FastQCFastQC Report
Sat 18 Jun 2016
SRR1931136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380459
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74780.31414109631797904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59270.2489855947949534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.16673255031907713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37150.054.6367571
GTATCAA67550.040.674141
ATCAACG83650.032.530053
TCAACGC85550.031.6945614
CAACGCA86100.031.6575585
TATCAAC88750.030.500192
AACGCAG89650.030.4569386
GTGGTAT18050.027.9154431
ACGCAGA103950.026.0843647
CGCAGAG104550.025.4804748
TGGTATC18800.025.0076772
GTACATG73800.023.1235221
GCAGAGT115200.022.6714279
TACATGG73300.022.4165172
GAGTACT72500.021.02498412-13
ACATGGG74850.020.6833953
CAGAGTA112800.020.2911510-11
AGAGTAC107350.019.684610-11
GTACTTT78350.019.30363514-15
AGTACTT81700.017.93088712-13
Produced by FastQC (version 0.11.5)