##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931135_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2638576 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.268908684078077 31.0 31.0 34.0 30.0 34.0 2 31.287750286518182 31.0 31.0 34.0 28.0 34.0 3 31.44990555511761 31.0 31.0 34.0 28.0 34.0 4 35.05549963313545 35.0 35.0 37.0 32.0 37.0 5 34.92891734026232 35.0 35.0 37.0 32.0 37.0 6 35.050585618909594 36.0 35.0 37.0 32.0 37.0 7 34.891927691300154 36.0 35.0 37.0 32.0 37.0 8 34.960521887563594 36.0 35.0 37.0 32.0 37.0 9 36.34754579743013 38.0 35.0 39.0 32.0 39.0 10-11 36.227523671859366 38.0 35.0 39.0 31.5 39.0 12-13 36.18089245865952 38.0 35.0 39.0 32.0 39.0 14-15 37.092254875357014 39.0 36.0 40.0 31.0 41.0 16-17 37.18477523482363 39.0 36.0 40.0 31.5 41.0 18-19 37.26024283552947 39.0 36.0 40.5 31.5 41.0 20-21 37.24004387214922 39.0 36.0 40.0 31.0 41.0 22-23 37.13496294971227 39.0 36.0 40.0 31.0 41.0 24-25 37.00690561878831 39.0 36.0 40.0 31.0 41.0 26-27 36.76139535112879 39.0 36.0 40.0 30.5 41.0 28-29 36.494890236248644 38.5 35.0 40.0 30.0 41.0 30-31 36.2919898839374 38.0 35.0 40.0 30.0 41.0 32-33 36.128489382151585 38.0 35.0 40.0 30.0 41.0 34-35 35.910010551145774 38.0 34.5 40.0 29.0 41.0 36-37 36.134372669197326 38.0 35.0 40.0 30.0 41.0 38-39 36.11569384395219 38.0 35.0 40.0 30.0 41.0 40-41 35.97086136613082 38.0 35.0 40.0 29.0 41.0 42-43 35.83667459265907 38.0 35.0 40.0 29.0 41.0 44-45 35.620536607624715 38.0 34.5 40.0 27.5 41.0 46-47 35.420493288804266 38.0 34.0 40.0 27.0 41.0 48-49 35.18844937572388 38.0 34.0 40.0 27.0 41.0 50-51 34.873566461606565 38.0 33.5 40.0 26.0 41.0 52-53 34.74162142761853 38.0 33.0 40.0 26.0 41.0 54-55 34.50683323125807 38.0 33.0 40.0 24.5 41.0 56-57 34.31184358532785 38.0 33.0 40.0 24.0 41.0 58-59 34.09431848845741 37.0 33.0 40.0 24.0 41.0 60-61 33.82367079818811 37.0 32.5 40.0 23.0 41.0 62-63 33.58309804227735 37.0 32.0 40.0 22.5 41.0 64-65 33.35712179599905 36.0 32.0 39.5 22.5 41.0 66-67 33.13213869905586 36.0 32.0 39.0 22.0 41.0 68-69 32.82737450048814 36.0 31.0 39.0 22.0 40.0 70-71 32.01707076089527 35.0 31.0 38.5 19.0 40.0 72-73 31.993211110841607 35.0 31.0 38.0 20.0 40.0 74-75 31.692637240693465 35.0 31.0 37.5 19.5 39.5 76-77 30.964569525380355 34.0 30.0 36.0 18.5 39.0 78-79 30.991767908144393 34.0 30.0 36.0 18.0 39.0 80-81 30.719449619794922 34.0 30.0 36.0 17.5 38.5 82-83 30.361193310331025 34.0 30.0 36.0 14.5 37.0 84-85 29.9970732319251 34.0 29.5 35.0 11.5 37.0 86-87 29.66389029537144 34.0 29.0 35.0 7.5 36.5 88-89 29.348276873586357 34.0 29.0 35.0 7.0 36.0 90-91 29.04066530583163 34.0 29.0 35.0 2.0 36.0 92-93 28.711404560641803 34.0 29.0 35.0 2.0 35.5 94-95 28.335981036741032 34.0 29.0 35.0 2.0 35.0 96-97 27.987135485201108 33.5 29.0 35.0 2.0 35.0 98-99 27.522718883215795 33.0 27.0 35.0 2.0 35.0 100-101 26.866107703549186 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8343173962909347 1101 2 0.7693548332543756 1101 3 0.5598885010125194 1101 4 0.8331386366634845 1101 5 0.7936562082483434 1101 6 0.9326662932589471 1101 7 0.8265569537800559 1101 8 0.8455964949872552 1101 9 1.2213943919814412 1101 10-11 1.0224158838355848 1101 12-13 1.1236142273038823 1101 14-15 1.4487341416118227 1101 16-17 1.3270321648387622 1101 18-19 1.0382391360805698 1101 20-21 0.9686907514842531 1101 22-23 0.8907946138985139 1101 24-25 0.8384098161164175 1101 26-27 1.0928281312681918 1101 28-29 1.0007280724742742 1101 30-31 1.1185820500838517 1101 32-33 1.2602176694804115 1101 34-35 1.0374766005045117 1101 36-37 1.3505288778242743 1101 38-39 1.1825277137867474 1101 40-41 1.2197553223380666 1101 42-43 1.1833900435202906 1101 44-45 1.157400719258007 1101 46-47 1.2853634615584255 1101 48-49 1.2386282057387987 1101 50-51 1.1350455318567398 1101 52-53 0.9642976319954641 1101 54-55 1.0381227251263851 1101 56-57 0.9069972533965895 1101 58-59 1.1152830080978617 1101 60-61 1.161125070819864 1101 62-63 1.006714351728867 1101 64-65 0.9894750707456978 1101 66-67 0.7669737375712415 1101 68-69 0.7217447878672445 1101 70-71 1.1023827150574732 1101 72-73 0.6623486709570656 1101 74-75 0.35368984537029746 1101 76-77 0.44626948528826205 1101 78-79 0.5774761045262942 1101 80-81 0.33699948212165154 1101 82-83 0.3392388651593983 1101 84-85 0.3320138892784499 1101 86-87 0.4173323062422263 1101 88-89 0.3479372131837728 1101 90-91 0.4071374075890972 1101 92-93 0.24457250733273028 1101 94-95 0.2120257256985525 1101 96-97 0.3012942650633619 1101 98-99 0.24475365761902879 1101 100-101 0.018506120206566834 1102 1 0.8885886536293661 1102 2 0.8122701332681679 1102 3 0.6456879583289243 1102 4 0.8611604065686222 1102 5 0.9361910611742417 1102 6 0.8758301553489005 1102 7 0.9497642353415401 1102 8 0.955187879616112 1102 9 1.3004728658174756 1102 10-11 1.0929916155029957 1102 12-13 1.2053155430351197 1102 14-15 1.4917142341052099 1102 16-17 1.3669128675544897 1102 18-19 1.2037602309202882 1102 20-21 1.269835639428912 1102 22-23 0.9129628651327053 1102 24-25 0.7320817920096587 1102 26-27 0.9672035635055636 1102 28-29 1.1927702679498466 1102 30-31 1.40917744812036 1102 32-33 1.4963557819303475 1102 34-35 1.5408674759385477 1102 36-37 1.6407516443513686 1102 38-39 1.4378784179754334 1102 40-41 1.4973452113807753 1102 42-43 1.5798204724055651 1102 44-45 1.529053372317989 1102 46-47 1.5350638797171783 1102 48-49 1.4207712281568519 1102 50-51 1.3437987819459636 1102 52-53 1.240384643718457 1102 54-55 1.0310823103514792 1102 56-57 1.2071570748462719 1102 58-59 1.1294247430385198 1102 60-61 1.363804319203176 1102 62-63 1.3034752015596354 1102 64-65 1.2860590925384159 1102 66-67 0.9260379639619174 1102 68-69 0.9514633617205988 1102 70-71 1.2153824095184902 1102 72-73 0.6996346154188942 1102 74-75 0.6716667016897873 1102 76-77 0.6703952054232083 1102 78-79 0.7174278834651204 1102 80-81 0.5472181416749606 1102 82-83 0.6787105403345706 1102 84-85 0.6395917481264384 1102 86-87 0.6523300066497129 1102 88-89 0.4299927830047636 1102 90-91 0.6021368495963557 1102 92-93 0.5337416197985831 1102 94-95 0.5467442043297304 1102 96-97 0.6205877404491673 1102 98-99 0.4879799109337526 1102 100-101 0.45060737367079184 1103 1 0.6449399043943451 1103 2 0.47976728987788064 1103 3 0.3096455231737316 1103 4 0.5436587077136821 1103 5 0.6165771032366294 1103 6 0.6136511165174454 1103 7 0.6551886333743937 1103 8 0.5551443348092775 1103 9 0.8627456672672338 1103 10-11 0.7050004835119736 1103 12-13 0.8698784644526256 1103 14-15 1.1502578522230564 1103 16-17 0.9116148194129465 1103 18-19 0.8764481797776469 1103 20-21 0.9352241956033325 1103 22-23 0.6704674642135231 1103 24-25 0.4307767179765847 1103 26-27 0.820306225334896 1103 28-29 0.5918506910938177 1103 30-31 0.971972998947443 1103 32-33 1.1065397442426956 1103 34-35 1.0388858696846626 1103 36-37 1.2364292539172652 1103 38-39 0.9468626522886439 1103 40-41 1.000059099357685 1103 42-43 1.0583917057176393 1103 44-45 0.9919190679290182 1103 46-47 1.1043015032034305 1103 48-49 0.9279944335350976 1103 50-51 0.763253429951412 1103 52-53 0.46782165032455225 1103 54-55 0.5478031111930264 1103 56-57 0.695142237949689 1103 58-59 0.85359236854692 1103 60-61 0.8769218816217688 1103 62-63 0.660957241282695 1103 64-65 0.5980745921333934 1103 66-67 0.5192270260145051 1103 68-69 0.36589666577554425 1103 70-71 0.7982509105527171 1103 72-73 0.34011712519761517 1103 74-75 0.15938075672191587 1103 76-77 0.3198550924276091 1103 78-79 0.1678372653967024 1103 80-81 0.09201501127184031 1103 82-83 0.18432131768807025 1103 84-85 0.2685700439257275 1103 86-87 0.215004983186045 1103 88-89 -0.04180070250436785 1103 90-91 -0.0028707249542243574 1103 92-93 -0.21189428118728415 1103 94-95 -0.15405195875703015 1103 96-97 -0.08566788385827806 1103 98-99 -0.23361204983524075 1103 100-101 -0.5297987058138105 1104 1 0.45866022261121486 1104 2 0.3905335809798167 1104 3 0.19256919092984148 1104 4 0.6119820091843877 1104 5 0.40157853751929906 1104 6 0.6099270315613765 1104 7 0.573483037273256 1104 8 0.5264294187114373 1104 9 0.7993367565708809 1104 10-11 0.5940908227592772 1104 12-13 0.6840840986540044 1104 14-15 0.935431436496792 1104 16-17 0.8031642026984542 1104 18-19 0.6685498768663436 1104 20-21 0.6886880265655648 1104 22-23 0.45586367566925645 1104 24-25 0.41580332096669537 1104 26-27 0.14258056749243053 1104 28-29 0.5163469359425079 1104 30-31 0.7466402034362858 1104 32-33 0.913776753118114 1104 34-35 0.7481216265273574 1104 36-37 1.0739122902896128 1104 38-39 0.5850420574684918 1104 40-41 0.8901698939737273 1104 42-43 1.0871105445627975 1104 44-45 1.091535477349133 1104 46-47 1.043227979826959 1104 48-49 0.8452869587536256 1104 50-51 0.8403823748036245 1104 52-53 0.6042327306157915 1104 54-55 0.8334795961805881 1104 56-57 0.7130170927516772 1104 58-59 0.6852333069510479 1104 60-61 1.1060063340427675 1104 62-63 0.2642721701677999 1104 64-65 -0.7072201608448978 1104 66-67 -0.053532767394685266 1104 68-69 0.4073737442612355 1104 70-71 0.9815202444891611 1104 72-73 0.5050149709961289 1104 74-75 0.3026425319301005 1104 76-77 0.19380504737012316 1104 78-79 0.03361207968167079 1104 80-81 0.045605483847463546 1104 82-83 -0.1438789880697975 1104 84-85 -0.1604162586522726 1104 86-87 0.14655500403071642 1104 88-89 0.18214921185800037 1104 90-91 0.3047867393079713 1104 92-93 0.27914607378257017 1104 94-95 0.2838472991403407 1104 96-97 0.41841616952462957 1104 98-99 0.424681332345763 1104 100-101 0.10977196707803216 1105 1 -0.40636680914747103 1105 2 -1.8157435682638372 1105 3 -0.4070302095258178 1105 4 0.3117842042875054 1105 5 0.4783039104655131 1105 6 -0.08756880188935412 1105 7 -0.2646788117390386 1105 8 -0.32519368047857 1105 9 0.33296186843699616 1105 10-11 0.6231638088303484 1105 12-13 0.8610342577130581 1105 14-15 1.0363782846842753 1105 16-17 0.6504587470299157 1105 18-19 0.5018927452115634 1105 20-21 0.8073606948411367 1105 22-23 0.10824108749853423 1105 24-25 -0.30488442151744266 1105 26-27 0.7527197406692494 1105 28-29 0.21084139140479152 1105 30-31 0.6057400244719986 1105 32-33 0.7367597593611066 1105 34-35 0.34656660947294426 1105 36-37 0.5090623624151718 1105 38-39 0.6028415946691226 1105 40-41 0.8438551688971572 1105 42-43 1.1398867277950444 1105 44-45 1.1881291445383653 1105 46-47 1.3360554714599928 1105 48-49 1.3297228620737656 1105 50-51 0.7571328492063145 1105 52-53 1.0519121309682191 1105 54-55 0.12389533918778284 1105 56-57 0.34346438947903124 1105 58-59 -0.37589888035989816 1105 60-61 0.4355048361497609 1105 62-63 0.9629552739044982 1105 64-65 1.3684795172516644 1105 66-67 0.9287525426720791 1105 68-69 0.9583763615144676 1105 70-71 0.39785589210609373 1105 72-73 -0.08194546651896673 1105 74-75 -0.03421058293245238 1105 76-77 0.23881571618812103 1105 78-79 -0.2629501972447521 1105 80-81 -0.13512242182732592 1105 82-83 -0.10242007533214448 1105 84-85 3.927025096182035E-4 1105 86-87 -0.2776781067927203 1105 88-89 -0.7440825951780212 1105 90-91 -0.8315524419987028 1105 92-93 -0.4573835891017026 1105 94-95 -0.7039629230089481 1105 96-97 -0.7322640818813717 1105 98-99 -1.3458920867797346 1105 100-101 -0.7718056049394448 1106 1 0.3130687775587617 1106 2 0.33131860145525494 1106 3 -0.23080513920770684 1106 4 0.3886035942946293 1106 5 0.1252319595819813 1106 6 0.49703166568815504 1106 7 0.437651935379364 1106 8 0.538334798133242 1106 9 0.9917988631120451 1106 10-11 0.21886629672587077 1106 12-13 0.28166181598619744 1106 14-15 0.032444695208752705 1106 16-17 -0.21396296646490498 1106 18-19 0.45278484953049514 1106 20-21 0.5920169765824639 1106 22-23 0.5614817601960667 1106 24-25 0.36524762241746345 1106 26-27 0.7561336276854291 1106 28-29 0.6400643606588048 1106 30-31 0.3679321465824472 1106 32-33 0.3363958962355298 1106 34-35 -1.0025567868367702 1106 36-37 -0.866590463965295 1106 38-39 -0.7380048527117467 1106 40-41 0.4528266709467488 1106 42-43 0.7836015947722785 1106 44-45 0.5365924104285895 1106 46-47 0.20651270132806587 1106 48-49 0.0986449941748262 1106 50-51 0.6869201782010563 1106 52-53 -0.013667071888896487 1106 54-55 0.5856624287052483 1106 56-57 0.32540011750577236 1106 58-59 0.6936627344902462 1106 60-61 0.8585560560581555 1106 62-63 0.7500321826090683 1106 64-65 0.8341034071418605 1106 66-67 0.42796793119637755 1106 68-69 0.4670357088695738 1106 70-71 0.7611653090503907 1106 72-73 0.2539106114828158 1106 74-75 0.2774073018773464 1106 76-77 0.4032583451185374 1106 78-79 0.4453809890085374 1106 80-81 0.4152732458510968 1106 82-83 0.4159656338617843 1106 84-85 0.41913352783329927 1106 86-87 0.578563737526224 1106 88-89 0.4527368305311228 1106 90-91 0.7263245550088726 1106 92-93 0.23235582777714114 1106 94-95 0.46555430232496775 1106 96-97 0.7682888532117538 1106 98-99 0.7090865604348835 1106 100-101 0.5483616054888785 1107 1 0.38054462289921176 1107 2 0.3953569340851608 1107 3 0.16730675374321535 1107 4 -1.4056889809504796 1107 5 0.07987935780913347 1107 6 0.32063944821354795 1107 7 0.5428545302588006 1107 8 0.49961634867987925 1107 9 0.9565208844599482 1107 10-11 0.7240878022567117 1107 12-13 0.8957413770055354 1107 14-15 1.0476916984251474 1107 16-17 0.9754921796357223 1107 18-19 0.8361414690244189 1107 20-21 0.22830773154532835 1107 22-23 0.5824887471007045 1107 24-25 0.47783942227304976 1107 26-27 0.8556537302889211 1107 28-29 0.8259439145998542 1107 30-31 1.1809146785285094 1107 32-33 1.1494002969237016 1107 34-35 1.0320771732068081 1107 36-37 1.2220693254925976 1107 38-39 1.0431961638150042 1107 40-41 0.5492007384218525 1107 42-43 0.38109308101741846 1107 44-45 0.19079192202667627 1107 46-47 0.5691695527679954 1107 48-49 0.9053649839482816 1107 50-51 -0.5489747736586565 1107 52-53 0.8273871071273575 1107 54-55 1.0686023799918374 1107 56-57 1.10043525803885 1107 58-59 1.162139206840287 1107 60-61 1.4483047398398412 1107 62-63 1.4558239657725665 1107 64-65 1.3865563246380717 1107 66-67 1.0720702114089136 1107 68-69 1.0804778013384322 1107 70-71 1.593466761737055 1107 72-73 1.1596233185425646 1107 74-75 0.8291287786196193 1107 76-77 0.9032443470106628 1107 78-79 1.194112364261894 1107 80-81 1.2455204630008403 1107 82-83 1.1810354421616545 1107 84-85 1.0415304476156884 1107 86-87 1.1546165669135888 1107 88-89 1.153629328760232 1107 90-91 1.307352734520606 1107 92-93 1.2859781947462565 1107 94-95 1.2389687789040238 1107 96-97 1.3055343847780172 1107 98-99 1.3485241428718524 1107 100-101 1.2922661690305155 1108 1 0.5504953797416547 1108 2 0.5122687490950781 1108 3 0.2836388008058748 1108 4 0.6166791738794402 1108 5 0.4818892588424646 1108 6 0.5319722158012752 1108 7 0.5536893349673946 1108 8 0.5502881837189406 1108 9 0.8420159142925954 1108 10-11 0.6968825952622311 1108 12-13 0.8001297385606492 1108 14-15 0.92814137884681 1108 16-17 0.6859606810454721 1108 18-19 0.7151685855122167 1108 20-21 0.6326432545955711 1108 22-23 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1.7575231370131021 2311 74-75 1.797918756591983 2311 76-77 1.8730318974866336 2311 78-79 1.6733935631441703 2311 80-81 1.7324712976424266 2311 82-83 1.7112845398420724 2311 84-85 1.9650610825684076 2311 86-87 1.9071381860969439 2311 88-89 1.785003121227696 2311 90-91 2.1162908808277976 2311 92-93 2.082314380070329 2311 94-95 2.343535332924695 2311 96-97 2.308213897780522 2311 98-99 2.5384812241674766 2311 100-101 2.590254750304503 2312 1 0.003226866512672899 2312 2 -0.07712929552926795 2312 3 -0.3293951326330067 2312 4 0.2933738055734949 2312 5 -0.8911206812557921 2312 6 0.25295659564687156 2312 7 0.4939630549145093 2312 8 -1.984057385674042 2312 9 -4.556989259781535 2312 10-11 -0.37870091625293867 2312 12-13 -5.758959167375664 2312 14-15 -2.0600214983954217 2312 16-17 -2.6002162167951255 2312 18-19 -1.960690767810128 2312 20-21 -2.855428219444285 2312 22-23 -1.704115086317266 2312 24-25 -3.17047343261455 2312 26-27 -3.5023851990564197 2312 28-29 -6.330632320598561 2312 30-31 -6.211913288739687 2312 32-33 -1.4794363956100653 2312 34-35 0.6835249882137191 2312 36-37 1.2004181660686442 2312 38-39 1.2776598139035897 2312 40-41 -2.5599673395609273 2312 42-43 -4.685661979613641 2312 44-45 -3.4862195768484803 2312 46-47 0.43542820162718243 2312 48-49 -0.927417323318025 2312 50-51 -6.338388121913262 2312 52-53 -1.0171014640845542 2312 54-55 0.9690678270669082 2312 56-57 1.5786818404325231 2312 58-59 1.6321430903532814 2312 60-61 1.969521589230773 2312 62-63 1.8249455564962531 2312 64-65 1.7160425289752013 2312 66-67 1.3739277485263344 2312 68-69 1.3548118018393822 2312 70-71 1.883769985972016 2312 72-73 1.4114783279296361 2312 74-75 1.352092634373541 2312 76-77 1.4629007939869183 2312 78-79 1.2456932872511395 2312 80-81 1.4819155288711983 2312 82-83 0.52445662178447 2312 84-85 0.9245071603253479 2312 86-87 1.3091427553489936 2312 88-89 1.2367281768045721 2312 90-91 1.4979573905999146 2312 92-93 1.6325127913319832 2312 94-95 1.9274573225406897 2312 96-97 2.178015558931744 2312 98-99 2.069838712020214 2312 100-101 2.400661565803752 2313 1 -0.32528572806639033 2313 2 -0.6339485657141175 2313 3 -0.8506814078939975 2313 4 -1.8312550613932714 2313 5 -2.238932193563045 2313 6 -3.059776859003069 2313 7 -3.7116446454973264 2313 8 -3.9791967401420045 2313 9 -3.3144119204462257 2313 10-11 -2.102613855626565 2313 12-13 -4.133228873342524 2313 14-15 -2.3380480284212837 2313 16-17 -6.040260273877781 2313 18-19 -5.113609549536015 2313 20-21 -5.165994736061464 2313 22-23 -3.685486602455569 2313 24-25 -1.8974392404873512 2313 26-27 -4.284506164481186 2313 28-29 -6.613100474989906 2313 30-31 -3.4229443202500747 2313 32-33 -5.863954162107532 2313 34-35 -3.0080993772685147 2313 36-37 -5.384104389242147 2313 38-39 -3.695858048370141 2313 40-41 -2.5536461059626134 2313 42-43 -3.536298891786803 2313 44-45 -4.9345123648956 2313 46-47 -4.022945038483915 2313 48-49 -3.849012477038933 2313 50-51 -2.438558124786553 2313 52-53 -3.092656966431072 2313 54-55 -7.618328016243119 2313 56-57 -7.646464218374106 2313 58-59 -3.1900243265608097 2313 60-61 -7.739150951713583 2313 62-63 -4.180848907635532 2313 64-65 -4.602581166073172 2313 66-67 -4.429228079459488 2313 68-69 -5.754780191264729 2313 70-71 -5.99671320529319 2313 72-73 -2.1765400611135632 2313 74-75 -0.5516761617187882 2313 76-77 -3.0280124327154994 2313 78-79 -0.5009665154497505 2313 80-81 0.13884494176268092 2313 82-83 0.37335758912476535 2313 84-85 0.07752171831787891 2313 86-87 -0.19493970801658733 2313 88-89 -3.0801356293442623 2313 90-91 -0.7935616209890171 2313 92-93 0.16385557459028632 2313 94-95 0.15733233451272355 2313 96-97 0.7500153673792092 2313 98-99 1.0822058224502484 2313 100-101 1.469907806585283 2314 1 -28.80641780353809 2314 2 -14.846721743398387 2314 3 -5.380001348168193 2314 4 -4.10653672285655 2314 5 -1.7831157146706857 2314 6 -1.251781745659784 2314 7 -0.12646750419150266 2314 8 -4.496775130260101 2314 9 -3.2224477559287834 2314 10-11 -0.8293683011267632 2314 12-13 0.20232155423993703 2314 14-15 -1.3072688781565915 2314 16-17 -0.8022393698462906 2314 18-19 -3.9715232186007654 2314 20-21 -2.90466040729806 2314 22-23 -5.24826656716305 2314 24-25 -0.985331191077826 2314 26-27 -3.5499466422876225 2314 28-29 -2.409659454221618 2314 30-31 -4.496583522096149 2314 32-33 -3.5330189103089715 2314 34-35 -2.898387147052688 2314 36-37 -6.030738025729185 2314 38-39 -8.243502951315708 2314 40-41 -6.984656015016498 2314 42-43 -4.6213063575123385 2314 44-45 -4.437498655108712 2314 46-47 -6.813836853333921 2314 48-49 -5.405446575043548 2314 50-51 -2.421929917918085 2314 52-53 -3.578777503963309 2314 54-55 -4.246942106103308 2314 56-57 -1.7545814981352024 2314 58-59 -1.7754729487950982 2314 60-61 -0.677558716068134 2314 62-63 0.15324230995643262 2314 64-65 -2.18813159365563 2314 66-67 -0.9672693445606164 2314 68-69 -0.10044328343249731 2314 70-71 0.6221615062583226 2314 72-73 -0.03299452018781324 2314 74-75 0.42534976470988184 2314 76-77 -1.5613706518006119 2314 78-79 -3.1223863607056224 2314 80-81 -2.6589017665310593 2314 82-83 -2.463414502635789 2314 84-85 -0.6570717389917498 2314 86-87 0.38171971915865655 2314 88-89 0.15293074774274373 2314 90-91 0.029500683884066348 2314 92-93 0.29467544699425474 2314 94-95 0.36202895457516604 2314 96-97 0.4532867125672091 2314 98-99 0.8364137768414537 2314 100-101 0.756320186115623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 17.0 4 45.0 5 100.0 6 240.0 7 638.0 8 1550.0 9 2802.0 10 4301.0 11 6396.0 12 8379.0 13 10672.0 14 12855.0 15 15876.0 16 18121.0 17 20118.0 18 18547.0 19 15489.0 20 15135.0 21 15944.0 22 17557.0 23 20182.0 24 23749.0 25 29368.0 26 37845.0 27 47600.0 28 60887.0 29 76054.0 30 94033.0 31 115442.0 32 139105.0 33 168951.0 34 206690.0 35 254588.0 36 320296.0 37 399637.0 38 371853.0 39 87430.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.64857269679599 21.753354624823864 15.408093019335109 23.189979659045044 2 14.572708915718174 22.03779614458708 40.64097452565323 22.74852041404151 3 17.823424313931195 26.760227073745906 31.30185359671139 24.11449501561151 4 12.33230007045491 18.36394527787242 39.61612496480097 29.6876296868717 5 11.887597993606374 38.74985077115781 36.45943912694969 12.903112108286132 6 29.16008483363754 39.67746238880366 18.30244798709607 12.860004790462733 7 25.537714282249212 33.46358035546446 23.41937469301623 17.579330669270092 8 24.27646579063859 36.92268102188453 20.96562691391114 17.83522627356574 9 24.843135084985235 16.542900412950015 21.69298894555245 36.9209755565123 10-11 23.144036783477148 27.586395085834177 29.729937663345684 19.53963046734299 12-13 24.110201866461303 25.063064319542054 29.578568136752555 21.24816567724409 14-15 21.315550509062465 26.912508868419938 27.311625664752505 24.460314957765096 16-17 20.667151524155454 29.890573551794603 27.9879942817641 21.454280642285838 18-19 20.550232398081388 28.81419750653383 30.086152530759016 20.54941756462577 20-21 21.338384795435115 27.934575316382777 30.191493441917157 20.535546446264956 22-23 21.126111205438082 27.902493617769586 29.873765243070505 21.097629933721826 24-25 21.00950314828865 28.011931673768263 30.05520616689673 20.92335901104635 26-27 21.035801833916558 28.128076623865173 29.877508321986245 20.95861322023202 28-29 20.89947430858503 28.078876453042152 30.050048703206073 20.971600535166747 30-31 21.248483293440533 27.894761078092927 29.931236319321048 20.92551930914549 32-33 21.107177981887816 28.061817926857692 29.778913079318038 21.052091011936458 34-35 21.290294583538547 28.04195455674703 29.831593185805005 20.836157673909423 36-37 21.27147931075192 27.85852794889933 29.782079709113678 21.087913031235068 38-39 21.120302983101027 28.027054877977143 29.894491812399398 20.95815032652243 40-41 21.337705206320102 27.874954022755443 29.833750452666223 20.95359031825823 42-43 21.255999740766857 27.923426925720417 29.61988803326371 21.20068530024902 44-45 21.31236511368208 28.09533365585719 29.445935469835966 21.146365760624764 46-47 21.361072325318922 28.08905613853245 29.201708150298728 21.348163385849908 48-49 21.347635494281036 28.140530178902733 29.23343401780707 21.27840030900916 50-51 21.361657948934745 28.229633764222385 29.026453238230115 21.38225504861276 52-53 21.39856821628128 28.32195766804736 28.900386465630255 21.379087650041104 54-55 21.421948826827293 28.19682572781256 28.95252809121886 21.428697354141292 56-57 21.48703418614893 28.295251869016134 28.819933190679443 21.397780754155495 58-59 21.423235679469343 28.312413280541453 28.927053135885533 21.337297904103675 60-61 21.475059039428217 28.37836096465745 28.795436482443943 21.35114351347039 62-63 21.407536717887766 28.297276175518178 28.834059251937465 21.461127854656592 64-65 21.274578828024246 28.249181061071948 28.78617000006633 21.69007011083747 66-67 21.38812275885646 28.272946031387473 28.675115342734593 21.663815867021473 68-69 21.3336238997167 28.260429596081142 28.768554400659458 21.637392103542698 70-71 21.396900850447814 27.98846360918325 28.74800520839354 21.866630331975394 72-73 21.352142815316455 28.04357396076077 28.73577790651466 21.86850531740811 74-75 21.264318699104415 28.264049422571436 28.57836960923446 21.89326226908969 76-77 21.18618148577111 28.24699762296026 28.581136188610827 21.9856847026578 78-79 21.190047207281502 28.267235812044074 28.46404651599954 22.078670464674886 80-81 21.16685439504215 28.339846977371025 28.418033445465035 22.07526518212179 82-83 21.29530073399857 28.17058237425627 28.549614733182217 21.984502158562943 84-85 21.286270538556053 28.191420820488645 28.377013492894264 22.145295148061038 86-87 21.08528815234617 28.201809066251894 28.522099408253077 22.19080337314886 88-89 21.209075962502737 28.07680237833364 28.43428011367125 22.279841545492378 90-91 21.197754369262466 28.009479463310477 28.547768354237522 22.24499781318954 92-93 21.223492474500624 28.057101469928327 28.433243787369772 22.286162268201277 94-95 20.953326995279053 27.992414042586493 28.466043687853166 22.58821527428129 96-97 20.92563087250357 27.958479751137737 28.465970935749997 22.649918440608694 98-99 20.80019541180214 28.009833503914393 28.415987611285903 22.773983472997564 100-101 20.82384086382578 27.910134375846834 28.435035882197994 22.83098887812939 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 349.0 1 243.5 2 143.5 3 178.0 4 173.0 5 162.5 6 273.0 7 608.0 8 1053.5 9 1382.5 10 1415.5 11 1389.5 12 1506.0 13 1686.0 14 1895.0 15 2064.5 16 2322.5 17 2626.5 18 2916.0 19 3269.0 20 3815.5 21 4638.0 22 5750.5 23 7348.0 24 9483.0 25 12580.5 26 17102.5 27 22611.5 28 28620.5 29 35371.5 30 42968.0 31 50786.5 32 59370.0 33 67852.0 34 75821.5 35 83107.0 36 90684.0 37 97380.5 38 99922.0 39 100082.5 40 99991.5 41 99622.0 42 98967.0 43 98730.5 44 98459.5 45 98879.0 46 97096.5 47 93493.0 48 90895.5 49 87449.5 50 82843.5 51 78065.0 52 73634.5 53 69352.5 54 64910.0 55 58929.0 56 52624.5 57 46873.5 58 41330.0 59 35974.0 60 29872.0 61 23872.0 62 18666.0 63 14177.0 64 10555.0 65 7834.5 66 5788.5 67 4285.0 68 3303.0 69 2646.0 70 2073.0 71 1548.0 72 1185.5 73 906.5 74 725.0 75 581.5 76 449.0 77 326.5 78 228.5 79 158.5 80 107.0 81 84.0 82 78.5 83 65.5 84 44.5 85 30.0 86 21.5 87 17.5 88 13.5 89 11.5 90 7.5 91 3.0 92 5.5 93 3.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1884728732467816 2 0.0 3 0.005230093808175319 4 3.4109307444621644E-4 5 4.1689153543426454E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.02105302253943036 26-27 0.008849470320354616 28-29 0.005533287652127511 30-31 0.0017623142179721183 32-33 7.958838403745051E-4 34-35 0.002065508061924311 36-37 7.39034994633469E-4 38-39 4.926899964223126E-4 40-41 0.001610717295996022 42-43 9.285311471035892E-4 44-45 4.358411506812766E-4 46-47 0.0329344312993069 48-49 0.017679991025462218 50-51 0.0865049936026099 52-53 0.0018570622942071784 54-55 0.03651590858099217 56-57 8.337830708685291E-4 58-59 0.001478069989266938 60-61 0.003221434591992044 62-63 0.003031938439521924 64-65 0.009607454930235097 66-67 0.005116396116693247 68-69 0.0019328607551952265 70-71 0.005400640345398427 72-73 0.004528958044035874 74-75 9.285311471035892E-4 76-77 0.0 78-79 0.0 80-81 4.547907659282886E-4 82-83 5.684884574103607E-4 84-85 7.579846098804809E-5 86-87 0.0012317249910557817 88-89 0.003410930744462164 90-91 0.0011369769148207214 92-93 0.003031938439521924 94-95 6.253373031513968E-4 96-97 3.0319384395219236E-4 98-99 0.0017244149874780944 100-101 0.003069837670015948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2638576.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.37556921752412 #Duplication Level Percentage of deduplicated Percentage of total 1 83.0694929269159 56.79923863488969 2 10.904954933190508 14.912650016966978 3 2.880224031918269 5.90810872969212 4 1.0888727458337717 2.9780917520733046 5 0.5561072306535165 1.9012074220957589 6 0.3253933029473154 1.3349371385155773 7 0.2136917704939025 1.0227907511234777 8 0.15294088954182658 0.83659363032455 9 0.10927598093375514 0.672462665833394 >10 0.6261964635233656 7.914392207043311 >50 0.05112914240828134 2.3690483254536012 >100 0.02099551870136898 2.4155023533730957 >500 5.019666495403268E-4 0.22320906755268904 >1k 1.1154814433310025E-4 0.19967925467779918 >5k 1.1154814433310025E-4 0.5120880503845469 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7535 0.28557070177247124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5893 0.22334016530128373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3803 0.14413077356877346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.57984609880481E-5 2 0.0 0.0 0.0 0.0 7.57984609880481E-5 3 0.0 0.0 0.0 0.0 7.57984609880481E-5 4 0.0 0.0 0.0 0.0 7.57984609880481E-5 5 0.0 0.0 0.0 3.789923049402405E-5 7.57984609880481E-5 6 0.0 0.0 0.0 3.789923049402405E-5 7.57984609880481E-5 7 0.0 0.0 0.0 3.789923049402405E-5 1.1369769148207214E-4 8 0.0 0.0 0.0 3.789923049402405E-5 1.8949615247012026E-4 9 0.0 0.0 0.0 3.789923049402405E-5 1.8949615247012026E-4 10-11 7.57984609880481E-5 0.0 0.0 7.57984609880481E-5 1.8949615247012026E-4 12-13 7.57984609880481E-5 0.0 0.0 1.3264730672908417E-4 1.8949615247012026E-4 14-15 9.474807623506012E-5 0.0 0.0 1.515969219760962E-4 1.8949615247012026E-4 16-17 1.1369769148207214E-4 0.0 0.0 1.515969219760962E-4 1.8949615247012026E-4 18-19 1.1369769148207214E-4 0.0 0.0 1.515969219760962E-4 2.2739538296414428E-4 20-21 1.1369769148207214E-4 0.0 0.0 1.515969219760962E-4 2.6529461345816834E-4 22-23 1.1369769148207214E-4 0.0 0.0 2.463449982111563E-4 2.6529461345816834E-4 24-25 1.3264730672908417E-4 0.0 0.0 4.547907659282886E-4 2.6529461345816834E-4 26-27 1.7054653722310822E-4 0.0 0.0 9.095815318565771E-4 2.6529461345816834E-4 28-29 1.8949615247012026E-4 1.8949615247012026E-5 0.0 0.0025771476735936354 2.6529461345816834E-4 30-31 1.8949615247012026E-4 3.789923049402405E-5 0.0 0.007712493405533894 2.6529461345816834E-4 32-33 1.8949615247012026E-4 3.789923049402405E-5 0.0 0.020674030234490118 2.6529461345816834E-4 34-35 1.8949615247012026E-4 3.789923049402405E-5 0.0 0.043622014298621684 2.6529461345816834E-4 36-37 1.8949615247012026E-4 3.789923049402405E-5 0.0 0.07981577942041465 2.8424422870518035E-4 38-39 1.8949615247012026E-4 3.789923049402405E-5 0.0 0.1293879729065981 3.789923049402405E-4 40-41 2.2739538296414428E-4 3.789923049402405E-5 0.0 0.18019189138383734 3.789923049402405E-4 42-43 2.2739538296414428E-4 3.789923049402405E-5 0.0 0.2341414459920806 3.789923049402405E-4 44-45 2.463449982111563E-4 3.789923049402405E-5 0.0 0.30283380126249915 3.789923049402405E-4 46-47 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.3855109725852126 3.789923049402405E-4 48-49 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.4674301592980456 3.789923049402405E-4 50-51 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.5537077575176914 3.789923049402405E-4 52-53 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.6358922388439825 3.789923049402405E-4 54-55 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.7306592646942897 3.789923049402405E-4 56-57 2.6529461345816834E-4 3.789923049402405E-5 0.0 0.8426893900346247 3.789923049402405E-4 58-59 2.6529461345816834E-4 5.684884574103608E-5 0.0 0.9625836057024698 3.9794192018725253E-4 60-61 2.6529461345816834E-4 7.57984609880481E-5 0.0 1.0858887521147769 4.1689153543426454E-4 62-63 2.6529461345816834E-4 7.57984609880481E-5 0.0 1.2082653673799806 4.1689153543426454E-4 64-65 2.6529461345816834E-4 1.1369769148207214E-4 0.0 1.3401546894991845 4.1689153543426454E-4 66-67 2.8424422870518035E-4 1.1369769148207214E-4 0.0 1.4931538830035596 4.3584115068127656E-4 68-69 3.031938439521924E-4 1.1369769148207214E-4 0.0 1.662506594466106 4.926899964223126E-4 70-71 3.031938439521924E-4 1.1369769148207214E-4 0.0 1.8416183577808636 5.305892269163367E-4 72-73 3.031938439521924E-4 1.1369769148207214E-4 0.0 2.0333505648501315 5.684884574103607E-4 74-75 3.031938439521924E-4 1.1369769148207214E-4 0.0 2.231790935716841 5.684884574103607E-4 76-77 3.031938439521924E-4 1.1369769148207214E-4 0.0 2.44736175876685 5.684884574103607E-4 78-79 3.031938439521924E-4 1.1369769148207214E-4 0.0 2.684857286657652 5.874380726573728E-4 80-81 3.031938439521924E-4 1.1369769148207214E-4 0.0 2.942818399015226 6.253373031513969E-4 82-83 3.031938439521924E-4 1.1369769148207214E-4 0.0 3.2108607066842114 6.442869183984089E-4 84-85 3.031938439521924E-4 1.1369769148207214E-4 0.0 3.4761553201423796 6.821861488924329E-4 86-87 3.031938439521924E-4 1.1369769148207214E-4 0.0 3.75507470696315 7.200853793864569E-4 88-89 3.031938439521924E-4 1.1369769148207214E-4 0.0 4.05523661247582 7.76934225127493E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3560 0.0 54.529026 1 GTATCAA 6450 0.0 41.08782 1 ATCAACG 7850 0.0 32.729385 3 TCAACGC 8020 0.0 32.152832 4 CAACGCA 8010 0.0 32.01511 5 TATCAAC 8425 0.0 31.059319 2 AACGCAG 8460 0.0 30.423874 6 ACGCAGA 9715 0.0 26.053734 7 CGCAGAG 9660 0.0 25.759636 8 GTGGTAT 1705 0.0 23.440804 1 GCAGAGT 10880 0.0 22.434675 9 GAGTACT 7220 0.0 21.113167 12-13 GTACATG 6815 0.0 21.084274 1 TGGTATC 1875 0.0 20.009493 2 ACATGGG 6790 0.0 19.933582 3 AGAGTAC 10335 0.0 19.89587 10-11 TACATGG 7135 0.0 19.701895 2 CAGAGTA 11020 0.0 19.650284 10-11 GTACTTT 7980 0.0 19.340425 14-15 AGTACTT 8145 0.0 17.636803 12-13 >>END_MODULE