FastQCFastQC Report
Sat 18 Jun 2016
SRR1931132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610212
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67250.257641908013602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55160.21132383116773656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36100.1383029424429893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.051.957541
GTATCAA64200.040.5982131
ATCAACG75950.033.0774153
TCAACGC78750.031.9001054
CAACGCA78650.031.8802835
TATCAAC82500.030.9693432
AACGCAG82300.030.350416
ACGCAGA93800.026.2244037
CGCAGAG94550.025.6145868
GTGGTAT18750.022.3224981
GCAGAGT106250.022.1682519
TGGTATC17700.021.7327882
TAGCGCG1100.002882437521.585614
GAGTACT68600.021.04399312-13
GTACATG63250.020.52881
AGAGTAC97500.019.84731110-11
CAGAGTA106150.019.77338410-11
TACATGG67300.018.8408222
ACATGGG64300.018.833583
TCTAACG2302.06398E-618.5831092
Produced by FastQC (version 0.11.5)