FastQCFastQC Report
Sat 18 Jun 2016
SRR1931136_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931136_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380459
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58510.2457929332116201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.18059542298355066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25980.10913861570394617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.056.1928441
GTATCAA58600.043.0731241
ATCAACG68800.035.1166043
CAACGCA69200.035.114225
TCAACGC70650.034.5423284
AACGCAG72450.033.9171376
TATCAAC74200.033.24492
ACGCAGA82500.028.8102537
CGCAGAG82750.028.3210878
GTACATG66400.023.5423451
GCAGAGT94200.023.3647489
ACATGGG62900.022.6523653
TACATGG67250.022.3211522
GAGTACT56450.021.60117312-13
AGAGTAC84400.020.50062610-11
CAGAGTA92550.020.39023610-11
GTACTTT63100.019.70177514-15
GTACTAG7450.019.250191
GTATAGG9150.018.2859291
AGTACTT64600.017.95606612-13
Produced by FastQC (version 0.11.5)