FastQCFastQC Report
Sat 18 Jun 2016
SRR1931130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584330
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74990.29017192076863246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61270.23708272550332196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.15280556275707824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.055.8078461
GTATCAA66300.040.0465551
ATCAACG81150.031.951463
CAACGCA84500.030.8527455
TCAACGC84300.030.8132784
TATCAAC88050.029.7711942
AACGCAG89750.029.10096
GTGGTAT16050.027.274531
ACGCAGA104250.024.688887
CGCAGAG107300.023.6773038
TGGTATC18350.023.032472
GCAGAGT116500.021.114569
GAGTACT72950.020.89574812-13
GTACATG70900.020.4019581
AGAGTAC107200.019.26959810-11
CAGAGTA116150.019.23616810-11
TACATGG72750.018.9300422
ACATGGG71650.018.8229943
GTACTTT83850.018.2077414-15
AGTACTT80450.017.50156212-13
Produced by FastQC (version 0.11.5)