##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931135_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2638576 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.562424201539013 31.0 28.0 31.0 22.0 34.0 2 28.237008901771258 31.0 28.0 33.0 16.0 34.0 3 28.28330584375815 31.0 28.0 34.0 16.0 34.0 4 31.482550057303637 35.0 32.0 37.0 17.0 37.0 5 31.59087477487857 35.0 32.0 37.0 17.0 37.0 6 31.879683207912148 35.0 32.0 37.0 19.0 37.0 7 31.511754825330026 35.0 32.0 37.0 17.0 37.0 8 31.723542926184425 35.0 32.0 37.0 18.0 37.0 9 32.641958010684554 37.0 32.0 39.0 17.0 39.0 10-11 32.712291592131514 37.0 31.5 39.0 17.0 39.0 12-13 32.69847599614337 37.0 32.0 39.0 17.0 39.0 14-15 33.49299887515084 38.0 32.0 40.0 17.0 41.0 16-17 33.490576356337655 38.0 32.0 40.0 17.0 41.0 18-19 33.509120070826086 38.0 32.0 40.0 17.5 41.0 20-21 33.77282879098423 38.0 32.0 40.0 19.0 41.0 22-23 33.6541947247303 38.0 32.0 40.0 18.5 41.0 24-25 33.56021865582041 38.0 32.0 40.0 18.5 41.0 26-27 33.203415402853665 38.0 31.0 40.0 17.0 41.0 28-29 33.15111200132193 37.0 31.0 40.0 17.0 41.0 30-31 32.86525174942848 37.0 30.5 40.0 16.0 41.0 32-33 32.70660386511513 37.0 30.0 40.0 16.0 41.0 34-35 32.67910134102637 37.0 30.0 40.0 16.0 41.0 36-37 32.32141124606606 37.0 30.0 40.0 13.5 41.0 38-39 32.16842171686547 36.0 30.0 40.0 11.5 41.0 40-41 32.01600427654917 36.5 30.0 40.0 9.0 41.0 42-43 32.268051024491996 37.0 30.0 40.0 9.0 41.0 44-45 32.282415401337694 37.0 30.0 40.0 9.0 41.0 46-47 32.06201242639969 37.0 30.0 40.0 8.5 41.0 48-49 31.846651565086624 37.0 30.0 40.0 7.5 41.0 50-51 31.32813400106724 36.0 29.5 39.5 6.5 40.5 52-53 31.308021826924826 36.0 30.0 39.5 2.0 40.5 54-55 31.279801301914368 36.0 29.5 40.0 2.0 41.0 56-57 31.046329535325114 36.0 29.0 40.0 2.0 41.0 58-59 30.885870825778753 36.0 29.0 40.0 2.0 41.0 60-61 30.732113268672194 35.0 29.0 39.5 2.0 41.0 62-63 30.624945993596548 35.0 29.0 39.0 2.0 41.0 64-65 30.29490831418159 35.0 28.0 39.0 2.0 40.5 66-67 30.05887683356477 35.0 28.0 39.0 2.0 40.0 68-69 29.873935410615424 34.5 28.0 39.0 2.0 40.0 70-71 29.60296633487154 34.0 28.0 38.0 2.0 40.0 72-73 29.27397581119513 34.0 28.0 37.5 2.0 40.0 74-75 28.859939793282436 34.0 27.5 37.0 2.0 39.5 76-77 28.557303826003114 34.0 27.0 36.5 2.0 39.0 78-79 28.139369682737964 33.5 26.5 36.0 2.0 39.0 80-81 27.735326175937324 33.0 26.0 36.0 2.0 38.5 82-83 27.38799034024413 33.0 26.0 35.0 2.0 37.0 84-85 27.048695584284857 33.0 26.0 35.0 2.0 37.0 86-87 26.72620439964587 33.0 25.5 35.0 2.0 36.0 88-89 26.36688975417043 32.5 25.0 35.0 2.0 36.0 90-91 25.984159258630413 32.0 24.0 35.0 2.0 36.0 92-93 25.625301109386275 32.0 22.5 35.0 2.0 35.0 94-95 25.3297492662709 32.0 20.5 35.0 2.0 35.0 96-97 24.936224501397724 32.0 18.5 35.0 2.0 35.0 98-99 24.588331926008575 32.0 9.5 34.5 2.0 35.0 100-101 23.998358584327306 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.196098868307708 1101 2 5.861559019445092 1101 3 5.512601096065104 1101 4 6.773245583864952 1101 5 6.213487072141838 1101 6 5.689230040786715 1101 7 6.200209471774823 1101 8 5.610456643523683 1101 9 6.26908936061912 1101 10-11 6.278420094032615 1101 12-13 6.160271125575591 1101 14-15 6.509334785604874 1101 16-17 6.547512226726997 1101 18-19 6.416528510378093 1101 20-21 5.778917236362709 1101 22-23 5.8625544231551 1101 24-25 5.812884751444582 1101 26-27 6.025355970676387 1101 28-29 5.996645442906271 1101 30-31 6.027874778546234 1101 32-33 5.861501677367745 1101 34-35 5.682126444213722 1101 36-37 5.862665190497157 1101 38-39 5.744700878518977 1101 40-41 5.75552311768422 1101 42-43 5.759610776768643 1101 44-45 5.602740318783788 1101 46-47 5.569093958758824 1101 48-49 5.551697266405888 1101 50-51 5.496772599361403 1101 52-53 5.3174948576112655 1101 54-55 5.402737748366384 1101 56-57 5.399887113033493 1101 58-59 5.299741059373126 1101 60-61 5.254852106774482 1101 62-63 5.09734696401901 1101 64-65 5.148062625453992 1101 66-67 5.175178369704518 1101 68-69 5.204781946205774 1101 70-71 5.081921473561145 1101 72-73 4.826749493542071 1101 74-75 4.9646648773935524 1101 76-77 4.887299093689538 1101 78-79 4.9188958516847165 1101 80-81 4.792606090737365 1101 82-83 4.7168082557791955 1101 84-85 4.580369033062084 1101 86-87 4.337932292507979 1101 88-89 4.414569312450361 1101 90-91 4.316552520586487 1101 92-93 4.206213414345751 1101 94-95 4.172094254801799 1101 96-97 4.150249036233994 1101 98-99 3.97162862349634 1101 100-101 3.8870937350096746 1102 1 5.225513918029783 1102 2 5.7751251156404635 1102 3 5.528484384775883 1102 4 6.8955576849840945 1102 5 6.359533691262435 1102 6 5.811241249034662 1102 7 6.250851798242987 1102 8 5.9692503009356415 1102 9 6.713646962536515 1102 10-11 6.615021671666245 1102 12-13 6.679527464313345 1102 14-15 6.96873809953895 1102 16-17 6.860923961797379 1102 18-19 6.8327777328453365 1102 20-21 6.18862287840075 1102 22-23 6.27214085347082 1102 24-25 5.998606609588936 1102 26-27 6.2143160267682305 1102 28-29 6.0482487083184395 1102 30-31 6.308216542430749 1102 32-33 6.115135346346211 1102 34-35 6.214187842367814 1102 36-37 6.218614763961622 1102 38-39 6.023986872293932 1102 40-41 6.196801350713947 1102 42-43 6.130816460621634 1102 44-45 6.015627278470404 1102 46-47 6.066443079223337 1102 48-49 5.851301257162625 1102 50-51 5.80978987542392 1102 52-53 5.515688359558084 1102 54-55 5.776714604501869 1102 56-57 5.710570410797281 1102 58-59 5.686139257162367 1102 60-61 5.715325198137993 1102 62-63 5.555442376085843 1102 64-65 5.461167763522926 1102 66-67 5.541358093784698 1102 68-69 5.572573593095822 1102 70-71 5.446229322895192 1102 72-73 5.260346816170529 1102 74-75 5.341971726572762 1102 76-77 5.266528602774606 1102 78-79 5.128843680053322 1102 80-81 5.056159955253417 1102 82-83 4.9090986729231645 1102 84-85 4.749844608206317 1102 86-87 4.812839754494782 1102 88-89 4.766775389428332 1102 90-91 4.780458969354154 1102 92-93 4.9035298142630594 1102 94-95 4.805038095707346 1102 96-97 4.790892509968359 1102 98-99 4.394527458814142 1102 100-101 4.350178996024692 1103 1 4.938152662451955 1103 2 5.538811701272003 1103 3 5.1976075915439495 1103 4 6.463383162626261 1103 5 6.086184963077294 1103 6 5.484959808943199 1103 7 5.960425930768491 1103 8 5.461072556759127 1103 9 6.047786072685362 1103 10-11 6.068736353868768 1103 12-13 6.216001164253271 1103 14-15 6.399531010801521 1103 16-17 6.328064522243249 1103 18-19 6.063474479207926 1103 20-21 5.517450645021569 1103 22-23 5.797648643812561 1103 24-25 5.428535988030308 1103 26-27 5.514573462083121 1103 28-29 5.433758702132337 1103 30-31 5.746539525243577 1103 32-33 5.514239861337973 1103 34-35 5.475411537038362 1103 36-37 5.414916267246905 1103 38-39 5.259234446748785 1103 40-41 5.408971374086029 1103 42-43 5.338554435384058 1103 44-45 5.083160593561118 1103 46-47 4.950955343791673 1103 48-49 4.921002378347055 1103 50-51 4.916120384649648 1103 52-53 4.557428601853495 1103 54-55 4.9473175615541365 1103 56-57 5.050973792547175 1103 58-59 5.082523418265815 1103 60-61 4.955474209553071 1103 62-63 4.819220105756628 1103 64-65 4.795851436871157 1103 66-67 5.0567557988785765 1103 68-69 5.007504618618825 1103 70-71 4.857558851419341 1103 72-73 4.682779234970877 1103 74-75 4.680410759839393 1103 76-77 4.673019029406024 1103 78-79 4.620432288672951 1103 80-81 4.644673542089134 1103 82-83 4.573730671443872 1103 84-85 4.5743645821659555 1103 86-87 4.507021129153991 1103 88-89 4.384474903753251 1103 90-91 4.515469974289324 1103 92-93 4.61640878685229 1103 94-95 4.466165915408467 1103 96-97 4.417102038080159 1103 98-99 4.2249286278877385 1103 100-101 4.359796958685504 1104 1 4.340247698084479 1104 2 5.251038147135791 1104 3 4.851819896219315 1104 4 6.261464930174714 1104 5 5.8759389635355745 1104 6 5.490519378927129 1104 7 5.9686046785499975 1104 8 5.468973150194145 1104 9 6.043470960468184 1104 10-11 6.101176707594167 1104 12-13 6.006495121103228 1104 14-15 5.982636351355961 1104 16-17 6.038600962897444 1104 18-19 6.131123969922605 1104 20-21 5.475921939574178 1104 22-23 5.517241558230747 1104 24-25 5.365631217996633 1104 26-27 5.430783737390541 1104 28-29 5.459756056126551 1104 30-31 5.69470456775759 1104 32-33 5.562317931584364 1104 34-35 5.406320256331703 1104 36-37 5.480460212071609 1104 38-39 5.313413403563125 1104 40-41 5.595603020989721 1104 42-43 5.486537394789391 1104 44-45 5.401420792907018 1104 46-47 5.447063279581624 1104 48-49 5.244957437369969 1104 50-51 5.186961941098385 1104 52-53 4.940995735435308 1104 54-55 5.083148278144485 1104 56-57 5.113526800361022 1104 58-59 5.11568319032083 1104 60-61 4.905737470986498 1104 62-63 4.862673504916366 1104 64-65 5.000696422071574 1104 66-67 4.963277158868916 1104 68-69 4.946606266199748 1104 70-71 4.768464174605477 1104 72-73 4.788208862822998 1104 74-75 4.774108149372797 1104 76-77 4.77171866572677 1104 78-79 4.825959042846641 1104 80-81 4.700711678226863 1104 82-83 4.551314086861822 1104 84-85 4.494615918447568 1104 86-87 4.549162279161017 1104 88-89 4.559112502772187 1104 90-91 4.655046125500682 1104 92-93 4.605625402491675 1104 94-95 4.360252324243326 1104 96-97 4.489662380078798 1104 98-99 4.2666413192017 1104 100-101 4.2214486950152015 1105 1 4.44894124158381 1105 2 5.2578708792973785 1105 3 4.83271356054804 1105 4 6.372494193126183 1105 5 3.8357822251462643 1105 6 4.893525187469972 1105 7 5.705055301204194 1105 8 1.2429507836371734 1105 9 0.915589030011116 1105 10-11 2.192816380498808 1105 12-13 5.446870935352024 1105 14-15 3.524880827196636 1105 16-17 5.741139993178134 1105 18-19 6.12134572562227 1105 20-21 3.2706828520188935 1105 22-23 5.314785708382669 1105 24-25 5.384140611739792 1105 26-27 5.709655681679116 1105 28-29 3.066085275138107 1105 30-31 5.433296804264884 1105 32-33 5.641825520629364 1105 34-35 5.5591847065310915 1105 36-37 5.707688452941493 1105 38-39 5.727737396671245 1105 40-41 5.828937684206288 1105 42-43 3.077453069940283 1105 44-45 5.210300011047522 1105 46-47 5.383542356718653 1105 48-49 5.366420171998477 1105 50-51 5.401313775724287 1105 52-53 5.362979587139918 1105 54-55 5.5657716868280716 1105 56-57 5.5914637148584205 1105 58-59 5.575790403284891 1105 60-61 5.436627737123022 1105 62-63 5.204552180421487 1105 64-65 5.253641557828278 1105 66-67 5.3378747795751735 1105 68-69 5.443217913697094 1105 70-71 5.29968081774647 1105 72-73 5.036002833870828 1105 74-75 5.255896850211659 1105 76-77 5.137527227923687 1105 78-79 5.175147541643618 1105 80-81 5.058597774695514 1105 82-83 4.850144119396287 1105 84-85 4.817850885587262 1105 86-87 4.802576118105204 1105 88-89 4.7540105473276455 1105 90-91 4.712467454662697 1105 92-93 4.711402630345411 1105 94-95 4.73679078586451 1105 96-97 4.681816814579825 1105 98-99 4.658380184906651 1105 100-101 4.740620467393963 1106 1 4.063627983101753 1106 2 4.379091388681953 1106 3 4.529345491129025 1106 4 6.06808124447819 1106 5 6.0472754206380515 1106 6 5.060464756439433 1106 7 5.693763405623098 1106 8 5.27863245725284 1106 9 6.073525976666936 1106 10-11 5.910719279324628 1106 12-13 6.139251290432448 1106 14-15 5.799347810857608 1106 16-17 5.8367042844899935 1106 18-19 5.960509923394817 1106 20-21 5.351679944089057 1106 22-23 5.311755588370314 1106 24-25 5.385455160254974 1106 26-27 5.597311825967651 1106 28-29 5.530859337855851 1106 30-31 5.725172094715724 1106 32-33 5.55478705110372 1106 34-35 5.07274317484287 1106 36-37 5.337816260236256 1106 38-39 5.463319134372043 1106 40-41 5.455281939780413 1106 42-43 2.5638674383387574 1106 44-45 4.572366491785978 1106 46-47 4.856548443318669 1106 48-49 4.813840016492364 1106 50-51 4.921054882498925 1106 52-53 4.646786339225663 1106 54-55 4.956286849180046 1106 56-57 4.844830594209974 1106 58-59 4.771129444578449 1106 60-61 4.522293463834739 1106 62-63 4.5975884411819266 1106 64-65 4.695677418131023 1106 66-67 4.9629530116347595 1106 68-69 4.92841927608632 1106 70-71 4.77135792626337 1106 72-73 4.5734862101160125 1106 74-75 4.626174320390053 1106 76-77 4.639922536201738 1106 78-79 4.703475877685342 1106 80-81 4.6595922248159205 1106 82-83 4.628418337120319 1106 84-85 4.647273369650776 1106 86-87 4.722579277734308 1106 88-89 4.888652101984174 1106 90-91 4.948845496251987 1106 92-93 4.8774619956705365 1106 94-95 4.849109858421137 1106 96-97 4.852602235301397 1106 98-99 4.770291808117012 1106 100-101 4.824805869193465 1107 1 2.8046140732083664 1107 2 -0.18876726529168764 1107 3 0.459904628380734 1107 4 2.371805110935181 1107 5 2.248310224160079 1107 6 4.029083005851913 1107 7 4.798891097419624 1107 8 4.323595330573134 1107 9 4.781169524622079 1107 10-11 2.6069836333277934 1107 12-13 4.7981152885139124 1107 14-15 2.8130764683651073 1107 16-17 1.4878523670397392 1107 18-19 1.9739643764570438 1107 20-21 1.8867167327012524 1107 22-23 4.220432407979079 1107 24-25 4.15581340088826 1107 26-27 3.0188851815993587 1107 28-29 3.9141244150065546 1107 30-31 3.1427910641063512 1107 32-33 3.744824782607335 1107 34-35 4.407141491266664 1107 36-37 4.603128367860048 1107 38-39 4.432012234044386 1107 40-41 2.8513613074673927 1107 42-43 3.676121329152455 1107 44-45 4.031960345929917 1107 46-47 4.259287159919321 1107 48-49 1.878275155863335 1107 50-51 3.6971555167452657 1107 52-53 3.8768431821082565 1107 54-55 4.464637275171526 1107 56-57 2.9519532540828948 1107 58-59 1.900587274089947 1107 60-61 3.1119479641207732 1107 62-63 3.986593059501068 1107 64-65 4.117150304324291 1107 66-67 4.349230323131749 1107 68-69 4.232131204534337 1107 70-71 3.892550744652972 1107 72-73 3.957174716824273 1107 74-75 4.191108003693692 1107 76-77 3.9307540924796776 1107 78-79 3.8026016034080037 1107 80-81 3.8958407741629486 1107 82-83 3.76876864901401 1107 84-85 3.803647077929149 1107 86-87 3.909314776354833 1107 88-89 3.8877863322176545 1107 90-91 3.88812777892608 1107 92-93 4.069699226094709 1107 94-95 4.085787542214973 1107 96-97 4.206061484696441 1107 98-99 4.197246036417173 1107 100-101 4.233280587818292 1108 1 -1.6848016319091066 1108 2 -0.8407827704088717 1108 3 -1.508850639094181 1108 4 -3.162678490933235 1108 5 -8.241977825749746 1108 6 -6.493964625949591 1108 7 -5.226751589105394 1108 8 -5.2812230170518255 1108 9 -7.604811677679528 1108 10-11 -3.1203657514602448 1108 12-13 -1.522462907378653 1108 14-15 -6.158750055399846 1108 16-17 -5.572583892692808 1108 18-19 -3.749256254140672 1108 20-21 -4.3166679156024585 1108 22-23 -3.2030297804666112 1108 24-25 -7.791229753468588 1108 26-27 -8.234667298098572 1108 28-29 -5.6536437331275735 1108 30-31 -5.417223797632893 1108 32-33 -2.196001987155068 1108 34-35 -0.8587930297442838 1108 36-37 -4.166831486521108 1108 38-39 -5.246067719492661 1108 40-41 -2.8618418412999276 1108 42-43 -3.32408987930636 1108 44-45 -1.232741058293378 1108 46-47 -1.9504108891316996 1108 48-49 -1.0033552395229748 1108 50-51 -0.23805545153852847 1108 52-53 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-4.685759146254295 2313 52-53 -2.56473586416044 2313 54-55 -3.7764006742122156 2313 56-57 -2.8455582127295465 2313 58-59 -3.104729739499401 2313 60-61 -1.3433314673644894 2313 62-63 -4.201108196937003 2313 64-65 -4.342865326414454 2313 66-67 -6.4125076783544515 2313 68-69 -5.537659189821518 2313 70-71 -5.538539478020994 2313 72-73 -4.385593290555484 2313 74-75 -4.8117982033324544 2313 76-77 -3.9668837868646847 2313 78-79 -4.642929969830121 2313 80-81 -5.30486474442738 2313 82-83 -5.2717324741784175 2313 84-85 -4.661241414259752 2313 86-87 -4.368765034368025 2313 88-89 -6.5917314210558295 2313 90-91 -6.2764412653781 2313 92-93 -5.14887273936392 2313 94-95 -5.594672753146199 2313 96-97 -6.512854271600695 2313 98-99 -5.922318438244801 2313 100-101 -5.27939483092322 2314 1 -12.26936060852203 2314 2 -14.026930957968148 2314 3 -12.360700092007734 2314 4 -25.113482349454134 2314 5 -18.37110641998047 2314 6 -16.873034232001082 2314 7 -16.994037438882167 2314 8 -12.37859746476054 2314 9 -14.303099863850848 2314 10-11 -12.699894540004973 2314 12-13 -14.144640149507781 2314 14-15 -15.737919131714357 2314 16-17 -16.40352724090813 2314 18-19 -14.784964921933124 2314 20-21 -15.644768264905009 2314 22-23 -15.438014397274774 2314 24-25 -16.322783802833598 2314 26-27 -15.121258475317571 2314 28-29 -16.162135717674985 2314 30-31 -16.93654184801574 2314 32-33 -13.656669594548253 2314 34-35 -15.799955212538244 2314 36-37 -15.411010162872634 2314 38-39 -14.549602383722693 2314 40-41 -17.933943902416303 2314 42-43 -17.646897485311587 2314 44-45 -13.888849226421254 2314 46-47 -12.627783717557062 2314 48-49 -12.246364561968555 2314 50-51 -12.263131210866758 2314 52-53 -12.560731520465584 2314 54-55 -13.075572657380398 2314 56-57 -11.5008453038114 2314 58-59 -10.677452693211904 2314 60-61 -11.513244593467379 2314 62-63 -14.024577723202782 2314 64-65 -11.673698772864842 2314 66-67 -12.737164933682266 2314 68-69 -16.347079578039246 2314 70-71 -16.310834849271163 2314 72-73 -14.644484290962705 2314 74-75 -13.438376186229155 2314 76-77 -13.858698636871473 2314 78-79 -13.361472117244265 2314 80-81 -13.416409470910347 2314 82-83 -13.68187228685658 2314 84-85 -13.793690172234504 2314 86-87 -14.551132348064236 2314 88-89 -13.605339402595128 2314 90-91 -12.997596416728715 2314 92-93 -13.010485336079 2314 94-95 -12.953332994764226 2314 96-97 -12.885495781034656 2314 98-99 -12.800287760899884 2314 100-101 -12.144502744592284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94361.0 3 14838.0 4 9909.0 5 8187.0 6 8398.0 7 9840.0 8 11300.0 9 12873.0 10 14313.0 11 15725.0 12 17376.0 13 18787.0 14 20612.0 15 23119.0 16 24829.0 17 26037.0 18 24811.0 19 22524.0 20 22399.0 21 24211.0 22 28035.0 23 34296.0 24 41833.0 25 50434.0 26 59445.0 27 68057.0 28 75019.0 29 81964.0 30 92881.0 31 103104.0 32 117587.0 33 140231.0 34 169707.0 35 207101.0 36 253945.0 37 313159.0 38 297077.0 39 80146.0 40 106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.503901918451135 20.487867753681567 14.589668303760133 23.418562024107164 2 14.356797641108315 20.761881996611496 41.48080632123098 23.400514041049206 3 17.57432797084488 26.07451898296657 30.902691451752762 25.448461594435784 4 11.247482921630414 17.91995776610463 40.53309612368977 30.29946318857518 5 12.290639729513732 37.758364435175224 35.98190171121251 13.969094124098536 6 28.993429637947255 38.56813905496039 19.071500106218327 13.366931200874024 7 25.557869896120256 32.67154575185716 23.531114601591963 18.23946975043062 8 24.15118922099755 36.1371118371206 21.63153327917982 18.080165662702026 9 24.773365870611205 16.56916106483705 22.15733230743782 36.500140757113925 10-11 23.102497196416348 27.08545535531449 29.952312982608763 19.859734465660395 12-13 24.157686432993827 24.71556948210873 29.52803014274042 21.598713942157026 14-15 21.504449729047113 26.38526347184735 27.31503353091777 24.795253268187768 16-17 20.740841359506803 29.33202784700236 28.111824352012338 21.815306441478498 18-19 20.549707500178492 28.412301702438587 30.092523671142395 20.945467126240523 20-21 21.539930944902775 27.671559112916356 30.087425012911307 20.701084929269562 22-23 21.20297834034306 27.579351154869375 29.788126318472862 21.429544186314704 24-25 21.046430021130423 27.731306026911884 29.91748893213607 21.304775019821626 26-27 20.946320087867615 27.893144533415974 29.847530546301403 21.313004832415007 28-29 20.852715090072998 27.90096523045636 29.97133523546463 21.27498444400601 30-31 21.13643771412483 27.69715231288018 29.926099542504716 21.240310430490275 32-33 21.050605867062643 27.90272058297692 29.683227481983522 21.36344606797692 34-35 21.32688291287568 28.00716316990573 29.689240481587873 20.976713435630717 36-37 21.2080369063459 27.66767210855129 29.68354007637209 21.44075090873072 38-39 21.078925362893717 27.958617185245288 29.819758502844383 21.142698949016612 40-41 21.267538035436505 27.68110938270668 29.75932389877452 21.292028683082293 42-43 21.169979051419915 27.83590715243293 29.569955604489746 21.42415819165741 44-45 21.197711941933992 27.973400955108897 29.426094385335244 21.402792717621868 46-47 21.29143405128773 27.957564581944784 29.232645220467603 21.518356146299883 48-49 21.239437431869312 28.09853193367194 29.301254903939405 21.36077573051934 50-51 21.148337754520107 28.185165257304725 29.055779022466027 21.61071796570914 52-53 21.196290660988705 28.368795940454227 28.92375150645428 21.511161892102784 54-55 21.1558755550149 28.165960860045008 28.97398272611155 21.70418085882854 56-57 21.171756922462713 28.298742407391565 28.844003383998253 21.68549728614747 58-59 21.163266656475383 28.30484717256506 28.973992450844165 21.55789372011539 60-61 21.31436293788056 28.393944455410967 28.75637745418103 21.535315152527446 62-63 21.202970844879964 28.334563795016702 28.84025322749847 21.622212132604858 64-65 21.078661368859567 28.30090927833801 28.87784926414854 21.742580088653877 66-67 21.222242603586178 28.242184420687522 28.721344391823468 21.814228583902835 68-69 21.201208530661994 28.23739016803003 28.724869020259412 21.836532281048566 70-71 21.24979534415533 28.09911482557258 28.729644323301663 21.92144550697043 72-73 21.319605726725325 28.168830460066342 28.69367795356283 21.817885859645507 74-75 21.19298439764479 28.385197166956722 28.647516690821114 21.77430174457738 76-77 21.248961561084464 28.349003401834928 28.55420878534482 21.847826251735786 78-79 21.28697448926997 28.337235690766533 28.519057249061614 21.85673257090188 80-81 21.281232755850123 28.45515914644869 28.477045952058987 21.786562145642193 82-83 21.506107839986417 28.30346747639636 28.590383600851364 21.600041082765856 84-85 21.538208488214856 28.357189635621637 28.34428494763842 21.760316928525082 86-87 21.352729654177104 28.31633426514908 28.483848863932664 21.84708721674115 88-89 21.51738286105839 28.220562909690685 28.423664885908156 21.838389343342772 90-91 21.536370375535896 28.22361379774545 28.498686412671077 21.741329414047577 92-93 21.594375147807 28.2670463158916 28.396908029179375 21.741670507122024 94-95 21.40080482805877 28.217891013940854 28.415687097889165 21.96561706011121 96-97 21.463300659143417 28.124090418468146 28.453245235308742 21.959363687079698 98-99 21.401657560744887 28.22840805040294 28.245273207972787 22.124661180879386 100-101 21.493582144308142 28.102810000545748 28.276445325054123 22.12716253009199 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 301.0 1 202.5 2 143.0 3 183.0 4 168.5 5 169.5 6 276.5 7 501.5 8 868.5 9 1168.5 10 1194.5 11 1203.0 12 1351.0 13 1504.0 14 1708.0 15 1966.5 16 2243.0 17 2568.5 18 2879.0 19 3209.5 20 3733.5 21 4429.5 22 5533.0 23 7214.5 24 9381.0 25 12416.5 26 16729.5 27 21913.0 28 27773.0 29 34841.0 30 42599.5 31 50658.5 32 59180.5 33 67591.5 34 75399.0 35 82349.0 36 90259.5 37 97086.5 38 99597.5 39 100336.5 40 100852.0 41 100721.0 42 99924.5 43 99629.5 44 99315.5 45 98999.0 46 97050.5 47 93904.0 48 91262.0 49 87728.0 50 83014.0 51 78425.0 52 74025.5 53 68963.0 54 64390.5 55 58763.0 56 52684.0 57 46974.5 58 41048.0 59 35607.0 60 29915.0 61 23978.0 62 18791.0 63 14450.0 64 10840.5 65 8095.0 66 6005.5 67 4549.5 68 3534.5 69 2726.0 70 2164.5 71 1668.5 72 1269.0 73 1016.0 74 792.0 75 603.0 76 473.5 77 394.5 78 300.5 79 208.5 80 168.0 81 132.0 82 106.5 83 82.5 84 61.5 85 60.5 86 55.0 87 36.5 88 31.5 89 30.5 90 25.0 91 16.0 92 11.0 93 8.0 94 5.0 95 4.5 96 2.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02922030671089254 2 0.7473349261116602 3 0.0 4 0.6437184299409985 5 0.27162378495067035 6 0.09459647931308403 7 0.10748221768105219 8 0.10835389998241475 9 0.10725482229808805 10-11 0.15165377082183723 12-13 0.22872185603143516 14-15 0.12305880141409609 16-17 0.11491046685788092 18-19 0.20511063543365815 20-21 0.12465056909484509 22-23 0.09387639393369757 24-25 0.09724942544766571 26-27 0.20935534924898885 28-29 0.1112721407304546 30-31 0.1189656845207415 32-33 0.10329435271146255 34-35 0.1405682459023352 36-37 0.13575504362959415 38-39 0.1316050778904985 40-41 0.10899818690081316 42-43 0.10750116729629923 44-45 0.09632089430056212 46-47 0.09205723086998442 48-49 0.09692728198846651 50-51 0.08404154362049833 52-53 0.09167823856504417 54-55 0.3038002316400968 56-57 0.32407631995439967 58-59 0.33646936832594554 60-61 0.16281509420232732 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2638576.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.07910481472743 #Duplication Level Percentage of deduplicated Percentage of total 1 85.80338229223352 62.7043436799226 2 9.322297255896391 13.625302765553965 3 2.3031564610509774 5.0493783726558314 4 0.876104852987274 2.5609983352059342 5 0.46250346093881356 1.6899669449560875 6 0.2752965743395313 1.2071056326776406 7 0.17387867142495073 0.8894828357876668 8 0.12112163241616038 0.7081168376537176 9 0.09060508757524278 0.5959204822492853 >10 0.5225175533396622 7.002957296871404 >50 0.035193767474722054 1.7272861564693462 >100 0.013571385918820749 1.6439400948771907 >500 2.1200251654438917E-4 0.10500349659542019 >1k 1.0600125825680874E-4 0.2673696558170996 >5k 5.300062912840437E-5 0.22282741270690626 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5753 0.21803427303212036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4369 0.16558173802839107 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.789923049402405E-5 3.789923049402405E-5 2 0.0 0.0 0.0 3.789923049402405E-5 3.789923049402405E-5 3 0.0 0.0 0.0 3.789923049402405E-5 3.789923049402405E-5 4 0.0 0.0 0.0 7.57984609880481E-5 3.789923049402405E-5 5 0.0 0.0 0.0 7.57984609880481E-5 3.789923049402405E-5 6 0.0 0.0 0.0 7.57984609880481E-5 7.57984609880481E-5 7 0.0 0.0 0.0 7.57984609880481E-5 7.57984609880481E-5 8 0.0 0.0 0.0 7.57984609880481E-5 7.57984609880481E-5 9 0.0 0.0 0.0 1.8949615247012026E-4 7.57984609880481E-5 10-11 0.0 0.0 0.0 2.6529461345816834E-4 7.57984609880481E-5 12-13 0.0 0.0 0.0 2.6529461345816834E-4 7.57984609880481E-5 14-15 1.8949615247012026E-5 0.0 0.0 2.6529461345816834E-4 9.474807623506012E-5 16-17 7.57984609880481E-5 0.0 0.0 2.6529461345816834E-4 1.1369769148207214E-4 18-19 1.1369769148207214E-4 0.0 0.0 3.031938439521924E-4 1.1369769148207214E-4 20-21 1.1369769148207214E-4 0.0 0.0 3.221434591992044E-4 1.1369769148207214E-4 22-23 1.1369769148207214E-4 0.0 0.0 4.1689153543426454E-4 1.1369769148207214E-4 24-25 1.515969219760962E-4 0.0 0.0 6.442869183984089E-4 1.1369769148207214E-4 26-27 1.7054653722310822E-4 0.0 0.0 0.0011369769148207214 1.1369769148207214E-4 28-29 1.8949615247012026E-4 0.0 0.0 0.0027287445955697315 1.7054653722310822E-4 30-31 1.8949615247012026E-4 0.0 0.0 0.007485098022569749 1.8949615247012026E-4 32-33 1.8949615247012026E-4 0.0 0.0 0.01965075101115147 1.8949615247012026E-4 34-35 2.0844576771713227E-4 0.0 0.0 0.04077957201156988 1.8949615247012026E-4 36-37 2.2739538296414428E-4 0.0 0.0 0.0737329529261238 1.8949615247012026E-4 38-39 2.6529461345816834E-4 0.0 0.0 0.11995106451358611 1.8949615247012026E-4 40-41 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.16690821109568194 1.8949615247012026E-4 42-43 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.21756053265094505 2.0844576771713227E-4 44-45 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.2828040579464075 2.2739538296414428E-4 46-47 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.36083857353360294 2.2739538296414428E-4 48-49 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.4372244725943084 2.463449982111563E-4 50-51 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.5177034885483686 2.6529461345816834E-4 52-53 2.8424422870518035E-4 3.789923049402405E-5 0.0 0.5965717872064326 2.8424422870518035E-4 54-55 3.031938439521924E-4 3.789923049402405E-5 0.0 0.6859571223265883 3.031938439521924E-4 56-57 3.031938439521924E-4 3.789923049402405E-5 0.0 0.7936856850058516 3.221434591992044E-4 58-59 3.031938439521924E-4 3.789923049402405E-5 0.0 0.9105858614646689 3.4109307444621644E-4 60-61 3.031938439521924E-4 3.789923049402405E-5 0.0 1.0325834844249324 3.4109307444621644E-4 62-63 3.4109307444621644E-4 3.789923049402405E-5 0.0 1.1554527896865583 3.4109307444621644E-4 64-65 3.4109307444621644E-4 3.789923049402405E-5 0.0 1.2881190460308893 3.4109307444621644E-4 66-67 3.4109307444621644E-4 3.789923049402405E-5 0.0 1.4412508868419938 3.4109307444621644E-4 68-69 3.789923049402405E-4 3.789923049402405E-5 0.0 1.6112668348381853 3.7899230494024046E-4 70-71 3.789923049402405E-4 3.789923049402405E-5 0.0 1.79020805161572 4.1689153543426454E-4 72-73 3.789923049402405E-4 3.789923049402405E-5 0.0 1.9748720521978522 4.5479076592828857E-4 74-75 3.789923049402405E-4 3.789923049402405E-5 0.0 2.168309724639351 4.737403811753006E-4 76-77 3.789923049402405E-4 9.474807623506012E-5 0.0 2.3792757911843356 4.926899964223126E-4 78-79 3.789923049402405E-4 1.1369769148207214E-4 0.0 2.6113517291144923 4.926899964223126E-4 80-81 3.789923049402405E-4 1.1369769148207214E-4 0.0 2.8642722438163615 5.116396116693247E-4 82-83 3.789923049402405E-4 1.1369769148207214E-4 0.0 3.127065508061924 5.305892269163367E-4 84-85 3.789923049402405E-4 1.1369769148207214E-4 0.0 3.388077508474268 5.495388421633486E-4 86-87 3.789923049402405E-4 1.1369769148207214E-4 0.0 3.661634154180134 5.684884574103607E-4 88-89 3.789923049402405E-4 1.515969219760962E-4 0.0 3.95681231088284 5.684884574103607E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3090 0.0 55.270527 1 GTATCAA 5895 0.0 41.620766 1 ATCAACG 6685 0.0 35.01913 3 CAACGCA 6935 0.0 34.50994 5 TCAACGC 6960 0.0 34.329773 4 AACGCAG 7260 0.0 33.27757 6 TATCAAC 7415 0.0 32.505005 2 ACGCAGA 8060 0.0 29.449137 7 CGCAGAG 8200 0.0 28.771772 8 GCAGAGT 8820 0.0 25.023983 9 GTACATG 6160 0.0 23.117172 1 TACATGG 6305 0.0 21.746204 2 GAGTACT 5760 0.0 21.680414 12-13 CAGAGTA 8995 0.0 20.544563 10-11 ACATGGG 6165 0.0 20.536358 3 AGAGTAC 8270 0.0 20.217474 10-11 GTACTTT 6675 0.0 18.851929 14-15 CTACACG 330 5.529728E-10 18.825201 4 GTGGTAT 1540 0.0 17.80647 1 CATGGGG 4190 0.0 17.677786 4 >>END_MODULE