FastQCFastQC Report
Sat 18 Jun 2016
SRR1931134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874654
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58800.31365788033418435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45780.24420506397447211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.1628033759829814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.051.5617071
GTATCAA52200.042.6424181
ATCAACG65200.033.8669663
CAACGCA66400.033.1833955
TCAACGC67650.032.710644
TATCAAC68550.032.3504522
AACGCAG70200.031.7253686
ACGCAGA79450.027.6731137
CGCAGAG79450.027.1351918
CGACGGT1151.2846362E-424.775617
GCAGAGT89500.023.3984179
GTACATG51300.022.9005581
TGGTATC14200.022.4056422
TACATGG52100.022.2393992
GTGGTAT15250.022.1439461
ACATGGG52700.021.6257693
ACGACGG1100.002882757721.584816
GAGTACT56800.021.23519712-13
AGAGTAC81050.020.82847610-11
CAGAGTA87650.019.77479210-11
Produced by FastQC (version 0.11.5)