25 5.749250532227876 1106 26-27 5.9625423882682895 1106 28-29 5.9393712588044565 1106 30-31 5.962943517880948 1106 32-33 5.833534857352085 1106 34-35 5.5092776464515865 1106 36-37 5.6062765093252835 1106 38-39 5.680057286832138 1106 40-41 5.764004172909569 1106 42-43 2.823500994045734 1106 44-45 4.879098794239226 1106 46-47 5.184336200019857 1106 48-49 5.076817985043153 1106 50-51 5.278071979056055 1106 52-53 5.037303215305247 1106 54-55 5.288138827758154 1106 56-57 5.291627244799869 1106 58-59 5.237505643140185 1106 60-61 5.012835142570715 1106 62-63 5.1555487299054406 1106 64-65 5.106661439841464 1106 66-67 5.362393140749425 1106 68-69 5.286690266296294 1106 70-71 5.147597086343016 1106 72-73 4.842437393132922 1106 74-75 5.0070995664144995 1106 76-77 5.012374346333463 1106 78-79 5.12587090572659 1106 80-81 5.146303807294526 1106 82-83 5.096026286543001 1106 84-85 5.113685724299142 1106 86-87 5.109376079864742 1106 88-89 5.249551784922012 1106 90-91 5.3953262521815155 1106 92-93 5.319842160936982 1106 94-95 5.117059302506387 1106 96-97 5.0410912472166345 1106 98-99 4.972437240863059 1106 100-101 5.029590094060971 1107 1 3.015167623857259 1107 2 0.2014623737859722 1107 3 1.0700322181916988 1107 4 2.734692090420978 1107 5 2.767860488864379 1107 6 4.1475520333953355 1107 7 5.1304719438594795 1107 8 4.541686305926181 1107 9 4.897345152504627 1107 10-11 2.736067724010802 1107 12-13 5.0938905171862565 1107 14-15 3.229240904842989 1107 16-17 2.013790009476935 1107 18-19 2.4754994475442658 1107 20-21 1.9411842754987099 1107 22-23 4.270098122466244 1107 24-25 4.3818645202045445 1107 26-27 3.56415143317156 1107 28-29 4.341668147985565 1107 30-31 3.575299778608038 1107 32-33 4.235740107598453 1107 34-35 4.89634197305238 1107 36-37 4.992989256928254 1107 38-39 5.079151306319872 1107 40-41 3.565967380298492 1107 42-43 4.1810969912121365 1107 44-45 4.6532434538328715 1107 46-47 4.863737901677183 1107 48-49 2.2723353153791415 1107 50-51 3.9449249501419494 1107 52-53 4.1003571675174655 1107 54-55 4.61644275301223 1107 56-57 3.250180705026139 1107 58-59 2.288528123977443 1107 60-61 3.288440783260725 1107 62-63 4.206738669429356 1107 64-65 4.312023169325574 1107 66-67 4.511824043307751 1107 68-69 4.5491034573605695 1107 70-71 4.36516748077047 1107 72-73 4.225091163078197 1107 74-75 4.4141971563573215 1107 76-77 4.30958112795161 1107 78-79 4.377994139594396 1107 80-81 4.368983610560054 1107 82-83 4.299638358245648 1107 84-85 4.267828496823409 1107 86-87 4.283183781504093 1107 88-89 4.283860129063555 1107 90-91 4.2408169239183096 1107 92-93 4.207706526327531 1107 94-95 4.243397661534818 1107 96-97 4.406440141633606 1107 98-99 4.337635244613232 1107 100-101 4.528281969328681 1108 1 -2.0605335930181568 1108 2 -1.2180368058848394 1108 3 -1.8876585454566808 1108 4 -3.4453333133216866 1108 5 -8.501027542332654 1108 6 -6.654828405722817 1108 7 -5.489971098445238 1108 8 -5.5924719175673765 1108 9 -7.3567519109621315 1108 10-11 -3.1676072991275177 1108 12-13 -1.7459176509947802 1108 14-15 -6.2931683458369605 1108 16-17 -5.705144233812955 1108 18-19 -3.911906853564954 1108 20-21 -4.460806458847589 1108 22-23 -3.4139233364675974 1108 24-25 -7.998623908055052 1108 26-27 -8.74425271352473 1108 28-29 -5.951971235044276 1108 30-31 -5.721501647512639 1108 32-33 -2.4762427655287027 1108 34-35 -0.9660173091886257 1108 36-37 -4.3678843968863745 1108 38-39 -5.36421874282243 1108 40-41 -2.936961278815037 1108 42-43 -3.6469036645348822 1108 44-45 -1.380249083172302 1108 46-47 -2.0931284020911676 1108 48-49 -1.2042257949502932 1108 50-51 -0.6378196092877033 1108 52-53 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-5.4931280080145015 2313 56-57 -3.7437643552719635 2313 58-59 -4.309839355512953 2313 60-61 -3.060801721037077 2313 62-63 -4.33327792897936 2313 64-65 -4.230892995823439 2313 66-67 -5.486244845243213 2313 68-69 -5.036828746902952 2313 70-71 -5.068391846942429 2313 72-73 -5.88533658100733 2313 74-75 -7.222938819147142 2313 76-77 -5.951319309947404 2313 78-79 -7.096829888921878 2313 80-81 -8.713297072655187 2313 82-83 -7.949876447586476 2313 84-85 -8.656929679310448 2313 86-87 -7.164766418114967 2313 88-89 -8.260628245606858 2313 90-91 -8.668585146758954 2313 92-93 -7.596520526501287 2313 94-95 -7.891914164315917 2313 96-97 -8.054982089409028 2313 98-99 -7.997850426372285 2313 100-101 -7.53044907717006 2314 1 -13.427217251275591 2314 2 -14.6397058930517 2314 3 -13.739052657389587 2314 4 -24.836681067065445 2314 5 -19.05914166811531 2314 6 -18.337259421467287 2314 7 -17.98812157982021 2314 8 -13.814531490725793 2314 9 -15.166592927379856 2314 10-11 -13.987524970061315 2314 12-13 -15.159454819298091 2314 14-15 -16.818190226517522 2314 16-17 -17.211281404039838 2314 18-19 -15.90171863162272 2314 20-21 -17.246865339518532 2314 22-23 -16.302194329898583 2314 24-25 -16.9556714107721 2314 26-27 -16.178576047467196 2314 28-29 -17.393509903737108 2314 30-31 -18.157344606742438 2314 32-33 -15.507447020479507 2314 34-35 -16.148112660866467 2314 36-37 -15.87189793886362 2314 38-39 -15.424308381478621 2314 40-41 -17.376574447618474 2314 42-43 -17.983149613002272 2314 44-45 -14.989608243723598 2314 46-47 -13.806129854391283 2314 48-49 -13.726526776936003 2314 50-51 -13.512805422912393 2314 52-53 -13.139206593429993 2314 54-55 -13.391482241494423 2314 56-57 -12.027510250457567 2314 58-59 -11.110475001625144 2314 60-61 -11.555818332332759 2314 62-63 -14.345309115966858 2314 64-65 -12.511431854733047 2314 66-67 -13.211534342679503 2314 68-69 -16.07573566603571 2314 70-71 -16.029379306854473 2314 72-73 -14.318977815428239 2314 74-75 -12.559956083372363 2314 76-77 -13.198309112724274 2314 78-79 -12.929891168279703 2314 80-81 -13.098448686735422 2314 82-83 -13.264799728365382 2314 84-85 -13.374182382246712 2314 86-87 -13.82339949091661 2314 88-89 -13.059487539290725 2314 90-91 -12.654988849253527 2314 92-93 -12.749939764878182 2314 94-95 -12.541310204490289 2314 96-97 -12.433729232842285 2314 98-99 -12.58978866691921 2314 100-101 -12.128657361693708 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100415.0 3 16100.0 4 10932.0 5 8957.0 6 9249.0 7 10548.0 8 12257.0 9 14089.0 10 15212.0 11 16448.0 12 18166.0 13 19313.0 14 21227.0 15 23918.0 16 25595.0 17 26984.0 18 24867.0 19 23064.0 20 23207.0 21 25223.0 22 29673.0 23 36198.0 24 43873.0 25 52684.0 26 61997.0 27 70725.0 28 78187.0 29 87183.0 30 97324.0 31 108598.0 32 124122.0 33 146814.0 34 176655.0 35 212669.0 36 259339.0 37 306477.0 38 265513.0 39 66495.0 40 72.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.458019990979935 19.706256058951066 14.192179813934953 23.643544136134047 2 14.849043935521363 20.15771725617864 40.80961274075578 24.18362606754421 3 18.14940182424227 25.261190494646996 30.136621568030485 26.45278611308025 4 11.61718730884116 17.188814584631253 39.350384623347686 31.843613483179904 5 12.836813332035 37.23294705391608 35.433200529810485 14.49703908423844 6 30.387199234754785 37.33449079670438 18.483468102101163 13.794841866439672 7 26.67654806366229 32.0206369752429 22.64807944403272 18.65473551706209 8 25.00523887587032 35.40377956309837 20.914567148893163 18.676414412138147 9 25.168148028745048 16.286385838903282 21.351969380601968 37.193496751749706 10-11 23.886263265088367 26.45642423979893 29.127247149240134 20.53006534587257 12-13 24.863853549234687 24.051538098096053 28.793246685350343 22.291361667318917 14-15 22.05244296358589 25.622637639956036 26.60360228799895 25.721317108459118 16-17 21.543454589385654 28.68288008601636 27.30931380919881 22.464351515399176 18-19 21.42721704697818 27.76645702911588 29.19827281114184 21.608053112764097 20-21 22.302053899034206 27.043144532073722 29.116293890786743 21.538507678105333 22-23 22.094353225910133 26.827082431023552 28.894478342035175 22.18408600103114 24-25 21.92097601895724 27.052654542019773 28.915000155553116 22.111369283469877 26-27 21.70513950392893 27.155135630981853 28.992731800571548 22.146993064517673 28-29 21.73342005525711 27.18291828020678 29.015025135612344 22.06863652892376 30-31 21.935143063023613 26.98836945119496 28.979738205801702 22.096749279979726 32-33 21.822656488526725 27.20870653419266 28.8079982202187 22.160638757061914 34-35 22.130492082244388 27.227914628222006 28.8039847612805 21.83760852825311 36-37 22.015262355362815 26.956132586720443 28.79586079433017 22.232744263586575 38-39 21.980811863461458 27.175144473451102 28.871836933647664 21.972206729439776 40-41 21.993607389981637 26.9868007391631 28.849497012589726 22.170094858265532 42-43 21.922983205877944 27.125805967911234 28.719054580896685 22.23215624531414 44-45 21.979171996235987 27.26655455595481 28.5877629902337 22.1665104575755 46-47 22.078300055509892 27.219880262054662 28.398762665419287 22.30305701701616 48-49 22.033122278730012 27.366605294406927 28.420085371412124 22.180187055450936 50-51 21.907948624109025 27.489841997454107 28.265903460232707 22.336305918204165 52-53 21.951883663819082 27.63179199937631 28.127633066366016 22.288691270438594 54-55 21.96006012164041 27.516579646526218 28.06361972236181 22.459740509471562 56-57 21.92026402982431 27.558901756436168 28.002307180714947 22.518527033024576 58-59 21.92842180876353 27.610762332175508 28.131210090818193 22.329605768242768 60-61 22.054745017643803 27.65481717936557 27.95233425729592 22.338103545694704 62-63 21.924385730960775 27.594763120752226 28.028935326915494 22.451915821371504 64-65 21.861416905304097 27.55658487647213 28.072468636356994 22.509529581866776 66-67 21.91185937224406 27.474405222649008 27.98000950430446 22.633725900802474 68-69 21.989713781129126 27.425741536094822 27.991843074870925 22.592701607905123 70-71 22.00849395720217 27.282184596960196 28.01120369506986 22.69811775076778 72-73 22.05659592363452 27.39982751447459 27.932581602018296 22.61099495987259 74-75 21.913057708503956 27.599425397763383 27.899608630867117 22.587908262865543 76-77 22.022330996203145 27.4999073161799 27.83270776435766 22.645053923259294 78-79 22.036299103232547 27.542710389463025 27.742738924845217 22.678251582459204 80-81 22.02935249772597 27.61024787211056 27.73669107153356 22.62370855862991 82-83 22.15499056497435 27.439372611051134 27.86073759843677 22.544899225537744 84-85 22.192120265027043 27.533254767412295 27.68072502339564 22.59389994416502 86-87 22.051465546521847 27.460998835741428 27.786478198331395 22.70105741940533 88-89 22.184237459317423 27.384305315108136 27.75756833606142 22.673888889513023 90-91 22.202792947341734 27.311731075368233 27.792320087598384 22.69315588969165 92-93 22.22088033526453 27.347231787067628 27.763971945450233 22.667915932217607 94-95 22.06065903251573 27.28448764945968 27.831471980089646 22.823381337934944 96-97 22.084438517673025 27.167144316010262 27.890190456824506 22.858226709492207 98-99 22.113367103947056 27.22599386077355 27.673815865897183 22.98682316938221 100-101 22.164708323081943 27.16326844716966 27.65967924283124 23.012343986917163 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 326.0 1 226.5 2 163.0 3 199.0 4 174.0 5 180.5 6 299.5 7 551.5 8 928.5 9 1245.0 10 1282.0 11 1227.5 12 1313.0 13 1494.0 14 1676.5 15 1867.0 16 2051.0 17 2293.0 18 2586.5 19 2879.5 20 3392.0 21 4038.0 22 4880.5 23 6230.5 24 8068.0 25 10533.5 26 13917.5 27 18141.5 28 23170.5 29 29112.5 30 35607.5 31 42383.0 32 49797.0 33 56932.0 34 63428.0 35 69000.0 36 75068.5 37 81436.0 38 84995.0 39 87392.5 40 89153.0 41 90693.0 42 92317.0 43 93573.5 44 95487.0 45 97653.5 46 98120.5 47 97323.0 48 97435.0 49 96667.5 50 94388.0 51 91896.5 52 88831.0 53 85125.5 54 80852.0 55 75018.5 56 68641.5 57 61826.5 58 54717.0 59 47914.5 60 40300.0 61 32901.0 62 26340.5 63 20465.0 64 15328.0 65 11222.0 66 8455.5 67 6402.5 68 4808.5 69 3731.0 70 2959.0 71 2256.5 72 1672.0 73 1338.0 74 1028.0 75 768.5 76 568.0 77 403.0 78 330.5 79 252.0 80 181.5 81 143.5 82 114.5 83 92.0 84 71.5 85 62.0 86 53.0 87 34.5 88 27.5 89 25.5 90 18.5 91 15.5 92 13.5 93 9.5 94 5.5 95 3.5 96 3.0 97 3.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02864772621311886 2 0.4424107679500473 3 0.0 4 0.5195536646807988 5 0.3950764856841882 6 0.0923467880281714 7 0.10223306217230653 8 0.1053037988382879 9 0.10406801457027101 10-11 0.1504848206371479 12-13 0.29190722330883856 14-15 0.11786760556312628 16-17 0.11120186011745943 18-19 0.1654640238858375 20-21 0.1079064354027477 22-23 0.0916539998779195 24-25 0.09249658006065828 26-27 0.16628788006451545 28-29 0.10631489505757445 30-31 0.11545220903927511 32-33 0.09831974532358637 34-35 0.1351685853153628 36-37 0.13033779226766037 38-39 0.1256942392605666 40-41 0.10326288239565394 42-43 0.10369353448905377 44-45 0.09251530406471914 46-47 0.08897646729721623 48-49 0.09350767627994483 50-51 0.08060683748201092 52-53 0.08901391530533795 54-55 0.2908586790814303 56-57 0.3087775509676753 58-59 0.3228954500295652 60-61 0.15486623758738963 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2670369.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.05985982154056 #Duplication Level Percentage of deduplicated Percentage of total 1 84.84274912826623 61.985993581852625 2 9.763263127846992 14.266052710426333 3 2.559410072604704 5.609704233910159 4 1.040266496203567 3.0400689755871104 5 0.5105462965765273 1.8650220430143873 6 0.3037028206666967 1.3313091303189195 7 0.1924134163449532 0.98403880581622 8 0.13806075431373302 0.8069359485613986 9 0.10660587941838527 0.7009749545813354 >10 0.5094838719084414 6.602478819554412 >50 0.024249363134776252 1.1927091894881099 >100 0.008934946802243505 1.0972249047185447 >500 1.569129563474288E-4 0.07871973168505933 >1k 1.0460863756387614E-4 0.23428673541590708 >5k 5.230431878193807E-5 0.20448023506940446 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5351 0.20038429145934514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3910 0.14642171175594085 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.7448008121724E-5 0.0 0.0 0.0 3 0.0 3.7448008121724E-5 0.0 0.0 0.0 4 0.0 3.7448008121724E-5 0.0 0.0 0.0 5 0.0 3.7448008121724E-5 0.0 0.0 0.0 6 0.0 3.7448008121724E-5 0.0 0.0 0.0 7 0.0 3.7448008121724E-5 0.0 0.0 3.7448008121724E-5 8 0.0 3.7448008121724E-5 0.0 0.0 7.4896016243448E-5 9 0.0 1.49792032486896E-4 0.0 3.7448008121724E-5 7.4896016243448E-5 10-11 0.0 1.49792032486896E-4 0.0 3.7448008121724E-5 7.4896016243448E-5 12-13 0.0 1.49792032486896E-4 0.0 7.4896016243448E-5 7.4896016243448E-5 14-15 0.0 1.8724004060862E-4 0.0 7.4896016243448E-5 1.1234402436517201E-4 16-17 0.0 1.8724004060862E-4 0.0 9.362002030431E-5 1.1234402436517201E-4 18-19 0.0 2.0596404466948203E-4 0.0 1.49792032486896E-4 2.05964044669482E-4 20-21 0.0 2.2468804873034402E-4 0.0 1.49792032486896E-4 2.99584064973792E-4 22-23 0.0 2.2468804873034402E-4 0.0 2.8086006091293005E-4 2.99584064973792E-4 24-25 0.0 2.2468804873034402E-4 0.0 4.1192808933896406E-4 2.99584064973792E-4 26-27 0.0 2.2468804873034402E-4 0.0 8.613041867996519E-4 2.99584064973792E-4 28-29 0.0 2.2468804873034402E-4 0.0 0.00243412052791206 3.37032073095516E-4 30-31 0.0 3.37032073095516E-4 0.0 0.00777046168525773 3.7448008121724E-4 32-33 0.0 3.37032073095516E-4 0.0 0.021364088633443544 4.30652093399826E-4 34-35 0.0 3.37032073095516E-4 0.0 0.0437579974902345 4.4937609746068804E-4 36-37 0.0 3.37032073095516E-4 0.0 0.07869698906780298 4.4937609746068804E-4 38-39 0.0 3.37032073095516E-4 0.0 0.12631213139457506 4.4937609746068804E-4 40-41 0.0 3.7448008121724E-4 0.0 0.17460133786753818 4.4937609746068804E-4 42-43 0.0 3.9320408527810204E-4 0.0 0.22549318090496107 4.4937609746068804E-4 44-45 0.0 4.6810010152155E-4 0.0 0.2888552106469181 4.4937609746068804E-4 46-47 0.0 5.242721137041361E-4 0.0 0.3680202998162426 4.4937609746068804E-4 48-49 0.0 5.242721137041361E-4 0.0 0.448290105225158 4.4937609746068804E-4 50-51 0.0 5.99168129947584E-4 0.0 0.5306008270767074 4.86824105582412E-4 52-53 0.0 5.99168129947584E-4 0.0 0.6148962933587081 6.17892134008446E-4 54-55 0.0 5.99168129947584E-4 0.0 0.7098644419554002 7.676841664953421E-4 56-57 0.0 6.36616138069308E-4 0.0 0.8223208103449373 7.86408170556204E-4 58-59 0.0 6.36616138069308E-4 0.0 0.94380214869181 7.86408170556204E-4 60-61 0.0 6.74064146191032E-4 0.0 1.0698334200254722 7.86408170556204E-4 62-63 0.0 7.11512154312756E-4 0.0 1.1973438876799425 8.23856178677928E-4 64-65 0.0 8.23856178677928E-4 0.0 1.3354146936247386 8.23856178677928E-4 66-67 0.0 8.23856178677928E-4 0.0 1.4917226795248149 8.23856178677928E-4 68-69 0.0 8.23856178677928E-4 0.0 1.6706118143222903 8.4258018273879E-4 70-71 0.0 8.23856178677928E-4 0.0 1.8635252281613515 8.61304186799652E-4 72-73 0.0 8.23856178677928E-4 0.0 2.0568318460856907 8.61304186799652E-4 74-75 0.0 8.23856178677928E-4 0.0 2.2518049003714466 8.61304186799652E-4 76-77 0.0 8.23856178677928E-4 0.0 2.4701829597332803 8.61304186799652E-4 78-79 0.0 8.23856178677928E-4 0.0 2.706835647058515 8.61304186799652E-4 80-81 0.0 8.23856178677928E-4 0.0 2.967661023626323 8.61304186799652E-4 82-83 0.0 8.23856178677928E-4 0.0 3.2399829386874996 8.61304186799652E-4 84-85 0.0 8.23856178677928E-4 0.0 3.513915118097911 8.61304186799652E-4 86-87 0.0 8.23856178677928E-4 0.0 3.800017900147882 8.61304186799652E-4 88-89 0.0 8.61304186799652E-4 0.0 4.10799031894094 8.800281908605141E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3235 0.0 47.153294 1 GTATCAA 5935 0.0 41.705 1 CAACGCA 6755 0.0 35.335503 5 ATCAACG 6790 0.0 35.322567 3 TCAACGC 6975 0.0 34.666225 4 AACGCAG 7225 0.0 33.485226 6 TATCAAC 7480 0.0 32.57838 2 CGTACCG 65 0.0057602096 29.318758 5 ACGCAGA 8280 0.0 28.59138 7 CGCAGAG 8315 0.0 27.840889 8 GTGGTAT 1640 0.0 23.399431 1 GCAGAGT 9555 0.0 22.831995 9 GTACATG 6535 0.0 22.461306 1 TACATGG 6620 0.0 21.086397 2 AGAGTAC 8360 0.0 20.76827 10-11 GAGTACT 5595 0.0 20.501759 12-13 ACATGGG 6750 0.0 20.030924 3 TGGTATC 1780 0.0 19.403492 2 CAGAGTA 9325 0.0 19.078789 10-11 GTACTAG 785 0.0 18.332039 1 >>END_MODULE