FastQCFastQC Report
Sat 18 Jun 2016
SRR1931130_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931130_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584330
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60050.23236196615757276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45230.1750163485313408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26220.1014576311848719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.055.764551
GTATCAA60550.040.3983841
TCAACGC73000.032.4971584
CAACGCA72600.032.487065
ATCAACG71950.032.434363
TATCAAC77250.031.2932072
AACGCAG76450.031.1390616
ACGCAGA85600.027.3722867
CGCAGAG87350.026.6036828
GTACATG68700.023.6211011
GCAGAGT95450.023.0520369
GAGTACT56650.022.3209312-13
GTGGTAT16350.022.2511791
TACATGG69700.022.183342
ACATGGG70850.021.2176843
CAGAGTA96100.020.52263510-11
TGGTATC17150.019.8176172
AGAGTAC88200.019.55899210-11
GTACTTT66500.019.30424114-15
CATGGGG48350.017.2764074
Produced by FastQC (version 0.11.5)