FastQCFastQC Report
Sat 18 Jun 2016
SRR1931131_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931131_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236953
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54270.2426067959407283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40820.18248036503225593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23660.10576887399958784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30150.054.390041
GTATCAA56900.043.4401051
ATCAACG67550.034.6807823
CAACGCA69850.033.7059445
TATCAAC71800.033.427332
TCAACGC70250.033.3561064
AACGCAG73950.032.7821126
ACGCAGA80900.028.7978137
CGCAGAG81650.028.5332938
GTGGTAT13100.027.0067941
GTACTAG7250.025.7181191
GCAGAGT88600.024.9534849
GTACATG60800.024.8482651
CTAGCGG1000.001611693623.8562833
TACATGG60800.023.1188432
ACATGGG60150.021.972373
CAGAGTA85750.021.42977510-11
AGAGTAC81450.021.36447710-11
GTATAAG11950.021.2041341
GAGTACT56300.020.94613612-13
Produced by FastQC (version 0.11.5)