FastQCFastQC Report
Sat 18 Jun 2016
SRR1931137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670369
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70520.26408335327439764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56400.2112067658065234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36540.1368350216767795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35950.052.654411
GTATCAA68100.042.1134451
ATCAACG81350.034.3853573
CAACGCA83200.033.618895
TCAACGC83550.033.4780544
TATCAAC86900.032.954392
AACGCAG88550.031.5877086
ACGCAGA100600.027.379247
CGCAGAG101800.026.543368
GTGGTAT17850.024.2467611
GCAGAGT113500.022.8030019
TGGTATC18150.022.7645172
GAGTACT68850.022.14075712-13
GTACATG70550.020.9659211
AGAGTAC102350.020.39212210-11
TACATGG72450.020.1240672
CAGAGTA111600.019.97849710-11
GTACTTT76750.019.67615114-15
ACATGGG73100.018.9706083
AGTACTT75350.018.49763712-13
Produced by FastQC (version 0.11.5)