FastQCFastQC Report
Sat 18 Jun 2016
SRR1931134_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931134_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874654
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44710.2384973440432208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.18254035144618685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.10369913594722013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.048.2665631
GTATCAA44450.040.8022271
ATCAACG52800.033.622023
TATCAAC54600.033.0414472
CAACGCA54750.032.3316845
TCAACGC55650.031.9087034
AACGCAG57050.030.9224646
ACGCAGA62650.027.7893547
CGCAGAG63500.027.191918
GTACATG49500.023.7478731
TACATGG48250.022.9709242
GCAGAGT70400.022.90589
ACATGGG47850.022.4396133
GAGTACT42300.021.3044912-13
GGCGTCG3151.4551915E-1121.140948
CAGAGTA67150.020.5433110-11
AGAGTAC62450.019.84237910-11
GTGGTAT13200.019.6283441
TATACCG2502.1333653E-719.0339765
GTACTTT49200.018.7843814-15
Produced by FastQC (version 0.11.5)