FastQCFastQC Report
Sat 18 Jun 2016
SRR1931129_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931129_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2427938
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45980.18937880621333825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35390.14576154745302394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.052.852721
GTATCAA49700.040.458871
TCAACGC57200.033.9901854
ATCAACG56800.033.8854063
CAACGCA57900.033.5419925
AACGCAG61000.032.1289336
TATCAAC61850.031.8924122
ACGCAGA69200.027.779857
CGCAGAG68950.027.3974958
GCAGAGT77950.022.8302239
GTGGTAT13350.020.8002031
GAGTACT52800.020.68786412-13
CAGAGTA78250.020.27949510-11
TGGTATC13100.020.1011682
GTACATG52200.019.8108961
AGAGTAC70800.019.5571410-11
TACATGG50450.019.4546132
GTACTTT60300.018.26467514-15
ACATGGG51050.017.962283
TAGACCG2806.984792E-717.0000155
Produced by FastQC (version 0.11.5)