FastQCFastQC Report
Sat 18 Jun 2016
SRR1931135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638576
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75350.28557070177247124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58930.22334016530128373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.14413077356877346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35600.054.5290261
GTATCAA64500.041.087821
ATCAACG78500.032.7293853
TCAACGC80200.032.1528324
CAACGCA80100.032.015115
TATCAAC84250.031.0593192
AACGCAG84600.030.4238746
ACGCAGA97150.026.0537347
CGCAGAG96600.025.7596368
GTGGTAT17050.023.4408041
GCAGAGT108800.022.4346759
GAGTACT72200.021.11316712-13
GTACATG68150.021.0842741
TGGTATC18750.020.0094932
ACATGGG67900.019.9335823
AGAGTAC103350.019.8958710-11
TACATGG71350.019.7018952
CAGAGTA110200.019.65028410-11
GTACTTT79800.019.34042514-15
AGTACTT81450.017.63680312-13
Produced by FastQC (version 0.11.5)