FastQCFastQC Report
Sat 18 Jun 2016
SRR1931132_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931132_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610212
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52370.20063504420330608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38880.14895341834303114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31050.052.4746671
GTATCAA58600.044.0441551
ATCAACG67200.037.5929533
TCAACGC68950.036.7195744
CAACGCA68550.036.4620135
AACGCAG72100.035.5089766
TATCAAC76350.033.931242
ACGCAGA80600.031.1189697
CGCAGAG80250.030.6599048
GCAGAGT90500.025.7652729
CCGATCG3050.023.4512469
GAGTACT58050.022.02746412-13
GTACATG61000.021.273821
AGAGTAC81100.020.93125310-11
CAGAGTA89800.020.81497210-11
TACATGG60900.020.519942
GTACTAG7500.019.8660721
ACATGGG60100.019.5044383
TCGACCG1507.526153E-419.073689
GTACTTT68150.018.94067614-15
Produced by FastQC (version 0.11.5)