FastQCFastQC Report
Sat 18 Jun 2016
SRR1931131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236953
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68320.30541544681537786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57180.25561556277668773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37880.1693374871979876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.057.0230981
GTATCAA63500.042.1720471
ATCAACG78350.033.7004743
CAACGCA79950.033.14415
TCAACGC80450.032.8790784
TATCAAC81750.032.4150432
AACGCAG85050.031.2682956
ACGCAGA97850.026.6441577
GTGGTAT15000.026.319451
CGCAGAG98900.025.6890478
TGGTATC16000.024.6353262
GCAGAGT110600.022.3703679
GTACATG62800.020.9802231
CAGAGTA106100.020.58888810-11
GAGTACT73350.020.39393212-13
GTCGACG1404.86149E-420.352319
AGAGTAC100850.019.63589310-11
TACATGG63950.019.5305922
GTACTTT77750.019.23980314-15
ACATGGG63650.019.174983
Produced by FastQC (version 0.11.5)