FastQCFastQC Report
Sat 18 Jun 2016
SRR1931135_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931135_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638576
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57530.21803427303212036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43690.16558173802839107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30900.055.2705271
GTATCAA58950.041.6207661
ATCAACG66850.035.019133
CAACGCA69350.034.509945
TCAACGC69600.034.3297734
AACGCAG72600.033.277576
TATCAAC74150.032.5050052
ACGCAGA80600.029.4491377
CGCAGAG82000.028.7717728
GCAGAGT88200.025.0239839
GTACATG61600.023.1171721
TACATGG63050.021.7462042
GAGTACT57600.021.68041412-13
CAGAGTA89950.020.54456310-11
ACATGGG61650.020.5363583
AGAGTAC82700.020.21747410-11
GTACTTT66750.018.85192914-15
CTACACG3305.529728E-1018.8252014
GTGGTAT15400.017.806471
CATGGGG41900.017.6777864
Produced by FastQC (version 0.11.5)