##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931131_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2236953 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.849850667403384 31.0 28.0 31.0 23.0 34.0 2 28.57336832736316 31.0 28.0 33.0 18.0 34.0 3 28.589173308513857 31.0 28.0 33.0 16.0 34.0 4 31.79768283017122 35.0 32.0 37.0 19.0 37.0 5 31.88004039423269 35.0 32.0 37.0 19.0 37.0 6 32.15819912175178 35.0 32.0 37.0 23.0 37.0 7 31.86652424078646 35.0 32.0 37.0 19.0 37.0 8 32.026515085475644 35.0 32.0 37.0 22.0 37.0 9 33.04158782057558 37.0 32.0 39.0 18.0 39.0 10-11 33.034343144446936 37.0 32.0 39.0 20.0 39.0 12-13 33.10650693152695 37.0 32.0 39.0 20.5 39.0 14-15 33.82560228131749 38.0 32.0 40.0 18.5 41.0 16-17 33.79900002369294 38.0 32.0 40.0 19.0 41.0 18-19 33.83345045693852 38.0 32.0 40.0 19.0 41.0 20-21 34.05613171130551 38.0 32.0 40.0 22.5 41.0 22-23 33.952081916785914 38.0 32.0 40.0 22.0 41.0 24-25 33.84324190092505 38.0 32.0 40.0 21.0 41.0 26-27 33.47050899147188 38.0 31.5 40.0 18.0 41.0 28-29 33.415307339939645 37.5 31.5 40.0 18.0 41.0 30-31 33.17782313709765 37.0 31.0 40.0 17.5 41.0 32-33 32.95848057603356 37.0 31.0 40.0 16.5 41.0 34-35 32.91636927552791 37.0 31.0 40.0 17.0 41.0 36-37 32.56631833570039 37.0 30.0 40.0 15.5 41.0 38-39 32.38925851370145 37.0 30.0 40.0 15.0 41.0 40-41 32.232026332247486 36.5 30.0 40.0 12.5 41.0 42-43 32.43244381978522 37.0 30.0 40.0 13.0 41.0 44-45 32.49186303869594 37.0 30.0 40.0 12.0 41.0 46-47 32.26286672093692 37.0 30.0 40.0 9.0 41.0 48-49 32.027987847755405 37.0 30.0 40.0 8.5 41.0 50-51 31.494786658459073 36.0 29.5 39.5 8.0 40.5 52-53 31.43509474718512 36.0 30.0 39.5 7.0 40.5 54-55 31.437394974324448 36.0 30.0 40.0 2.0 41.0 56-57 31.195585468268668 36.0 29.0 40.0 2.0 41.0 58-59 31.046935496633147 35.5 29.0 40.0 2.0 41.0 60-61 30.892035952476427 35.0 29.0 39.0 2.0 41.0 62-63 30.731228371807543 35.0 29.0 39.0 2.0 41.0 64-65 30.372873726001394 35.0 28.5 39.0 2.0 40.0 66-67 30.164121910473753 35.0 28.5 39.0 2.0 40.0 68-69 29.954566099511254 34.0 28.0 38.0 2.0 40.0 70-71 29.661312508577517 34.0 28.0 38.0 2.0 40.0 72-73 29.33743690636325 34.0 28.0 37.0 2.0 40.0 74-75 28.91506079922108 34.0 28.0 37.0 2.0 39.0 76-77 28.601506602955002 34.0 27.5 36.0 2.0 39.0 78-79 28.189896926757065 33.5 26.5 36.0 2.0 39.0 80-81 27.796951925230438 33.0 26.0 35.5 2.0 37.5 82-83 27.458839769990696 33.0 26.0 35.0 2.0 37.0 84-85 27.12970209923946 33.0 26.0 35.0 2.0 36.5 86-87 26.814077005641156 33.0 25.5 35.0 2.0 36.0 88-89 26.47715486199308 32.5 25.0 35.0 2.0 36.0 90-91 26.094891801481747 32.0 24.5 35.0 2.0 35.5 92-93 25.742518059163515 32.0 23.5 35.0 2.0 35.0 94-95 25.440192529749172 32.0 21.5 35.0 2.0 35.0 96-97 25.051933366503455 32.0 19.5 35.0 2.0 35.0 98-99 24.689567237219556 32.0 16.5 34.0 2.0 35.0 100-101 24.097035789308045 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.30265336060064 1101 2 5.886613130871076 1101 3 5.512748675815679 1101 4 6.869008404147266 1101 5 6.278507409953594 1101 6 5.791701437504148 1101 7 6.351972105422565 1101 8 5.876046287551553 1101 9 6.554997582285139 1101 10-11 6.481516485685148 1101 12-13 6.3885921147507965 1101 14-15 6.694582033669338 1101 16-17 6.616338348665135 1101 18-19 6.667218908411222 1101 20-21 6.050011891696059 1101 22-23 6.048354471189835 1101 24-25 5.931726364379152 1101 26-27 6.072120657457464 1101 28-29 6.082832891520724 1101 30-31 6.219246599109635 1101 32-33 5.955825614955359 1101 34-35 5.767943632247132 1101 36-37 5.978802111773039 1101 38-39 5.861688648129899 1101 40-41 6.1578181139341766 1101 42-43 5.954228790900974 1101 44-45 5.954977640843673 1101 46-47 5.838312970496133 1101 48-49 5.6516478668703485 1101 50-51 5.585111066898616 1101 52-53 5.341202988517413 1101 54-55 5.736972845800949 1101 56-57 5.656776462523382 1101 58-59 5.546736648931343 1101 60-61 5.388694144439317 1101 62-63 5.386784178370867 1101 64-65 5.458973684133184 1101 66-67 5.378099764019463 1101 68-69 5.457965199044029 1101 70-71 5.251886889515635 1101 72-73 4.98285586231578 1101 74-75 5.213296423452626 1101 76-77 5.112169315053638 1101 78-79 5.034739274624631 1101 80-81 4.946085678564614 1101 82-83 4.920605763254564 1101 84-85 4.826623763369149 1101 86-87 4.780813637916346 1101 88-89 4.7946771896196445 1101 90-91 4.6354069899962305 1101 92-93 4.6728168974222974 1101 94-95 4.438597519619741 1101 96-97 4.439061158969672 1101 98-99 4.328058380836733 1101 100-101 4.196650191025135 1102 1 5.347815445859329 1102 2 5.883567004808885 1102 3 5.590136672266368 1102 4 6.921728686891598 1102 5 6.519763724166079 1102 6 5.966889380615076 1102 7 6.487498192835883 1102 8 6.093032563018944 1102 9 6.763621074022673 1102 10-11 6.760900143373732 1102 12-13 6.768292007417042 1102 14-15 7.023747508914656 1102 16-17 6.932109361521459 1102 18-19 6.953047947175676 1102 20-21 6.309317806495351 1102 22-23 6.443420877135676 1102 24-25 6.177641601115695 1102 26-27 6.434061027764695 1102 28-29 6.308169122493425 1102 30-31 6.579969410553591 1102 32-33 6.285467915307457 1102 34-35 6.392800100449612 1102 36-37 6.490852496934071 1102 38-39 6.386218426351704 1102 40-41 6.455023703294245 1102 42-43 6.432177072887416 1102 44-45 6.320338400664546 1102 46-47 6.375150532204707 1102 48-49 6.170281568591804 1102 50-51 6.223175090258305 1102 52-53 5.929762155688554 1102 54-55 6.267015489385024 1102 56-57 6.177099673489565 1102 58-59 6.280524787724005 1102 60-61 6.1071930279347875 1102 62-63 5.947321179396226 1102 64-65 6.056171377721043 1102 66-67 6.029257331690591 1102 68-69 6.049832604975617 1102 70-71 5.825961483845482 1102 72-73 5.594833049627489 1102 74-75 5.804920381752684 1102 76-77 5.670041316568394 1102 78-79 5.5803984856554685 1102 80-81 5.599757482037241 1102 82-83 5.4188490982542845 1102 84-85 5.372787316048445 1102 86-87 5.3751452175031 1102 88-89 5.3608451398143195 1102 90-91 5.392173269109403 1102 92-93 5.346102094125804 1102 94-95 5.348018516421536 1102 96-97 5.200221361558128 1102 98-99 4.8964998451990915 1102 100-101 4.707175877556434 1103 1 4.952545492559505 1103 2 5.643876451873389 1103 3 5.307844095407585 1103 4 6.764493451740272 1103 5 6.27548399868709 1103 6 5.605620669935217 1103 7 6.295653699874588 1103 8 5.669969085701318 1103 9 6.21561288088504 1103 10-11 6.372357323568437 1103 12-13 6.442428469475164 1103 14-15 6.732189885927372 1103 16-17 6.523762998236055 1103 18-19 6.351675342121801 1103 20-21 5.653934472670322 1103 22-23 6.067999194797398 1103 24-25 5.720895418082357 1103 26-27 6.024979873943817 1103 28-29 5.782816110925303 1103 30-31 6.028971503407217 1103 32-33 5.848281506232254 1103 34-35 5.817640205242974 1103 36-37 5.814690035072719 1103 38-39 5.728116560636451 1103 40-41 5.967675028107678 1103 42-43 5.824863658023663 1103 44-45 5.597216356619278 1103 46-47 5.64812038885772 1103 48-49 5.516405737651432 1103 50-51 5.374958225851639 1103 52-53 5.039353095173315 1103 54-55 5.452131392755426 1103 56-57 5.601802335448944 1103 58-59 5.657548049433018 1103 60-61 5.443472497900526 1103 62-63 5.3157143162949865 1103 64-65 5.308221941236656 1103 66-67 5.495361529439112 1103 68-69 5.497357804622258 1103 70-71 5.259830462269811 1103 72-73 5.155985836045122 1103 74-75 5.258746205279628 1103 76-77 5.24356668031076 1103 78-79 5.236748034373221 1103 80-81 5.173341342867854 1103 82-83 5.184861188412224 1103 84-85 5.101774896053023 1103 86-87 5.070041846690689 1103 88-89 4.961499414552353 1103 90-91 4.997277121066691 1103 92-93 4.912486856318047 1103 94-95 4.970730150213555 1103 96-97 4.80814972389669 1103 98-99 4.578887498818837 1103 100-101 4.515793188718781 1104 1 4.7068349697045875 1104 2 5.488555126744977 1104 3 5.006740632000948 1104 4 6.562194652747749 1104 5 6.152325228727168 1104 6 5.827995409947544 1104 7 6.330536456887639 1104 8 5.855131043139693 1104 9 6.520683115732517 1104 10-11 6.490037700630808 1104 12-13 6.387048804889648 1104 14-15 6.535000308246456 1104 16-17 6.412301569904141 1104 18-19 6.578234255312573 1104 20-21 5.8814701977967445 1104 22-23 6.122305469467786 1104 24-25 5.7657558116368435 1104 26-27 6.0735580678265215 1104 28-29 6.095986025217414 1104 30-31 6.322507697243804 1104 32-33 6.265863996417082 1104 34-35 6.108341730981827 1104 36-37 6.156233774395066 1104 38-39 5.941775660002385 1104 40-41 6.249999169860089 1104 42-43 6.269276938228199 1104 44-45 6.0961195935242145 1104 46-47 6.08068555484158 1104 48-49 5.9547892360617425 1104 50-51 5.950524558001359 1104 52-53 5.607753241520587 1104 54-55 5.953409905697626 1104 56-57 5.883973024484057 1104 58-59 5.789872315842388 1104 60-61 5.580826797287894 1104 62-63 5.566864370240282 1104 64-65 5.736677104933058 1104 66-67 5.718680203113994 1104 68-69 5.689292125363366 1104 70-71 5.616694915901469 1104 72-73 5.52175431815342 1104 74-75 5.58309697625176 1104 76-77 5.491941091228718 1104 78-79 5.573787107473933 1104 80-81 5.459209637803728 1104 82-83 5.319340368518471 1104 84-85 5.290846500880491 1104 86-87 5.247175677779264 1104 88-89 5.340133741463983 1104 90-91 5.1903178658172315 1104 92-93 5.24737279208356 1104 94-95 5.048137946135153 1104 96-97 4.9705674892586345 1104 98-99 4.805470720116727 1104 100-101 4.82236482315227 1105 1 4.515690291736618 1105 2 5.151452400469623 1105 3 4.904900989405434 1105 4 6.38165173635192 1105 5 3.6105327723279714 1105 6 4.966014346332162 1105 7 5.648063102299876 1105 8 1.324367500323035 1105 9 0.7334729136091411 1105 10-11 2.1457039993001708 1105 12-13 5.463268268851696 1105 14-15 3.6676024618904854 1105 16-17 5.782920596396512 1105 18-19 6.325897128511706 1105 20-21 3.459826109656227 1105 22-23 5.468216400363399 1105 24-25 5.592209315005569 1105 26-27 5.772190631116896 1105 28-29 3.387324675595899 1105 30-31 5.583947082585123 1105 32-33 5.747518814946261 1105 34-35 5.755305409304036 1105 36-37 5.822280348408956 1105 38-39 5.655501959428378 1105 40-41 5.939928081035177 1105 42-43 2.9981918964587813 1105 44-45 5.25727627439489 1105 46-47 5.58061470676256 1105 48-49 5.617246976558299 1105 50-51 5.649918566179839 1105 52-53 5.609775850999785 1105 54-55 5.802149256990603 1105 56-57 5.7437299399100255 1105 58-59 5.639785475357186 1105 60-61 5.499436111402833 1105 62-63 5.5105594070916055 1105 64-65 5.657198285413379 1105 66-67 5.740131006689413 1105 68-69 5.647969811313779 1105 70-71 5.503613471563639 1105 72-73 5.461050927419386 1105 74-75 5.5973917799234805 1105 76-77 5.365850770245647 1105 78-79 5.405208230519165 1105 80-81 5.3980010246032855 1105 82-83 5.286286644351364 1105 84-85 5.115176625414907 1105 86-87 5.194196791194656 1105 88-89 5.264873821873113 1105 90-91 5.353330697573682 1105 92-93 5.295630307654662 1105 94-95 5.22936698834479 1105 96-97 5.206389103425497 1105 98-99 5.187160274839993 1105 100-101 5.273838360019067 1106 1 4.382344456086045 1106 2 4.632544154544732 1106 3 4.735102362935873 1106 4 6.333155247778606 1106 5 6.212554871251481 1106 6 5.472533399432518 1106 7 6.0499301724896135 1106 8 5.681963347829253 1106 9 6.200589922365076 1106 10-11 6.033151414122742 1106 12-13 6.360520345745261 1106 14-15 6.1629273712486885 1106 16-17 6.142276425278585 1106 18-19 6.291678717340666 1106 20-21 5.587017313198267 1106 22-23 5.652636745513579 1106 24-25 5.770934725318689 1106 26-27 5.970017599046297 1106 28-29 5.908999511880218 1106 30-31 6.138346173795991 1106 32-33 5.939281555454201 1106 34-35 5.397184550696615 1106 36-37 5.610464753277103 1106 38-39 5.714972895851943 1106 40-41 5.780614070248689 1106 42-43 2.5131975656129804 1106 44-45 4.7708793128891 1106 46-47 5.090968651674757 1106 48-49 4.936269252238702 1106 50-51 5.1409528958185255 1106 52-53 4.9352176785798925 1106 54-55 5.379856073340186 1106 56-57 5.09157303151752 1106 58-59 5.015480798197196 1106 60-61 5.0040104937236904 1106 62-63 4.832846856920668 1106 64-65 5.072896970945614 1106 66-67 5.110552334780397 1106 68-69 5.035473659009686 1106 70-71 5.041214794222583 1106 72-73 4.8506797810431195 1106 74-75 4.996584518232737 1106 76-77 4.841797432773614 1106 78-79 4.980753223772222 1106 80-81 4.772791955681029 1106 82-83 4.885040637548379 1106 84-85 4.846053900082193 1106 86-87 4.896372280617264 1106 88-89 4.887499006713405 1106 90-91 4.874023972620346 1106 92-93 4.905300030922334 1106 94-95 4.815183540550585 1106 96-97 4.829647336632515 1106 98-99 4.619424228934932 1106 100-101 4.63245362060616 1107 1 3.216446105455951 1107 2 -0.08574601409870652 1107 3 1.1252413547816822 1107 4 2.793140653634797 1107 5 2.754153740784467 1107 6 4.199556657401384 1107 7 5.061428967893615 1107 8 4.6168887555386675 1107 9 4.94632889567864 1107 10-11 2.900851794033791 1107 12-13 5.1031167134250275 1107 14-15 3.7675669190618812 1107 16-17 2.1144890596373678 1107 18-19 2.767973078904788 1107 20-21 2.4113898808409644 1107 22-23 4.421492495142932 1107 24-25 4.512872850781996 1107 26-27 3.5809498868520784 1107 28-29 4.273123515771985 1107 30-31 4.004303016827873 1107 32-33 4.4129329870702065 1107 34-35 4.893967121119154 1107 36-37 4.957918654314906 1107 38-39 4.931729521129924 1107 40-41 3.6262636254747846 1107 42-43 4.268344202010315 1107 44-45 4.628732815483609 1107 46-47 4.821875689295226 1107 48-49 2.8200998675092777 1107 50-51 4.115021650451585 1107 52-53 4.3362817769861195 1107 54-55 4.924820282939891 1107 56-57 3.487427947195801 1107 58-59 2.7257936456768093 1107 60-61 3.670917925154381 1107 62-63 4.406911094318744 1107 64-65 4.619140021353324 1107 66-67 4.797741224698854 1107 68-69 4.722278236474477 1107 70-71 4.404823601672547 1107 72-73 4.310164005468618 1107 74-75 4.465610764526286 1107 76-77 4.33896049337406 1107 78-79 4.4152027002362715 1107 80-81 4.464076703642483 1107 82-83 4.300103415532071 1107 84-85 4.364652167317182 1107 86-87 4.370676626428978 1107 88-89 4.272179672947654 1107 90-91 4.36909439515556 1107 92-93 4.300810825955693 1107 94-95 4.422285444738872 1107 96-97 4.5009862914138346 1107 98-99 4.313332911292232 1107 100-101 4.384514058893778 1108 1 -1.9025046463502377 1108 2 -1.1170556735989443 1108 3 -1.7829531268271097 1108 4 -3.574377787743874 1108 5 -8.352406488616765 1108 6 -6.391212297274116 1108 7 -5.100832329782527 1108 8 -5.549740067815179 1108 9 -7.427987030159617 1108 10-11 -3.0834268824065276 1108 12-13 -1.5926757963006004 1108 14-15 -6.135987950954377 1108 16-17 -5.641460568447556 1108 18-19 -3.8319851272835876 1108 20-21 -4.382022054017938 1108 22-23 -3.2702421233192887 1108 24-25 -7.773295198612857 1108 26-27 -8.635888034205696 1108 28-29 -5.727649565218279 1108 30-31 -5.3392532908054555 1108 32-33 -2.2275893374826197 1108 34-35 -0.7399758540470565 1108 36-37 -4.0984904484062135 1108 38-39 -5.096834514901197 1108 40-41 -2.7512674810851223 1108 42-43 -3.428696654616491 1108 44-45 -1.162769934964075 1108 46-47 -1.9306294468892489 1108 48-49 -0.8618323412880429 1108 50-51 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-4.366569286754739 2313 46-47 -5.3213463879885055 2313 48-49 -3.519850223234826 2313 50-51 -3.6647537578968716 2313 52-53 -3.0066813015075304 2313 54-55 -4.202795699365012 2313 56-57 -3.032037135230091 2313 58-59 -2.6976906442505957 2313 60-61 -1.246703445081554 2313 62-63 -3.8391023093412713 2313 64-65 -5.373099467454907 2313 66-67 -5.487880561447547 2313 68-69 -6.647872625594488 2313 70-71 -6.516789091158671 2313 72-73 -6.50540569734445 2313 74-75 -7.243103032358167 2313 76-77 -5.898247896674338 2313 78-79 -6.708188009836327 2313 80-81 -8.286651139672703 2313 82-83 -7.410002263536288 2313 84-85 -6.584025426451724 2313 86-87 -5.695779249251604 2313 88-89 -7.785071820549019 2313 90-91 -7.280330892200762 2313 92-93 -5.83442151675453 2313 94-95 -5.731400561764454 2313 96-97 -6.146264918507521 2313 98-99 -5.030591960153625 2313 100-101 -4.332338349605918 2314 1 -12.04823904491999 2314 2 -14.33503273536616 2314 3 -12.777887001824118 2314 4 -25.02881658320731 2314 5 -18.856295373071603 2314 6 -17.67868246719582 2314 7 -17.03223711696138 2314 8 -12.63326229172234 2314 9 -15.412106254697301 2314 10-11 -13.774287505106917 2314 12-13 -14.784974049195812 2314 14-15 -17.02610252239891 2314 16-17 -17.62678796103932 2314 18-19 -15.455547497088766 2314 20-21 -16.53225690353068 2314 22-23 -16.34444718665084 2314 24-25 -16.92967464519008 2314 26-27 -15.446502952836424 2314 28-29 -17.337690628964857 2314 30-31 -18.29011746518072 2314 32-33 -15.561938622757934 2314 34-35 -16.63329690949309 2314 36-37 -16.01186350196866 2314 38-39 -15.188311093800257 2314 40-41 -17.46245146582489 2314 42-43 -17.430425601963908 2314 44-45 -14.765349488490607 2314 46-47 -13.742206111188132 2314 48-49 -13.666138048709843 2314 50-51 -13.02330407457527 2314 52-53 -12.826702413400561 2314 54-55 -13.158108859111667 2314 56-57 -12.139766433846088 2314 58-59 -11.311343001745318 2314 60-61 -11.734476140366567 2314 62-63 -14.836170101975565 2314 64-65 -12.82307600979598 2314 66-67 -13.88827347723455 2314 68-69 -16.78888247781124 2314 70-71 -16.694041026415533 2314 72-73 -15.5784176607505 2314 74-75 -13.834969089125702 2314 76-77 -14.174520474671054 2314 78-79 -13.757800958464053 2314 80-81 -13.588873752855907 2314 82-83 -14.026704116691345 2314 84-85 -14.119798356313321 2314 86-87 -14.386665948758994 2314 88-89 -13.62459211142399 2314 90-91 -13.266109686477094 2314 92-93 -13.420481802937967 2314 94-95 -13.453104760685402 2314 96-97 -13.197959734364899 2314 98-99 -13.39201584005041 2314 100-101 -12.841868023544459 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68172.0 3 11473.0 4 8204.0 5 6607.0 6 6863.0 7 8070.0 8 9614.0 9 11036.0 10 12155.0 11 13164.0 12 14659.0 13 15712.0 14 17488.0 15 19449.0 16 20820.0 17 22225.0 18 20351.0 19 18355.0 20 18359.0 21 19911.0 22 22878.0 23 27918.0 24 34151.0 25 41870.0 26 50041.0 27 57326.0 28 63994.0 29 71337.0 30 81482.0 31 91382.0 32 104546.0 33 125605.0 34 151826.0 35 184036.0 36 224321.0 37 267113.0 38 235957.0 39 58421.0 40 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21499691003876 20.267641621733294 14.392690698903623 23.124670769324332 2 14.773925462014253 20.595567190524054 41.235452617352585 23.39505473010911 3 17.894609319015643 25.96362105059874 30.848256534670153 25.293513095715465 4 11.534514397249417 17.564042193349643 40.110866279289205 30.79057713011174 5 12.502671252484769 37.67194017828062 35.794693694141706 14.030694875092905 6 29.49050710362074 38.10084801388107 18.78118306728406 13.627461815214136 7 25.824292855979426 32.88235357290524 23.180031538005803 18.113322033109537 8 24.636483969674707 35.571096280398905 21.25509352029935 18.537326229627038 9 25.220595638428545 16.315284294181215 21.870906971488917 36.59321309590132 10-11 23.55086734903973 26.866313305558588 29.402228305531736 20.180591039869945 12-13 24.604143364022846 24.518803903983592 29.17933266826328 21.69772006373028 14-15 21.922967099068003 26.009379039216878 27.129998782640453 24.937655079074663 16-17 21.168983558518544 29.041933184189432 27.808273861494875 21.98080939579715 18-19 20.884443215615775 28.092142747603692 29.944669128552764 21.07874490822777 20-21 21.99045594572566 27.20643889180862 29.889795236206286 20.913309926259437 22-23 21.700241931622884 27.124530577928873 29.643469658320242 21.531757832128 24-25 21.401634677443713 27.288240814587038 29.755648496251112 21.554476011718133 26-27 21.41607906279496 27.476152566263266 29.61722764403832 21.49054072690345 28-29 21.253571822523913 27.521923175973633 29.75877738117586 21.465727620326593 30-31 21.592357001464816 27.270542438253305 29.705994107619414 21.43110645266247 32-33 21.459055593041963 27.508027262265067 29.51318726162074 21.51972988307223 34-35 21.705142159341204 27.56976473782255 29.489991850774043 21.235101252062204 36-37 21.63740007085483 27.251694215976535 29.44517266221023 21.66573305095841 38-39 21.514642967519677 27.55670355261176 29.51335124704065 21.41530223282792 40-41 21.564608376922585 27.296596794092416 29.57470106551835 21.56409376346665 42-43 21.519038603440478 27.356517443345986 29.409678670848493 21.714765282365043 44-45 21.572198856100258 27.63146267896783 29.1785706376099 21.61776782732201 46-47 21.73183847634115 27.564393673315163 28.908999879644266 21.794767970699418 48-49 21.79792439284721 27.66541064148561 28.858161027901836 21.678503937765342 50-51 21.537026473844914 27.78295249918912 28.743504568891275 21.93651645807469 52-53 21.548047608693125 28.015967399445387 28.571121771554512 21.864863220306983 54-55 21.578536682028414 27.840247688844922 28.58486541714889 21.99635021197777 56-57 21.640542535341115 27.90634324041465 28.392101418666783 22.061012805577455 58-59 21.632904656975573 27.94970151559624 28.606719245724545 21.810674581703644 60-61 21.800923783703073 28.01135779817746 28.401703759270912 21.786014658848554 62-63 21.632081675386118 27.961919629066863 28.433386843621662 21.972611851925365 64-65 21.575553889598932 27.75230413871011 28.56539676962368 22.106745202067277 66-67 21.64117887143807 27.85261916544514 28.36727012145539 22.138931841661403 68-69 21.65832272738855 27.81560452991189 28.388817288517014 22.13725545418254 70-71 21.6855696118783 27.62845263177188 28.38419045907536 22.301787297274462 72-73 21.739817510694234 27.748124345929487 28.356541241590683 22.1555169017856 74-75 21.610892137653316 27.838224584959985 28.341029069452954 22.209854207933738 76-77 21.72068434160217 27.93389043042031 28.12861959996477 22.216805628012747 78-79 21.648443217179796 27.927900139162514 28.12752436014525 22.29613228351244 80-81 21.673700788527967 27.999224838429775 28.13630863053448 22.19076574250778 82-83 21.843753534383602 27.8489981684908 28.27564995777739 22.031598339348214 84-85 22.01841075784784 27.798103044632587 27.93514213307119 22.248344064448382 86-87 21.72325480240309 27.893567723595442 28.130385394775843 22.252792079225625 88-89 21.953277516335838 27.699151479713702 28.05953008400266 22.288040919947804 90-91 21.948605983228077 27.705164122804547 28.13170415292588 22.214525741041495 92-93 21.932043274936934 27.72626425320514 28.054925606394054 22.286766865463868 94-95 21.76883018999505 27.687729693024394 28.13494516871834 22.408494948262213 96-97 21.787851599921858 27.640053233125595 28.138454406507424 22.433640760445122 98-99 21.800838014924768 27.638198030982323 27.983645610792895 22.577318343300014 100-101 21.884612685201702 27.57476352878223 27.94658627159355 22.59403751442252 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 251.0 1 178.5 2 141.0 3 170.5 4 153.0 5 174.0 6 283.5 7 536.5 8 889.5 9 1203.0 10 1236.5 11 1200.0 12 1333.5 13 1460.0 14 1687.0 15 1910.5 16 2084.0 17 2264.0 18 2526.5 19 2912.5 20 3346.0 21 3968.0 22 4851.0 23 6245.0 24 8055.0 25 10314.5 26 13433.0 27 17246.0 28 21667.0 29 27050.5 30 32775.5 31 38501.5 32 44913.0 33 51369.0 34 57612.0 35 62266.0 36 67032.0 37 73237.0 38 77044.5 39 78741.5 40 79535.0 41 79280.5 42 79310.0 43 80382.5 44 81524.0 45 82439.0 46 82645.5 47 82029.0 48 81723.5 49 80407.5 50 77314.0 51 74231.5 52 71679.5 53 68149.5 54 63595.5 55 57886.5 56 51621.5 57 45291.5 58 38906.0 59 33433.0 60 27689.5 61 22017.5 62 17482.5 63 13821.0 64 10723.0 65 8071.0 66 5935.0 67 4434.5 68 3308.0 69 2447.5 70 1882.0 71 1364.0 72 995.0 73 787.0 74 611.5 75 434.0 76 337.0 77 255.0 78 187.5 79 148.5 80 110.0 81 81.0 82 68.0 83 51.0 84 36.0 85 32.5 86 26.5 87 25.5 88 20.0 89 10.5 90 9.0 91 9.5 92 6.0 93 4.0 94 4.0 95 2.0 96 1.0 97 0.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03035378928390538 2 0.23987987230844812 3 0.0 4 0.5091300532465367 5 0.216991595263736 6 0.08788740755840646 7 0.09785632509936508 8 0.1006279523977482 9 0.09870569475532119 10-11 0.12467852476113714 12-13 0.18353090118567533 14-15 0.11663186486260552 16-17 0.10827227930135322 18-19 0.15961443982059526 20-21 0.096492863283225 22-23 0.09048022019237775 24-25 0.08741801906432545 26-27 0.16044145764349987 28-29 0.1016784885511676 30-31 0.11363671923370763 32-33 0.09249188516701065 34-35 0.13404394280970588 36-37 0.12550554258404178 38-39 0.12175043463139368 40-41 0.10129850738929248 42-43 0.09852688009090937 44-45 0.08402054044050099 46-47 0.08547340958884697 48-49 0.08907205471013473 50-51 0.07724793502590353 52-53 0.08489226192950858 54-55 0.2866622588851889 56-57 0.3053707431492749 58-59 0.3175971958284327 60-61 0.15295359357125518 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2236953.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.69637788310189 #Duplication Level Percentage of deduplicated Percentage of total 1 84.55787702110464 59.77935626876872 2 9.827312829505686 13.895108427403786 3 2.615972325395087 5.548193041436035 4 1.014522374939861 2.8689222875844163 5 0.5165549173168243 1.8259280816002335 6 0.31114903024168333 1.3198265651956045 7 0.22145455385360036 1.0959224376217511 8 0.15787732996215498 0.8929084302543738 9 0.10834476873480103 0.6893624440919499 >10 0.6186309579390751 7.936627014416478 >50 0.03596861332789633 1.7426600779006511 >100 0.013558055110013243 1.5867062446957205 >500 5.829169264649777E-4 0.27473410205216114 >1k 1.295370947699896E-4 0.29524758167204496 >5k 6.47685473849948E-5 0.2484969953061706 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5427 0.2426067959407283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4082 0.18248036503225593 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2366 0.10576887399958784 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.470366610295344E-5 0.0 7 0.0 0.0 0.0 4.470366610295344E-5 0.0 8 0.0 0.0 0.0 4.470366610295344E-5 0.0 9 0.0 0.0 0.0 4.470366610295344E-5 0.0 10-11 0.0 0.0 0.0 4.470366610295344E-5 0.0 12-13 0.0 0.0 0.0 4.470366610295344E-5 0.0 14-15 2.235183305147672E-5 0.0 0.0 4.470366610295344E-5 0.0 16-17 8.940733220590687E-5 0.0 0.0 6.705549915443015E-5 0.0 18-19 8.940733220590687E-5 0.0 0.0 8.940733220590687E-5 0.0 20-21 8.940733220590687E-5 0.0 0.0 1.341109983088603E-4 0.0 22-23 8.940733220590687E-5 0.0 0.0 2.682219966177206E-4 0.0 24-25 8.940733220590687E-5 0.0 0.0 4.917403271324878E-4 0.0 26-27 8.940733220590687E-5 0.0 0.0 9.61128821213499E-4 4.470366610295344E-5 28-29 8.940733220590687E-5 0.0 0.0 0.0021681278059932415 4.470366610295344E-5 30-31 8.940733220590687E-5 0.0 0.0 0.006929068245957783 4.470366610295344E-5 32-33 8.940733220590687E-5 0.0 0.0 0.01922257642426998 4.470366610295344E-5 34-35 8.940733220590687E-5 0.0 0.0 0.038623967512951767 8.940733220590687E-5 36-37 8.940733220590687E-5 4.470366610295344E-5 0.0 0.07125764376810778 8.940733220590687E-5 38-39 1.1175916525738358E-4 8.940733220590687E-5 0.0 0.11504488471595067 8.940733220590687E-5 40-41 1.341109983088603E-4 1.341109983088603E-4 0.0 0.16104495713588976 8.940733220590687E-5 42-43 1.341109983088603E-4 1.341109983088603E-4 0.0 0.20936962019318242 8.940733220590687E-5 44-45 1.7881466441181375E-4 1.341109983088603E-4 0.0 0.273117048055994 8.940733220590687E-5 46-47 1.7881466441181375E-4 1.341109983088603E-4 0.0 0.345648746308036 8.940733220590687E-5 48-49 1.7881466441181375E-4 1.7881466441181375E-4 0.0 0.42432719864923407 8.940733220590687E-5 50-51 1.7881466441181375E-4 1.7881466441181375E-4 0.0 0.5085489055871983 8.940733220590687E-5 52-53 1.7881466441181375E-4 1.7881466441181375E-4 0.0 0.5904236700547575 8.940733220590687E-5 54-55 1.7881466441181375E-4 1.7881466441181375E-4 0.0 0.6786687069419877 8.940733220590687E-5 56-57 1.7881466441181375E-4 1.7881466441181375E-4 0.0 0.7898243727069814 8.940733220590687E-5 58-59 1.7881466441181375E-4 1.7881466441181375E-4 0.0 0.9109489560129337 8.940733220590687E-5 60-61 1.7881466441181375E-4 1.7881466441181375E-4 0.0 1.0372144609207257 8.940733220590687E-5 62-63 2.0116649746329047E-4 1.7881466441181375E-4 0.0 1.1634352621624147 8.940733220590687E-5 64-65 2.235183305147672E-4 1.7881466441181375E-4 0.0 1.2982838709619737 1.5646283136033703E-4 66-67 2.235183305147672E-4 1.7881466441181375E-4 0.0 1.4518633158586702 1.7881466441181375E-4 68-69 2.235183305147672E-4 1.7881466441181375E-4 0.0 1.6218713580482023 1.7881466441181375E-4 70-71 2.235183305147672E-4 1.7881466441181375E-4 0.0 1.8041058529168919 2.0116649746329047E-4 72-73 2.235183305147672E-4 2.0116649746329047E-4 0.0 1.9934705825290027 2.458701635662439E-4 74-75 2.235183305147672E-4 2.235183305147672E-4 0.0 2.1862551425979895 2.682219966177206E-4 76-77 2.235183305147672E-4 2.235183305147672E-4 0.0 2.4011456655548864 2.682219966177206E-4 78-79 2.235183305147672E-4 2.235183305147672E-4 0.0 2.6380750959005397 2.682219966177206E-4 80-81 2.235183305147672E-4 2.235183305147672E-4 0.0 2.900485615924876 2.682219966177206E-4 82-83 2.235183305147672E-4 2.235183305147672E-4 0.0 3.1623820437890293 2.682219966177206E-4 84-85 2.235183305147672E-4 2.235183305147672E-4 0.0 3.4290170602600947 2.682219966177206E-4 86-87 2.235183305147672E-4 2.235183305147672E-4 0.0 3.7096219723883337 2.682219966177206E-4 88-89 2.235183305147672E-4 2.235183305147672E-4 0.0 4.011327014917167 2.682219966177206E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3015 0.0 54.39004 1 GTATCAA 5690 0.0 43.440105 1 ATCAACG 6755 0.0 34.680782 3 CAACGCA 6985 0.0 33.705944 5 TATCAAC 7180 0.0 33.42733 2 TCAACGC 7025 0.0 33.356106 4 AACGCAG 7395 0.0 32.782112 6 ACGCAGA 8090 0.0 28.797813 7 CGCAGAG 8165 0.0 28.533293 8 GTGGTAT 1310 0.0 27.006794 1 GTACTAG 725 0.0 25.718119 1 GCAGAGT 8860 0.0 24.953484 9 GTACATG 6080 0.0 24.848265 1 CTAGCGG 100 0.0016116936 23.856283 3 TACATGG 6080 0.0 23.118843 2 ACATGGG 6015 0.0 21.97237 3 CAGAGTA 8575 0.0 21.429775 10-11 AGAGTAC 8145 0.0 21.364477 10-11 GTATAAG 1195 0.0 21.204134 1 GAGTACT 5630 0.0 20.946136 12-13 >>END_MODULE