##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931136_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2380459 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.736494516393687 31.0 28.0 31.0 23.0 34.0 2 28.43672417798416 31.0 28.0 33.0 16.0 34.0 3 28.455751180759677 31.0 28.0 34.0 16.0 34.0 4 31.707422392068086 35.0 32.0 37.0 19.0 37.0 5 31.781235467613598 35.0 32.0 37.0 19.0 37.0 6 32.039725111837676 35.0 32.0 37.0 22.0 37.0 7 31.715946378408535 35.0 32.0 37.0 17.0 37.0 8 31.881482100720913 35.0 32.0 37.0 19.0 37.0 9 32.83359175688386 37.0 32.0 39.0 17.0 39.0 10-11 32.86642282013679 37.0 32.0 39.0 18.0 39.0 12-13 32.88629839875419 37.0 32.0 39.0 18.0 39.0 14-15 33.628885647683916 38.0 32.0 40.0 17.0 41.0 16-17 33.63099721524294 38.0 32.0 40.0 17.5 41.0 18-19 33.680167774366204 38.0 32.0 40.0 17.5 41.0 20-21 33.87470819703259 38.0 32.0 40.0 19.5 41.0 22-23 33.77729169038408 38.0 32.0 40.0 19.0 41.0 24-25 33.65809619069263 38.0 32.0 40.0 19.5 41.0 26-27 33.28683690834414 38.0 31.0 40.0 17.5 41.0 28-29 33.2473585976486 37.0 31.0 40.0 17.5 41.0 30-31 32.96410713227995 37.0 31.0 40.0 16.5 41.0 32-33 32.79323672451405 37.0 30.0 40.0 16.0 41.0 34-35 32.7919411760505 37.0 30.0 40.0 16.0 41.0 36-37 32.433032032897856 37.0 30.0 40.0 15.0 41.0 38-39 32.2394987689349 36.5 30.0 40.0 14.0 41.0 40-41 32.09933861494779 36.5 30.0 40.0 9.0 41.0 42-43 32.33571403666268 37.0 30.0 40.0 9.0 41.0 44-45 32.36507728131423 37.0 30.0 40.0 10.5 41.0 46-47 32.10534417942086 37.0 30.0 40.0 8.5 41.0 48-49 31.867778231005026 37.0 30.0 40.0 7.5 41.0 50-51 31.356756407062672 35.5 29.5 39.5 6.5 40.5 52-53 31.298600816061104 36.0 29.5 39.5 2.0 40.5 54-55 31.263361183704486 36.0 29.5 40.0 2.0 41.0 56-57 31.01340791838885 36.0 29.0 40.0 2.0 41.0 58-59 30.856058222384842 35.5 29.0 40.0 2.0 41.0 60-61 30.690477550758068 35.0 29.0 39.0 2.0 41.0 62-63 30.574194304543788 35.0 29.0 39.0 2.0 41.0 64-65 30.210203158298462 35.0 28.0 39.0 2.0 40.0 66-67 29.979539450164864 35.0 28.0 39.0 2.0 40.0 68-69 29.77071984856702 34.0 28.0 38.5 2.0 40.0 70-71 29.47357526426626 34.0 28.0 38.0 2.0 40.0 72-73 29.127528346423944 34.0 28.0 37.0 2.0 40.0 74-75 28.70871331957408 34.0 27.5 37.0 2.0 39.0 76-77 28.399768069939455 34.0 27.0 36.0 2.0 39.0 78-79 27.977238423346087 33.5 26.0 36.0 2.0 39.0 80-81 27.563444697010116 33.0 26.0 35.5 2.0 37.5 82-83 27.224602692169874 33.0 26.0 35.0 2.0 37.0 84-85 26.886994693040293 33.0 26.0 35.0 2.0 36.5 86-87 26.563986609305182 33.0 25.0 35.0 2.0 36.0 88-89 26.20650114116647 32.0 24.5 35.0 2.0 36.0 90-91 25.832292007549803 32.0 23.5 35.0 2.0 35.5 92-93 25.4748582101183 32.0 21.0 35.0 2.0 35.0 94-95 25.163763585090102 32.0 19.5 35.0 2.0 35.0 96-97 24.767177464514198 32.0 17.5 35.0 2.0 35.0 98-99 24.408252148010114 32.0 4.5 34.0 2.0 35.0 100-101 23.81213455052156 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.050355925857982 1101 2 5.617203940871029 1101 3 5.178957165312383 1101 4 6.577813404551758 1101 5 5.998813694124042 1101 6 5.718268559897876 1101 7 6.070785100905859 1101 8 5.516465350334297 1101 9 6.262726213399418 1101 10-11 6.278779458913576 1101 12-13 6.171674100127088 1101 14-15 6.359930612223497 1101 16-17 6.425360389463126 1101 18-19 6.350481087938615 1101 20-21 5.73819765287228 1101 22-23 5.626065607490698 1101 24-25 5.534526876748757 1101 26-27 5.708545935450587 1101 28-29 5.765778172494112 1101 30-31 6.002495545506484 1101 32-33 5.655383601816165 1101 34-35 5.452605203546909 1101 36-37 5.631097085440302 1101 38-39 5.4823206215369495 1101 40-41 5.7282874713155 1101 42-43 5.46907344032714 1101 44-45 5.393631648831796 1101 46-47 5.268923275449591 1101 48-49 5.130309980173887 1101 50-51 5.059790962562854 1101 52-53 4.856431116784087 1101 54-55 5.1686560956455345 1101 56-57 5.086434105227507 1101 58-59 4.775055094413901 1101 60-61 4.829889614193625 1101 62-63 4.819110420555653 1101 64-65 4.954601606067634 1101 66-67 4.839762345752831 1101 68-69 5.0060127520602435 1101 70-71 4.75425146951131 1101 72-73 4.644635347942369 1101 74-75 4.771727325399876 1101 76-77 4.620996531950411 1101 78-79 4.535687540573441 1101 80-81 4.414822101184395 1101 82-83 4.315320999391467 1101 84-85 4.197561652094066 1101 86-87 3.996662852028159 1101 88-89 4.218837983847607 1101 90-91 4.064246255472749 1101 92-93 3.8813390196112643 1101 94-95 3.815846246927908 1101 96-97 3.84613691182388 1101 98-99 3.543320664535102 1101 100-101 3.5666326370727006 1102 1 5.25143350204586 1102 2 5.891369477028309 1102 3 5.459039597407859 1102 4 6.962661620460036 1102 5 6.494857228244296 1102 6 5.9931979823026715 1102 7 6.4868293534806725 1102 8 6.050846887742541 1102 9 6.759049099558183 1102 10-11 6.783110683371461 1102 12-13 6.935829398306861 1102 14-15 7.148405069050721 1102 16-17 6.976862329290444 1102 18-19 7.067160961490611 1102 20-21 6.382749231570521 1102 22-23 6.373556404933002 1102 24-25 6.245967156269842 1102 26-27 6.431482967134436 1102 28-29 6.298355438056053 1102 30-31 6.569458733162591 1102 32-33 6.354865205153171 1102 34-35 6.402988414531663 1102 36-37 6.530545354598743 1102 38-39 6.268866873105928 1102 40-41 6.5508431938060845 1102 42-43 6.408493562971561 1102 44-45 6.324815746473998 1102 46-47 6.370414755423727 1102 48-49 6.146805908226998 1102 50-51 6.049492444692358 1102 52-53 5.857104139660528 1102 54-55 6.1506626124510255 1102 56-57 6.134926248715569 1102 58-59 6.0515665431986605 1102 60-61 5.890159605191812 1102 62-63 5.85987220426702 1102 64-65 5.956187330375485 1102 66-67 5.941199202049241 1102 68-69 5.933152056173604 1102 70-71 5.79378443178399 1102 72-73 5.630797490458395 1102 74-75 5.752147853759816 1102 76-77 5.64854244964933 1102 78-79 5.558892936167201 1102 80-81 5.565511289288477 1102 82-83 5.398142909297263 1102 84-85 5.432210232723683 1102 86-87 5.482969002421829 1102 88-89 5.459645410554678 1102 90-91 5.383516844478702 1102 92-93 5.390982317486749 1102 94-95 5.278229181096339 1102 96-97 5.252119789361185 1102 98-99 4.927776530293983 1102 100-101 4.768360995742125 1103 1 4.773331193213323 1103 2 5.437169663901059 1103 3 5.050884728615877 1103 4 6.5659912707052435 1103 5 6.038752389753835 1103 6 5.432583572846678 1103 7 5.973587985418639 1103 8 5.433887572807588 1103 9 5.909664164614526 1103 10-11 6.0553634114417605 1103 12-13 6.177648012346413 1103 14-15 6.236559490115642 1103 16-17 6.164233947551946 1103 18-19 5.9705124650806916 1103 20-21 5.294521788353244 1103 22-23 5.538886393105546 1103 24-25 5.437303477648335 1103 26-27 5.460809542987128 1103 28-29 5.455425661381245 1103 30-31 5.8383876474297836 1103 32-33 5.447921864480318 1103 34-35 5.439193970291534 1103 36-37 5.516112632785955 1103 38-39 5.296176385227334 1103 40-41 5.459670905766213 1103 42-43 5.304997161044842 1103 44-45 5.066199025893535 1103 46-47 5.015518566806978 1103 48-49 5.034349528302872 1103 50-51 4.883678671435202 1103 52-53 4.722506457074495 1103 54-55 4.983085940523569 1103 56-57 5.005978082184551 1103 58-59 5.0698148323749095 1103 60-61 4.765778467933082 1103 62-63 4.967517037310131 1103 64-65 4.953114110477003 1103 66-67 5.1238361865434925 1103 68-69 5.096261264800027 1103 70-71 4.891507792956354 1103 72-73 4.720194380780722 1103 74-75 4.860094716019248 1103 76-77 4.804394562201516 1103 78-79 4.8030167582852386 1103 80-81 4.656001006756707 1103 82-83 4.592756619446224 1103 84-85 4.668270330488088 1103 86-87 4.623541149640886 1103 88-89 4.381144944438823 1103 90-91 4.440672082645516 1103 92-93 4.427369629114917 1103 94-95 4.411863424328331 1103 96-97 4.33611344016499 1103 98-99 4.098548027988986 1103 100-101 4.1197541731278555 1104 1 4.391599060782646 1104 2 5.2642016417524395 1104 3 4.892256043207123 1104 4 6.376072016530994 1104 5 5.842009638198046 1104 6 5.569779064513039 1104 7 6.0337072693482625 1104 8 5.492464225847183 1104 9 6.237091009807081 1104 10-11 6.257703160519576 1104 12-13 6.121778196670011 1104 14-15 6.161060380167971 1104 16-17 6.180649756854496 1104 18-19 6.188896081350901 1104 20-21 5.566412814769585 1104 22-23 5.725631451413893 1104 24-25 5.48954747040775 1104 26-27 5.657186913791989 1104 28-29 5.603477198565411 1104 30-31 5.862643806688361 1104 32-33 5.725761593083529 1104 34-35 5.614513014407422 1104 36-37 5.721303015762217 1104 38-39 5.439956556610035 1104 40-41 5.766146066120296 1104 42-43 5.779810474564446 1104 44-45 5.6191163880247466 1104 46-47 5.615407513825819 1104 48-49 5.482510126344675 1104 50-51 5.23938782076841 1104 52-53 5.170322544230849 1104 54-55 5.496524948104547 1104 56-57 5.482820436754391 1104 58-59 5.279175314873548 1104 60-61 5.0825969391553585 1104 62-63 5.082652764954588 1104 64-65 5.306195554020302 1104 66-67 5.343605988765152 1104 68-69 5.243556111255526 1104 70-71 5.199497283398038 1104 72-73 5.144944049115871 1104 74-75 5.186383046807883 1104 76-77 5.063355363060467 1104 78-79 5.020882185281895 1104 80-81 4.843234925853409 1104 82-83 4.710197202385835 1104 84-85 4.672951978872277 1104 86-87 4.5949619242322655 1104 88-89 4.6926895057913995 1104 90-91 4.637426375869342 1104 92-93 4.548783628905266 1104 94-95 4.471282223871533 1104 96-97 4.434469558806381 1104 98-99 4.326094621939983 1104 100-101 4.331436111951007 1105 1 4.413486045785284 1105 2 5.053182348253145 1105 3 4.7085519173534784 1105 4 6.1572609781766126 1105 5 3.5609256973952945 1105 6 4.899023094663352 1105 7 5.321434043018819 1105 8 1.0490935487054962 1105 9 0.23550482630427183 1105 10-11 1.9616537015067905 1105 12-13 5.379416001813286 1105 14-15 3.5877421069838427 1105 16-17 5.645463381254945 1105 18-19 6.116355058606306 1105 20-21 3.166097797135386 1105 22-23 5.142984505448869 1105 24-25 5.40432919733265 1105 26-27 5.4881158310036895 1105 28-29 2.9646624121694707 1105 30-31 5.3409271959569224 1105 32-33 5.485792494544601 1105 34-35 5.575090274377402 1105 36-37 5.647381554164539 1105 38-39 5.474747476626611 1105 40-41 5.735042548149732 1105 42-43 2.6978814542880194 1105 44-45 4.849563928173655 1105 46-47 5.209780333520971 1105 48-49 5.157410362957439 1105 50-51 5.15545776060927 1105 52-53 5.1030613281170645 1105 54-55 5.385025553826285 1105 56-57 5.382163009484774 1105 58-59 5.34740227379767 1105 60-61 5.1081933565304425 1105 62-63 4.920351341731369 1105 64-65 5.0810312837849665 1105 66-67 5.323826090645955 1105 68-69 5.45027343035656 1105 70-71 5.171382802918991 1105 72-73 5.06552882697482 1105 74-75 5.135200152961922 1105 76-77 4.965951657873823 1105 78-79 4.879332688205064 1105 80-81 4.730762958401595 1105 82-83 4.70059201213072 1105 84-85 4.6299202737371985 1105 86-87 4.714009447077586 1105 88-89 4.69553090482167 1105 90-91 4.728999163522712 1105 92-93 4.720443291082557 1105 94-95 4.693194118856077 1105 96-97 4.558374496055865 1105 98-99 4.477009124199228 1105 100-101 4.617939833729341 1106 1 3.962035297637332 1106 2 4.250421730502026 1106 3 4.3526983790216285 1106 4 5.959697535206484 1106 5 5.839784236033278 1106 6 5.00256028421617 1106 7 5.58491375643105 1106 8 5.2002861519105785 1106 9 5.7921819568752255 1106 10-11 5.6752774462020525 1106 12-13 6.011640719435835 1106 14-15 5.6164033095134265 1106 16-17 5.650854985439164 1106 18-19 5.895571013022423 1106 20-21 5.2770864501597785 1106 22-23 5.256380205591899 1106 24-25 5.166493477279218 1106 26-27 5.29890489495957 1106 28-29 5.4381974064042105 1106 30-31 5.570662522701866 1106 32-33 5.313445720611259 1106 34-35 4.950253051624173 1106 36-37 5.112887645275933 1106 38-39 5.019988286429758 1106 40-41 5.207936591049052 1106 42-43 2.1859617136546916 1106 44-45 4.3998378719553735 1106 46-47 4.615284473238521 1106 48-49 4.493152243039642 1106 50-51 4.681616630920946 1106 52-53 4.361790599566 1106 54-55 4.723287164180309 1106 56-57 4.722892437129573 1106 58-59 4.759384816133462 1106 60-61 4.435487409439787 1106 62-63 4.504830949068186 1106 64-65 4.607299146753533 1106 66-67 4.7697087234445945 1106 68-69 4.710547719287554 1106 70-71 4.595520574698561 1106 72-73 4.3314210002335685 1106 74-75 4.350944035050496 1106 76-77 4.261665149955672 1106 78-79 4.429077063343943 1106 80-81 4.375706075200743 1106 82-83 4.36355385401594 1106 84-85 4.536003680780873 1106 86-87 4.587998997982147 1106 88-89 4.700499929607673 1106 90-91 4.590150813466277 1106 92-93 4.686207822376527 1106 94-95 4.578804093571609 1106 96-97 4.59886992514776 1106 98-99 4.45550776563968 1106 100-101 4.490497999289783 1107 1 2.4383030756903743 1107 2 -0.922460425432682 1107 3 0.08642405843501422 1107 4 1.8247063481660142 1107 5 1.6768059561326965 1107 6 3.2888101521607815 1107 7 4.343531953570743 1107 8 3.923486939732939 1107 9 4.201237712976493 1107 10-11 2.044830756112411 1107 12-13 4.326105711320995 1107 14-15 2.411763238441374 1107 16-17 0.911818177355336 1107 18-19 1.547708456887296 1107 20-21 1.413524470002372 1107 22-23 3.509048403998083 1107 24-25 3.518649393453238 1107 26-27 2.4238916276405433 1107 28-29 3.3455390063533095 1107 30-31 2.7554914437892712 1107 32-33 3.292096488324134 1107 34-35 3.889888201895488 1107 36-37 4.126360754474462 1107 38-39 4.085435677725776 1107 40-41 2.532015771150359 1107 42-43 3.243601619773351 1107 44-45 3.793586447147245 1107 46-47 3.8769352180926795 1107 48-49 1.3822377648885542 1107 50-51 3.2612487168894653 1107 52-53 3.4156030308740313 1107 54-55 3.982601934181531 1107 56-57 2.3198684150962485 1107 58-59 1.4652094626420364 1107 60-61 2.541605018122141 1107 62-63 3.5920834494713034 1107 64-65 3.7523684302688842 1107 66-67 3.907809621791909 1107 68-69 3.9590794178318234 1107 70-71 3.6966096995744593 1107 72-73 3.5798620053001713 1107 74-75 3.663194388588252 1107 76-77 3.5204565172710502 1107 78-79 3.4548129439201567 1107 80-81 3.4032696023368096 1107 82-83 3.3091632208976236 1107 84-85 3.3263265384409166 1107 86-87 3.2632727928184266 1107 88-89 3.340463689284789 1107 90-91 3.2452377723249235 1107 92-93 3.298727290892977 1107 94-95 3.3979259607699177 1107 96-97 3.4721366634383877 1107 98-99 3.4517300239690556 1107 100-101 3.403816225280881 1108 1 -1.655768485047851 1108 2 -1.036267739239996 1108 3 -1.7462943742834192 1108 4 -3.088653765194099 1108 5 -8.227837215177143 1108 6 -6.32998873330477 1108 7 -4.8994876894489 1108 8 -5.605235722323059 1108 9 -7.374697529625415 1108 10-11 -3.4482480369663797 1108 12-13 -1.7280870095383953 1108 14-15 -6.274037714026246 1108 16-17 -5.80624144070428 1108 18-19 -3.8828732070276963 1108 20-21 -4.351074327929759 1108 22-23 -3.2477070527573737 1108 24-25 -7.883538075400896 1108 26-27 -8.45974308932751 1108 28-29 -5.548536454388824 1108 30-31 -5.3613821397509795 1108 32-33 -2.1784112488502707 1108 34-35 -0.7698273578200769 1108 36-37 -4.024097062650018 1108 38-39 -5.068252209295444 1108 40-41 -2.8368305643626464 1108 42-43 -3.4570635475584837 1108 44-45 -1.4331645462704081 1108 46-47 -2.214544140053345 1108 48-49 -1.197081814431023 1108 50-51 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-9.169337385776583 2313 46-47 -9.998494757337294 2313 48-49 -7.4069759943252045 2313 50-51 -7.606957671316923 2313 52-53 -5.645772890501888 2313 54-55 -7.36680838522879 2313 56-57 -5.861384656156826 2313 58-59 -5.9564612990287245 2313 60-61 -4.908935522418396 2313 62-63 -6.95971016232231 2313 64-65 -8.676550488650033 2313 66-67 -10.496735832753544 2313 68-69 -9.940699451947044 2313 70-71 -10.914127401162643 2313 72-73 -9.245838240400076 2313 74-75 -10.144766299372435 2313 76-77 -8.43391694710241 2313 78-79 -8.861083861976649 2313 80-81 -10.401890102822893 2313 82-83 -9.686659874925475 2313 84-85 -9.863845451731072 2313 86-87 -9.47259944305381 2313 88-89 -11.185064566243469 2313 90-91 -9.924000556913409 2313 92-93 -9.60869285652717 2313 94-95 -10.290378707534753 2313 96-97 -10.211499700197978 2313 98-99 -9.725518133279103 2313 100-101 -8.786650605185951 2314 1 -10.555063017675717 2314 2 -11.524098427070683 2314 3 -10.142855220844266 2314 4 -25.077013553553886 2314 5 -16.828189948863212 2314 6 -15.061558398207769 2314 7 -14.24975223971345 2314 8 -8.69350732726133 2314 9 -11.36473280299402 2314 10-11 -9.594808883527225 2314 12-13 -10.845106409891127 2314 14-15 -12.305210553532419 2314 16-17 -14.134680594143434 2314 18-19 -12.290456981278137 2314 20-21 -13.159980853502951 2314 22-23 -13.249162079212397 2314 24-25 -14.724102449298602 2314 26-27 -12.987697233948317 2314 28-29 -14.14346590765161 2314 30-31 -15.347845984548158 2314 32-33 -13.001515305287661 2314 34-35 -13.541520479513196 2314 36-37 -14.404740801627092 2314 38-39 -10.905862438572342 2314 40-41 -15.202791763657178 2314 42-43 -15.793130566958837 2314 44-45 -11.214337385776584 2314 46-47 -10.94016142400396 2314 48-49 -10.418642660991871 2314 50-51 -9.901957671316925 2314 52-53 -9.022439557168553 2314 54-55 -9.420141718562125 2314 56-57 -7.776384656156825 2314 58-59 -6.446461299028723 2314 60-61 -7.357268855751734 2314 62-63 -11.836376828988975 2314 64-65 -8.874883821983367 2314 66-67 -8.338402499420212 2314 68-69 -12.194032785280378 2314 70-71 -12.779127401162643 2314 72-73 -10.465838240400073 2314 74-75 -8.324766299372435 2314 76-77 -9.710583613769078 2314 78-79 -10.084417195309983 2314 80-81 -10.126890102822893 2314 82-83 -10.411659874925473 2314 84-85 -10.80551211839774 2314 86-87 -11.39759944305381 2314 88-89 -10.331731232910135 2314 90-91 -9.792333890246741 2314 92-93 -11.082026189860503 2314 94-95 -10.745378707534751 2314 96-97 -10.99983303353131 2314 98-99 -11.238851466612438 2314 100-101 -10.676650605185952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79900.0 3 13673.0 4 9311.0 5 7525.0 6 7761.0 7 8875.0 8 10613.0 9 12201.0 10 13341.0 11 14636.0 12 15760.0 13 17018.0 14 18998.0 15 21117.0 16 22910.0 17 24260.0 18 22422.0 19 20452.0 20 20364.0 21 21876.0 22 25116.0 23 30284.0 24 36916.0 25 45177.0 26 52929.0 27 60856.0 28 67519.0 29 75041.0 30 84076.0 31 94192.0 32 108630.0 33 129384.0 34 156533.0 35 190871.0 36 235085.0 37 284708.0 38 256914.0 39 63153.0 40 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.85712911137321 20.39183126725612 14.523606138440549 23.227433482930124 2 14.605564024390244 20.64026919652493 41.35013928609972 23.404027492985108 3 17.65735095626516 26.19608235218502 30.890891210476635 25.255675481073187 4 11.354144653395643 17.671526353286268 40.329788853976986 30.644540139341103 5 12.23788456122226 38.011616877941954 35.90231617660989 13.848182384225902 6 29.270609507390198 38.61547841809811 18.648403170531715 13.465508903979984 7 25.79488939297979 32.90404301650463 23.278065569385813 18.023002021129763 8 24.605301726911396 35.92941292245994 21.163632876880552 18.301652473748113 9 25.16315914971784 16.440773407382082 21.932652142043395 36.46341530085668 10-11 23.40367764506214 26.98996518361411 29.69964952275999 19.90670764856376 12-13 24.317729071091506 24.695863746958636 29.36344524200872 21.62296193994114 14-15 21.793825706594884 26.26615074024226 27.04367849932705 24.8963450538358 16-17 21.002294190504028 29.219828030339674 27.894601818109177 21.883275961047126 18-19 20.78659083804317 28.28027026674515 29.98687710974969 20.946261785461992 20-21 21.75375898467094 27.529790848488535 29.958919108050296 20.75753105879023 22-23 21.523056490614824 27.382656106541464 29.682218610233868 21.412068792609848 24-25 21.283112551453048 27.64327484642403 29.748387289388887 21.325225312734034 26-27 21.13291992473484 27.75680533722899 29.759402254588785 21.350872483447386 28-29 21.135205766007036 27.68908460685054 29.834052345909956 21.341657281232468 30-31 21.34794079905047 27.536205673207025 29.81565000292312 21.300203524819388 32-33 21.258892880061026 27.774359910278523 29.594609142204504 21.37213806745595 34-35 21.51314726688969 27.83094075400324 29.597567932313368 21.058344046793703 36-37 21.49048386774485 27.465460178638473 29.51922419622678 21.524831757389897 38-39 21.29634946224686 27.854045794462305 29.55912339980713 21.290481343483705 40-41 21.370548555856477 27.633585378572512 29.63690346022507 21.358962605345937 42-43 21.351797585724285 27.67961979194703 29.447430794800024 21.521151827528666 44-45 21.43025629985687 27.88293189948484 29.227185881727678 21.4596259189306 46-47 21.50519435986266 27.75310113154693 29.11627238111777 21.62543212747264 48-49 21.44617247766604 27.909175583079804 29.18289982990658 21.461752109347568 50-51 21.25287952193734 28.05162054176006 28.880611730496508 21.814888205806092 52-53 21.33573036466987 28.24749776040437 28.726679770307157 21.690092104618603 54-55 21.390735104646986 28.131545374768628 28.73687237882135 21.740847141763034 56-57 21.39773135817996 28.21533172975404 28.564609676520657 21.822327235545345 58-59 21.30843520674179 28.189205697239018 28.788390372746864 21.713968723272327 60-61 21.464082237201985 28.322182668922046 28.553990254740086 21.659744839135886 62-63 21.367559785738802 28.22245625738565 28.660607050993107 21.749376905882436 64-65 21.275056617232224 28.082042160776556 28.689887118408674 21.953014103582543 66-67 21.340275131812813 28.174062229175128 28.515466975066573 21.970195663945482 68-69 21.384594315634086 28.026422635298488 28.558588910794096 22.030394138273333 70-71 21.40566168121358 27.89884219808029 28.611603896559444 22.083892224146688 72-73 21.479639010795818 27.975403063022718 28.559786158887846 21.985171767293618 74-75 21.358254857571584 28.181455761262846 28.490744852148264 21.969544529017305 76-77 21.379007157863253 28.17353712036208 28.413406826162518 22.03404889561215 78-79 21.427653238304043 28.134490029023812 28.290867433549582 22.146989299122563 80-81 21.387156846641762 28.197209025654296 28.255097021204733 22.16053710649921 82-83 21.528873213107218 28.041377734294098 28.446551694442125 21.983197358156556 84-85 21.524189242494828 28.046733844187195 28.183514187809998 22.24556272550798 86-87 21.293393416983868 28.145307270572605 28.357451231044095 22.20384808139943 88-89 21.545508660304588 27.903421146930068 28.291896646823155 22.25917354594219 90-91 21.466007186009083 27.926168860711314 28.40842879461482 22.199395158664778 92-93 21.63652051978211 27.88777290430123 28.31619868269103 22.159507893225634 94-95 21.290536825040885 27.83547206652163 28.40090923641197 22.47308187202552 96-97 21.38974038200196 27.725094194018883 28.405530193966793 22.479635230012363 98-99 21.298665509466872 27.770295560646076 28.205883823245852 22.7251551066412 100-101 21.37984733196413 27.656851052675137 28.17273895496625 22.790562660394485 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 443.0 1 306.0 2 191.5 3 235.0 4 224.5 5 227.5 6 354.5 7 660.5 8 1124.0 9 1469.5 10 1435.0 11 1348.5 12 1441.0 13 1607.0 14 1747.5 15 1888.0 16 2130.5 17 2375.5 18 2655.5 19 3000.0 20 3388.5 21 3916.5 22 4806.5 23 6224.5 24 7972.0 25 10423.0 26 14330.0 27 18992.0 28 23617.0 29 29181.0 30 35259.0 31 41607.5 32 49451.0 33 57544.0 34 65519.5 35 72323.5 36 79916.0 37 87084.5 38 88139.0 39 87780.0 40 88194.5 41 87617.5 42 86995.0 43 87727.0 44 88331.5 45 88824.5 46 87756.0 47 84981.0 48 83045.5 49 80762.5 50 77737.5 51 74181.0 52 70293.5 53 65960.5 54 61563.0 55 56567.5 56 51162.5 57 45182.5 58 39853.5 59 34635.5 60 28647.5 61 23070.5 62 17966.5 63 13764.0 64 10521.5 65 7828.0 66 5716.5 67 4393.5 68 3439.0 69 2601.0 70 2040.0 71 1577.0 72 1206.5 73 939.0 74 731.0 75 570.5 76 432.5 77 338.0 78 276.0 79 195.5 80 141.5 81 114.0 82 95.0 83 82.5 84 61.5 85 49.5 86 43.5 87 33.5 88 25.5 89 19.0 90 15.5 91 10.5 92 7.0 93 6.0 94 4.5 95 2.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02125640475219275 2 0.35131880028179435 3 0.0 4 0.4087447000767499 5 0.21516858723464677 6 0.09699809994627086 7 0.10842446771820057 8 0.11048289426534967 9 0.10779433714254268 10-11 0.12545899761348547 12-13 0.1704293163629367 14-15 0.1201028877203934 16-17 0.11401162548903385 18-19 0.15526417384210356 20-21 0.10878154171107338 22-23 0.09525473868695071 24-25 0.09746019570175332 26-27 0.1594230356414456 28-29 0.11178516412170932 30-31 0.120228913835525 32-33 0.10220717937170941 34-35 0.1422834839835511 36-37 0.1391118267527397 38-39 0.1347009127231345 40-41 0.10834045030811285 42-43 0.10934865922916547 44-45 0.09036072454934112 46-47 0.09470862552138054 48-49 0.09888849167324454 50-51 0.08510963641885871 52-53 0.09407849494572265 54-55 0.3092470821803694 56-57 0.3297683345942946 58-59 0.3424549635175401 60-61 0.16463211506688416 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2380459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.05220486104155 #Duplication Level Percentage of deduplicated Percentage of total 1 84.15252156780004 56.42612115737345 2 9.718924841467896 13.033506789983424 3 2.6806873021492876 5.392379824563204 4 1.1056970068707728 2.965576888757581 5 0.6021222190056342 2.0186811190075353 6 0.34785115382268983 1.3994512096361205 7 0.2390489779707081 1.122013272990017 8 0.1672662998184133 0.8972459361418115 9 0.12680871202629954 0.7652523363257033 >10 0.7740949538841367 9.607905111888874 >50 0.05935348091964458 2.7345459132930237 >100 0.024721451617037586 2.793319499811967 >500 7.087399372150192E-4 0.29325749295003317 >1k 1.288618067660216E-4 0.2979665481543628 >5k 6.44309033830108E-5 0.2527768991229776 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5851 0.2457929332116201 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4299 0.18059542298355066 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2598 0.10913861570394617 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.2008705043859187E-5 2 0.0 0.0 0.0 0.0 4.2008705043859187E-5 3 0.0 0.0 0.0 0.0 4.2008705043859187E-5 4 0.0 4.2008705043859187E-5 0.0 0.0 4.2008705043859187E-5 5 0.0 4.2008705043859187E-5 0.0 0.0 4.2008705043859187E-5 6 0.0 4.2008705043859187E-5 0.0 0.0 4.2008705043859187E-5 7 0.0 4.2008705043859187E-5 0.0 0.0 4.2008705043859187E-5 8 0.0 4.2008705043859187E-5 0.0 0.0 4.2008705043859187E-5 9 0.0 4.2008705043859187E-5 0.0 0.0 8.401741008771837E-5 10-11 0.0 4.2008705043859187E-5 0.0 0.0 8.401741008771837E-5 12-13 0.0 4.2008705043859187E-5 0.0 0.0 8.401741008771837E-5 14-15 0.0 4.2008705043859187E-5 0.0 0.0 8.401741008771837E-5 16-17 0.0 4.2008705043859187E-5 0.0 4.2008705043859187E-5 8.401741008771837E-5 18-19 0.0 4.2008705043859187E-5 0.0 4.2008705043859187E-5 1.2602611513157756E-4 20-21 2.1004352521929593E-5 4.2008705043859187E-5 0.0 4.2008705043859187E-5 1.6803482017543675E-4 22-23 4.2008705043859187E-5 4.2008705043859187E-5 0.0 2.1004352521929593E-4 1.6803482017543675E-4 24-25 4.2008705043859187E-5 4.2008705043859187E-5 0.0 5.67117518092099E-4 1.6803482017543675E-4 26-27 4.2008705043859187E-5 4.2008705043859187E-5 0.0 0.0010082089210526205 1.6803482017543675E-4 28-29 4.2008705043859187E-5 4.2008705043859187E-5 0.0 0.002688557122806988 1.6803482017543675E-4 30-31 4.2008705043859187E-5 4.2008705043859187E-5 0.0 0.007897636548245528 1.6803482017543675E-4 32-33 4.2008705043859187E-5 4.2008705043859187E-5 0.0 0.02014317406853048 1.6803482017543675E-4 34-35 6.301305756578878E-5 4.2008705043859187E-5 0.0 0.041609622345942524 1.6803482017543675E-4 36-37 8.401741008771837E-5 4.2008705043859187E-5 0.0 0.07626680400712635 1.6803482017543675E-4 38-39 8.401741008771837E-5 4.2008705043859187E-5 0.0 0.1239256798793846 1.6803482017543675E-4 40-41 8.401741008771837E-5 8.401741008771837E-5 0.0 0.17347494747861653 2.1004352521929593E-4 42-43 8.401741008771837E-5 8.401741008771837E-5 0.0 0.2268890159418835 2.1004352521929593E-4 44-45 8.401741008771837E-5 8.401741008771837E-5 0.0 0.29231757404769415 2.3104787774122553E-4 46-47 8.401741008771837E-5 8.401741008771837E-5 0.0 0.37047476978179417 2.520522302631551E-4 48-49 8.401741008771837E-5 8.401741008771837E-5 0.0 0.4511104791134819 2.520522302631551E-4 50-51 8.401741008771837E-5 8.401741008771837E-5 0.0 0.534623784740674 2.520522302631551E-4 52-53 8.401741008771837E-5 8.401741008771837E-5 0.0 0.6190822862313529 2.520522302631551E-4 54-55 8.401741008771837E-5 8.401741008771837E-5 0.0 0.7088128802050361 2.520522302631551E-4 56-57 8.401741008771837E-5 8.401741008771837E-5 0.0 0.8170273043980174 2.520522302631551E-4 58-59 8.401741008771837E-5 8.401741008771837E-5 0.0 0.9372982269385862 2.520522302631551E-4 60-61 8.401741008771837E-5 8.401741008771837E-5 0.0 1.0634713725378173 2.520522302631551E-4 62-63 8.401741008771837E-5 8.401741008771837E-5 0.0 1.1901486225975746 2.940609353070143E-4 64-65 8.401741008771837E-5 8.401741008771837E-5 0.0 1.323085169708867 2.940609353070143E-4 66-67 8.401741008771837E-5 8.401741008771837E-5 0.0 1.4787694306014092 3.150652878289439E-4 68-69 8.401741008771837E-5 8.401741008771837E-5 0.0 1.6495768253097407 3.360696403508735E-4 70-71 8.401741008771837E-5 8.401741008771837E-5 0.0 1.8335119403442781 3.360696403508735E-4 72-73 8.401741008771837E-5 8.401741008771837E-5 2.1004352521929593E-5 2.0202616386167542 3.780783453947327E-4 74-75 8.401741008771837E-5 1.2602611513157756E-4 4.2008705043859187E-5 2.21245146419241 3.780783453947327E-4 76-77 8.401741008771837E-5 1.2602611513157756E-4 4.2008705043859187E-5 2.4244904029012893 3.780783453947327E-4 78-79 8.401741008771837E-5 1.2602611513157756E-4 4.2008705043859187E-5 2.6630998475504093 3.780783453947327E-4 80-81 8.401741008771837E-5 1.2602611513157756E-4 4.2008705043859187E-5 2.9181346958716787 3.9908269791666227E-4 82-83 8.401741008771837E-5 1.2602611513157756E-4 4.2008705043859187E-5 3.1827055202379038 4.2008705043859187E-4 84-85 8.401741008771837E-5 1.2602611513157756E-4 4.2008705043859187E-5 3.447549401186914 4.6209575548245105E-4 86-87 8.401741008771837E-5 1.2602611513157756E-4 4.2008705043859187E-5 3.7224333626414063 4.6209575548245105E-4 88-89 8.401741008771837E-5 1.2602611513157756E-4 4.2008705043859187E-5 4.027059487266952 4.6209575548245105E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2935 0.0 56.192844 1 GTATCAA 5860 0.0 43.073124 1 ATCAACG 6880 0.0 35.116604 3 CAACGCA 6920 0.0 35.11422 5 TCAACGC 7065 0.0 34.542328 4 AACGCAG 7245 0.0 33.917137 6 TATCAAC 7420 0.0 33.2449 2 ACGCAGA 8250 0.0 28.810253 7 CGCAGAG 8275 0.0 28.321087 8 GTACATG 6640 0.0 23.542345 1 GCAGAGT 9420 0.0 23.364748 9 ACATGGG 6290 0.0 22.652365 3 TACATGG 6725 0.0 22.321152 2 GAGTACT 5645 0.0 21.601173 12-13 AGAGTAC 8440 0.0 20.500626 10-11 CAGAGTA 9255 0.0 20.390236 10-11 GTACTTT 6310 0.0 19.701775 14-15 GTACTAG 745 0.0 19.25019 1 GTATAGG 915 0.0 18.285929 1 AGTACTT 6460 0.0 17.956066 12-13 >>END_MODULE